Microbiology Spectrum,
Год журнала:
2023,
Номер
unknown
Опубликована: Ноя. 29, 2023
ABSTRACT
IncF
plasmids
are
commonly
found
in
extra-intestinal
pathogenic
Escherichia
coli
(ExPEC)
strains,
serving
as
reservoirs
for
antimicrobial
resistance
(AMR)
genes
and
virulence
factors,
persistently
coexisting
with
ExPEC
lineages.
Multidrug-resistant
(MDR)
high-risk
clones,
particularly
ST131,
ST1193,
ST410,
have
acquired
diverse
over
time,
containing
various
AMR
determinants,
contributing
significantly
to
their
global
success.
However,
the
broader
roles
of
these
success
MDR
beyond
AMR,
remain
elusive.
In
this
study,
we
employed
a
novel
clustered
regularly
interspaced
short
palindromic
repeats–CRISPR-associated
protein-9
nuclease
(CRISPR-Cas9)-mediated
pCasCure
plasmid-curing
system
precisely
remove
specific
among
clones
(ST1193,
ST410).
Antibiotic-resistant
parent
strains
reverted
antibiotic-susceptible
states
post-curing;
however,
plasmid
curing
did
not
show
significant
impact
on
bacterial
vitro
growth
had
little
other
phenotypes,
including
survival
water,
dry
environment
biofilm
production.
addition,
affect
conjugation
frequency
KPC-producing
pKpQIL
plasmid.
This
study
represents
pivotal
initial
step
understanding
precise
ExPEC.
Future
research
will
be
crucial
investigating
influence
cell
invasion
vivo
fitness,
thereby
providing
more
comprehensive
perspective
functions
clones.
IMPORTANCE
Understanding
role
drug-resistant
bacteria
has
far-reaching
implications
tackling
antibiotic
resistance.
The
study's
use
CRISPR-Cas9-mediated
provides
precision
tool
dissecting
especially
high-risk,
multidrug-resistant
like
ST410.
offers
stepping
stone
future
into
how
complex
aspects
behavior,
such
fitness.
Antimicrobial Agents and Chemotherapy,
Год журнала:
2024,
Номер
68(2)
Опубликована: Янв. 9, 2024
sequence
type
ST410
is
an
emerging
carbapenemase-producing
multidrug-resistant
(MDR)
high-risk
One-Health
clone
with
the
potential
to
significantly
increase
carbapenem
resistance
among
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Июль 10, 2024
Abstract
Extraintestinal
Pathogenic
Escherichia
coli
(ExPEC)
pose
a
significant
threat
to
human
and
animal
health.
However,
the
diversity
antibiotic
resistance
of
ExPEC,
their
connection
infections,
remain
largely
unexplored.
The
study
performs
large-scale
genome
sequencing
testing
499
swine-derived
ExPEC
isolates
from
China.
Results
show
swine
are
phylogenetically
diverse,
with
over
80%
belonging
phylogroups
B1
A.
Importantly,
15
exhibit
genetic
relatedness
human-origin
E.
strains.
Additionally,
49
strains
harbor
toxins
typical
enteric
pathotypes,
implying
hybrid
pathotypes.
Notably,
97%
total
multidrug
resistant,
including
critical
drugs
like
third-
fourth-generation
cephalosporins.
Correspondingly,
genomic
analysis
unveils
prevalent
genes
(ARGs),
often
associated
co-transfer
mechanisms.
Furthermore,
20
complete
genomes
illuminates
transmission
pathways
ARGs
within
pathogens.
For
example,
plasmids
co-harboring
fosA3
,
bla
CTX-M-14
mcr-1
between
Salmonella
enterica
is
observed.
These
findings
underscore
importance
monitoring
controlling
infections
in
animals,
as
they
can
serve
reservoir
potential
affect
health
or
even
be
origin
pathogens
infecting
humans.
npj Antimicrobials and Resistance,
Год журнала:
2024,
Номер
2(1)
Опубликована: Март 18, 2024
Abstract
Water
quality
testing
does
not
recognise
antimicrobial
resistance
(AMR)
and
is
often
limited
to
indicators
of
faecal
contamination
Escherichia
coli
Enterococcus
species.
In
Europe,
data
on
AMR
in
drinking
water
scarce.
Ireland,
as
many
countries,
household
supplied
via
mains
or
private
wells
schemes.
Using
citizen
science,
we
identified
Irish
supplies
reservoirs
resistant
bacteria
(ARB).
Gram-negative
(
n
=
464)
Gram-positive
72)
were
isolated.
We
instances
potentially
opportunistic
ARB
such
Enterobacter
cloacae,
Acinetobacter
baumannii
report
multidrug
casseliflavus,
E.
coli,
Stenotrophomonas
maltophilia
,
Serratia
rubidaea
.
also
linezolid-resistant
water.
Linezolid
a
last-resort
antibiotic
used
treat
vancomycin-resistant
sp.
Additionally,
mobile
three
samples,
two
which
carried
IncF
group,
one
IncQ
five
Col-like
plasmids.
Our
work
suggests
that
potential
sink
source
pathogens.
