Application of statistical designs strategy to improve cellulase production using agro-waste residue by a novel isolate Bacillus licheniformis strain-MA1 and assessing the enzyme effect on apple juice quality DOI Creative Commons
Mohamed A.A. Abdella, Gamil E. Ibrahim

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Nov. 29, 2024

Abstract Background Cellulose is the major part of lignocellulosic biomass. It can be hydrolyzed into glucose units via specific enzymes called cellulases that have been applied in many commercial fields. There are several studies illustrate influence on apple juice clarification. However, to best our knowledge, effect microbial cellulase volatile compounds not well known. The present study aimed assess from a new bacterial isolate physicochemical properties as compounds. hydrolysis some polysaccharides (cellulose, hemicellulose, pectin) and polyphenols during production necessary reduce cloud sedimentation or color deterioration increase yield juice. So, isolates serve processing aids obtain clear with high yield. Results Cellulase-producing bacterium was isolated, characterized molecularly identified Bacillus licheniformis strain-MA1 an accession number ON840115. Optimization medium parameters implemented using Plackett–Burman design (PBd) followed by Box-Behnken (BBd) response surface methodology (RSM). PBd revealed three most important (significant) variables including carboxymethyl cellulose (CMC), corn cob, peptone had positive impact production. Additionally, agricultural residue (corn cob) strain carbon source helps reducing costs enzyme production, recycling by-products, preserving environment. optimized BBd enhanced B. 6.8-fold. A remarkable observed treated-juice sample (88.2 ± 0.15%) comparison control (75.4 0.09%). total phenolic contents cloudy clarified juices were 0.957 0.09 0.412 0.03 mg/mL, respectively. Also, DPPH FRAP assays showed antioxidant activity (Low IC 50 ) compared treatment. Twenty-seven extracted headspace solid-phase microextraction-gas analysis performed GC–MS. constituents belonged chemical classes: 15 esters; 6 alcohols; 4 aldehydes 2 acids. predominant class fraction esters sweet fruity odor. Conclusion crude obtained novel successfully clarifying agent

Language: Английский

Valorization of chicken feather waste using recombinant bacillus subtilis cells by solid-state fermentation for soluble proteins and serine alkaline protease production DOI

Dina Hassan El Salamony,

Mohamed Salah El-Din Hassouna, Taha I. Zaghloul

et al.

Bioresource Technology, Journal Year: 2023, Volume and Issue: 393, P. 130110 - 130110

Published: Nov. 29, 2023

Language: Английский

Citations

16
Genetic Modification of Aurantiochytrium sp. 18W-13a for Enhancement of Proteolytic Activity by Heterologous Expression of Extracellular Proteases DOI
Kohei Yoneda, Chun Hung Man, Yoshiaki Maeda

et al.

Marine Biotechnology, Journal Year: 2024, Volume and Issue: 26(5), P. 853 - 861

Published: Jan. 5, 2024

Language: Английский

Citations

3
Decolorization of Textile Azo Dye via Solid-State Fermented Wheat Bran by Lasiodiplodia sp. YZH1 DOI Creative Commons
Ali Borham,

Mohammad K. Okla,

Mohamed A. El‐Tayeb

et al.

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(11), P. 1069 - 1069

Published: Nov. 1, 2023

Textile dyes are one of the major water pollutants released into in various ways, posing serious hazards for both aquatic organisms and human beings. Bioremediation is a significantly promising technique dye decolorization. In present study, fungal strain Lasiodiplodia sp. was isolated from fruiting bodies Schizophyllum first time. The examined laccase enzyme production under solid-state fermentation conditions with wheat bran (WB) using ABTS 2,6-Dimethoxyphenol (DMP) as substrates, then fermented (FWB) evaluated biosorbent Congo red adsorption aqueous solutions comparison unfermented bran. A Box–Behnken design used to optimize removal by FWB analyze interaction effects between three factors: duration, pH, concentration. Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM) were applied study changes physical chemical characteristics before after fermentation. An additional experiment conducted investigate ability YZH1 remove dye-containing liquid culture. results showed that produced throughout cultivation, reaching peak activities ∼6.2 22.3 U/mL DMP, respectively, on fourth day cultivation. removed 89.8% (100 mg L−1) solution 12 h contact, whereas WB only 77.5%. Based results, achieved 93.08% percentage 6 days fermentation, pH 8.5, 150 L−1 concentration 24 h. 95.3% 8 inoculation These findings indicate this worthy candidate environmental effluents.

Language: Английский

Citations

4
Response surface methodology as an approach for optimization of alpha amylase production by using bacterial consortium under submerged fermentation DOI Creative Commons
Roheena Abdullah,

Sitara Ahmad,

Kinza Nisar

et al.

Kuwait Journal of Science, Journal Year: 2024, Volume and Issue: 51(3), P. 100220 - 100220

Published: March 26, 2024

The present study aims to isolate amylolytic bacteria and later the formation of a bacterial consortium for alpha-amylase production. potent strains in selected were identified as B. subtilis licheniformis on basis 16S rRNA sequencing. Initially cultural conditions alpha amylase optimized Plackett–Burman design followed by central composite design. ANOVA optimization indicated pH 7, 0.05 %CaCl2 24 h incubation time significant variables that mainly effect After process statistical optimization, enzyme was then partially purified means ammonium sulfate precipitation at 60% saturation level, resulting 2-fold purification with 1.4 U/mg specific activity. Enzyme kinetics studies showed maximum velocity reaction (Vmax) Michaelis constant (Km) 47.4 mg/ml 13.2 mM, respectively. Current results suggest newly isolated could be considered promising candidate industrial applications.

