Bioprinting,
Год журнала:
2022,
Номер
26, С. e00202 - e00202
Опубликована: Март 9, 2022
Organ-on-chip
(OoC)
platforms
are
a
new
class
of
advanced
microfluidic
devices
that
have
been
attracting
significant
attention
for
drug
development
since
they
can
allow
faster
and
more
patient
specific
evaluation
the
effects
drugs
due
to
mimicking
organ
human
models.
As
most
devices,
great
majority
OoC
is
made
from
polydimethylsiloxane
(PDMS),
easiness
handle
its
physicochemical
properties.
Nevertheless,
PDMS
leach
uncrosslinked
oligomer
absorb
small
hydrophobic
molecules,
which
makes
it
less
suitable
cell
culture.
In
this
work,
we
review
different
kinds
materials
used
together
with,
or
in
replacement,
manufacture
platforms.
Several
studies
replicated
differentiated
structures
their
key
physiological
functions,
such
as
muscle
contraction,
skin
elasticity
deformability,
gas
exchange
alveolar
sacs,
electrical
stimulation
even
monitor
microenvironment,
inside
chip.
Therefore,
Review,
recent
advances
on
platforms,
including
fabrication
miniaturization
biosensors
an
end-use
perspective
revised.
Advanced Materials Technologies,
Год журнала:
2022,
Номер
7(7)
Опубликована: Фев. 12, 2022
Abstract
Cardiovascular
disease
morbidity
has
increased
worldwide.
Organs‐on‐chips
and
human
pluripotent
stem
cell
(hPSC)
technologies
aid
to
overcome
some
of
the
limitations
in
cardiac
vitro
models.
Here,
a
bi‐compartmental,
monolithic
heart‐on‐chip
device
that
facilitates
porous
membrane
integration
single
fabrication
step
is
presented.
Moreover,
includes
open‐top
compartments
allow
facile
co‐culture
hPSC‐derived
cardiomyocytes
adult
fibroblast
into
geometrically
defined
microtissues.
The
can
be
reversibly
closed
with
glass
seal
or
lid
fully
customized
3D‐printed
pyrolytic
carbon
electrodes
allowing
electrical
stimulation
A
subjacent
microfluidic
channel
allowed
localized
dynamic
drug
administration
microtissues,
as
demonstrated
by
chronotropic
response
isoprenaline.
also
populated
induced
stem‐derived
endothelial
cells
heterotypic
one
device.
Overall,
this
study
demonstrates
novel
model
systematically
integrates
an
3D
printed
electrode
for
pacing
culture
tissues
while
enabling
active
perfusion
dosing.
Advances
engineering
models
represent
important
towards
making
organ‐on‐a‐chip
technology
routine
aspect
preclinical
development.
The
role
of
the
circulatory
system,
containing
blood
and
lymphatic
vasculatures,
within
body,
has
become
increasingly
focused
on
by
researchers
as
dysfunction
either
systems
been
linked
to
serious
complications
disease.
Currently,
Biomedicine & Pharmacotherapy,
Год журнала:
2021,
Номер
146, С. 112584 - 112584
Опубликована: Дек. 27, 2021
Heart
muscle
injury
and
an
elevated
troponin
level
signify
myocardial
infarction
(MI),
which
may
result
in
defective
uncoordinated
segments,
reduced
cardiac
output,
ultimately,
death.
Physicians
apply
thrombolytic
therapy,
coronary
artery
bypass
graft
(CABG)
surgery,
or
percutaneous
intervention
(PCI)
to
recanalize
restore
blood
flow
the
arteries,
albeit
they
were
not
convincingly
able
solve
heart
problems.
Thus,
researchers
aim
introduce
novel
substitutional
therapies
for
regenerating
functionalizing
damaged
tissue
based
on
engineering
concepts.
Cell-based
approaches,
utilizing
biomaterials,
gene,
drug,
growth
factor
delivery
systems,
are
most
leading
studies
field
of
regeneration.
Also,
understanding
primary
cause
MI
thus
selecting
efficient
treatment
method
can
be
enhanced
by
preparing
microdevices
so-called
heart-on-a-chip.
In
this
regard,
microfluidic
approaches
used
as
diagnostic
platforms
drug
screening
disease
treatment.
Additionally,
bioprinting
technique
with
whole
organ
3D
printing
human
major
vessels,
cardiomyocytes
endothelial
cells
ideal
goal
remarkable
achievement
near
future.
Consequently,
review
discusses
different
aspects,
advancements,
challenges
mentioned
methods
presenting
advantages
disadvantages,
chronological
indications,
application
prospects
various
therapeutic
approaches.
Advanced Healthcare Materials,
Год журнала:
2024,
Номер
13(20)
Опубликована: Март 27, 2024
Abstract
In
vitro
blood
vessel
models
are
significant
for
disease
modeling,
drug
assays,
and
therapeutic
development.
Microfluidic
technologies
allow
to
create
physiologically
relevant
culture
reproducing
the
features
of
in
vivo
vascular
microenvironment.
However,
current
microfluidic
limited
by
impractical
rectangular
cross‐sections
single
or
nonsynchronous
compound
mechanical
stimuli.
This
study
proposes
a
new
strategy
creating
round‐shaped
deformable
soft
channels
serve
as
artificial
vasculature
developing
with
physio‐mechanical
microenvironments.
Endothelial
cells
seeded
into
used
assess
effects
remodeled
environment.
Furthermore,
3D
stenosis
model
is
constructed
recapitulate
flow
disturbances
atherosclerosis.
Soft
microchannels
can
also
be
integrated
traditional
microfluidics
realize
multifunctional
composite
systems.
technology
provides
insights
applying
chips
prospective
approach
constructing
models.
Micromachines,
Год журнала:
2025,
Номер
16(1), С. 93 - 93
Опубликована: Янв. 15, 2025
Microfluidic-based
cell-stretching
devices
are
vital
for
studying
the
molecular
pathways
involved
in
cellular
responses
to
mechanobiological
processes.
Accurate
evaluation
of
these
requires
detailed
observation
cells
cultured
this
device.
This
study
aimed
develop
a
method
preparing
microscope
slides
enable
high-magnification
imaging
devices.
The
key
innovation
is
creating
peelable
bond
between
cell
culture
membrane
and
upper
channel,
allowing
easy
removal
layer
precise
cutting
microscopy.
Using
fabricated
device,
OP9
(15,000
cells/channel)
were
stretched,
effects
focal
adhesion
proteins
intracellular
distribution
YAP1
examined
under
fluorescence
with
100×
60×
objectives.
Stretch
stimulation
increased
integrinβ1
expression
promoted
integrin–vinculin
complex
formation
by
approximately
1.4-fold
cells.
Furthermore,
nuclear
localization
was
significantly
enhanced
(approximately
1.3-fold)
during
stretching.
offers
valuable
tool
researchers
using
microfluidic-based
advancement
techniques
microdevice
research
expected
further
drive
progress
mechanobiology
research.
