Serum and Seminal Plasma Zinc Levels and Immunopositivity of the ZIP6 and ZIP14 Transporters in Men with Normo- and Teratozoospermia

Current Issues in Molecular Biology, Год журнала: 2025, Номер 47(2), С. 101 - 101

Опубликована: Фев. 6, 2025

Zinc plays a crucial role in spermatogenesis, sperm function, and fertilisation. homeostasis is regulated by ZIP ZnT transporter proteins, which mediate Zn2+ influx efflux across cell membranes. This study analysed total Zn concentration seminal plasma serum of 10 normozoospermic 32 teratozoospermic men involved the process infertility treatment, using inductively coupled mass spectrometry. In addition, expression transporters ZIP6 ZIP14 two was immunofluorescence. Applying Student’s t test Mann–Whitney U test, we found no significant differences concentrations between groups. mainly localised head, with slightly higher immunopositivity than samples, but there statistically difference some samples showed tail, although were samples. The results suggest that serum, ZIP14, do not differ normo- teratozospermic emphasise complex interplay factors underlying male fertility.

Язык: Английский

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APHRODITE criteria: Addressing male patients with hypogonadism and/or infertility owing to altered idiopathic testicular function

Reproductive BioMedicine Online, Год журнала: 2023, Номер 48(4), С. 103647 - 103647

Опубликована: Окт. 29, 2023

Can a novel classification system of the infertile male - 'APHRODITE' (Addressing Patients with Hypogonadism and/or infeRtility Owing to altereD, Idiopathic TEsticular function) stratify different subgroups infertility help scientists design clinical trials on hormonal treatment infertility, and clinicians counsel treat endocrinological imbalances in men and, ultimately, increase chances natural assisted conception?

Язык: Английский

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Diagnosis and management of infertility: NICE-adapted guidelines from the Italian Society of Human Reproduction

Reproductive Biology and Endocrinology, Год журнала: 2024, Номер 22(1)

Опубликована: Янв. 5, 2024

Abstract In Italy the fertility rate is very low, and an increasing number of patients are infertile require treatments. The Italian Law concerning safety patient care, professional liability health professionals, indicates that professionals must comply with recommendations set out in guidelines developed by public private bodies institutions, as well scientific societies technical-scientific associations professions, except for specific cases. Unfortunately, no guideline diagnosis management infertility currently available Italy. 2019, Society Human Reproduction pointed need to produce subsequently approved establishment a multidisciplinary multiprofessional working group (MMWG) develop such guideline. MMWG was representative 5 societies, one national federation orders, 3 citizens' patients' associations, professions (including lawyer, biologist, doctor, midwife, psychologist), medical specialties genetics, obstetrics gynecology, urology). chose adapt high-quality context instead developing from scratch. Using version Appraisal Guidelines Research Evaluation II scoring system, National Institute Clinical Excellence were selected adapted context. document improved upon incorporating comments suggestions where needed. This study presents process adaptation discusses pros cons often-neglected choice adapting rather than new guidelines.

Язык: Английский

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Cancer risk and male Infertility: Unravelling predictive biomarkers and prognostic indicators

Clinica Chimica Acta, Год журнала: 2024, Номер 558, С. 119670 - 119670

Опубликована: Апрель 16, 2024

Язык: Английский

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Advanced Sperm Selection Techniques for Assisted Reproduction

Journal of Personalized Medicine, Год журнала: 2024, Номер 14(7), С. 726 - 726

Опубликована: Июль 4, 2024

Male infertility accounts for approximately 40% of cases. There are many causes male infertility, including environmental factors, age, lifestyle, infections, varicocele, and cancerous pathologies. Severe oligozoospermia, cryptozoospermia, azoospermia (obstructive non-obstructive) identified as severe factor once considered conditions sterility. Today, in vitro fertilization (IVF) techniques the only treatment strategy cases which new methodologies have been developed manipulation spermatozoa to achieve increase success rates. This review is an update techniques, particular sperm selection, emphasizing clinical case-specific methodology. The IVF process related diagnosis, appropriate choice treatment, effective preparation selection. In fact, selecting best guarantee optimal paternal heritage means increasing blastulation, implantation, ongoing pregnancy live birth rates, resulting greater techniques.

