Design of fully synthetic signal peptide library and its use for enhanced secretory production of recombinant proteins in Corynebacterium glutamicum DOI Creative Commons
Eun Jung Jeon,

Seong Min Lee,

Hee Soo Hong

и другие.

Microbial Cell Factories, Год журнала: 2024, Номер 23(1)

Опубликована: Сен. 16, 2024

Corynebacterium glutamicum is an attractive host for secretory production of recombinant proteins, including high-value industrial enzymes and therapeutic proteins. The choice appropriate signaling peptide crucial efficient protein secretion. However, due to the limited availability signal peptides in C. glutamicum, establishing optimal secretion system challenging.

Язык: Английский

Cell-free systems: A synthetic biology tool for rapid prototyping in metabolic engineering DOI

Kumyoung Jeung,

Minsun Kim,

Eunsoo Jang

и другие.

Biotechnology Advances, Год журнала: 2025, Номер unknown, С. 108522 - 108522

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

1
Recent advances in the Design-Build-Test-Learn (DBTL) cycle for systems metabolic engineering of Corynebacterium glutamicum DOI

Subeen Jeon,

Yu Jung Sohn,

Haeyoung Lee

и другие.

The Journal of Microbiology, Год журнала: 2025, Номер 63(3), С. e2501021 - e2501021

Опубликована: Март 28, 2025

Язык: Английский

Процитировано

1
Metabolic/Genetic Engineering Approaches for Lignocellulosic Biomass and Enzymes DOI
Hrithik Dey,

S.S. Paul,

Meenakshi Kaira

и другие.

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
A High-Throughput Cell-Free Enzyme Screening System Using Redox-Responsive Hydrogel Beads as Artificial Compartments DOI

Taisei Koga,

Yui Okawa,

Tomoyuki Ito

и другие.

ACS Synthetic Biology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 13, 2025

We have developed a rapid, simple, and high-throughput screening system for recombinant enzymes using disulfide-bonded hydrogel beads (HBs) produced via microfluidic method. These redox-responsive HBs were compatible with the biosynthesis of enzyme mutants cell-free protein synthesis, fluorescent staining through an enzymatic reaction, genetic information recovery after fluorescence-activated droplet sorting (FADS). The expression microbial transglutaminase zymogen (MTGz) synthesis cross-linking-reactivity-based product validated. Next-generation sequencing (NGS) analysis genes recovered from highly identified novel mutation sites (N25 N27) in propeptide domain. introduction these mutations allowed design engineered active MTGz, demonstrating potential as artificial compartments FADS-based selection that catalyze peptide cross-linking reactions.

Язык: Английский

Процитировано

0
A Co-culture of Staphylococcus epidermidis and Staphylococcus aureus in a Monodisperse Droplet to Investigate Microbial Interaction at Defined Microenvironment DOI
Sungyup Jung,

Kyung Han Kim,

Jae Seong Kim

и другие.

Korean Journal of Chemical Engineering, Год журнала: 2025, Номер unknown

Опубликована: Май 7, 2025

Язык: Английский

Процитировано

0
Accelerating enzyme discovery and engineering with high-throughput screening DOI Creative Commons
Eray Ulaş Bozkurt, Emil C. Ørsted, Daniel C. Volke

и другие.

Natural Product Reports, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Recent progress in the DBTL cycle, including machine learning, facilitated enzyme mining for biocatalysis. Automation and standardization of library construction, coupled to high-throughput screening, further accelerates discovery process.

Язык: Английский

Процитировано

2
Design of fully synthetic signal peptide library and its use for enhanced secretory production of recombinant proteins in Corynebacterium glutamicum DOI Creative Commons
Eun Jung Jeon,

Seong Min Lee,

Hee Soo Hong

и другие.

Microbial Cell Factories, Год журнала: 2024, Номер 23(1)

Опубликована: Сен. 16, 2024

Corynebacterium glutamicum is an attractive host for secretory production of recombinant proteins, including high-value industrial enzymes and therapeutic proteins. The choice appropriate signaling peptide crucial efficient protein secretion. However, due to the limited availability signal peptides in C. glutamicum, establishing optimal secretion system challenging.

Язык: Английский

Процитировано

0