The impact of ischemic stroke on bone marrow microenvironment and extracellular vesicles: A study on inflammatory and molecular changes

Experimental Neurology, Год журнала: 2024, Номер 379, С. 114867 - 114867

Опубликована: Июнь 22, 2024

An ischemic stroke (IS) is caused due to the lack of blood flow cerebral tissue. Most studies have focused on how affects localized tissue, but it has been observed that a can cause secondary complications in distant organs, such as Bone Marrow (BM). Our study effect strokes bone marrow microenvironment. (BM) vital organ maintains inflammatory homeostasis and aids repair damaged tissue after injury/IS. We used middle artery occlusion (MCAO) model adult mice (6 months) investigated changes BM environment. cells were for western blot RT-PCR, supernatant was cytokine analysis extracellular vesicle (EVs) isolation. significant increase total cell number within an TNF-alpha MCP-1, which are known inducing pro-inflammatory Western blots whole lysate demonstrated elevated levels factors (IL-6, TNF-alpha, TLR-4) senescence markers (p21 p16). EVs isolated from showed no change size or concentration; however, we found carried increased miRNA-141-3p miRNA-34a. Proteomic BM-derived alteration protein cargo IS. FgB, C3, Fn1, Tra2b levels. The signaling pathway mitochondrial function most affected marrow. IS induces environment secreted BM.

Язык: Английский

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Progress in Research on the Formation Mechanism, Chemical Composition, Pharmacological Effects, and Clinical Applications of Musk: A Review

Journal of Ethnopharmacology, Год журнала: 2025, Номер 344, С. 119512 - 119512

Опубликована: Фев. 17, 2025

Язык: Английский

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Volatile Compounds in Musk and Their Anti-Stroke Mechanisms

Metabolites, Год журнала: 2025, Номер 15(3), С. 181 - 181

Опубликована: Март 7, 2025

Background/Objectives: Musk is a widely used traditional Chinese medicine derived from musk deer that has the pharmacological effects of “activating blood dredging collateral” and “consciousness-restoring resuscitation”. Its volatile compounds (VCs) play key role in these effects, especially treatment stroke. However, there have been no comprehensive studies on differences VCs different musks. This study investigated musks potential targets mechanisms action for Methods: Different were studied via GC–MS, associated with stroke using network pharmacology. Results: A total 99 detected 79 samples. The most important colours forms muscone, phenol, acetic acid, isovaleric acid. Further revealed change organic acids ketones was cause significant difference between white other types musk. In addition, analyses identified 180 major stroke, five (SRC, EGFR, ESR1, PTGS2, DRD2). Enrichment analysis showed an neural related pathways. molecular docking results confirmed can effectively bind main (muscone phenol). Conclusions: These findings provide valuable insights into distinct compositions various underscore treatment.

Язык: Английский

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Traditional Chinese Medicine in Cerebral Infarction: Integrative Strategies and Future Directions

Phytomedicine, Год журнала: 2025, Номер unknown, С. 156841 - 156841

Опубликована: Май 1, 2025

Язык: Английский

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Beyond boundaries: The therapeutic potential of exosomes in neural microenvironments in neurological disorders

Neuroscience, Год журнала: 2024, Номер 553, С. 98 - 109

Опубликована: Июль 2, 2024

Язык: Английский

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Exosomes and tissue engineering: A novel therapeutic strategy for nerve regenerative

Tissue and Cell, Год журнала: 2024, Номер 93, С. 102676 - 102676

Опубликована: Дек. 13, 2024

Язык: Английский

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