The Neuroprotective Effect of Short-chain Fatty Acids Against Hypoxia-reperfusion Injury DOI Creative Commons

Anjit K. Harijan,

Retnamony Kalaiarasan,

Amit Ghosh

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Дек. 23, 2023

Abstract Gut microbe-derived short-chain fatty acids (SCFAs) are known to have a profound impact on various brain functions, including cognition, mood, and overall neurological health. However, their role, if any, in protecting against hypoxic injury ischemic stroke has not been extensively studied. In this study, we investigated the effects of two major SCFAs abundant gut, propionate (P) butyrate (B), hypoxia-reperfusion using neuronal cell line zebrafish model. Neuro 2a (N2a) cells treated with P B exhibited reduced levels mitochondrial cytosolic reactive oxygen species (ROS), diminished loss membrane potential, suppressed caspase activation, lower rates death when exposed CoCl 2 -induced hypoxia, compared control group. Furthermore, adult fed SCFAs-supplemented feeds showed less susceptibility conditions group, as indicated by multiple behavioral measures. Histological analysis TTC-stained sections revealed lesser damage SCFAs-fed We also found that FABP7 (also BLBP), neuroprotective acid binding protein, was upregulated brains Additionally, overexpressed N2a cells, it protected from injury. Overall, our data clearly demonstrates role suggests potential dietary supplementation mitigate stroke-induced damage. Highlights Short-chain (SCFA) Propionate Butyrate (B) protect hypoxia-reperfusion. Zebrafish, an SCFA-supplemented diet, more resilient diet boost expression (fatty protein). overexpression provides protection reduce (ROS) increase FABP7, contributing neuroprotection.

Язык: Английский

Melatonin Ameliorates Abnormal Sleep-Wake Behavior via Facilitating Lipid Metabolism in a Zebrafish Model of Parkinson’s Disease DOI

Meng‐Zhu Pang,

Han-Xing Li,

Xue-Qin Dai

и другие.

Neuroscience Bulletin, Год журнала: 2024, Номер 40(12), С. 1901 - 1914

Опубликована: Сен. 16, 2024

Язык: Английский

Процитировано

0
The Neuroprotective Effect of Short-chain Fatty Acids Against Hypoxia-reperfusion Injury DOI Creative Commons

Anjit K. Harijan,

Retnamony Kalaiarasan,

Amit Ghosh

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Дек. 23, 2023

Abstract Gut microbe-derived short-chain fatty acids (SCFAs) are known to have a profound impact on various brain functions, including cognition, mood, and overall neurological health. However, their role, if any, in protecting against hypoxic injury ischemic stroke has not been extensively studied. In this study, we investigated the effects of two major SCFAs abundant gut, propionate (P) butyrate (B), hypoxia-reperfusion using neuronal cell line zebrafish model. Neuro 2a (N2a) cells treated with P B exhibited reduced levels mitochondrial cytosolic reactive oxygen species (ROS), diminished loss membrane potential, suppressed caspase activation, lower rates death when exposed CoCl 2 -induced hypoxia, compared control group. Furthermore, adult fed SCFAs-supplemented feeds showed less susceptibility conditions group, as indicated by multiple behavioral measures. Histological analysis TTC-stained sections revealed lesser damage SCFAs-fed We also found that FABP7 (also BLBP), neuroprotective acid binding protein, was upregulated brains Additionally, overexpressed N2a cells, it protected from injury. Overall, our data clearly demonstrates role suggests potential dietary supplementation mitigate stroke-induced damage. Highlights Short-chain (SCFA) Propionate Butyrate (B) protect hypoxia-reperfusion. Zebrafish, an SCFA-supplemented diet, more resilient diet boost expression (fatty protein). overexpression provides protection reduce (ROS) increase FABP7, contributing neuroprotection.

Язык: Английский

Процитировано

1