Journal of Controlled Release, Год журнала: 2024, Номер 373, С. 520 - 532
Опубликована: Июль 26, 2024
Язык: Английский
Frontiers in Molecular Biosciences, Год журнала: 2024, Номер 10
Опубликована: Янв. 3, 2024
Extracellular vesicles (EVs) are membrane-enclosed particles secreted by a variety of cell types. These encapsulate diverse range molecules, including proteins, nucleic acids, lipids, metabolites, and even organelles derived from their parental cells. While EVs have emerged as crucial mediators intercellular communication, they also hold immense potential both biomarkers therapeutic agents for numerous diseases. A thorough understanding EV biogenesis is the development EV-based diagnostic developments since composition can reflect health disease status donor cell. Moreover, when taken up target cells, exert profound effects on gene expression, signaling pathways, cellular behavior, which makes these biomolecules enticing targets interventions. Yet, despite decades research, intricate processes underlying cells subsequent uptake recipient remain poorly understood. In this review, we aim to summarize current insights advancements in mechanisms EVs. By shedding light fundamental governing delivery, review underscores basic mechanistic research pave way developing novel strategies applications.
Язык: Английский
Процитировано
28
Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)
Опубликована: Фев. 10, 2025
Alzheimer's disease (AD) is a distinctive form of dementia characterized by age-related cognitive decline and memory impairment. A key hallmark AD the irreversible overaccumulation beta-amyloid (Aβ) in brain, associated with neuroinflammation neuronal death. Although Aβ clearance immunoregulation have been major therapeutic strategies for AD, highly selective transport across blood–brain barrier (BBB) negatively affects delivery efficacy drugs without ability to cross BBB. In this review, we discuss potential lipid-based nanoparticles (LBNs) as promising vehicles drug treatment. LBNs, composed phospholipid mono- or bilayer, attracted attention due their exceptional cellular penetration capabilities loading capabilities, which also facilitate cargo transcytosis Recent advances development engineering LBNs overcome existing limitations current clinical approaches treatment addressing off-target effects low efficacy. Here, review pathways BBB, well various types therapy, including exosomes, liposomes, solid lipid (SLNs), nanostructured carriers (NLCs), elucidate properties, preparation methodologies, efficacy, thereby offering innovative avenues novel translation therapy.
Язык: Английский
Процитировано
3
Nano Convergence, Год журнала: 2023, Номер 10(1)
Опубликована: Авг. 7, 2023
Abstract Cancer immunotherapy, which harnesses the power of immune system, has shown immense promise in fight against malignancies. Messenger RNA (mRNA) stands as a versatile instrument this context, with its capacity to encode tumor-associated antigens (TAAs), cell receptors, cytokines, and antibodies. Nevertheless, inherent structural instability mRNA requires development effective delivery systems. Lipid nanoparticles (LNPs) have emerged significant candidates for cancer providing both protection enhanced intracellular efficiency. In review, we offer comprehensive summary recent advancements LNP-based systems, focus on strategies optimizing design mRNA-encoded therapeutics treatment. Furthermore, delve into challenges encountered field contemplate future perspectives, aiming improve safety efficacy immunotherapies. Graphical
Язык: Английский
Процитировано
39
Cancers, Год журнала: 2023, Номер 15(10), С. 2838 - 2838
Опубликована: Май 19, 2023
Extracellular vesicles (EVs) are lipid bilayer-enclosed bodies secreted by all cell types. EVs carry bioactive materials, such as proteins, lipids, metabolites, and nucleic acids, to communicate elicit functional alterations phenotypic changes in the counterpart stromal cells. In cancer, cells secrete shape a tumor-promoting niche. Tumor-secreted mediate communications with immune that determine fate of anti-tumor therapeutic effectiveness. Surface engineering has emerged promising tool for modulation tumor microenvironments cancer immunotherapy. Modification EVs' surface various molecules, antibodies, peptides, can enhance their targeting specificity, immunogenicity, biodistribution, pharmacokinetics. The diverse approaches sought EV surfaces be categorized physical, chemical, genetic strategies. choice method depends on specific application desired outcome. Each its advantages disadvantages. This review lends bird's-eye view recent progress these respect rational implications immunomodulation (TME) from pro-tumorigenic anti-tumorigenic ones. strategies modulating TME using targeted EVs, advantages, current limitations, future directions discussed.
