SARS-CoV-2 concentration in wastewater consistently predicts trends in COVID-19 case counts by at least two days across multiple WWTP scales DOI Creative Commons
Candice L. Swift, Mirza Isanovic, Karlen E. Correa Velez

и другие.

Environmental Advances, Год журнала: 2023, Номер 11, С. 100347 - 100347

Опубликована: Янв. 25, 2023

Wastewater surveillance of SARS-CoV-2 has proven instrumental in mitigating the spread COVID-19 by providing an economical and equitable approach to disease surveillance. Here, we analyze correlation RNA influents seven wastewater plants (WWTPs) across state South Carolina with corresponding daily case counts determine whether underlying characteristics WWTPs sewershed populations predict stronger correlations. The served these have varying social vulnerability represent 24% population. study spanned 15 months from April 19, 2020, July 1, 2021, which includes administration first vaccines. concentrations were measured either reverse transcription quantitative PCR (RT-qPCR) or droplet digital (RT-ddPCR). Although average flow rate varied WWTPs, strongest was identified for six when lagged two days after concentration wastewater. weakest found WWTP 6, had lowest ratio population rate, indicating that signal too dilute a robust correlation. Smoothing 7-day moving improved strength between while dampening effect lag-time optimization. Correlation cases compared determined at ZIP-code levels. correlations using ZIP-code-level versus sewershed-level not statistically different WWTPs. Results indicate surveillance, even without normalization fecal indicators, is strong predictor clinical least days, especially RT-ddPCR. Furthermore, may be useful metric assess suitable program.

Язык: Английский

Wastewater-based epidemiology for COVID-19 surveillance and beyond: A survey DOI Creative Commons
Chen Chen,

Yunfan Wang,

Gursharn Kaur

и другие.

Epidemics, Год журнала: 2024, Номер 49, С. 100793 - 100793

Опубликована: Сен. 26, 2024

Язык: Английский

Процитировано

6
Evaluation of viral concentration and extraction methods for SARS-CoV-2 recovery from wastewater using droplet digital and quantitative RT-PCR DOI Creative Commons

Lampros Dimitrakopoulos,

Aikaterini Kontou,

Αreti Strati

и другие.

Case Studies in Chemical and Environmental Engineering, Год журнала: 2022, Номер 6, С. 100224 - 100224

Опубликована: Июнь 23, 2022

The ongoing pandemic caused by the emergence of SARS-CoV-2 has resulted in millions deaths worldwide despite various measures announced authorities. Wastewater-based epidemiology ability to provide a day-to-day estimation number infected people fast and cost-effective manner. However, owing complex nature wastewater, wastewater monitoring for viral genome copies is affected extensive fragmentation that takes place all way sewage analytical lab. aim this study was evaluate different methodologies concentration extraction viruses wastewaters select improve an option maximizes recovery SARS-CoV-2. We compare 5 methods 4 commercially available kits RNA extraction. To performance these, isolated from patients used as spike control. Additionally, presence samples determined using reverse transcription quantitative PCR (RT-qPCR) droplet digital (RT-ddPCR), targeting three genetic markers (N1, N2 N3). Using spiked samples, recoveries were estimated 2.1-37.6% 0.1-2.1% kits. It found direct capture-based method, evaluated against variety methods, best terms recovery, time cost. Interestingly, we noticed good agreement between results provided RT-qPCR RT-ddPCR recovery. This evaluation can serve guide laboratories establishing protocol perform Overall, data presented here reinforces validity WBE surveillance, uncovers potential caveats selection protocols points towards optimal solutions maximize its potential.

Язык: Английский

Процитировано

22
Accumulation of SARS-CoV-2 RNA in Sewer Biofilms DOI
William R. Morales Medina, Stephanie D’Elia, Nicole Fahrenfeld

и другие.

ACS ES&T Water, Год журнала: 2022, Номер 2(11), С. 1844 - 1851

Опубликована: Янв. 21, 2022

Wastewater-based epidemiology is a useful tool to track COVID-19 community infections. While recommendations exist for normalizing wastewater virus concentrations the flow and number of people in sewershed, less information available regarding potential in-sewer processes impact these observations. The aim this research was evaluate SARS-CoV-2 accumulation simulated sewer biofilms. An annular biofilm reactor continuously fed with raw from separate sanitary during periods high low incidence. Periodic samples were collected, pepper mottle gene copies quantified via RT-qPCR. During period incidence, below detection or quantitation limit increased across 4-week study period. Accumulation ∼700 genome copies/cm2 observed correlated cumulative cases county. both periods, plateaued after 1 week. These results provide insight into retardation biofilms, which may be interest interpreting wastewater-based data, protecting utility workers, monitoring

Язык: Английский

Процитировано

20
Longer amplicons provide better sensitivity for electrochemical sensing of viral nucleic acid in water samples using PCB electrodes DOI Creative Commons

Shruti Ahuja,

Manish Kumar, Ruchira Nandeshwar

и другие.

Scientific Reports, Год журнала: 2022, Номер 12(1)

Опубликована: Май 25, 2022

Abstract The importance of monitoring environmental samples has gained a lot prominence since the onset COVID-19 pandemic, and several surveillance efforts are underway using gold standard, albeit expensive qPCR-based techniques. Electrochemical DNA biosensors could offer potential cost-effective solution suitable for water in lower middle income countries. In this work, we demonstrate electrochemical detection amplicons as long $${503}\,\hbox {bp}$$ 503 bp obtained from Phi6 bacteriophage (a popular surrogate SARS-CoV-2) isolated spiked lake samples, ENIG finish PCB electrodes with no surface modification. sensor response is thoroughly characterised two fragments different lengths ( $${117}\,\hbox 117 ), impact salt PCR master mix on methylene blue (MB)-DNA interactions studied. Our findings establish that length fragment significantly determines sensitivity, ability to detect without gel purification products demonstrated work bodes well realisation fully-automated solutions situ measurement viral load samples.

Язык: Английский

Процитировано

20
SARS-CoV-2 concentration in wastewater consistently predicts trends in COVID-19 case counts by at least two days across multiple WWTP scales DOI Creative Commons
Candice L. Swift, Mirza Isanovic, Karlen E. Correa Velez

и другие.

Environmental Advances, Год журнала: 2023, Номер 11, С. 100347 - 100347

Опубликована: Янв. 25, 2023

Wastewater surveillance of SARS-CoV-2 has proven instrumental in mitigating the spread COVID-19 by providing an economical and equitable approach to disease surveillance. Here, we analyze correlation RNA influents seven wastewater plants (WWTPs) across state South Carolina with corresponding daily case counts determine whether underlying characteristics WWTPs sewershed populations predict stronger correlations. The served these have varying social vulnerability represent 24% population. study spanned 15 months from April 19, 2020, July 1, 2021, which includes administration first vaccines. concentrations were measured either reverse transcription quantitative PCR (RT-qPCR) or droplet digital (RT-ddPCR). Although average flow rate varied WWTPs, strongest was identified for six when lagged two days after concentration wastewater. weakest found WWTP 6, had lowest ratio population rate, indicating that signal too dilute a robust correlation. Smoothing 7-day moving improved strength between while dampening effect lag-time optimization. Correlation cases compared determined at ZIP-code levels. correlations using ZIP-code-level versus sewershed-level not statistically different WWTPs. Results indicate surveillance, even without normalization fecal indicators, is strong predictor clinical least days, especially RT-ddPCR. Furthermore, may be useful metric assess suitable program.

Язык: Английский

Процитировано

13