Aquatic
emergent
insect
communities
form
an
important
link
between
aquatic
and
terrestrial
ecosystems,
yet
studying
them
is
costly
time-consuming
as
they
are
usually
diverse
superabundant.
Metabarcoding
a
valuable
tool
to
investigate
arthropod
community
compositions,
however
high-throughput
applications,
such
for
biomonitoring,
require
cost-effective
user-friendly
procedures.
To
if
the
labour-intensive
DNA
purification
step
can
be
omitted
in
metabarcoding,
we
studied
difference
detection
rates
individual
read
abundance
using
standard
versus
direct
PCR
protocols.
with
without
was
performed
artificially
created
of
known
composition
well
on
natural
both
dipteran
family
Chironomidae
compare
rates,
abundances
presence-absence
composition.
We
found
that
here
presented
novel
approach
metabarcoding
did
not
alter
had
minor
effect
communities.
Furthermore,
compositions
chironomid
were
highly
comparable
approaches.
In
conclusion,
showed
protocols
applied
approaches,
possible
application
wider
range
taxa,
enabling
us
study
more
efficiently
future.
Animals,
Journal Year:
2024,
Volume and Issue:
14(23), P. 3396 - 3396
Published: Nov. 25, 2024
Southern
Brazil
is
home
to
a
large
biodiversity
of
elasmobranchs
from
the
Brazilian
coast.
Several
genera
and
species
small
sharks
Triakidae
family
live
in
this
marine
environment.
Studies
on
these
shark
are
scarce,
with
few
genetic
data
little
information
animal
population
structures.
The
present
study
aimed
sequence
complete
mitochondrial
genome
(mtDNA)
endangered
Biomedical and Biotechnology Research Journal (BBRJ),
Journal Year:
2024,
Volume and Issue:
8(4), P. 415 - 421
Published: Oct. 1, 2024
Abstract
Background:
Cinnamomum
parthenoxylon
is
an
important
component
of
tropical
Vietnam
and
has
major
ecological
economic
significance,
which
been
threatened
in
recent
decades
due
to
the
destruction
its
habitat
overexploitation.
Methods:
In
present
study,
first,
three
chloroplast
DNA
(cpDNA)
regions
(
matK,
rbcL
,
trnH-psbA
)
were
initially
examined
identify
C.
species
using
Maximum
Likelihood
(ML).
Then,
genetic
diversity
analysis
Song
Hinh
protection
forest,
Phu
Yen
Province,
Vietnam.
Results:
The
nucleotide
sequences
determined
be
786bp,
513bp,
464bp,
respectively.
novel
have
deposited
Genbank
with
matK
(PQ106670-PQ106689),
(PQ106690-PQ106709),
(PQ1066710-PQ106729).
revealed
that
mean
base
compositions
T
(34.8%,
28.3%,
30.4%),
C
(19.4%,
22.5%,
15.1%),
A
(29.1%,
26.6%,
39.8%),
G
(16.7%,
22.7%,
14.7%),
each
cpDNA
regions,
GC
content
was
discovered
significantly
lower
than
AT
content.
According
ML
phylogenetic
analyses,
all
samples
forest
are
closely
related
GenBank,
robust
supporting
values.
Moreover,
only
0.25%
(2
haplotypes)
showed
polymorphism
region,
whereas
0.78%
(4
region.
most
variable
(3.44%)
(16
intergenic
region
useful
.
variations
moderate,
average
number
differences
(Kt
=
1.042–4.984),
haplotype
(Hd
0.521–0.916),
(Pi
1.33–10.74
×
10–3).
Conclusions:
results
this
study
potential
provide
valuable
information
essential
for
classification,
identification
plant’s
origin,
propagation,
conservation
program
African Entomology,
Journal Year:
2024,
Volume and Issue:
32
Published: Dec. 10, 2024
The
rate
of
human-induced
spread
organisms
is
increasing
with
globalisation.
In
addition,
climate
change
altering
ecosystems,
enabling
species
to
invade
new
environments.
Invertebrates
are
particularly
well-suited
invasion
due
their
generally
small
size
and
short
generation
time
impacts
can
be
extreme.
Therefore,
reliable
identification
a
fundamental
requirement
for
intercepting
such
alien
at
borders
managing
populations,
but
traditional
taxonomic
identifications
time-consuming
often
require
expertise.
DNA
barcoding
molecular
technique
that
rapid,
cost-effective
does
not
this
study,
we
compiled
an
updated
checklist
all
known
invertebrate
in
South
Africa
status
on
the
Barcode
Life
Data
System
(BOLD)
using
previous
published
records
literature.
total,
1013
species,
including
132
biocontrol
agents,
were
found.
Insects,
predominantly
hemipterans,
comprised
most
species.
Overall,
66.8%
dataset
accessioned
BOLD.
However,
few
these
African
specimens
(24.3%).
This
study
marks
first
comprehensive
agent
invertebrates
Africa.
findings
promising
because
many
identified
Molecular
Operational
Taxonomic
Unit
(MOTU)
or
Index
Number
(BIN)
BOLD
COI
barcode.
there
gap
available
barcodes
invertebrates.
As
alters
biogeography
baseline
data
as
will
invaluable
monitoring
limiting
spread.
Aquatic
emergent
insect
communities
form
an
important
link
between
aquatic
and
terrestrial
ecosystems,
yet
studying
them
is
costly
time-consuming
as
they
are
usually
diverse
superabundant.
Metabarcoding
a
valuable
tool
to
investigate
arthropod
community
compositions,
however
high-throughput
applications,
such
for
biomonitoring,
require
cost-effective
user-friendly
procedures.
To
if
the
labour-intensive
DNA
purification
step
can
be
omitted
in
metabarcoding,
we
studied
difference
detection
rates
individual
read
abundance
using
standard
versus
direct
PCR
protocols.
with
without
was
performed
artificially
created
of
known
composition
well
on
natural
both
dipteran
family
Chironomidae
compare
rates,
abundances
presence-absence
composition.
We
found
that
here
presented
novel
approach
metabarcoding
did
not
alter
had
minor
effect
communities.
Furthermore,
compositions
chironomid
were
highly
comparable
approaches.
In
conclusion,
showed
protocols
applied
approaches,
possible
application
wider
range
taxa,
enabling
us
study
more
efficiently
future.
