Escherichia coli sequence type 410 with carbapenemases: a paradigm shift within E. coli toward multidrug resistance

Antimicrobial Agents and Chemotherapy, Год журнала: 2024, Номер 68(2)

Опубликована: Янв. 9, 2024

sequence type ST410 is an emerging carbapenemase-producing multidrug-resistant (MDR) high-risk One-Health clone with the potential to significantly increase carbapenem resistance among

Язык: Английский

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Population structure and antibiotic resistance of swine extraintestinal pathogenic Escherichia coli from China

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Июль 10, 2024

Abstract Extraintestinal Pathogenic Escherichia coli (ExPEC) pose a significant threat to human and animal health. However, the diversity antibiotic resistance of ExPEC, their connection infections, remain largely unexplored. The study performs large-scale genome sequencing testing 499 swine-derived ExPEC isolates from China. Results show swine are phylogenetically diverse, with over 80% belonging phylogroups B1 A. Importantly, 15 exhibit genetic relatedness human-origin E. strains. Additionally, 49 strains harbor toxins typical enteric pathotypes, implying hybrid pathotypes. Notably, 97% total multidrug resistant, including critical drugs like third- fourth-generation cephalosporins. Correspondingly, genomic analysis unveils prevalent genes (ARGs), often associated co-transfer mechanisms. Furthermore, 20 complete genomes illuminates transmission pathways ARGs within pathogens. For example, plasmids co-harboring fosA3 , bla CTX-M-14 mcr-1 between Salmonella enterica is observed. These findings underscore importance monitoring controlling infections in animals, as they can serve reservoir potential affect health or even be origin pathogens infecting humans.

Язык: Английский

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Private and well drinking water are reservoirs for antimicrobial resistant bacteria

npj Antimicrobials and Resistance, Год журнала: 2024, Номер 2(1)

Опубликована: Март 18, 2024

Abstract Water quality testing does not recognise antimicrobial resistance (AMR) and is often limited to indicators of faecal contamination Escherichia coli Enterococcus species. In Europe, data on AMR in drinking water scarce. Ireland, as many countries, household supplied via mains or private wells schemes. Using citizen science, we identified Irish supplies reservoirs resistant bacteria (ARB). Gram-negative ( n = 464) Gram-positive 72) were isolated. We instances potentially opportunistic ARB such Enterobacter cloacae, Acinetobacter baumannii report multidrug casseliflavus, E. coli, Stenotrophomonas maltophilia , Serratia rubidaea . also linezolid-resistant water. Linezolid a last-resort antibiotic used treat vancomycin-resistant sp. Additionally, mobile three samples, two which carried IncF group, one IncQ five Col-like plasmids. Our work suggests that potential sink source pathogens. This highlights value surveillance One Health framework the would provide information regarding movement persistence ARGs are able survive subsequently have opportunity be mobilised through humans; linking environment human threatening health.

Язык: Английский

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Rapidly spreading Enterobacterales with OXA-48-like carbapenemases

Journal of Clinical Microbiology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 6, 2025

ABSTRACT Enterobacterales (mostly Klebsiella pneumoniae , Escherichia coli ) with OXA-48-like carbapenemases (e.g., OXA-48, -181, -232, -244) are undermining the global efficiency of carbapenem therapy. In Middle East, North Africa, and some European countries, most common types among . Currently, OXA-48 is endemic in Spain, France, Belgium; OXA-181 Sub-Saharan Africa Indian Subcontinent, while OXA-232 has been increasing Subcontinent. countries Germany, Denmark, Switzerland, France) experiencing community outbreaks E. ST38 that produce OXA-244, these strains have introduced into Norwegian, Polish, Czech hospitals. The ascendancy genes due to combination horizontal spread through promiscuous plasmids IncL, IncX3, ColE2) vertical certain high-risk multidrug-resistant clones K. ST14, ST15, ST147, ST307; ST38, ST410). This a powerful “gene survival strategy” assisted different environments including setting. laboratory diagnosis complex; therefore, bacteria “difficult detect” variants OXA-484) likely underreported spreading silently “beneath radar” hospital settings. forces be reckoned with.

