The
high
mortality
associated
with
certain
cancers
can
be
attributed
to
the
invasive
nature
of
tumor
cells.
Yet,
complexity
studying
invasion
hinders
our
understanding
how
spreads.
This
work
presents
a
microengineered
three-dimensional
(3D)
in
vitro
model
for
cancer
cell
and
interaction
endothelial
was
generated
by
printing
biomimetic
hydrogel
scaffold
directly
on
chip
using
2-photon
polymerization
that
simulates
brain's
extracellular
matrix.
scaffold's
geometry
specifically
designed
facilitate
growth
continuous
layer
cells
one
side,
while
also
allowing
introduction
other
side.
arrangement
confines
spatially
enables
situ
microscopy
as
they
invade
interact
layer.
We
examined
impact
3D
parameters
hydrogel's
physical
properties
used
patient
derived
glioblastoma
study
their
effect
invasion.
Notably,
tended
infiltrate
faster
when
an
barrier
present.
potential
adjusting
properties,
coupled
capability
real-time
observation
tumor-endothelial
interactions,
offers
platform
tumor–endothelial
interactions.
BioChip Journal,
Год журнала:
2024,
Номер
18(3), С. 345 - 356
Опубликована: Май 14, 2024
Abstract
The
vascular
system,
essential
for
human
physiology,
is
vital
transporting
nutrients,
oxygen,
and
waste.
Since
structures
are
involved
in
various
disease
pathogeneses
exhibit
different
morphologies
depending
on
the
organ,
researchers
have
endeavored
to
develop
organ-specific
models.
While
animal
models
possess
sophisticated
morphologies,
they
significant
discrepancies
from
tissues
due
species
differences,
which
limits
their
applicability.
To
overcome
limitations
arising
these
oversimplification
of
2D
dish
cultures,
microphysiological
systems
(MPS)
emerged
as
a
promising
alternative.
These
more
accurately
mimic
microenvironment
by
incorporating
cell
interactions,
physical
stimuli,
extracellular
matrix
components,
thus
facilitating
enhanced
tissue
differentiation
functionality.
Importantly,
MPS
often
utilize
human-derived
cells,
greatly
reducing
disparities
between
model
patient
responses.
This
review
focuses
recent
advancements
MPS,
particularly
modeling
discusses
potential
biological
adaptation.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(6), С. 2763 - 2763
Опубликована: Март 19, 2025
Glioblastomas
(GBMs),
among
the
most
aggressive
and
resilient
brain
tumors,
characteristically
exhibit
high
angiogenic
potential,
leading
to
formation
of
a
dense
yet
aberrant
vasculature,
both
morphologically
functionally.
With
these
premises,
numerous
expectations
were
initially
placed
on
anti-angiogenic
therapies,
soon
dashed
by
their
limited
efficacy
in
concretely
improving
patient
outcomes.
Neovascularization
GBM
emerged
as
complex,
dynamic,
heterogeneous
process,
hard
manage
with
classical
standard
care.
Growing
evidence
has
revealed
existence
non-canonical
strategies
angiogenesis,
variously
exploited
meet
its
ever-increasing
metabolic
demand
differently
involved
tumor
progression,
recurrence,
escape
from
treatments.
In
this
review,
we
provide
an
accurate
description
each
neovascularization
mode
encountered
tumors
date,
highlighting
molecular
players
signaling
cascades
primarily
involved.
We
also
detail
key
architectural
functional
aspects
characteristic
vascular
compartment
because
intricate
crosstalk
between
different
networks.
Additionally,
explore
repertoire
emerging
therapies
against
that
are
currently
under
study,
concluding
question:
faced
such
challenging
scenario,
could
combined
tailored
patient’s
genetic
signatures,
represent
effective
game
changer?
Sensors,
Год журнала:
2024,
Номер
24(9), С. 2865 - 2865
Опубликована: Апрель 30, 2024
Glioblastoma,
an
aggressive
primary
brain
tumor,
poses
a
significant
challenge
owing
to
its
dynamic
and
intricate
tumor
microenvironment.
This
review
investigates
the
innovative
integration
of
biosensor-enhanced
organ-on-a-chip
(OOC)
models
as
novel
strategy
for
in-depth
exploration
glioblastoma
microenvironment
dynamics.
In
recent
years,
transformative
approach
incorporating
biosensors
into
OOC
platforms
has
enabled
real-time
monitoring
analysis
cellular
behaviors
within
controlled
Conventional
in
vitro
vivo
exhibit
inherent
limitations
accurately
replicating
complex
nature
progression.
addresses
existing
research
gap
by
pioneering
models,
providing
comprehensive
platform
investigating
The
applications
this
combined
studying
dynamics
are
critically
scrutinized,
emphasizing
potential
bridge
between
simplistic
conditions.
