On-chip fabrication of tailored 3D hydrogel scaffolds to model cancer cell invasion and interaction with endothelial cells DOI Creative Commons
Federico Cantoni, Laurent Barbe, Ananya Roy

и другие.

APL Bioengineering, Год журнала: 2024, Номер 8(4)

Опубликована: Дек. 1, 2024

The high mortality associated with certain cancers can be attributed to the invasive nature of tumor cells. Yet, complexity studying invasion hinders our understanding how spreads. This work presents a microengineered three-dimensional (3D) in vitro model for cancer cell and interaction endothelial was generated by printing biomimetic hydrogel scaffold directly on chip using 2-photon polymerization that simulates brain's extracellular matrix. scaffold's geometry specifically designed facilitate growth continuous layer cells one side, while also allowing introduction other side. arrangement confines spatially enables situ microscopy as they invade interact layer. We examined impact 3D parameters hydrogel's physical properties used patient derived glioblastoma study their effect invasion. Notably, tended infiltrate faster when an barrier present. potential adjusting properties, coupled capability real-time observation tumor-endothelial interactions, offers platform tumor–endothelial interactions.

Язык: Английский

Surface Modification Strategies for Biomedical Applications: Enhancing Cell–Biomaterial Interfaces and Biochip Performances DOI

Soonjong Roh,

Yerim Jang,

Jin Yoo

и другие.

BioChip Journal, Год журнала: 2023, Номер 17(2), С. 174 - 191

Опубликована: Июнь 1, 2023

Язык: Английский

Процитировано

45

Microphysiological Systems as Organ-Specific In Vitro Vascular Models for Disease Modeling DOI Creative Commons
Ungsig Nam, Seokhun Lee, Ashfaq Ahmad

и другие.

BioChip Journal, Год журнала: 2024, Номер 18(3), С. 345 - 356

Опубликована: Май 14, 2024

Abstract The vascular system, essential for human physiology, is vital transporting nutrients, oxygen, and waste. Since structures are involved in various disease pathogeneses exhibit different morphologies depending on the organ, researchers have endeavored to develop organ-specific models. While animal models possess sophisticated morphologies, they significant discrepancies from tissues due species differences, which limits their applicability. To overcome limitations arising these oversimplification of 2D dish cultures, microphysiological systems (MPS) emerged as a promising alternative. These more accurately mimic microenvironment by incorporating cell interactions, physical stimuli, extracellular matrix components, thus facilitating enhanced tissue differentiation functionality. Importantly, MPS often utilize human-derived cells, greatly reducing disparities between model patient responses. This review focuses recent advancements MPS, particularly modeling discusses potential biological adaptation.

Язык: Английский

Процитировано

9

Understanding Neovascularization in Glioblastoma: Insights from the Current Literature DOI Open Access
Mariagiovanna Ballato, Emanuela Germanà, Gabriele Ricciardi

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(6), С. 2763 - 2763

Опубликована: Март 19, 2025

Glioblastomas (GBMs), among the most aggressive and resilient brain tumors, characteristically exhibit high angiogenic potential, leading to formation of a dense yet aberrant vasculature, both morphologically functionally. With these premises, numerous expectations were initially placed on anti-angiogenic therapies, soon dashed by their limited efficacy in concretely improving patient outcomes. Neovascularization GBM emerged as complex, dynamic, heterogeneous process, hard manage with classical standard care. Growing evidence has revealed existence non-canonical strategies angiogenesis, variously exploited meet its ever-increasing metabolic demand differently involved tumor progression, recurrence, escape from treatments. In this review, we provide an accurate description each neovascularization mode encountered tumors date, highlighting molecular players signaling cascades primarily involved. We also detail key architectural functional aspects characteristic vascular compartment because intricate crosstalk between different networks. Additionally, explore repertoire emerging therapies against that are currently under study, concluding question: faced such challenging scenario, could combined tailored patient’s genetic signatures, represent effective game changer?

Язык: Английский

Процитировано

1

Biosensor-Enhanced Organ-on-a-Chip Models for Investigating Glioblastoma Tumor Microenvironment Dynamics DOI Creative Commons
Gayathree Thenuwara, Bilal Javed, Baljit Singh

и другие.

Sensors, Год журнала: 2024, Номер 24(9), С. 2865 - 2865

Опубликована: Апрель 30, 2024

Glioblastoma, an aggressive primary brain tumor, poses a significant challenge owing to its dynamic and intricate tumor microenvironment. This review investigates the innovative integration of biosensor-enhanced organ-on-a-chip (OOC) models as novel strategy for in-depth exploration glioblastoma microenvironment dynamics. In recent years, transformative approach incorporating biosensors into OOC platforms has enabled real-time monitoring analysis cellular behaviors within controlled Conventional in vitro vivo exhibit inherent limitations accurately replicating complex nature progression. addresses existing research gap by pioneering models, providing comprehensive platform investigating The applications this combined studying dynamics are critically scrutinized, emphasizing potential bridge between simplistic conditions. Furthermore, article discusses implications elucidating features microenvironment, encompassing cell migration, proliferation, interactions. By furnishing insights, these significantly contribute unraveling biology glioblastoma, thereby influencing development more accurate diagnostic therapeutic strategies.