Язык: Английский

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Global transmission of extended-spectrum cephalosporin resistance in Escherichia coli driven by epidemic plasmids

EBioMedicine, Год журнала: 2024, Номер 103, С. 105097 - 105097

Опубликована: Апрель 11, 2024

Extended-spectrum cephalosporins (ESCs) are third and fourth generation cephalosporin antimicrobials used in humans animals to treat infections due multidrug-resistant (MDR) bacteria. Resistance ESCs (ESC-R) Enterobacterales is predominantly the production of extended-spectrum β-lactamases (ESBLs) plasmid-mediated AmpC (AmpCs). The dynamics ESBLs AmpCs changing across countries host species, result global transmission ESC-R genes. Plasmids known play a key role this dissemination, but relative importance different types plasmids not fully understood. In study, Escherichia coli with major genes blaCTX-M-1, blaCTX-M-15, blaCTX-M-14 blaCMY-2 (AmpC), were selected from diverse species other sources Canada, France Germany, collected between 2003 2017. To examine detail vehicles genes, long- short-read sequences generated obtain complete contiguous chromosome plasmid (n = 192 E. coli). types, gene composition genetic relatedness these investigated, along association isolate year, source geographical origin, put context publicly available sequences. We identified five epidemic resistance subtypes distinct properties that associated dissemination multiple lineages species. IncI1 pST3 blaCTX-M-1 subtype was found more than main subtypes, whereas pST12 frequent Canadian German human chicken isolates. Clonal expansion also contributed ST131 ST117 harbouring plasmid. pST2 predominant isolates France, while IncF F31:A4:B1 blaCTX-M-15 F2:A-:B- cattle countries. Beyond their nature respect our collection almost all carried antimicrobial (AMR) conferring classes. Finally, we signatures regions surrounding specific identifying mechanisms movement, using databases, widespread bacterial continents. provide evidence contribute addition, some confer success suggests they may have fitness advantage over subtypes. Identification understanding AMR crucial develop target strategies interventions reduce spread AMR. This project supported by Joint Programming Initiative on Antimicrobial (JPIAMR), through Medical Research Council (MRC, MR/R000948/1), Institutes Health (CFC-150770), Genomics Development (Government Canada), Federal Ministry Education (BMBF) grant no. 01KI1709, French Agency for food environmental occupational health & safety (Anses), National Reference Center (CNR) resistance. Support provided Biotechnology Biological Sciences (BBSRC) BBSRC Institute Strategic Programme Microbes Food ChainBB/R012504/1 its constituent BBS/E/F/000PR10348 (Theme 1, Epidemiology Evolution Pathogens Chain).

Язык: Английский

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Genomic Epidemiology of C2/H30Rx and C1-M27 Subclades of Escherichia coli ST131 Isolates from Clinical Blood Samples in Hungary

Antibiotics, Год журнала: 2024, Номер 13(4), С. 363 - 363

Опубликована: Апрель 16, 2024

Extended-spectrum β-lactamase-producing Escherichia coli ST131 has become widespread worldwide. This study aims to characterize the virulome, resistome, and population structure of E. isolates from clinical blood samples in Hungary. A total 30 C2/H30Rx 33 C1-M27 were selected for Illumina MiSeq sequencing MinION sequencing, followed by hybrid de novo assembly. Five one cluster identified. harbored F1:A2:B20 plasmid 93.9% cases. Long-read revealed that blaCTX-M-27 was on plasmids. Among isolates, only six carried C2-associated F2:A1:B- type. Of 19 hybrid-assembled genomes, blaCTX-M-15 gene located isolate, while other ISEcp1 or IS26-mediated chromosomal integration detected unique variations. In isolate a part integrated into chromosome. These results suggest CTX-M-15-producing CTX-M-27-producing subclades may have emerged spread different ways While mainly C1/H30R-associated plasmid, IncF-like plasmids its composite transposons been incorporated chromosome through convergent evolutionary processes.

