Abstract
Background
Cellulose
is
the
major
part
of
lignocellulosic
biomass.
It
can
be
hydrolyzed
into
glucose
units
via
specific
enzymes
called
cellulases
that
have
been
applied
in
many
commercial
fields.
There
are
several
studies
illustrate
influence
on
apple
juice
clarification.
However,
to
best
our
knowledge,
effect
microbial
cellulase
volatile
compounds
not
well
known.
The
present
study
aimed
assess
from
a
new
bacterial
isolate
physicochemical
properties
as
compounds.
hydrolysis
some
polysaccharides
(cellulose,
hemicellulose,
pectin)
and
polyphenols
during
production
necessary
reduce
cloud
sedimentation
or
color
deterioration
increase
yield
juice.
So,
isolates
serve
processing
aids
obtain
clear
with
high
yield.
Results
Cellulase-producing
bacterium
was
isolated,
characterized
molecularly
identified
Bacillus
licheniformis
strain-MA1
an
accession
number
ON840115.
Optimization
medium
parameters
implemented
using
Plackett–Burman
design
(PBd)
followed
by
Box-Behnken
(BBd)
response
surface
methodology
(RSM).
PBd
revealed
three
most
important
(significant)
variables
including
carboxymethyl
cellulose
(CMC),
corn
cob,
peptone
had
positive
impact
production.
Additionally,
agricultural
residue
(corn
cob)
strain
carbon
source
helps
reducing
costs
enzyme
production,
recycling
by-products,
preserving
environment.
optimized
BBd
enhanced
B.
6.8-fold.
A
remarkable
observed
treated-juice
sample
(88.2
±
0.15%)
comparison
control
(75.4
0.09%).
total
phenolic
contents
cloudy
clarified
juices
were
0.957
0.09
0.412
0.03
mg/mL,
respectively.
Also,
DPPH
FRAP
assays
showed
antioxidant
activity
(Low
IC
50
)
compared
treatment.
Twenty-seven
extracted
headspace
solid-phase
microextraction-gas
analysis
performed
GC–MS.
constituents
belonged
chemical
classes:
15
esters;
6
alcohols;
4
aldehydes
2
acids.
predominant
class
fraction
esters
sweet
fruity
odor.
Conclusion
crude
obtained
novel
successfully
clarifying
agent
Journal of Fungi,
Год журнала:
2023,
Номер
9(11), С. 1069 - 1069
Опубликована: Ноя. 1, 2023
Textile
dyes
are
one
of
the
major
water
pollutants
released
into
in
various
ways,
posing
serious
hazards
for
both
aquatic
organisms
and
human
beings.
Bioremediation
is
a
significantly
promising
technique
dye
decolorization.
In
present
study,
fungal
strain
Lasiodiplodia
sp.
was
isolated
from
fruiting
bodies
Schizophyllum
first
time.
The
examined
laccase
enzyme
production
under
solid-state
fermentation
conditions
with
wheat
bran
(WB)
using
ABTS
2,6-Dimethoxyphenol
(DMP)
as
substrates,
then
fermented
(FWB)
evaluated
biosorbent
Congo
red
adsorption
aqueous
solutions
comparison
unfermented
bran.
A
Box–Behnken
design
used
to
optimize
removal
by
FWB
analyze
interaction
effects
between
three
factors:
duration,
pH,
concentration.
Fourier
transform
infrared
spectroscopy
(FTIR),
X-ray
diffraction
(XRD),
scanning
electron
microscopy
(SEM)
were
applied
study
changes
physical
chemical
characteristics
before
after
fermentation.
An
additional
experiment
conducted
investigate
ability
YZH1
remove
dye-containing
liquid
culture.
results
showed
that
produced
throughout
cultivation,
reaching
peak
activities
∼6.2
22.3
U/mL
DMP,
respectively,
on
fourth
day
cultivation.
removed
89.8%
(100
mg
L−1)
solution
12
h
contact,
whereas
WB
only
77.5%.
Based
results,
achieved
93.08%
percentage
6
days
fermentation,
pH
8.5,
150
L−1
concentration
24
h.
95.3%
8
inoculation
These
findings
indicate
this
worthy
candidate
environmental
effluents.
Kuwait Journal of Science,
Год журнала:
2024,
Номер
51(3), С. 100220 - 100220
Опубликована: Март 26, 2024
The
present
study
aims
to
isolate
amylolytic
bacteria
and
later
the
formation
of
a
bacterial
consortium
for
alpha-amylase
production.
potent
strains
in
selected
were
identified
as
B.
subtilis
licheniformis
on
basis
16S
rRNA
sequencing.
Initially
cultural
conditions
alpha
amylase
optimized
Plackett–Burman
design
followed
by
central
composite
design.
