Study on the effects of Massa Medicata Fermentata with different formulations on the intestinal microbiota and enzyme activities in mice with spleen deficiency constipation

Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 14

Опубликована: Янв. 7, 2025

This study aims to explore the therapeutic mechanism of Massa Medicata Fermentata (MMF) with different formulations on spleen deficiency constipation in mice by analyzing gastrointestinal hormones, D-xylose, intestinal microbiota, and enzyme activities. A model was established using an oral administration Sennae Folium decoction combined controlled diet water intake. After successful establishment, were treated MMF S1, S2, S3. Following intervention, serum samples from each group collected measure VIP, 5-HT, D-xylose. Additionally, small intestine contents analyzed for activity subjected 16S rRNA high-throughput sequencing. Mice showed significant decreases body weight fecal content. In contrast, CS2 CS3 groups returned normal levels, content also normal. The S2 S3 significantly increased protease sucrase enzymes levels compared CM group. Serum D-xylose reduced VIP but decreased groups. 5-HT CS1 significantly, returning showing increases. sequencing analysis revealed that all three effectively restored microbiota composition mice. LEfSe identified characteristic linked intervention upregulated chloroalkane chloroalkene degradation vibrio cholerae pathogenic cycle pathways Candidatus_Arthromitus Psychrobacter positive negative correlations respectively. alleviated symptoms modulating activities, restoring balance. as potential biomarkers treatment constipation. Different have mechanisms regulating through microbiota.

Язык: Английский

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Investigating the biological significance of the TCM principle “promoting urination to regulate bowel movements” through the influence of the intestinal microbiota and their metabolites on the renal-intestinal axis

Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 14

Опубликована: Янв. 10, 2025

Treatment methods in traditional Chinese medicine (TCM) are foundational to their theoretical, methodological, formulaic, and pharmacological systems, significantly contributing syndrome differentiation therapy. The principle of "promoting urination regulate bowel movements" is a common therapeutic approach TCM. core concept the dispersion drainage water dampness, regulating relieve diarrhea," yet its scientific underpinning remains unclear. Modern medical treatment for watery diarrhea primarily focuses on electrolyte replenishment, as diuretics may lead dehydration other side effects. Some reports suggest that this TCM lacks validity. Microecology, an area associated with origins TCM, closely related development, diagnosis, diarrhea. renal-intestinal axis offers molecular biological basis examining pathological mechanisms, advancing targets such "treating intestine address kidney issues" highlighting interactions within "renal-intestinal microbiota-liquid metabolism" framework, thus providing endogenous mechanism support through kidney." An increasing number studies have shown intestinal microbiota metabolites, unique mediators, involved physiological changes body. Therefore, study explores relationship between fluid metabolism from perspective aiming elucidate mechanisms underlying clarify treating via axis. This research provides new insights microbiology.

Язык: Английский

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The intestinal microbiota modulates the visceral sensitivity involved in IBS induced by restraint combined with tail clustering

Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 15

Опубликована: Фев. 20, 2025

To compare three common stimuli that induce emotional stress to identify the optimal method for establishing an animal model aligns with clinical pathogenesis of irritable bowel syndrome (IBS) and explore gut microbiota mechanisms underlying IBS development. Thirty-six SPF-grade female Kunming mice were randomly divided into four groups: normal control (NC) group, restraint (BM) tail clamp (CTM) combined (BCTM) 9 in each group. The NC group was fed normally without any stimulation. BM subjected stress. CTM received intermittent BCTM underwent both stimulation time 1 hour, modeling duration 7 days. General behavioral changes observed. fecal water content measured calculated. pain threshold, gastric residue rate, small intestine propulsion serum levels short-chain fatty acids (SCFAs), serotonin (5-HT), interleukin-10 (IL-10), tumor necrosis factor-alpha (TNF-α) assessed. Histopathological analysis colon tissues performed. 16S rRNA high-throughput sequencing subsequently conducted. effects different on mouse symptoms, gastrointestinal motility, visceral hypersensitivity, inflammation levels, analyzed, correlation Compared BM, CTM, groups presented varying degrees hyperreactivity, accompanied by significantly reduced food intake markedly elevated inflammation, all which are indicative symptoms. Among them, most pronounced hyperreactivity irritability. had higher rates 5-HT a marked reduction tolerance. exhibited dysbiosis, diversity, structural composition, function microbial community. Specific bacterial taxa enriched their corresponding KEGG pathways also altered. Correlation revealed SCFAs positively correlated whereas rate negatively threshold. IL-10 TNF-α, TNF-α. In characteristic bacteria Acinetobacter Akkermansia 5-HT. 1. is superior among protocols successfully induces model. 2. may contribute development induced through regulation

