Green Synthesis and Antibacterial Activity of Ag/Fe2O3 Nanocomposite Using Buddleja lindleyana Extract

Bioengineering, Год журнала: 2022, Номер 9(9), С. 452 - 452

Опубликована: Сен. 8, 2022

In the study reported in this manuscript, silver/iron oxide nanocomposites (Ag/Fe2O3) were phytosynthesized using extract of Buddleja lindleyana via a green, economical and eco-friendly strategy. The biosynthesized Ag/Fe2O3 characterized UV-Vis spectrophotometry, FTIR, XRD, TEM, DLS SEM-EDX analyses. particulates showed triangular spherical morphology having sizes between 25 174 nm. FTIR studies on nanoparticles functional groups corresponding to organic metabolites, which reduce stabilize nanocomposite. antimicrobial efficacy against bacterial pathogens was assessed. addition, exhibited broad spectrum activities B. subtilis, S. aureus, E. coli, P. aeruginosa with inhibition zones 23.4 ± 0.75, 22.3 0.57, 20.8 1.6, 19.5 0.5 mm, respectively. composites obtained promising antibacterial action human (S. subtilis aeruginosa), making them candidates for medical applications.

Язык: Английский

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Multifunctional properties of silver and gold nanoparticles synthesis by Fusarium pseudonygamai

Biomass Conversion and Biorefinery, Год журнала: 2022, Номер 14(22), С. 28253 - 28270

Опубликована: Ноя. 11, 2022

Abstract The goal of the current work was to investigate antibacterial, antibiofilm, anticancer, and antioxidant opportunities silver gold nanoparticles (AgNPs AuNPs) synthesized utilizing a new fungus strain called Fusarium pseudonygamai TB-13c. With aid UV, HR-TEM, FTIR, SEM, XRD, NPs’ creation examined. For AgNPs AuNPs, mycosynthesized highest peak plasmon band seen at around 420 540 nm, respectively. ranged in size from 5 20 whereas AuNPs 8 60 nm. were spherical form. AgNPs, angles 38.42°, 44.56°, 64.66°, 77.75°; for 38.56°, 44.74°, 64.87°, 77.85°. antibacterial efficiency evaluated against Klebsiella pneumoniae , Pseudomonas aeruginosa methicillin-sensitive Staphylococcus aureus (MSSA), methicillin-resistant (MRSA). In particular, outperformed their ability combat pathogenic microorganisms. Furthermore, antibiofilm study that shown had activity more than AgNPs. Interestingly, applying DPPH procedure these noble metallic NPs activity, which IC 50 38.2 μg/ mL 180 AuNPs. modification cells evident cytotoxicity evaluation findings as change usual shape, partially or completely loss monolayer, granulation, shrinkage, cell rounding with normal vero 695.34 μg/mL 631.66 respectively, whilst cancer (Mcf7) 204.07 206.95 combined results show there is clear flexible biological use less toxic chemotherapeutic agents.

Язык: Английский

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Cefotaxime incorporated bimetallic silver-selenium nanoparticles: promising antimicrobial synergism, antibiofilm activity, and bacterial membrane leakage reaction mechanism

RSC Advances, Год журнала: 2022, Номер 12(41), С. 26603 - 26619

Опубликована: Янв. 1, 2022

In this research, we reported for the first time simple incorporation of antibiotic cefotaxime (CFM) with Ag NPs, Se and bimetallic Ag–Se NPs by gamma rays; as a promising cost-effective, eco-friendly method.

Язык: Английский

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Influence of biosynthesized magnesium oxide nanoparticles on growth and physiological aspects of cowpea (Vigna unguiculata L.) plant, cowpea beetle, and cytotoxicity

Biotechnology Journal, Год журнала: 2023, Номер 18(12)

