Evaluation of viral concentration and extraction methods for SARS-CoV-2 recovery from wastewater using droplet digital and quantitative RT-PCR DOI Creative Commons

Lampros Dimitrakopoulos,

Aikaterini Kontou,

Αreti Strati

и другие.

Case Studies in Chemical and Environmental Engineering, Год журнала: 2022, Номер 6, С. 100224 - 100224

Опубликована: Июнь 23, 2022

The ongoing pandemic caused by the emergence of SARS-CoV-2 has resulted in millions deaths worldwide despite various measures announced authorities. Wastewater-based epidemiology ability to provide a day-to-day estimation number infected people fast and cost-effective manner. However, owing complex nature wastewater, wastewater monitoring for viral genome copies is affected extensive fragmentation that takes place all way sewage analytical lab. aim this study was evaluate different methodologies concentration extraction viruses wastewaters select improve an option maximizes recovery SARS-CoV-2. We compare 5 methods 4 commercially available kits RNA extraction. To performance these, isolated from patients used as spike control. Additionally, presence samples determined using reverse transcription quantitative PCR (RT-qPCR) droplet digital (RT-ddPCR), targeting three genetic markers (N1, N2 N3). Using spiked samples, recoveries were estimated 2.1-37.6% 0.1-2.1% kits. It found direct capture-based method, evaluated against variety methods, best terms recovery, time cost. Interestingly, we noticed good agreement between results provided RT-qPCR RT-ddPCR recovery. This evaluation can serve guide laboratories establishing protocol perform Overall, data presented here reinforces validity WBE surveillance, uncovers potential caveats selection protocols points towards optimal solutions maximize its potential.

Язык: Английский

SARS-CoV-2 wastewater monitoring in rural and small metropolitan communities in Central Michigan DOI Open Access
Michael J. Conway,

Stephanie Kado,

Breanna K. Kooienga

и другие.

The Science of The Total Environment, Год журнала: 2023, Номер 894, С. 165013 - 165013

Опубликована: Июнь 22, 2023

Язык: Английский

Процитировано

14

The role of wastewater-based epidemiology for SARS-CoV-2 in developing countries: Cumulative evidence from South Africa supports sentinel site surveillance to guide public health decision-making DOI
Chinwe Juliana Iwu, Nkosenhle Ndlovu, R. Saïd

и другие.

The Science of The Total Environment, Год журнала: 2023, Номер 903, С. 165817 - 165817

Опубликована: Июль 26, 2023

Язык: Английский

Процитировано

14

Effect of wastewater collection and concentration methods on assessment of viral diversity DOI
Yabing Li,

Brijen Miyani,

Kevin L. Childs

и другие.

The Science of The Total Environment, Год журнала: 2023, Номер 908, С. 168128 - 168128

Опубликована: Ноя. 1, 2023

Язык: Английский

Процитировано

14

Wastewater-based epidemiology for COVID-19 surveillance and beyond: A survey DOI Creative Commons
Chen Chen,

Yunfan Wang,

Gursharn Kaur

и другие.

Epidemics, Год журнала: 2024, Номер 49, С. 100793 - 100793

Опубликована: Сен. 26, 2024

Язык: Английский

Процитировано

6

Evaluation of viral concentration and extraction methods for SARS-CoV-2 recovery from wastewater using droplet digital and quantitative RT-PCR DOI Creative Commons

Lampros Dimitrakopoulos,

Aikaterini Kontou,

Αreti Strati

и другие.

Case Studies in Chemical and Environmental Engineering, Год журнала: 2022, Номер 6, С. 100224 - 100224

Опубликована: Июнь 23, 2022

The ongoing pandemic caused by the emergence of SARS-CoV-2 has resulted in millions deaths worldwide despite various measures announced authorities. Wastewater-based epidemiology ability to provide a day-to-day estimation number infected people fast and cost-effective manner. However, owing complex nature wastewater, wastewater monitoring for viral genome copies is affected extensive fragmentation that takes place all way sewage analytical lab. aim this study was evaluate different methodologies concentration extraction viruses wastewaters select improve an option maximizes recovery SARS-CoV-2. We compare 5 methods 4 commercially available kits RNA extraction. To performance these, isolated from patients used as spike control. Additionally, presence samples determined using reverse transcription quantitative PCR (RT-qPCR) droplet digital (RT-ddPCR), targeting three genetic markers (N1, N2 N3). Using spiked samples, recoveries were estimated 2.1-37.6% 0.1-2.1% kits. It found direct capture-based method, evaluated against variety methods, best terms recovery, time cost. Interestingly, we noticed good agreement between results provided RT-qPCR RT-ddPCR recovery. This evaluation can serve guide laboratories establishing protocol perform Overall, data presented here reinforces validity WBE surveillance, uncovers potential caveats selection protocols points towards optimal solutions maximize its potential.

Язык: Английский

Процитировано

23