Furthermore,
article
discusses
implications
elucidating
features
microenvironment,
encompassing
cell
migration,
proliferation,
interactions.
By
furnishing
insights,
these
significantly
contribute
unraveling
biology
glioblastoma,
thereby
influencing
development
more
accurate
diagnostic
therapeutic
strategies.
Biofabrication,
Год журнала:
2023,
Номер
15(4), С. 045016 - 045016
Опубликована: Авг. 11, 2023
Despite
the
advantages
of
microfluidic
system
in
drug
screening,
vascular
systems
responsible
for
transport
drugs
and
nutrients
have
been
hardly
considered
microfluidic-based
chemotherapeutic
screening.
Considering
physiological
characteristics
highly
vascularized
urinary
tumors,
we
here
investigated
response
bladder
tumor
cells
using
a
on
chip.
The
chip
was
designed
to
open-top
region
sample
introduction
hydrophilic
rail
spontaneous
hydrogel
patterning,
which
contributed
construction
tumor-hydrogel-endothelium
interfaces
spatiotemporal
on-demand
manner.
Utilizing
where
intravascularly
injected
cisplatin
diffuse
across
endothelium
into
samples,
responses
cisplatin-resistant
or
-susceptible
were
evaluated,
showing
preservation
cellular
resistance
even
within
structure
also
enabled
direct
harvest
samples
post
analysis
terms
secretome
gene
expressions.
Comparing
efficacy
presence
absence
endothelium,
found
that
proliferation
rates
increased
vasculature-incorporated
These
suggested
our
allows
establishment
vascular-gel-tumor
manners
further
enables
investigations
Bioengineering,
Год журнала:
2025,
Номер
12(2), С. 102 - 102
Опубликована: Янв. 22, 2025
Cell
culture
models
with
tissue-mimicking
architecture
enable
thein
vitro
investigation
of
cellular
behavior
and
cell–cell
interactions.
These
can
recapitulate
the
structure
function
physiological
systems
be
leveraged
to
elucidate
mechanisms
disease.
In
this
work,
we
developed
a
method
create
open
microfluidic
cell
cultures
in
using
3D-printed
molds.
The
improves
sample
accessibility,
is
simpler
manufacture
than
traditional
closed
requires
minimal
specialized
equipment,
making
it
an
attractive
for
applications.
Further,
these
molds
generate
multiple
structures
various
hydrogels,
including
blood
vessel
mimics
endothelial
cells
(HUVECs).
Various
geometries
were
patterned
into
agarose,
gelatin,
collagen
type
I
star-shaped
wells,
square
round
channels,
demonstrate
versatility
approach.
Open
channels
created
diameters
ranging
from
400
µm
4
mm
densities
2
mg/mL
mg/mL.
To
applicability
our
approach
tissue
modeling,
generated
800
mm,
high
viability
(>89%)
both
dimensions.
used
study
effects
hypoxia
on
CD31
expression
by
subjecting
them
reduced-O2
environment
(∼16%
O2).
As
compared
normoxia
conditions,
under
had
reduction
8.3%
surface
7.4%.
Overall,
enables
generation
different
hydrogels
development
biological
Advanced Healthcare Materials,
Год журнала:
2024,
Номер
13(27)
Опубликована: Май 30, 2024
Abstract
Cancer
immunotherapy
is
used
to
treat
tumors
by
modulating
the
immune
system.
Although
anticancer
efficacy
of
cancer
has
been
evaluated
prior
clinical
trials,
conventional
in
vivo
animal
and
endpoint
models
inadequately
replicate
intricate
process
tumor
elimination
reflect
human‐specific
systems.
Therefore,
more
sophisticated
that
mimic
complex
tumor‐immune
microenvironment
must
be
employed
assess
effectiveness
immunotherapy.
Additionally,
using
real‐time
imaging
technology,
a
step‐by‐step
evaluation
can
applied,
allowing
for
precise
assessment
treatment
efficacy.
Here,
an
overview
various
imaging‐based
platforms
recently
developed
immunotherapeutic
applications
presented.
Specifically,
fundamental
technique
discussed
stably
observing
cell‐based
cell
killing
direct
imaging,
microwell
reproduces
confined
space
spatial
observation,
droplet
assay
facilitates
cell–cell
interactions,
3D
microphysiological
system
reconstructs
vascular
environment.
Furthermore,
it
suggested
future
pursue
human‐like
