Case Studies in Chemical and Environmental Engineering, Год журнала: 2022, Номер 6, С. 100224 - 100224

Опубликована: Июнь 23, 2022

The ongoing pandemic caused by the emergence of SARS-CoV-2 has resulted in millions deaths worldwide despite various measures announced authorities. Wastewater-based epidemiology ability to provide a day-to-day estimation number infected people fast and cost-effective manner. However, owing complex nature wastewater, wastewater monitoring for viral genome copies is affected extensive fragmentation that takes place all way sewage analytical lab. aim this study was evaluate different methodologies concentration extraction viruses wastewaters select improve an option maximizes recovery SARS-CoV-2. We compare 5 methods 4 commercially available kits RNA extraction. To performance these, isolated from patients used as spike control. Additionally, presence samples determined using reverse transcription quantitative PCR (RT-qPCR) droplet digital (RT-ddPCR), targeting three genetic markers (N1, N2 N3). Using spiked samples, recoveries were estimated 2.1-37.6% 0.1-2.1% kits. It found direct capture-based method, evaluated against variety methods, best terms recovery, time cost. Interestingly, we noticed good agreement between results provided RT-qPCR RT-ddPCR recovery. This evaluation can serve guide laboratories establishing protocol perform Overall, data presented here reinforces validity WBE surveillance, uncovers potential caveats selection protocols points towards optimal solutions maximize its potential.

Язык: Английский