Язык: Английский

Процитировано

5

Passive-Flow-Based MPS: Emerging Physiological Flow-Mimetic Platforms for Studying Effects of Flow on Single Tissues and Inter-tissue Interactions DOI

Geonho Jin,

Dohyung Kim,

Seonghun Mun

и другие.

BioChip Journal, Год журнала: 2024, Номер 18(2), С. 186 - 210

Опубликована: Март 8, 2024

Язык: Английский

Процитировано

4

3D vascularized microphysiological system for investigation of tumor-endothelial crosstalk in anti-cancer drug resistance DOI
Seunggyu Kim, Joonha Park, Jin‐Nyoung Ho

и другие.

Biofabrication, Год журнала: 2023, Номер 15(4), С. 045016 - 045016

Опубликована: Авг. 11, 2023

Despite the advantages of microfluidic system in drug screening, vascular systems responsible for transport drugs and nutrients have been hardly considered microfluidic-based chemotherapeutic screening. Considering physiological characteristics highly vascularized urinary tumors, we here investigated response bladder tumor cells using a on chip. The chip was designed to open-top region sample introduction hydrophilic rail spontaneous hydrogel patterning, which contributed construction tumor-hydrogel-endothelium interfaces spatiotemporal on-demand manner. Utilizing where intravascularly injected cisplatin diffuse across endothelium into samples, responses cisplatin-resistant or -susceptible were evaluated, showing preservation cellular resistance even within structure also enabled direct harvest samples post analysis terms secretome gene expressions. Comparing efficacy presence absence endothelium, found that proliferation rates increased vasculature-incorporated These suggested our allows establishment vascular-gel-tumor manners further enables investigations

Язык: Английский

Процитировано

9

Imaging‐Based Efficacy Evaluation of Cancer Immunotherapy in Engineered Tumor Platforms and Tumor Organoids DOI Creative Commons
Seong‐Eun Kim,

Suji Yun,

Junsang Doh

и другие.

Advanced Healthcare Materials, Год журнала: 2024, Номер 13(27)

Опубликована: Май 30, 2024

Abstract Cancer immunotherapy is used to treat tumors by modulating the immune system. Although anticancer efficacy of cancer has been evaluated prior clinical trials, conventional in vivo animal and endpoint models inadequately replicate intricate process tumor elimination reflect human‐specific systems. Therefore, more sophisticated that mimic complex tumor‐immune microenvironment must be employed assess effectiveness immunotherapy. Additionally, using real‐time imaging technology, a step‐by‐step evaluation can applied, allowing for precise assessment treatment efficacy. Here, an overview various imaging‐based platforms recently developed immunotherapeutic applications presented. Specifically, fundamental technique discussed stably observing cell‐based cell killing direct imaging, microwell reproduces confined space spatial observation, droplet assay facilitates cell–cell interactions, 3D microphysiological system reconstructs vascular environment. Furthermore, it suggested future pursue human‐like

Язык: Английский

Процитировано

3

Advancements in Kidney-on-Chip: Antibiotic-Induced Kidney Injury and Future Directions DOI
Minhyeok Kim, Yugyeong Lee,

Gwang Myeong Seo

и другие.

BioChip Journal, Год журнала: 2024, Номер unknown

Опубликована: Июнь 27, 2024

Язык: Английский

Процитировано

3

Open Microfluidic Cell Culture in Hydrogels Enabled by 3D-Printed Molds DOI Creative Commons

MM O'Brien,

Ashley N. Spirrison,

Melati S. Abdul Halim

и другие.

Bioengineering, Год журнала: 2025, Номер 12(2), С. 102 - 102

Опубликована: Янв. 22, 2025

Cell culture models with tissue-mimicking architecture enable thein vitro investigation of cellular behavior and cell–cell interactions. These can recapitulate the structure function physiological systems be leveraged to elucidate mechanisms disease. In this work, we developed a method create open microfluidic cell cultures in using 3D-printed molds. The improves sample accessibility, is simpler manufacture than traditional closed requires minimal specialized equipment, making it an attractive for applications. Further, these molds generate multiple structures various hydrogels, including blood vessel mimics endothelial cells (HUVECs). Various geometries were patterned into agarose, gelatin, collagen type I star-shaped wells, square round channels, demonstrate versatility approach. Open channels created diameters ranging from 400 µm 4 mm densities 2 mg/mL mg/mL. To applicability our approach tissue modeling, generated 800 mm, high viability (>89%) both dimensions. used study effects hypoxia on CD31 expression by subjecting them reduced-O2 environment (∼16% O2). As compared normoxia conditions, under had reduction 8.3% surface 7.4%. Overall, enables generation different hydrogels development biological

Язык: Английский

Процитировано

0

Engineered Blood Clots for Embolization in a 3D Microfluidic Chip Model Mimicking Vascular Structures DOI
Jiwon Kim, J. Yang, Suyeon Ahn

и другие.

BioChip Journal, Год журнала: 2025, Номер unknown

Опубликована: Май 20, 2025

Язык: Английский

Процитировано

0