Опубликована: Авг. 24, 2023

Recently, agricultural management innovation has incorporated engineered nanoparticles. The current investigation was carried out to produce magnesium oxide nanoparticles (MgONPs) for the first time applying S. cerevisiae extract. FTIR, XRD, HRTEM, and zeta potential analysis were used characterize MgONPs. FTIR data show that bioactive substances reduce cap synthesized crystalline metallic MgONPs had four significant peaks in XRD pattern. size form of validated by TEM, which exhibited spherical structures with an average 27 nm. effect various dosages administered cowpea (Vigna unguiculata L.) plant on all vitro parameters shown be study. concentration 200 ppm most treatment increased shoot length, dry-weight root 27.35%, 45.09%, 31.91% when compared untreated plants. significantly photosynthetic pigments, 150 increasing soluble proteins carbohydrates. effectively treated C. maculatus, dose time-dependent insecticidal activity. death rates varied 82.66% 100% fifth day. Biochemical histopathological studies rats investigated. Rats showed higher GOT, GPT, Urea levels, but lower creatinine, indicating differences. MgONPs-treated rats' liver mild moderate histopathologic changes, including portal blood vessel congestion, lymphocytic cholangitis, degenerative changes. improve development outcomes suppress grain insects (C. maculatus).

Язык: Английский

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Recent Insights into Nanotechnology in Colorectal Cancer

Applied Biochemistry and Biotechnology, Год журнала: 2023, Номер 196(7), С. 4457 - 4471

Опубликована: Сен. 26, 2023

Язык: Английский

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Utilization of biosynthesized silver nanoparticles from Agaricus bisporus extract for food safety application: synthesis, characterization, antimicrobial efficacy, and toxicological assessment

Scientific Reports, Год журнала: 2023, Номер 13(1)

Опубликована: Сен. 12, 2023

The emergence of antimicrobial resistance in foodborne bacterial pathogens has raised significant concerns the food industry. This study explores potential biosynthesized silver nanoparticles (AgNPs) derived from Agaricus bisporus (Mushroom) against pathogens. AgNPs were characterized using various techniques, including UV-visible spectroscopy, X-ray diffraction, Fourier transform infrared high-resolution scanning electron microscopy with energy dispersive dynamic light scattering, and zeta analysis. antibacterial activity was tested a panel strains, their cytotoxicity evaluated on normal human skin fibroblasts. Among Pseudomonas aeruginosa ATCC 27853 showed highest sensitivity an inhibition zone diameter (IZD) 48 mm, while Klebsiella quasipneumoniae ATTC 700603 Bacillus cereus 11778 displayed IZDs 20 mm. cations released by demonstrated strong bactericidal effects both Gram-positive (G + ve) Gram-negative - bacteria, as evidenced minimum inhibitory concentration/minimum concentration (MBC/MIC) ratio. Moreover, testing fibroblasts (HSF) indicated that mushroom extract safe, cell viability 98.2%. Therefore, hold promise alternative means to inhibit biofilm formation industry sector.

Язык: Английский

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Biogenic nanosilver bearing antimicrobial and antibiofilm activities and its potential for application in agriculture and industry

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Фев. 20, 2023

Due to the increasing resistance of bacteria and fungi antimicrobials, it is necessary search for effective alternatives prevent treat pathogens causing diseases in humans, animals, plants. In this context, mycosynthesized silver nanoparticles (AgNPs) are considered as a potential tool combat such pathogenic microorganisms.AgNPs were synthesized from Fusarium culmorum strain JTW1 characterized by Transmission Electron Microscopy (TEM), X-ray diffraction (XRD), Fourier Transform Infrared (FTIR) spectroscopy, Nanoparticle Tracking Analysis (NTA), Dynamic Light Scattering (DLS) Zeta measurement. The minimum inhibitory (MIC) biocidal concentrations (MBC) determined against 13 bacterial strains. Moreover, combined effect AgNPs with antibiotics (streptomycin, kanamycin, ampicillin, tetracycline) was also studied determining Fractional Inhibitory Concentration (FIC) index. anti-biofilm activity examined crystal violet fluorescein diacetate (FDA) assays. Furthermore, antifungal evaluated panel phytopathogenic viz., Botrytis, Colletotrichum, Fusarium, Phoma, Sclerotinia, an oomycete pathogen Phytophthora agar well-diffusion micro-broth dilution method evaluate minimal that inhibit fungal spore germination.Fungi-mediated synthesis resulted formation small (15.56 ± 9.22 nm), spherical stable (zeta - 38.43 mV) good crystallinity. results FTIR spectroscopy indicated presence various functional groups, namely hydroxyl, amino, carboxyl ones, biomolecules on surface AgNPs. showed antimicrobial antibiofilm activities Gram-positive Gram-negative bacteria. values MIC MBC ranged between 16-64 32-512 μg mL-1, respectively. enhanced combination confirmed human pathogens. highest synergistic (FIC = 0.0625) demonstrated streptomycin two strains Escherichia coli (ATCC 25922 ATCC 8739), followed Klebsiella pneumoniae Pseudomonas aeruginosa 0.125). Enhanced effects ampicillin shown Staphylococcus aureus 25923 0.125) P. 0.25), well kanamycin S. 6538 0.25). assay revealed lowest concentration (0.125 mL-1) reduced development biofilms Listeria monocytogenes Salmonella enterica, while maximum infantis, its biofilm after exposure 512 mL-1. A high hydrolases observed FDA assay. at 0.125 mL-1 hydrolytic all formed tested pathogens, except E. 25922, aeruginosa, Pectobacterium carotovorum (efficient 2-fold higher, 0.25 mL-1), 8739, infantis suppressed treatment 0.5, 2 8 inhibited growth germination Botrytis cinerea, Phoma lingam, Sclerotinia sclerotiorum. MFC spores these 64, 256, 32 zones inhibition 4.93, 9.54, 3.41 mm, respectively.Fusarium found be eco-friendly biological system easy, efficient inexpensive our study, mycosynthesised remarkable (antibacterial antifungal) wide range plant singly antibiotics. These could applied medicine, agriculture, food industry control cause numerous crop losses. However, before using them extensive animal studies required toxicity, if any.

