Biophysical Reviews, Год журнала: 2021, Номер 13(6), С. 1245 - 1271
Опубликована: Ноя. 17, 2021
Язык: Английский
Biofabrication, Год журнала: 2021, Номер 14(2), С. 022001 - 022001
Опубликована: Ноя. 16, 2021
Droplet-based microfluidic systems have been employed to manipulate discrete fluid volumes with immiscible phases. Creating the droplets at microscale has led a paradigm shift in mixing, sorting, encapsulation, sensing, and designing high throughput devices for biomedical applications. Droplet microfluidics opened many opportunities microparticle synthesis, molecular detection, diagnostics, drug delivery, cell biology. In present review, we first introduce standard methods droplet generation (i.e. passive active methods) discuss latest examples of emulsification particle synthesis approaches enabled by platforms. Then, applications droplet-based different are detailed. Finally, general overview trends along perspectives future potentials field provided.
Язык: Английский
Процитировано
114
Chemical Reviews, Год журнала: 2022, Номер 122(7), С. 7061 - 7096
Опубликована: Фев. 18, 2022
Cell manipulation in droplets has emerged as one of the great successes microfluidic technologies, with development single-cell screening. However, droplet format also served to go beyond studies, namely by considering interactions between different cells or and their physical chemical environment. These studies pose specific challenges linked need for long-term culture adherent diverse types measurements associated complex biological phenomena. Here we review emergence methods culturing studying interactions. We begin characterizing quantitative aspects that determine ability encapsulate cells, transport molecules, provide sufficient nutrients within droplets. This is followed an evaluation constraints such control biochemical environment promoting anchorage cells. first part ends a description measurement have been developed. The second manuscript focuses on applications these technologies cancer immunology, stem while paying special attention relevance cellular assays providing guidelines improving this relevance.
Язык: Английский
Процитировано
95
Nature Communications, Год журнала: 2020, Номер 11(1)
Опубликована: Ноя. 25, 2020
The spontaneous self-assembly of multicellular ensembles into living materials with synergistic structure and function remains a considerable challenge in biotechnology synthetic biology. Here, we exploit the aqueous two-phase separation dextran-in-PEG emulsion micro-droplets for capture, spatial organization immobilization algal cells or algal/bacterial cell communities to produce discrete spheroids capable both aerobic (oxygen producing) hypoxic (hydrogen photosynthesis daylight under air. We show that localized oxygen depletion results hydrogen production from core microscale reactor, demonstrate enhanced levels evolution can be achieved synergistically by spontaneously enclosing photosynthetic within shell bacterial undergoing respiration. Our highlight promising droplet-based environmentally benign approach dispersible microbial micro-reactors comprising segregated cellular micro-niches dual functionality, provide step towards photobiological conditions.
Язык: Английский
Процитировано
84
Materials & Design, Год журнала: 2022, Номер 223, С. 111230 - 111230
Опубликована: Окт. 5, 2022
In the last two decades, microfluidics has emerged as a powerful tool of high precision and versatility, mainly applied to biomedicine. As result its strengths, literature concerning fundamental principles is comprehensive. Since this field continues evolve, new areas, such production solid microcapsules, need be covered updated. To date, some specific reviews cover microencapsulation by microfluidic focusing on area biology medicine: microgels, controlled drug release, bacteria cell encapsulation, tissue engineering. Nonetheless, technique expanding other research areas like material science, which can bring these materials applications food cosmetics, self-healing materials, energy storage industry. This work aims aspects presenting latest trends in microcapsule production. The review traces field's evolution, showing weaknesses challenges faced scientists fully exploit advantages technique. end, focuses different facets science: core shell materials; parameters affecting capsule structure; curing processes; performance; mass challenges. initially addresses fundamentals required understand formation process.
