Thymol as a control factor of the expression of key genes of the ginsenoside biosynthesis pathway and its effect on the production of ginseng saponins in Panax quinquefolium hairy root cultures

Industrial Crops and Products, Год журнала: 2024, Номер 210, С. 118151 - 118151

Опубликована: Янв. 31, 2024

Язык: Английский

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In Vitro Cultivation and Ginsenosides Accumulation in Panax ginseng: A Review

Plants, Год журнала: 2023, Номер 12(17), С. 3165 - 3165

Опубликована: Сен. 3, 2023

The use of in vitro tissue culture for herbal medicines has been recognized as a valuable source botanical secondary metabolites. ginseng species is used the production bioactive compounds such phenolics, polysaccharides, and especially ginsenosides, which are utilized food, cosmetics, pharmaceutical industries. This review paper focuses on Panax accumulation ginsenosides. In applied to study organogenesis biomass culture, involved direct rooting shooting from explants indirect morphogenesis somatic embryogenesis via callus, mass disorganized cells. Biomass was conducted with different types cultures, adventitious roots, cell suspension, hairy subsequently large scale bioreactor. provides cumulative knowledge biotechnological methods increase ginsenoside resources P. ginseng. addition, ginsenosides summarized at enhanced levels activity content elicitor treatment, together perspectives new breeding tools can be developed future.

Язык: Английский

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Analysis of the Synergistic Effect of Endophytic Bacteria and Metabolites on Yield in Soybean Roots Under Biochar with Nitrogen Fertilization

Agronomy, Год журнала: 2025, Номер 15(1), С. 177 - 177

Опубликована: Янв. 13, 2025

The appropriate application of nitrogen fertilizers and biochar to the soil is beneficial for soybean growth, a combination can improve low-nitrogen fertilizer utilization efficiency. However, effect on roots still unclear. Therefore, in this study, we investigate changes endophytic bacterial communities metabolites under different combinations treatments by setting fertilization levels using high-throughput sequencing non-targeted metabolomics techniques. results showed that applying 20 t/ha 180 kg/ha (C2N1) resulted highest yield. By studying community roots, it was observed dominant phyla were Proteobacteria, Bacteroidota, Actinobacteriota, genera Bradyrhizobium, Streptomyces, Rhizobacter. Moreover, metabolic pathway analysis revealed glycosylphosphatidylinositol (GPI)-anchor biosynthesis most significant C0N1 vs. C0N0 comparison group, glycerophospholipid metabolism C2N1 C1N1 group; they both associated with regulatory mechanisms such as plant growth promotion nutrient cycling. Structural equation modeling indicates bacteria have positive impact Bradyrhizobium Rhizobium are observable variables effects bacteria, while Phe-Thr 7-allyloxycoumarin metabolites. Endophytic not only direct crop yield, but also indirectly affect yield through their This study has important scientific value guiding significance improving yields.

Язык: Английский

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MicroRNA-mediated regulation of ginsenoside biosynthesis in Panax ginseng and its biotechnological implications

Science Progress, Год журнала: 2025, Номер 108(2)

Опубликована: Март 31, 2025

Panax ginseng , renowned for its therapeutic properties, derives much of medicinal value from ginsenosides, a group bioactive triterpenoid saponins. The biosynthesis ginsenosides is regulated by various mechanisms, including microRNAs (miRNAs), which play key roles in gene regulation. Recent studies have identified numerous miRNAs P . and other plants, highlighting their potential to influence targeting genes the pathway. This mini-review explores current understanding miRNA-mediated regulation discusses controlling ginsenoside production through miRNA manipulation. Although research still primitive, ongoing suggest promising applications agriculture medicine. Further functional on these could provide valuable insights into optimizing enhancing properties.

Язык: Английский

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Targeted preparation of six rare ginsenosides using two β-glucosidases from Bifidobacterium adolescentis

Food Bioscience, Год журнала: 2024, Номер 59, С. 104192 - 104192

Опубликована: Апрель 26, 2024

Язык: Английский

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Comparison of the Transformation Ability of the Major Saponins in Panax notoginseng by Penicillum fimorum Enzyme and Commercial β-glucosidase

Microorganisms, Год журнала: 2025, Номер 13(3), С. 495 - 495

Опубликована: Фев. 23, 2025

Ginsenosides with less sugar groups, which are called minor ginsenosides, might have a greater pharmacological activity and better adsorptive ability, but their content in nature is extremely low. In this study, strain of Penicillium fimorum strong saponin transformation ability was isolated from fresh Gastrodia elata. A comparative biotransformation experiment the major saponins Panax notoginseng root were conducted using crude enzymes P. commercial β-glucosidase to produce ginsenosides. Specifically, enzyme able transform into 13 72 h, while same 15 h. The most significant difference between these two Rb1. To best our knowledge, reported here for first time. These potential improve economic value expand methods preparing by transforming total root.

Язык: Английский

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Phylogenetic Analysis and Expression Patterns of Triterpenoid Saponin Biosynthesis Genes in 19 Araliaceae Plants

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 3439 - 3439

Опубликована: Апрель 7, 2025

The Araliaceae family has significant economic and medicinal value. However, the phylogenetic relationships expression patterns of key genes active triterpenoid substance within this are still unclear. In study, we employed comparative transcriptomics to analyze transcriptomes 19 species from 11 genera Araliaceae, aiming elucidate evolutionary history in ginsenoside biosynthesis pathway. Our results divide into two subfamilies: Aralioideae Hydrocotyloideae. is further classified three groups: Aralia-Panax group, Polyscias-Pseudopanax Asian Palmate group. PhyloNet analysis reveals that common ancestor Panax ginseng, quinquefolius, japonicus was an allopolyploid, likely resulting hybridization between notoginseng pseudoginseng. Additionally, all underwent pg-β event, which may be critical for biosynthesis. We discovered exhibit distinct enzyme (β-AS, DDS, CYP450, UGTs) compared other species. These show independent lineages gene trees, suggesting unique functional adaptations enable efficiently synthesize ginsenosides. This study provides a theoretical foundation conservation utilization germplasm resources.

Язык: Английский

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The NAC Transcription Factor PgNAC41-2 Gene Involved in the Regulation of Ginsenoside Biosynthesis in Panax ginseng

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(15), С. 11946 - 11946

Опубликована: Июль 26, 2023

Ginseng (

Язык: Английский

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Optimization of saponin extraction from the leaves of Panax notoginseng and Panax quinquefolium and evaluation of their antioxidant, antihypertensive, hypoglycemic and anti-inflammatory activities

Food Chemistry X, Год журнала: 2024, Номер 23, С. 101642 - 101642

Опубликована: Июль 14, 2024

and

Язык: Английский

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Unlocking secrets of nature's chemists: Potential of CRISPR/Cas-based tools in plant metabolic engineering for customized nutraceutical and medicinal profiles

Plant Physiology and Biochemistry, Год журнала: 2023, Номер 203, С. 108070 - 108070

Опубликована: Окт. 1, 2023

Язык: Английский

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