This
highlights
value
surveillance
One
Health
framework
the
would
provide
information
regarding
movement
persistence
ARGs
are
able
survive
subsequently
have
opportunity
be
mobilised
through
humans;
linking
environment
human
threatening
health.
Journal of Clinical Microbiology,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 6, 2025
ABSTRACT
Enterobacterales
(mostly
Klebsiella
pneumoniae
,
Escherichia
coli
)
with
OXA-48-like
carbapenemases
(e.g.,
OXA-48,
-181,
-232,
-244)
are
undermining
the
global
efficiency
of
carbapenem
therapy.
In
Middle
East,
North
Africa,
and
some
European
countries,
most
common
types
among
.
Currently,
OXA-48
is
endemic
in
Spain,
France,
Belgium;
OXA-181
Sub-Saharan
Africa
Indian
Subcontinent,
while
OXA-232
has
been
increasing
Subcontinent.
countries
Germany,
Denmark,
Switzerland,
France)
experiencing
community
outbreaks
E.
ST38
that
produce
OXA-244,
these
strains
have
introduced
into
Norwegian,
Polish,
Czech
hospitals.
The
ascendancy
genes
due
to
combination
horizontal
spread
through
promiscuous
plasmids
IncL,
IncX3,
ColE2)
vertical
certain
high-risk
multidrug-resistant
clones
K.
ST14,
ST15,
ST147,
ST307;
ST38,
ST410).
This
a
powerful
“gene
survival
strategy”
assisted
different
environments
including
setting.
laboratory
diagnosis
complex;
therefore,
bacteria
“difficult
detect”
variants
OXA-484)
likely
underreported
spreading
silently
“beneath
radar”
hospital
settings.
forces
be
reckoned
with.
EBioMedicine,
Год журнала:
2024,
Номер
103, С. 105097 - 105097
Опубликована: Апрель 11, 2024
Extended-spectrum
cephalosporins
(ESCs)
are
third
and
fourth
generation
cephalosporin
antimicrobials
used
in
humans
animals
to
treat
infections
due
multidrug-resistant
(MDR)
bacteria.
Resistance
ESCs
(ESC-R)
Enterobacterales
is
predominantly
the
production
of
extended-spectrum
β-lactamases
(ESBLs)
plasmid-mediated
AmpC
(AmpCs).
The
dynamics
ESBLs
AmpCs
changing
across
countries
host
species,
result
global
transmission
ESC-R
genes.
Plasmids
known
play
a
key
role
this
dissemination,
but
relative
importance
different
types
plasmids
not
fully
understood.
In
study,
Escherichia
coli
with
major
genes
blaCTX-M-1,
blaCTX-M-15,
blaCTX-M-14
blaCMY-2
(AmpC),
were
selected
from
diverse
species
other
sources
Canada,
France
Germany,
collected
between
2003
2017.
To
examine
detail
vehicles
genes,
long-
short-read
sequences
generated
obtain
complete
contiguous
chromosome
plasmid
(n
=
192
E.
coli).
types,
gene
composition
genetic
relatedness
these
investigated,
along
association
isolate
year,
source
geographical
origin,
put
context
publicly
available
sequences.
We
identified
five
epidemic
resistance
subtypes
distinct
properties
that
associated
dissemination
multiple
lineages
species.
IncI1
pST3
blaCTX-M-1
subtype
was
found
more
than
main
subtypes,
whereas
pST12
frequent
Canadian
German
human
chicken
isolates.
Clonal
expansion
also
contributed
ST131
ST117
harbouring
plasmid.
pST2
predominant
isolates
France,
while
IncF
F31:A4:B1
blaCTX-M-15
F2:A-:B-
cattle
countries.
Beyond
their
nature
respect
our
collection
almost
all
carried
antimicrobial
(AMR)
conferring
classes.
Finally,
we
signatures
regions
surrounding
specific
identifying
mechanisms
movement,
using
databases,
widespread
bacterial
continents.
provide
evidence
contribute
addition,
some
confer
success
suggests
they
may
have
fitness
advantage
over
subtypes.
Identification
understanding
AMR
crucial
develop
target
strategies
interventions
reduce
spread
AMR.
This
project
supported
by
Joint
Programming
Initiative
on
Antimicrobial
(JPIAMR),
through
Medical
Research
Council
(MRC,
MR/R000948/1),
Institutes
Health
(CFC-150770),
Genomics
Development
(Government
Canada),
Federal
Ministry
Education
(BMBF)
grant
no.
01KI1709,
French
Agency
for
food
environmental
occupational
health
&
safety
(Anses),
National
Reference
Center
(CNR)
resistance.
Support
provided
Biotechnology
Biological
Sciences
(BBSRC)
BBSRC
Institute
Strategic
Programme
Microbes
Food
ChainBB/R012504/1
its
constituent
BBS/E/F/000PR10348
(Theme
1,
Epidemiology
Evolution
Pathogens
Chain).
Antibiotics,
Год журнала:
2024,
Номер
13(4), С. 363 - 363
Опубликована: Апрель 16, 2024
Extended-spectrum
β-lactamase-producing
Escherichia
coli
ST131
has
become
widespread
worldwide.
