The Neuroprotective Effect of Short-chain Fatty Acids Against Hypoxia-reperfusion Injury DOI Creative Commons

Anjit K. Harijan,

Retnamony Kalaiarasan,

Amit Ghosh

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 23, 2023

Abstract Gut microbe-derived short-chain fatty acids (SCFAs) are known to have a profound impact on various brain functions, including cognition, mood, and overall neurological health. However, their role, if any, in protecting against hypoxic injury ischemic stroke has not been extensively studied. In this study, we investigated the effects of two major SCFAs abundant gut, propionate (P) butyrate (B), hypoxia-reperfusion using neuronal cell line zebrafish model. Neuro 2a (N2a) cells treated with P B exhibited reduced levels mitochondrial cytosolic reactive oxygen species (ROS), diminished loss membrane potential, suppressed caspase activation, lower rates death when exposed CoCl 2 -induced hypoxia, compared control group. Furthermore, adult fed SCFAs-supplemented feeds showed less susceptibility conditions group, as indicated by multiple behavioral measures. Histological analysis TTC-stained sections revealed lesser damage SCFAs-fed We also found that FABP7 (also BLBP), neuroprotective acid binding protein, was upregulated brains Additionally, overexpressed N2a cells, it protected from injury. Overall, our data clearly demonstrates role suggests potential dietary supplementation mitigate stroke-induced damage. Highlights Short-chain (SCFA) Propionate Butyrate (B) protect hypoxia-reperfusion. Zebrafish, an SCFA-supplemented diet, more resilient diet boost expression (fatty protein). overexpression provides protection reduce (ROS) increase FABP7, contributing neuroprotection.

Language: Английский

Melatonin Ameliorates Abnormal Sleep-Wake Behavior via Facilitating Lipid Metabolism in a Zebrafish Model of Parkinson’s Disease DOI

Meng‐Zhu Pang,

Han-Xing Li,

Xue-Qin Dai

et al.

Neuroscience Bulletin, Journal Year: 2024, Volume and Issue: 40(12), P. 1901 - 1914

Published: Sept. 16, 2024

Language: Английский

Citations

0
The Neuroprotective Effect of Short-chain Fatty Acids Against Hypoxia-reperfusion Injury DOI Creative Commons

Anjit K. Harijan,

Retnamony Kalaiarasan,

Amit Ghosh

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 23, 2023

Abstract Gut microbe-derived short-chain fatty acids (SCFAs) are known to have a profound impact on various brain functions, including cognition, mood, and overall neurological health. However, their role, if any, in protecting against hypoxic injury ischemic stroke has not been extensively studied. In this study, we investigated the effects of two major SCFAs abundant gut, propionate (P) butyrate (B), hypoxia-reperfusion using neuronal cell line zebrafish model. Neuro 2a (N2a) cells treated with P B exhibited reduced levels mitochondrial cytosolic reactive oxygen species (ROS), diminished loss membrane potential, suppressed caspase activation, lower rates death when exposed CoCl 2 -induced hypoxia, compared control group. Furthermore, adult fed SCFAs-supplemented feeds showed less susceptibility conditions group, as indicated by multiple behavioral measures. Histological analysis TTC-stained sections revealed lesser damage SCFAs-fed We also found that FABP7 (also BLBP), neuroprotective acid binding protein, was upregulated brains Additionally, overexpressed N2a cells, it protected from injury. Overall, our data clearly demonstrates role suggests potential dietary supplementation mitigate stroke-induced damage. Highlights Short-chain (SCFA) Propionate Butyrate (B) protect hypoxia-reperfusion. Zebrafish, an SCFA-supplemented diet, more resilient diet boost expression (fatty protein). overexpression provides protection reduce (ROS) increase FABP7, contributing neuroprotection.

Language: Английский

Citations

1