Published: Jan. 1, 2024
Language: Английский
Journal of Clinical Microbiology, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 6, 2025
ABSTRACT Enterobacterales (mostly Klebsiella pneumoniae , Escherichia coli ) with OXA-48-like carbapenemases (e.g., OXA-48, -181, -232, -244) are undermining the global efficiency of carbapenem therapy. In Middle East, North Africa, and some European countries, most common types among . Currently, OXA-48 is endemic in Spain, France, Belgium; OXA-181 Sub-Saharan Africa Indian Subcontinent, while OXA-232 has been increasing Subcontinent. countries Germany, Denmark, Switzerland, France) experiencing community outbreaks E. ST38 that produce OXA-244, these strains have introduced into Norwegian, Polish, Czech hospitals. The ascendancy genes due to combination horizontal spread through promiscuous plasmids IncL, IncX3, ColE2) vertical certain high-risk multidrug-resistant clones K. ST14, ST15, ST147, ST307; ST38, ST410). This a powerful “gene survival strategy” assisted different environments including setting. laboratory diagnosis complex; therefore, bacteria “difficult detect” variants OXA-484) likely underreported spreading silently “beneath radar” hospital settings. forces be reckoned with.
Language: Английский
Citations
2
Antimicrobial Agents and Chemotherapy, Journal Year: 2024, Volume and Issue: 68(2)
Published: Jan. 9, 2024
sequence type ST410 is an emerging carbapenemase-producing multidrug-resistant (MDR) high-risk One-Health clone with the potential to significantly increase carbapenem resistance among
Language: Английский
Citations
14
Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: July 10, 2024
Abstract Extraintestinal Pathogenic Escherichia coli (ExPEC) pose a significant threat to human and animal health. However, the diversity antibiotic resistance of ExPEC, their connection infections, remain largely unexplored. The study performs large-scale genome sequencing testing 499 swine-derived ExPEC isolates from China. Results show swine are phylogenetically diverse, with over 80% belonging phylogroups B1 A. Importantly, 15 exhibit genetic relatedness human-origin E. strains. Additionally, 49 strains harbor toxins typical enteric pathotypes, implying hybrid pathotypes. Notably, 97% total multidrug resistant, including critical drugs like third- fourth-generation cephalosporins. Correspondingly, genomic analysis unveils prevalent genes (ARGs), often associated co-transfer mechanisms. Furthermore, 20 complete genomes illuminates transmission pathways ARGs within pathogens. For example, plasmids co-harboring fosA3 , bla CTX-M-14 mcr-1 between Salmonella enterica is observed. These findings underscore importance monitoring controlling infections in animals, as they can serve reservoir potential affect health or even be origin pathogens infecting humans.
Language: Английский
Citations
11
npj Antimicrobials and Resistance, Journal Year: 2024, Volume and Issue: 2(1)
Published: March 18, 2024
Abstract Water quality testing does not recognise antimicrobial resistance (AMR) and is often limited to indicators of faecal contamination Escherichia coli Enterococcus species. In Europe, data on AMR in drinking water scarce. Ireland, as many countries, household supplied via mains or private wells schemes. Using citizen science, we identified Irish supplies reservoirs resistant bacteria (ARB). Gram-negative ( n = 464) Gram-positive 72) were isolated. We instances potentially opportunistic ARB such Enterobacter cloacae, Acinetobacter baumannii report multidrug casseliflavus, E. coli, Stenotrophomonas maltophilia , Serratia rubidaea . also linezolid-resistant water. Linezolid a last-resort antibiotic used treat vancomycin-resistant sp. Additionally, mobile three samples, two which carried IncF group, one IncQ five Col-like plasmids. Our work suggests that potential sink source pathogens. This highlights value surveillance One Health framework the would provide information regarding movement persistence ARGs are able survive subsequently have opportunity be mobilised through humans; linking environment human threatening health.