This
study
aims
to
characterize
the
virulome,
resistome,
and
population
structure
of
E.
isolates
from
clinical
blood
samples
in
Hungary.
A
total
30
C2/H30Rx
33
C1-M27
were
selected
for
Illumina
MiSeq
sequencing
MinION
sequencing,
followed
by
hybrid
de
novo
assembly.
Five
one
cluster
identified.
harbored
F1:A2:B20
plasmid
93.9%
cases.
Long-read
revealed
that
blaCTX-M-27
was
on
plasmids.
Among
isolates,
only
six
carried
C2-associated
F2:A1:B-
type.
Of
19
hybrid-assembled
genomes,
blaCTX-M-15
gene
located
isolate,
while
other
ISEcp1
or
IS26-mediated
chromosomal
integration
detected
unique
variations.
In
isolate
a
part
integrated
into
chromosome.
These
results
suggest
CTX-M-15-producing
CTX-M-27-producing
subclades
may
have
emerged
spread
different
ways
While
mainly
C1/H30R-associated
plasmid,
IncF-like
plasmids
its
composite
transposons
been
incorporated
chromosome
through
convergent
evolutionary
processes.
Biomedicine & Pharmacotherapy,
Год журнала:
2023,
Номер
168, С. 115703 - 115703
Опубликована: Окт. 17, 2023
Antimicrobial
resistance
is
a
worldwide
problem
that
urges
novel
alternatives
to
treat
infections.
In
attempts
find
molecules,
we
assess
the
antimicrobial
potential
of
seven
essential
oils
(EO)
different
plants
(Pinus
sylvestris,
Citrus
limon,
Origanum
vulgare,
Cymbopogon
martini,
Cinnamomum
cassia,
Melaleuca
alternifolia
and
Eucalyptus
globulus)
against
two
multidrug-resistant
bacteria
species,
i.e.
Neisseria
gonorrhoeae
Streptococcus
suis.
EOs
P.
sylvestris
C.
limon
revealed
higher
bactericidal
activity
(MIC
≤
0.5
mg/mL)
capacity
rapidly
disperse
biofilms
several
N.
clinical
isolates
than
other
EOs.
Examination
exposed
both
EO
by
electron
microscopy
reduction
bacterial
aggregates,
high
production
extracellular
vesicles,
alteration
cell
integrity.
This
was
dose-dependent
enhanced
in
DNase
I-treated
biofilms.
Antibiotic
susceptibility
studies
confirmed
affected
outer
membrane
permeability,
analysis
EO-
an
LPS-deficient
mutant
suggested
target
LPS
bilayer.
Further
α-
β-pinene
d-limonene,
components
EO,
contribute
such
activity.
O.
vulgare
exhibited
promising
S.
suis,
but
only
biofilm
dispersal
activity,
which
also
studies.
To
conclude,
studied
this
work
exhibit
anti-biofilm
activities
gonococcus
streptococcus,
respectively.
Clinical Microbiology Reviews,
Год журнала:
2024,
Номер
37(4)
Опубликована: Ноя. 15, 2024
SUMMARYThe
carbapenems
remain
some
of
the
most
effective
options
available
for
treating
patients
with
serious
infections
due
to
Gram-negative
bacteria.
Carbapenemases
are
enzymes
that
hydrolyze
and
primary
method
driving
carbapenem
resistance
globally.
Detection
carbapenemases
is
required
patient
management,
rapid
implementation
infection
prevention
control
(IP&C)
protocols,
epidemiologic
purposes.
Therefore,
clinical
public
health
microbiology
laboratories
must
be
able
detect
report
among
predominant
organisms
from
both
cultured
isolates
direct
specimens
treatment
surveillance
There
not
a
"one
size
fits
all"
laboratory
approach
detection
bacteria
carbapenemases,
institutions
need
determine
what
best
goals
their
antimicrobial
stewardship
IP&C
programs.
Luckily,
there
several
approaches
choose
methods
suits
individual
needs.
A
bacterial
consists
two
steps,
namely
screening
process
(
The
global
upsurge
in
antibiotic
resistant
bacteria
(ARB)
is
putting
immense
pressure
on
healthcare.
spreading
of
antimicrobial
resistance
facilitated
by
mobile
genetic
elements,
most
especially
plasmids.
widespread
use
antibiotics
clinical
and
veterinary
environments
creates
selective
that
drives
the
evolution
ARB.
Plasmids
contribute
to
propagation
AR
different
types
infections.
role
plasmids
play
this
necessitates
their
utilization
molecular
surveillance
detect
emergence
ARB
track
spread
Recent
technologies
like
replicon
typing
whole
genome
sequencing
(WGS)
have
become
gold
standard
for
epidemiology
detection
control
epidemics
settings.
Unfortunately,
access
such
limited
low-
middle-income
countries
(LMICs).
major
aim
review
examine
specific
contributions
settings
elucidate
various
been
attributed
antibiotic-resistant
infections
healthcare
Healthcare
LMICs
should
be
supported
build
capacity
WGS
effectively
prevent
bacterial