Язык: Английский
Процитировано
58
International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(18), С. 14349 - 14349
Опубликована: Сен. 20, 2023
Spinal cord injuries (SCIs) can lead to significant neurological deficits and lifelong disability, with far-reaching physical, psychological, economic consequences for affected individuals their families. Current treatments SCIs are limited in ability restore function, there is a pressing need innovative therapeutic approaches. Stem cell therapy has emerged as promising strategy promote the regeneration repair of damaged neural tissue following SCIs. This review article comprehensively discusses potential different stem types, such embryonic cells (ESCs), induced pluripotent (iPSCs), mesenchymal (MSCs), stem/progenitor (NSPCs), SCI treatment. We provide an in-depth analysis unique advantages challenges associated each type, well latest advancements field. Furthermore, we address critical faced SCIs, including safety concerns, ethical considerations, standardization protocols, optimization transplantation parameters, development effective outcome measures. also discuss integration novel technologies gene editing, biomaterials, engineering enhance cells. The concludes by emphasizing importance collaborative efforts among various stakeholders scientific community, researchers, clinicians, bioengineers, industry partners, patients, overcome these realize full patients. By fostering collaborations advancing our understanding biology regenerative medicine, pave way groundbreaking therapies that improve lives those
Язык: Английский
Процитировано
32
Microfluidics and Nanofluidics, Год журнала: 2025, Номер 29(3)
Опубликована: Фев. 18, 2025
Язык: Английский
Процитировано
1
Biofabrication, Год журнала: 2023, Номер 15(3), С. 032003 - 032003
Опубликована: Май 22, 2023
Abstract Soft tissue defects are a common clinical challenge mostly caused by trauma, congenital anomalies and oncological surgery. Current soft reconstruction options include synthetic materials (fillers implants) autologous adipose transplantation through flap surgery and/or lipotransfer. Both reconstructive hold important disadvantages to which vascularized engineering (VATE) strategies could offer solutions. In this review, we first summarized pivotal characteristics of functional such as the structure, function, cell types, development extracellular matrix (ECM). Next, discussed relevant sources how they applied in different state-of-the-art VATE techniques. Herein, biomaterial scaffolds hydrogels, ECMs, spheroids, organoids, sheets, three dimensional printing microfluidics overviewed. Also, included vesicles emphasized their potential role VATE. Lastly, current challenges future perspectives pointed out help pave road towards applications.
Язык: Английский
Процитировано
16
Biophysical Reviews, Год журнала: 2021, Номер 13(5), С. 769 - 786
Опубликована: Июль 14, 2021
Язык: Английский
Процитировано
27
IBRO Neuroscience Reports, Год журнала: 2025, Номер 18, С. 679 - 689
Опубликована: Апрель 17, 2025
Neurodevelopmental studies employing animal models encounter challenges due to interspecies differences and ethical concerns. Maturing neurons of human origin, undergoing several developmental stages, present a powerful alternative. In this study, embryonic stem cell (H9 line) was differentiated into neural cells subsequently matured over 30 days. Ion AmpliSeq™ used for transcriptomic characterization cell-derived at multiple time points. Data analysis revealed progressive increase markers associated with neuronal development astrocyte markers, indicating the establishment co-culture accommodating both glial neurons. Transcriptomic pathway enrichment also more pronounced GABAergic phenotype in neurons, signifying their specialization toward type. The findings confirm robustness these across different passages demonstrate detailed progression through stages development. model is intended neurodevelopmental applications can be adapted investigate how genetic modifications or exposure chemicals, pharmaceuticals, other environmental factors influence maturation.
Язык: Английский
Процитировано
0
Bioengineered, Год журнала: 2020, Номер 11(1), С. 1137 - 1145
Опубликована: Янв. 1, 2020
Staphylococcus aureus is a common foodborne pathogenic microorganism which can cause food poisoning and it to both humans animals. Therefore, rapid detection of S. infection great significance. In this study, microfluidic platform was introduced detect by fluorescence labeling method self-made chip, has immune spheres were used study the effect capturing aureus. Through experiment, we found that be for microbial culture, antibody coated on diameter 50 ~ 90 μm microspheres detection. On premise optimizing sample flow rate time, bacterial quantitatively monitored. Results showed our at injection 5 μL·min−1 reacted 4 min limit bacteria 1.5 × 101 CFU/μL. However, time traditional 24 hs 72 hs, operation complex cumbersome. These findings indicated chip in portable, sensitive, accurate, laying good foundation further research application platform.
Язык: Английский
Процитировано
21