Язык: Английский

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A Comparative Analysis of Gradient Centrifugation and Microfluidic Chips for Sperm Selection

IntechOpen eBooks, Год журнала: 2025, Номер unknown

Опубликована: Янв. 4, 2025

A variety of techniques have been employed in the field sperm selection, including methods based on density, morphological characteristics, motility membrane integrity, and surface charge. Among these techniques, density gradient centrifugation (DGC) method stands out as most prevalent widely utilized today. However, this technique involves steps that linked to oxidative stress subsequent cellular damage. Consequently, alternatives DGC are being continuously developed. The recently developed microfluidic chip has also implemented for selection intracytoplasmic injection (ICSI). presents certain disadvantages must be addressed. This chapter evaluates advantages from past present, with a particular focus devices.

Язык: Английский

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First clinical pregnancy and delivery achieved after using a new 3D imaging technology for sperm selection: a case report

Frontiers in Reproductive Health, Год журнала: 2025, Номер 7

Опубликована: Март 7, 2025

Objective To report a case of patient who, after experiencing recurrent failed implantations, underwent novel technological intervention—using the Q300 device—which successfully led to pregnancy and delivery healthy baby. Design Case report. Setting Barzilai University Medical Center. Patient (s) A 33-year-old woman with primary infertility experienced implantation failure (RIF), while her 32-year-old male partner was diagnosed severe oligo-astheno-teratozoospermia (OTA) syndrome. Intervention Using device for selection morphologically compliant sperm cells intracytoplasmic injection (ICSI). Main outcome measures Successful delivery. Results unique clinical involving couple facing RIF OTA. In this case, new technology used. The semen sample examined using choose WHO2021-morphologically micro-injection. resulting embryos were developed then frozen. Later, frozen-thawed embryo transfer performed during following natural menstrual cycle, leading successful Conclusion utilization 3D imaging underscores evolving landscape reproductive medicine potential it holds transforming outcomes in challenging cases. By documenting such cases, we contribute ongoing dialogue refine assisted (ART) protocols improve individuals similar challenges. Trial registration NCT06232720 https://clinicaltrials.gov/study/NCT06232720 . Date registration: 15 Feb 2023. enrollment first subject: 20 August

Язык: Английский

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Temporal Trends in the Burden of Disease for Male Infertility from 1990 to 2021 in the BRICS

Risk Management and Healthcare Policy, Год журнала: 2025, Номер Volume 18, С. 1721 - 1733

Опубликована: Май 1, 2025

Язык: Английский

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Environmental and Genetic Traffic in the Journey from Sperm to Offspring

Biomolecules, Год журнала: 2023, Номер 13(12), С. 1759 - 1759

Опубликована: Дек. 7, 2023

Recent advancements in the understanding of how sperm develop into offspring have shown complex interactions between environmental influences and genetic factors. The past decade, marked by a research surge, has not only highlighted profound impact paternal contributions on fertility reproductive outcomes but also revolutionized our comprehension unveiling parental factors sculpt traits successive generations through mechanisms that extend beyond traditional inheritance patterns. Studies are more susceptible to factors, especially during critical phases growth. While these broadly detrimental health, their effects acute periods. Moving immutable nature genome, epigenetic profile cells emerges as dynamic architecture. This flexibility renders it disruptions. primary objective this review is shed light diverse processes which agents affect male capacity. Additionally, explores consequences interactions, demonstrating can reverberate offspring. It encompasses direct changes well broad spectrum adaptations. By consolidating current empirically supported research, offers an exhaustive perspective interwoven trajectories environment, genetics, epigenetics elaborate transition from

Язык: Английский

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Sperm YOLOv8E-TrackEVD: A Novel Approach for Sperm Detection and Tracking

Sensors, Год журнала: 2024, Номер 24(11), С. 3493 - 3493

Опубликована: Май 28, 2024

Male infertility is a global health issue, with 40–50% attributed to sperm abnormalities. The subjectivity and irreproducibility of existing detection methods pose challenges assessment, making the design automated semen analysis algorithms crucial for enhancing reliability evaluations. This paper proposes comprehensive tracking algorithm (Sperm YOLOv8E-TrackEVD) that combines an enhanced YOLOv8 small object (SpermYOLOv8-E) improved DeepOCSORT (SpermTrack-EVD) detect human in microscopic field view track healthy sample short period effectively. Firstly, we trained model on VISEM-Tracking dataset accurate detection. To enhance objects, introduced attention mechanism, added layer, integrated SPDConv Detect_DyHead modules. Furthermore, used new distance metric method chose IoU loss calculation. Ultimately, achieved 1.3% increase precision, 1.4% recall rate, 2.0% improvement [email protected]:0.95. We applied SpermYOLOv8-E combined SpermTrack-EVD tracking. On dataset, 74.303% HOTA 71.167% MOTA. These results show effectiveness designed Sperm YOLOv8E-TrackEVD approach scenarios.

Язык: Английский

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