Язык: Английский

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Targeting CD4+ T cells through gut microbiota: therapeutic potential of traditional Chinese medicine in inflammatory bowel disease

Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 15

Опубликована: Март 3, 2025

Inflammatory Bowel Disease (IBD) is an autoimmune disease characterized by chronic relapsing inflammation of the intestinal tract. Gut microbiota (GM) and CD4 + T cells are important in development IBD. A lot studies have shown that GM their metabolites like short-chain fatty acids, bile acids tryptophan can be involved differentiation through various mechanisms, which turn regulate immune homeostasis IBD patients. Therefore, regulating may a potential therapeutic direction for treatment Many Traditional Chinese Medicine (TCM) formulas some herbal extracts affect cell its metabolites. In this review, we mainly focus on role correlation with We also summarize current research progress regulation process TCM.

Язык: Английский

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Massa Medicata Fermentata treated spleen deficiency constipation by mediating intestinal microbiota and serum peptide

Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 15

Опубликована: Март 6, 2025

To investigate the correlation between treatment of spleen deficiency constipation and typical brain intestinal peptides. A total 18 male Kunming mice were randomly divided into three groups (n = 6): normal group (CC), model (CM), Massa Medicata Fermentata intervention (CG). CM CG used to establish a mouse model. After was finished, infused with 0.15 g/mL water infusion at dose 4 g/(kg·day), twice day, 0.4 mL. An equal amount distilled in CC for 7 days. The body weight fecal content monitored during modeling. Following intervention, 16S rRNA amplicon sequencing analyze changes microflora contents, serum substance P (SP), vasoactive peptide (VIP), calcitonin gene-related (CGRP) levels determined via ELISA. modeling had no significant effect on mice, mice's feces greatly reduced, dry hard. Constipation caused by can lead decrease SP an increase VIP CGRP. Fermentata, SP, VIP, CGRP all changed. Intestinal microbiota diversity constipation, dominant characteristic changed, indicating that unbalanced. increased; became Candidatus Arthromitus, Lactobacillus, unclassified Bacilli, Bacillus, Ligilactobacillus, Muribaculaceae, Bacteroides, Enterorhabdus; Arthromitus. Through analysis levels, Ligilactobacillus found be positively correlated negatively Akkermansia Streptococcus Arthromitus CGRP, affect secretion short-chain fatty acids intestine altering microecological environment intestine, then peptides alleviate constipation.

Язык: Английский

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Integrated metabolomics and intestinal microbiota analysis to reveal anti-post-weaning diarrhea mechanisms of Modified Yupingfeng Granule in Rex rabbits

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Апрель 10, 2025

Post-weaning Diarrhea (PWD) is a kind of physiological stress diarrhea in Rex rabbits after weaning, which can lead to death severe cases. Traditional Chinese medicine (TCM) has been widely used animal due its advantages natural origin, diverse functions, safety, reliability, economy and environmental protection. Modified Yupingfeng Granule (MYPFG) an improved prescription based on the famous traditional (YPF), combined with other TCM obvious synergistic additive activity order obtain excellent for PWD. In this study, 120 weaned were randomly allocated 4 treatment groups, including control (CON), low dose (LD), medium (MD), high (HD). Rabbits fed diet or different MYPFG proportions 30 days. The study 16S rRNA analysis intestinal microbiota cecal contents metabolomics explore effect rabbits. increased average daily gain, villus length crypt depth ratio decreased feed meat ratio, frequency, mortality rate, (p < 0.05). test found that could change abundances Patescibacteria, Sphingobium, Ruminococcus, Oxalobacter. Metabolomics may be related regulation Glycine, serine threonine metabolism, Arginine proline metabolism. Nicotinate nicotinamide regulate metabolic pathway some amino acids alleviate PWD