Language: Английский

Citations

1
Immobilization of alkaline protease produced by Streptomyces rochei strain NAM-19 in solid state fermentation based on medium optimization using central composite design DOI

Asmaa I. El-Shazly,

Marwa I. Wahba,

Nayera A. M. Abdelwahed

et al.

3 Biotech, Journal Year: 2024, Volume and Issue: 14(6)

Published: May 22, 2024

Language: Английский

Citations

1
A Statistical Empirical Model and RSM‐Guided Isolation and Characterization of Cellulose from Invasive Weed Senna didymobotrya (Fresen.) Irwin & Barneby, through Chemical and Spectroscopic Techniques DOI Creative Commons
Meseret Zebeaman Birhanu, Mesfin Getachew Tadesse, Rakesh Kumar Bachheti

et al.

International Journal of Polymer Science, Journal Year: 2024, Volume and Issue: 2024(1)

Published: Jan. 1, 2024

Senna didymobotrya is one of the emerging invasive weeds in East and South Africa. One solutions for weed management involves isolating cellulose converting it into valuable materials. In this research, isolation was optimized by Plackett‐Burman design (PBD) response surface methodology using Box‐Behnken (BBD), both which are statistical empirical models. The maximum isolated yield 37.5% ( w / ) sulfuric acid 1.75% v ), sodium chlorite 3% at pH 3.5, potassium hydroxide 6% treatment. Each chemical treatment done heating mixture 90°C 2 hrs. obtained (37.5% )) higher than preoptimized value 35.5% ). dewaxed powder weed, cellulose, commercial characterized side comparison FTIR, XRD, SEM, TGA/DTA. All characterizing methods indicated that material cellulose. Even though intensity FTIR XRD graphs crystallinity index calculated from analysis showed (83%) (78%). TGA/DTA indicates had comparable thermal stability, stable up to 360°C. Therefore, method, S. opens avenues materials like carboxymethyl hydrogel, nanocrystals.

Language: Английский

Citations

1
Optimization and purification of a novel calcium-independent thermostable, α-amylase produced by Bacillus licheniformis UDS-5 DOI
Sadikhusain Suthar, Disha Joshi, Harsh Patel

et al.

World Journal of Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 40(12)

Published: Nov. 19, 2024

Language: Английский

Citations

1
Wheat bran submerged fermentation for protease production by a newly Streptomyces sp. isolate: optimization by factorial designs DOI
Habiba Zerizer, Faiza Boughachiche, A. Ait Kaki

et al.

Biomass Conversion and Biorefinery, Journal Year: 2024, Volume and Issue: 14(12), P. 13503 - 13512

Published: April 26, 2024

Language: Английский

Citations

0
Enhancing the yield of Xenocoumacin 1 in Xenorhabdus nematophila YL001 by optimizing the fermentation process DOI Creative Commons
Yunfei Han, Shujing Zhang, Yang Wang

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: June 12, 2024

Abstract Xenocoumacin 1 (Xcn 1), antibiotic discovered from secondary metabolites of Xenorhabdus nematophila , had the potential to develop into a new pesticide due its excellent activity against bacteria, oomycetes and fungi. However, current low yield Xcn1 limits development utilization. To improve Xcn1, response surface methodology was used determine optimal composition fermentation medium one factor at time approach utilized optimize process. The composed in g/L: proteose peptone 20.8; maltose 12.74; K 2 HPO 4 3.77. conditions were that 25 °C, initial pH 7.0, inoculum size 10%, culture 75 mL 250 shake flask with an agitation rate 150 rpm for 48 h. YL001 produced highest (173.99 mg/L) when arginine added broth 3 mmol/L 12th Compared Tryptic Soy Broth medium, optimized process resulted 243.38% increase production. obtained results confirmed optimizing technology led yield. This work would be helpful efficient production lay foundation industrial

Language: Английский

Citations

0
Biogenesis, molecular characterization and dye degradation efficacy of alkaline protease from an estuarine associated actinobacterium Streptomyces variabilis using in-silico method DOI Creative Commons

P. Balamurugan,

Silambarasan Tamil Selvan

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 4, 2024

Abstract In this study, biogenesis, statistical optimization and molecular modeling of alkaline protease from an estuarine associated actinobacterium Streptomyces variabilis was carried out by Box-Behnken design. Initially, the biogenesis selected attained through submerged condition. Simultaneously, actinobacterial mediated statistically optimized ‘one factor at a time approach’ using design in basal medium constitutes 2.5% w/v NaCl concentration with pH 8.0, temperature 55°C 2.50% inoculum size for 94h incubation. The analysis variance (ANOVA) exhibited maximum level coefficient (R2 = 0.9720) more significance (P < 0.0001). purification step, expressed 21.93% recovery 2.93 fold last stage Sephadex G-100 chromatography. Followed by, mass enzyme calculated as 35kDa on 10% SDS-PAGE. three dimensional structure purified predicted encoded total amino acid content 481. stability range denoted 8, 60°C fermentation constituted 1mM Mg2+, 3.5% casein. kinetic parameters like Km Vmax showed 5.158mg/ml 484.90 ± 2.04µg/min/mg, respectively. Further, degradation efficacy interaction between well dye molecules acridine orange erythrosine pink were assessed in-silico docking method online Swiss software tool. decolouration dyes evaluated first order study R2 values 0.9987 & 0.9953 By keeping view, could be validated that is potent strain production also used decoulouring agent.

Language: Английский

Citations

0