Язык: Английский

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A Mini-Review of Synthetic Organic and Nanoparticle Antimicrobial Agents for Coatings in Textile Applications

Coatings, Год журнала: 2023, Номер 13(4), С. 693 - 693

Опубликована: Март 28, 2023

Many synthetic compounds have been applied to impart antimicrobial properties fabrics. In this review, the types of bacteria are described. Furthermore, agents, namely quaternary ammonium (QACs), polyhexamethylene biguanide (PHMB), triclosan, and nitrogen-halamines (N-halamines), discussed along with their properties, advantages disadvantages. Although agents neutralise microorganisms, some adversely affect environment, safety health. These problems led a novel generation coating treatments on textiles, such as copper nanoparticles (CNPs) silver (AgNPs) formed plant extracts, chitosan green synthesis, lower environmental impact but unaltered premium performance improved durability.

Язык: Английский

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Biogenic Nanomaterials: Synthesis, Characterization, and Applications

Environmental science and engineering, Год журнала: 2024, Номер unknown, С. 13 - 43

Опубликована: Янв. 1, 2024

Язык: Английский

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Hindering the biofilm of microbial pathogens and cancer cell lines development using silver nanoparticles synthesized by epidermal mucus proteins from Clarias gariepinus

BMC Biotechnology, Год журнала: 2024, Номер 24(1)

Опубликована: Май 4, 2024

Abstract Scientists know very little about the mechanisms underlying fish skin mucus, despite fact that it is a component of immune system. Fish mucus an important defence against invasive infections. Recently, and its are gaining interest among immunologists. Characterization was done on obtained silver nanoparticles Ag combined with Clarias gariepinus catfish epidermal proteins (EMP-Ag-NPs) through UV–vis, FTIR, XRD, TEM, SEM. Ag-NPs ranged in size from 4 to 20 nm, spherical form angles were 38.10°, 44.20°, 64.40°, 77.20°, Where wavelength change after formation EMP-Ag-NPs as indicate dark brown, broad band recorded at 391 nm. Additionally, antimicrobial, antibiofilm anticancer activities assessed. The present results demonstrate high activity unicellular fungi C. albicans , followed by E. faecalis . Antibiofilm showed strong both S. aureus P. aeruginosa pathogens dose-dependent manner, without affecting planktonic cell growth. Also, cytotoxicity effect investigated normal cells (Vero), breast cancer (Mcf7) hepatic carcinoma (HepG2) lines concentrations (200–6.25 µg/mL) current highly 100, 5 25 µg/mL exhibited rounding, shrinkage, deformation granulation Mcf7 HepG2 IC50 19.34 31.16 respectively while Vero appeared rounded concentration 50 shape 25, 12.5 6.25 µg/ml 35.85 µg/mL. This study evidence potential efficacy biologically generated substitute medicinal agent harmful microorganisms. Furthermore, highlights their inhibitory lines.

Язык: Английский

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