Design of fully synthetic signal peptide library and its use for enhanced secretory production of recombinant proteins in Corynebacterium glutamicum DOI Creative Commons
Eun Jung Jeon,

Seong Min Lee,

Hee Soo Hong

et al.

Microbial Cell Factories, Journal Year: 2024, Volume and Issue: 23(1)

Published: Sept. 16, 2024

Corynebacterium glutamicum is an attractive host for secretory production of recombinant proteins, including high-value industrial enzymes and therapeutic proteins. The choice appropriate signaling peptide crucial efficient protein secretion. However, due to the limited availability signal peptides in C. glutamicum, establishing optimal secretion system challenging.

Language: Английский

Cell-free systems: A synthetic biology tool for rapid prototyping in metabolic engineering DOI

Kumyoung Jeung,

Minsun Kim,

Eunsoo Jang

et al.

Biotechnology Advances, Journal Year: 2025, Volume and Issue: unknown, P. 108522 - 108522

Published: Jan. 1, 2025

Language: Английский

Citations

1
Recent advances in the Design-Build-Test-Learn (DBTL) cycle for systems metabolic engineering of Corynebacterium glutamicum DOI

Subeen Jeon,

Yu Jung Sohn,

Haeyoung Lee

et al.

The Journal of Microbiology, Journal Year: 2025, Volume and Issue: 63(3), P. e2501021 - e2501021

Published: March 28, 2025

Language: Английский

Citations

1
Metabolic/Genetic Engineering Approaches for Lignocellulosic Biomass and Enzymes DOI
Hrithik Dey,

S.S. Paul,

Meenakshi Kaira

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
A High-Throughput Cell-Free Enzyme Screening System Using Redox-Responsive Hydrogel Beads as Artificial Compartments DOI

Taisei Koga,

Yui Okawa,

Tomoyuki Ito

et al.

ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

We have developed a rapid, simple, and high-throughput screening system for recombinant enzymes using disulfide-bonded hydrogel beads (HBs) produced via microfluidic method. These redox-responsive HBs were compatible with the biosynthesis of enzyme mutants cell-free protein synthesis, fluorescent staining through an enzymatic reaction, genetic information recovery after fluorescence-activated droplet sorting (FADS). The expression microbial transglutaminase zymogen (MTGz) synthesis cross-linking-reactivity-based product validated. Next-generation sequencing (NGS) analysis genes recovered from highly identified novel mutation sites (N25 N27) in propeptide domain. introduction these mutations allowed design engineered active MTGz, demonstrating potential as artificial compartments FADS-based selection that catalyze peptide cross-linking reactions.

Language: Английский

Citations

0
A Co-culture of Staphylococcus epidermidis and Staphylococcus aureus in a Monodisperse Droplet to Investigate Microbial Interaction at Defined Microenvironment DOI
Sungyup Jung,

Kyung Han Kim,

Jae Seong Kim

et al.

Korean Journal of Chemical Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: May 7, 2025

Language: Английский

Citations

0
Accelerating enzyme discovery and engineering with high-throughput screening DOI Creative Commons
Eray Ulaş Bozkurt, Emil C. Ørsted, Daniel C. Volke

et al.

Natural Product Reports, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Recent progress in the DBTL cycle, including machine learning, facilitated enzyme mining for biocatalysis. Automation and standardization of library construction, coupled to high-throughput screening, further accelerates discovery process.

Language: Английский

Citations

2
Design of fully synthetic signal peptide library and its use for enhanced secretory production of recombinant proteins in Corynebacterium glutamicum DOI Creative Commons
Eun Jung Jeon,

Seong Min Lee,

Hee Soo Hong

et al.

Microbial Cell Factories, Journal Year: 2024, Volume and Issue: 23(1)

Published: Sept. 16, 2024

Corynebacterium glutamicum is an attractive host for secretory production of recombinant proteins, including high-value industrial enzymes and therapeutic proteins. The choice appropriate signaling peptide crucial efficient protein secretion. However, due to the limited availability signal peptides in C. glutamicum, establishing optimal secretion system challenging.

Language: Английский

Citations

0