Language: Английский
Citations
10
EBioMedicine, Journal Year: 2024, Volume and Issue: 103, P. 105097 - 105097
Published: April 11, 2024
Extended-spectrum cephalosporins (ESCs) are third and fourth generation cephalosporin antimicrobials used in humans animals to treat infections due multidrug-resistant (MDR) bacteria. Resistance ESCs (ESC-R) Enterobacterales is predominantly the production of extended-spectrum β-lactamases (ESBLs) plasmid-mediated AmpC (AmpCs). The dynamics ESBLs AmpCs changing across countries host species, result global transmission ESC-R genes. Plasmids known play a key role this dissemination, but relative importance different types plasmids not fully understood. In study, Escherichia coli with major genes blaCTX-M-1, blaCTX-M-15, blaCTX-M-14 blaCMY-2 (AmpC), were selected from diverse species other sources Canada, France Germany, collected between 2003 2017. To examine detail vehicles genes, long- short-read sequences generated obtain complete contiguous chromosome plasmid (n = 192 E. coli). types, gene composition genetic relatedness these investigated, along association isolate year, source geographical origin, put context publicly available sequences. We identified five epidemic resistance subtypes distinct properties that associated dissemination multiple lineages species. IncI1 pST3 blaCTX-M-1 subtype was found more than main subtypes, whereas pST12 frequent Canadian German human chicken isolates. Clonal expansion also contributed ST131 ST117 harbouring plasmid. pST2 predominant isolates France, while IncF F31:A4:B1 blaCTX-M-15 F2:A-:B- cattle countries. Beyond their nature respect our collection almost all carried antimicrobial (AMR) conferring classes. Finally, we signatures regions surrounding specific identifying mechanisms movement, using databases, widespread bacterial continents. provide evidence contribute addition, some confer success suggests they may have fitness advantage over subtypes. Identification understanding AMR crucial develop target strategies interventions reduce spread AMR. This project supported by Joint Programming Initiative on Antimicrobial (JPIAMR), through Medical Research Council (MRC, MR/R000948/1), Institutes Health (CFC-150770), Genomics Development (Government Canada), Federal Ministry Education (BMBF) grant no. 01KI1709, French Agency for food environmental occupational health & safety (Anses), National Reference Center (CNR) resistance. Support provided Biotechnology Biological Sciences (BBSRC) BBSRC Institute Strategic Programme Microbes Food ChainBB/R012504/1 its constituent BBS/E/F/000PR10348 (Theme 1, Epidemiology Evolution Pathogens Chain).
Language: Английский
Citations
6
Antibiotics, Journal Year: 2024, Volume and Issue: 13(4), P. 363 - 363
Published: April 16, 2024
Extended-spectrum β-lactamase-producing Escherichia coli ST131 has become widespread worldwide. This study aims to characterize the virulome, resistome, and population structure of E. isolates from clinical blood samples in Hungary. A total 30 C2/H30Rx 33 C1-M27 were selected for Illumina MiSeq sequencing MinION sequencing, followed by hybrid de novo assembly. Five one cluster identified. harbored F1:A2:B20 plasmid 93.9% cases. Long-read revealed that blaCTX-M-27 was on plasmids. Among isolates, only six carried C2-associated F2:A1:B- type. Of 19 hybrid-assembled genomes, blaCTX-M-15 gene located isolate, while other ISEcp1 or IS26-mediated chromosomal integration detected unique variations. In isolate a part integrated into chromosome. These results suggest CTX-M-15-producing CTX-M-27-producing subclades may have emerged spread different ways While mainly C1/H30R-associated plasmid, IncF-like plasmids its composite transposons been incorporated chromosome through convergent evolutionary processes.
Language: Английский
Citations
6
One Health, Journal Year: 2025, Volume and Issue: 20, P. 100968 - 100968
Published: Jan. 10, 2025
Diarrheal disease pathogens often spread through water-borne routes. Enterotoxigenic Escherichia coli (ETEC) is a major bacterial agent causing diarrheal in children, adults, and travelers endemic areas. In addition, ETEC responsible for outbreaks of water food-borne gasteroenteritis globally, isolates also show robust survival capacity various environmental settings, including aquatic environments. During the last decade, studies have indicated rapid increase multi-drug resistant extended-spectrum β-lactamase (ESBL)-positive human-specific strains. These been found both sources human patients, warranting urgent need focused monitoring antibiotic resistance development ETEC. Whole genome sequencing (WGS) from environmental, animal, enables silico surveillance emerging pathogenic This method allows re-analysis genomic data, aiding identification new variants clones. By integrating data diverse inclusing sequenced isolates, we that certain clonal lineages e.g., those expressing toxin-colonization factor profiles STp/CS6, LT STh/CS2 + CS3, STh/CFA/I are more at risk to develop than other lineages. Comparizon multi-locus sequence types papers with WGS ST182, ST4, ST2332 ST be We conclude further on ETEC/E. genomes needed enhance our understanding dynamics evolution, relation virulence clinical isolates.