Язык: Английский
Процитировано
34
International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 2241 - 2264
Опубликована: Март 1, 2024
Abstract: Recently, gene therapy has become a subject of considerable research and been widely evaluated in various disease models. Though it is considered as stand-alone agent for COVID-19 vaccination, still suffering from the following drawbacks during its translation bench to bedside: high sensitivity exogenous nucleic acids enzymatic degradation; severe side effects induced either by or components formulation; difficulty cross barriers before reaching therapeutic target. Therefore, successful application therapy, safe reliable transport vector urgently needed. Extracellular vesicles (EVs) are ideal candidate delivery drugs owing their low immunogenicity, good biocompatibility toxicity. To better understand properties EVs advantages drug vehicles, this review covers origin methods generation, well common isolation purification research, with pros cons discussed. Meanwhile, engineering also highlighted. In addition, paper presents progress EVs-mediated microRNAs, small interfering RNAs, messenger plasmids, antisense oligonucleotides. We believe will provide theoretical basis development drugs. Keywords: extracellular vesicles, system,
Язык: Английский
Процитировано
11
Theranostics, Год журнала: 2024, Номер 14(4), С. 1631 - 1646
Опубликована: Янв. 1, 2024
Diabetic retinopathy (DR), a complex complication of diabetes mellitus (DM), is leading cause adult blindness.Hyperglycemia triggers DR, resulting in microvascular damage, glial apoptosis, and neuronal degeneration.Inflammation oxidative stress play crucial roles during this process.Current clinical treatments for DR primarily target the advanced retinal disorder but offer limited benefits with inevitable side effects.Extracellular vesicles (EVs) exhibit unique morphological features, contents, biological properties can be found cell culture supernatants, various body fluids, tissues.In EVs specific cargo composition would induce reaction receptor once internalized, mediating cellular communication disease progression.Increasing evidence indicates that monitoring changes EV quantity content aid diagnosis prognosis.Furthermore, extensive research investigating potential these nanoparticles as effective therapeutic agents preclinical models DR.This review explores current understanding pathological effects development, discusses their biomarkers strategies, paves way further advancements.
Язык: Английский
Процитировано
9
RSC Advances, Год журнала: 2024, Номер 14(42), С. 30807 - 30829
Опубликована: Янв. 1, 2024
Modified exosomes are a smart tool for the upcoming precision cancer therapeutic era.
Язык: Английский
Процитировано
7
Stem Cell Research & Therapy, Год журнала: 2024, Номер 15(1)
Опубликована: Сен. 3, 2024
Abstract Rheumatoid arthritis (RA) remains a challenging chronic autoimmune disorder characterized by persistent joint inflammation and damage. While modern regenerative strategies, encompassing cell/stem cell-based therapies, gene therapy, tissue engineering, have advanced repair efforts, definitive cure for RA elusive. Consequently, there is growing interest in developing targeted therapies that directly address the underlying mechanisms driving pathogenesis, such as extracellular vesicles (EVs). These small membrane-bound particles can modulate immune responses within inflammatory microenvironment of damaged cartilage. To launch clinical potential EVs, they be isolated from various cell types through several techniques. EVs carry bioactive molecules anti-inflammatory or pro-regenerative drugs, deliver them to affected joints, affect behavior injured cells, making compelling choice therapy drug delivery patients. However, are still challenges limitations associated with EV-based including absence standardized protocols EV isolation, characterization, delivery. This review provides comprehensive overview cellular sources delves into their therapeutic hurdles must overcome.
Язык: Английский
Процитировано
5
Carbohydrate Polymers, Год журнала: 2023, Номер 327, С. 121634 - 121634
Опубликована: Ноя. 28, 2023
Язык: Английский
Процитировано
12
Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)
Опубликована: Апрель 15, 2025
Cancer immunotherapy aims to harness the body's own immune system for effective and long-lasting elimination of malignant neoplastic tissues. Owing advance in understanding cancer pathology immunology, many novel strategies enhancing immunological responses against various cancers have been successfully developed, some translated into excellent clinical outcomes. As one promising strategy next generation immunotherapies, activating multi-cellular network (MCN) within tumor microenvironment (TME) deploy multiple mechanisms action (MOAs) has attracted significant attention. To achieve this effectively safely, delivering or pleiotropic therapeutic cargoes targeted sites cancerous tissues, cells, intracellular organelles is critical, which numerous nanocarriers developed leveraged. In review, we first introduce payloads categorized according their predicted functions physicochemical structures forms. Then, nanocarriers, along with unique characteristics, properties, advantages, limitations, are introduced notable recent applications immunotherapy. Following discussions on targeting strategies, a summary each nanocarrier matching suitable provided comprehensive background information designing regimens.
Язык: Английский
Процитировано
0