ANOVA
optimization
indicated
pH
7,
0.05
%CaCl2
24
h
incubation
time
significant
variables
that
mainly
effect
After
process
statistical
optimization,
enzyme
was
then
partially
purified
means
ammonium
sulfate
precipitation
at
60%
saturation
level,
resulting
2-fold
purification
with
1.4
U/mg
specific
activity.
Enzyme
kinetics
studies
showed
maximum
velocity
reaction
(Vmax)
Michaelis
constant
(Km)
47.4
mg/ml
13.2
mM,
respectively.
Current
results
suggest
newly
isolated
could
be
considered
promising
candidate
industrial
applications.
International Journal of Polymer Science,
Год журнала:
2024,
Номер
2024(1)
Опубликована: Янв. 1, 2024
Senna
didymobotrya
is
one
of
the
emerging
invasive
weeds
in
East
and
South
Africa.
One
solutions
for
weed
management
involves
isolating
cellulose
converting
it
into
valuable
materials.
In
this
research,
isolation
was
optimized
by
Plackett‐Burman
design
(PBD)
response
surface
methodology
using
Box‐Behnken
(BBD),
both
which
are
statistical
empirical
models.
The
maximum
isolated
yield
37.5%
(
w
/
)
sulfuric
acid
1.75%
v
),
sodium
chlorite
3%
at
pH
3.5,
potassium
hydroxide
6%
treatment.
Each
chemical
treatment
done
heating
mixture
90°C
2
hrs.
obtained
(37.5%
))
higher
than
preoptimized
value
35.5%
).
dewaxed
powder
weed,
cellulose,
commercial
characterized
side
comparison
FTIR,
XRD,
SEM,
TGA/DTA.
All
characterizing
methods
indicated
that
material
cellulose.
Even
though
intensity
FTIR
XRD
graphs
crystallinity
index
calculated
from
analysis
showed
(83%)
(78%).
TGA/DTA
indicates
had
comparable
thermal
stability,
stable
up
to
360°C.
Therefore,
method,
S.
opens
avenues
materials
like
carboxymethyl
hydrogel,
nanocrystals.
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Июнь 12, 2024
Abstract
Xenocoumacin
1
(Xcn
1),
antibiotic
discovered
from
secondary
metabolites
of
Xenorhabdus
nematophila
,
had
the
potential
to
develop
into
a
new
pesticide
due
its
excellent
activity
against
bacteria,
oomycetes
and
fungi.
However,
current
low
yield
Xcn1
limits
development
utilization.
To
improve
Xcn1,
response
surface
methodology
was
used
determine
optimal
composition
fermentation
medium
one
factor
at
time
approach
utilized
optimize
process.
The
composed
in
g/L:
proteose
peptone
20.8;
maltose
12.74;
K
2
HPO
4
3.77.
conditions
were
that
25
°C,
initial
pH
7.0,
inoculum
size
10%,
culture
75
mL
250
shake
flask
with
an
agitation
rate
150
rpm
for
48
h.
YL001
produced
highest
(173.99
mg/L)
when
arginine
added
broth
3
mmol/L
12th
Compared
Tryptic
Soy
Broth
medium,
optimized
process
resulted
243.38%
increase
production.
obtained
results
confirmed
optimizing
technology
led
yield.
This
work
would
be
helpful
efficient
production
lay
foundation
industrial
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 4, 2024
Abstract
In
this
study,
biogenesis,
statistical
optimization
and
molecular
modeling
of
alkaline
protease
from
an
estuarine
associated
actinobacterium
Streptomyces
variabilis
was
carried
out
by
Box-Behnken
design.
Initially,
the
biogenesis
selected
attained
through
submerged
condition.
Simultaneously,
actinobacterial
mediated
statistically
optimized
‘one
factor
at
a
time
approach’
using
design
in
basal
medium
constitutes
2.5%
w/v
NaCl
concentration
with
pH
8.0,
temperature
55°C
2.50%
inoculum
size
for
94h
incubation.
The
analysis
variance
(ANOVA)
exhibited
maximum
level
coefficient
(R2
=
0.9720)
more
significance
(P
<
0.0001).
purification
step,
expressed
21.93%
recovery
2.93
fold
last
stage
Sephadex
G-100
chromatography.
Followed
by,
mass
enzyme
calculated
as
35kDa
on
10%
SDS-PAGE.
three
dimensional
structure
purified
predicted
encoded
total
amino
acid
content
481.
stability
range
denoted
8,
60°C
fermentation
constituted
1mM
Mg2+,
3.5%
casein.
kinetic
parameters
like
KmVmax
showed
5.158mg/ml
484.90
±
2.04µg/min/mg,
respectively.
Further,
degradation
efficacy
interaction
between
well
dye
molecules
acridine
orange
erythrosine
pink
were
assessed
in-silico
docking
method
online
Swiss
software
tool.
decolouration
dyes
evaluated
first
order
study
R2
values
0.9987
&
0.9953
By
keeping
view,
could
be
validated
that
is
potent
strain
production
also
used
decoulouring
agent.
