Ecology and Evolution,
Год журнала:
2024,
Номер
14(11)
Опубликована: Ноя. 1, 2024
ABSTRACT
Incidental
mortality
in
fisheries
is
a
major
driver
of
population
declines
for
albatrosses
and
petrels
globally.
However,
accurate
identification
species
can
be
difficult
due
to
the
poor
condition
bycaught
birds
and/or
visual
similarities
between
closely
related
species.
We
assessed
three
genetic
markers
their
ability
distinguish
36
albatross
petrel
listed
Annex
1
Agreement
on
Conservation
Albatrosses
Petrels
(ACAP)
Australia's
Threat
Abatement
Plan
(TAP)
bycatch
seabirds
during
oceanic
longline
fishing
operations.
generated
275
new
sequences,
from
29
species,
improve
coverage
reference
databases
these
The
combined
use
selected
Cytochrome
b
Control
Region
enabled
31
level
four
sister
One
could
not
evaluated
as
no
sequences
were
available.
tested
59
feathers
compared
onboard
identification.
successfully
assigned
all
procellariiforms
(
n
=
58),
whereas
only
two
correctly
identified
visually
onboard,
highlighting
difficulty
assignment
need
alternative
methods.
utility
our
chosen
procellariiform
with
74%
or
level.
precautionary
approach
needed
application
beyond
unvalidated
sequences.
described
here
provides
streamlined
framework
molecular
seabird
bycatch.
This
recommended
within
outside
Australian
waters
resolution
reporting
corroborate
logbook
entries,
observer
reports
audits
images
captured
by
electronic
monitoring
systems
well
help
inform
conservation
efforts.
The Science of The Total Environment,
Год журнала:
2024,
Номер
957, С. 177308 - 177308
Опубликована: Ноя. 13, 2024
Lake
Albert,
Africa's
seventh-largest
lake
and
a
biodiversity
hotspot,
faces
significant
environmental
challenges,
including
unregulated
anthropogenic
pressure
lack
of
comprehensive
biological
studies.
To
address
the
scarcity
data,
we
utilized
DNA
(eDNA)
metabarcoding
to
assess
lake's
eukaryotic
metazoan
communities.
Surface
water
samples
were
collected
at
three
distinct
locations:
close
southern
inflow
Semliki
River,
central
part
lake,
northern
Victoria
Nile
outflow
Albert
Nile.
We
aimed
study
ecological
patterns
across
focusing
on
sequence
variant
richness
community
composition,
testing
for
differences
among
locations
between
shoreline
pelagic
zones.
Consistent
with
previous
morphology-based
observations,
our
results
revealed
in
composition
sites,
cyclopoid
copepods
dominating
Distance
from
shore
was
factor
influencing
confirming
expectations
about
effects
nutrient
oxygen
availability
gradients.
However,
reference
data
limited
accurate
taxonomic
assignments.
Despite
these
limitations,
demonstrates
that
eDNA
is
highly
useful
assessing
underexplored
tropical
freshwater
ecosystems.
advocate
urgent
efforts
generate
sequences
regions
enhance
utility
monitoring
conservation.
Our
findings
underscore
potential
providing
insights
into
entire
communities
emphasize
need
studies
addressing
full
spectrum
Mathematical Biology and Bioinformatics,
Год журнала:
2024,
Номер
19(2), С. 369 - 392
Опубликована: Ноя. 14, 2024
In
this
paper,
we
investigate
the
possibility
of
using
multiplex
virtual
PCR
method
to
develop
approaches
barcoding
genomes
T4-type
bacteriophages.
A
comparison
and
phylogenomic
analysis
based
on
alignment-free
approach
was
performed.
dataset
Tequatrovirus
previously
selected
for
pangenomic
used
study.
The
were
preliminarily
checked
their
adequate
annotation
and,
accordingly,
sequencing
accuracy.
study
carried
out
67
genomes,
barcodes
generated,
similarity
parameters
determined.
Based
obtained
data,
a
phylogenetic
tree
constructed
neighbor
joining
method.
For
same
set
Straboviridae
bacteriophage
pairwise
distance
matriх
between
oligonucleotide
10-mer
sets.
Comparison
these
two
trees
showed
differences
in
branching
control
distantly
related
from
genera
Mosigvirus
Dhakavirus,
branch
bacteriophages
genus
Tequatrovirus.
authors
work
suggested
that
may
reflect
features
method,
which
is
more
applicable
assessing
closely
than
within
with
an
identity
degree
less
75
%.
It
can
be
concluded
allows
one
distinguish
taxonomic
groups
large
bacteriophages,
include
at
level.
Thus,
such
accurately
compare
viruses
length
about
170
kb.
From
practical
point
view,
quite
including
compiled
its
basis
passport
use
practice
phage
therapy.
Ecology and Evolution,
Год журнала:
2024,
Номер
14(11)
Опубликована: Ноя. 1, 2024
ABSTRACT
Incidental
mortality
in
fisheries
is
a
major
driver
of
population
declines
for
albatrosses
and
petrels
globally.
However,
accurate
identification
species
can
be
difficult
due
to
the
poor
condition
bycaught
birds
and/or
visual
similarities
between
closely
related
species.
We
assessed
three
genetic
markers
their
ability
distinguish
36
albatross
petrel
listed
Annex
1
Agreement
on
Conservation
Albatrosses
Petrels
(ACAP)
Australia's
Threat
Abatement
Plan
(TAP)
bycatch
seabirds
during
oceanic
longline
fishing
operations.
generated
275
new
sequences,
from
29
species,
improve
coverage
reference
databases
these
The
combined
use
selected
Cytochrome
b
Control
Region
enabled
31
level
four
sister
One
could
not
evaluated
as
no
sequences
were
available.
tested
59
feathers
compared
onboard
identification.
successfully
assigned
all
procellariiforms
(
n
=
58),
whereas
only
two
correctly
identified
visually
onboard,
highlighting
difficulty
assignment
need
alternative
methods.
utility
our
chosen
procellariiform
with
74%
or
level.
precautionary
approach
needed
application
beyond
unvalidated
sequences.
described
here
provides
streamlined
framework
molecular
seabird
bycatch.
This
recommended
within
outside
Australian
waters
resolution
reporting
corroborate
logbook
entries,
observer
reports
audits
images
captured
by
electronic
monitoring
systems
well
help
inform
conservation
efforts.