Язык: Английский

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Research progress of traditional Chinese medicine on the treatment of diarrhea by regulating intestinal microbiota and its metabolites based on renal-intestinal axis

Frontiers in Cellular and Infection Microbiology, Год журнала: 2024, Номер 14

Опубликована: Сен. 27, 2024

Intestinal microbiota and its metabolites are involved in many physiological processes of the human body play a vital role maintaining health. The occurrence kidney disease can cause intestinal imbalance, resulting diarrhea. change content aggravate renal function injury, which has bidirectional regulating effect. theory renal-intestinal axis further clarified that impaired is related to imbalance microorganisms, barrier accumulation toxin products. Because unique therapeutic advantages, Traditional Chinese Medicine treat diarrhea by enhancing growth beneficial bacteria, inhibiting pathogenic bacteria immune regulation, slow down continuous deterioration disease. This paper focuses on relationship between diarrhea, influence treatment axis. It provides theoretical basis for regulate based nephrology-induced also new idea method Traitional

Язык: Английский

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Lingguizhugan decoction alleviates obesity in rats on a high-fat diet through the regulation of lipid metabolism and intestinal microbiota

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Ноя. 13, 2024

Background Individuals with obesity often experience elevated blood lipid levels, leading to a chronic low-grade inflammatory state, exacerbating liver oxidative stress, and increasing the risk of various metabolic diseases. Recent evidence suggests that intestinal microbiota short-chain fatty acids (SCFAs) play crucial roles in development progression obesity. While mechanisms by which Lingguizhugan decoction (LGZGD) intervenes improving metabolism, enhancing insulin sensitivity, reducing responses are well-documented, its potential SCFAs remains unclear. This study aims explore impact LGZGD on high-fat diet (HFD) induced rats regulatory effects SCFAs, providing new insights for prevention treatment. Methods Fifty-one male SD were randomly divided into groups, six normal control group (NC) receiving ddH2O treatment standard diet. The remaining 45 fed using D12451 feed. After 10 weeks, HFD gained 20% more weight than NC group, confirming successful modeling These then following groups: model (MC), 20 mg/kg/day Orlistat positive (Orlistat), 1.62 g/kg/day low-dose (LGZGL), 3.24 high-dose (LGZGH) 8 weeks. We evaluated changes body weight, serum total cholesterol (TC), triacylglycerol (TG), low-density lipoprotein (LDL), high-density (HDL) levels. Fat tissues collected pathological analysis. Intestinal contents aseptically 16S rRNA gene sequencing gas chromatography–mass spectrometry (GC–MS) assess gut SCFA Results reduces TC, TG, LDL, HDL significantly hepatic steatosis. Besides, it restored richness diversity microbiota, was reduced HFD, altering overall structure. Specifically, promoted growth Muribaculaceae Dubosiella while inhibiting Christensenellaceae_R_7_group UCG_005 . It also restricts production caproic acid. Correlation analysis indicated correlations: Butyric acid Isovaleric acid; HDL; TC LDL. Conclusion increased abundance beneficial HFD-induced obese rats, improved dysbiosis, inhibited increase content. results suggest can mitigate obesity, active components warrant further investigation.

Язык: Английский

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Elaeagnus angustifolia L. fruit alleviates diarrhea via regulating intestinal microbiota and short chain fatty acids

Heliyon, Год журнала: 2024, Номер 10(19), С. e38646 - e38646

Опубликована: Окт. 1, 2024

Язык: Английский

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External damp environment aggravates diarrhea in spleen deficiency and dampness syndrome in mice: involvement of small intestinal contents microbiota, energy metabolism, gastrointestinal and fluid functions

Frontiers in Cellular and Infection Microbiology, Год журнала: 2024, Номер 14

Опубликована: Окт. 31, 2024

Recent studies have increasingly demonstrated that a multiplatform water environment combined with lard gavage is an effective method for establishing mouse model of diarrhea. However, the interactions between intestinal microorganisms and diarrhea, as well relationships among energy metabolism, fluid balance, gastrointestinal function in this model, remain poorly understood.

Язык: Английский

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