Language: Английский
Citations
0
mBio, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 25, 2025
ABSTRACT Small regulatory RNAs (sRNAs) in bacteria are crucial for controlling various cellular functions and provide immediate response to the environmental stresses. Antibiotic persistence is a phenomenon that small subpopulation of survives under exposure lethal concentration antibiotics, potentially leading development drug resistance bacteria. Here, we reported novel transposon-derived sRNA called stnpA, which can modulate fosfomycin The stnpA located transposon with its own promoter highly conserved among prevalent multidrug (MDR) plasmids pathogenic expressed stress. It directly bind ABC transporter, YadG, whereas this protein–RNA interaction modulated export led enhancement bacterial persistence. According our knowledge, first identified sRNA, controlled antibiotic bacteria, work demonstrated nonresistance genes on MDR such as plasmid-encoded additional survival advantages host against antibiotics. In addition, be utilized druggable target therapeutic strategies overcome IMPORTANCE This study unveils groundbreaking discovery realm persistence, highlighting pivotal role newly RNA (sRNA) interacts transporter YadG efflux fosfomycin. Our findings elucidate mechanism RNA-regulated provides potential pathway emergence upon treatment. Importantly, example linking regulation presenting target. underscores critical noncoding adaptation offers valuable insights developing new combat
Language: Английский
Citations
0
Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(5), P. 418 - 418
Published: April 28, 2025
tet(X4)-positive IncHI1 plasmids are widely prevalent in various bacteria. To understand their transmission characteristics, we analyzed two extensively drug-resistant (XDR) Escherichia coli strains isolated from pet dog feces Henan Province, China. Strain T28R harbored IncHI1, IncF18:A-:B-, and mcr-1-positive IncI2 plasmids, while T16R carried F16:A-:B-, IncX4 plasmids. Four representative fusion pT28R-F1, pT28R-F2, pT28R-F3, pT16R-F1, transconjugants were using WGS PCR mapping. The results showed that IS26 the IncF18:A-:B--plasmid attacked conjugative transfer-associated genes trhc rsp on plasmid, generating pT28R-F1 pT28R-F2. pT28R-F3 was generated through ISCro1- ISCR2-mediated homologous recombination, deleting Tra1 region of plasmid. T16R-F1 emerged ISCR2- IS1B-mediated losing transfer regions parental Notably, lost temperature sensitivity with conjugation frequencies between 1.57 × 10−4 3.84 10−5 at 28 °C 37 °C. findings suggest could be mobilized assistance helper events enhance adaptability these thus facilitating spread antibiotic resistance, posing a growing public health threat.
Language: Английский
Citations
0
Frontiers in Bioscience-Landmark, Journal Year: 2025, Volume and Issue: 30(4)
Published: April 24, 2025
Background: Escherichia coli (E. coli) is a common opportunistic bacterial pathogen in both human and animal populations. Fatty acids serve as the central carbon energy source, process mediated by fatty acid-coenzyme A (CoA) ligases encoded fad genes such FadK. However, function mechanism of FadK remain unclear. Methods: The three-dimensional structure was modeled using AlphaFold2. After expression purification, monomeric successfully isolated. enzymatic activity assayed, real-time quantitative polymerase chain reaction (RT-qPCR) performed to quantify levels. Results: In assays acid CoA ligase activity, caprylic found be optimal substrate for We determined catalytic conditions FadK, which include pH 7.4, ATP concentration 0.6 mM, 0.8 Mg2+ mM at 37 °C. Notably, showed decrease with increasing concentrations dodecyl-AMP, further confirmed RT-qPCR results. Conclusions: Our findings will fundamental framework development innovative therapeutics that target E. infections.
Language: Английский
Citations
0