Язык: Английский

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Essential oils of Pinus sylvestris, Citrus limon and Origanum vulgare exhibit high bactericidal and anti-biofilm activities against Neisseria gonorrhoeae and Streptococcus suis

Biomedicine & Pharmacotherapy, Год журнала: 2023, Номер 168, С. 115703 - 115703

Опубликована: Окт. 17, 2023

Antimicrobial resistance is a worldwide problem that urges novel alternatives to treat infections. In attempts find molecules, we assess the antimicrobial potential of seven essential oils (EO) different plants (Pinus sylvestris, Citrus limon, Origanum vulgare, Cymbopogon martini, Cinnamomum cassia, Melaleuca alternifolia and Eucalyptus globulus) against two multidrug-resistant bacteria species, i.e. Neisseria gonorrhoeae Streptococcus suis. EOs P. sylvestris C. limon revealed higher bactericidal activity (MIC ≤ 0.5 mg/mL) capacity rapidly disperse biofilms several N. clinical isolates than other EOs. Examination exposed both EO by electron microscopy reduction bacterial aggregates, high production extracellular vesicles, alteration cell integrity. This was dose-dependent enhanced in DNase I-treated biofilms. Antibiotic susceptibility studies confirmed affected outer membrane permeability, analysis EO- an LPS-deficient mutant suggested target LPS bilayer. Further α- β-pinene d-limonene, components EO, contribute such activity. O. vulgare exhibited promising S. suis, but only biofilm dispersal activity, which also studies. To conclude, studied this work exhibit anti-biofilm activities gonococcus streptococcus, respectively.

Язык: Английский

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Emergence of Salmonella Infantis carrying the pESI-like plasmid from eggs in egg grading and packing plants in Korea

Food Microbiology, Год журнала: 2024, Номер 122, С. 104568 - 104568

Опубликована: Май 25, 2024

Язык: Английский

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Laboratory detection of carbapenemases among Gram-negative organisms

Clinical Microbiology Reviews, Год журнала: 2024, Номер 37(4)

Опубликована: Ноя. 15, 2024

SUMMARYThe carbapenems remain some of the most effective options available for treating patients with serious infections due to Gram-negative bacteria. Carbapenemases are enzymes that hydrolyze and primary method driving carbapenem resistance globally. Detection carbapenemases is required patient management, rapid implementation infection prevention control (IP&C) protocols, epidemiologic purposes. Therefore, clinical public health microbiology laboratories must be able detect report among predominant organisms from both cultured isolates direct specimens treatment surveillance There not a "one size fits all" laboratory approach detection bacteria carbapenemases, institutions need determine what best goals their antimicrobial stewardship IP&C programs. Luckily, there several approaches choose methods suits individual needs. A bacterial consists two steps, namely screening process (

Язык: Английский

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Role of plasmids in antibiotic resistance in clinical infections and implications for epidemiological surveillance: a review

All Life, Год журнала: 2024, Номер 17(1)

Опубликована: Июнь 6, 2024

The global upsurge in antibiotic resistant bacteria (ARB) is putting immense pressure on healthcare. spreading of antimicrobial resistance facilitated by mobile genetic elements, most especially plasmids. widespread use antibiotics clinical and veterinary environments creates selective that drives the evolution ARB. Plasmids contribute to propagation AR different types infections. role plasmids play this necessitates their utilization molecular surveillance detect emergence ARB track spread Recent technologies like replicon typing whole genome sequencing (WGS) have become gold standard for epidemiology detection control epidemics settings. Unfortunately, access such limited low- middle-income countries (LMICs). major aim review examine specific contributions settings elucidate various been attributed antibiotic-resistant infections healthcare Healthcare LMICs should be supported build capacity WGS effectively prevent bacterial

Язык: Английский

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