Microbiology, Год журнала: 2025, Номер 94(1), С. 116 - 127
Опубликована: Фев. 1, 2025
Язык: Английский
Biomass Conversion and Biorefinery, Год журнала: 2023, Номер 14(20), С. 25395 - 25409
Опубликована: Июль 20, 2023
Abstract Fusarium species are considered one of the most destructing plant pathogens. In current study, bimetallic zinc oxide-copper oxide nanoparticles (ZnO-CuO NPs) were myco-synthesized using Aspergillus fumigatus for controlling oxysporum growth. was isolated from soil and identified morphologically genetically. The ZnO-CuO NPs characterized UV-Vis, DLS, HR-TEM, SEM, XRD analyses. HR-TEM characterization method indicated that, biosynthesized appeared as semi-spherical with average diameter specified 54.18 ± 1.9 nm. DLS described characteristic particle size diffusion calculated 85.52 nm, 90.85 92.85 nm ZnO NPs, CuO respectively. Additionally, SEM image displays basic NP surface character exterior impression apparent. separated naturally spherical particles connected within fungal filtrate, which illuminated fused capped filtrate. Antifungal activity evaluated against F. . Results revealed that exhibited promising antifungal toward where inhibition zone at 1000 µg/ml 22.8 0.76 mm, MIC 125 µg/ml. Moreover, growth percentages different concentrations 1000, 500, 250, 88.9, 65.5, 41.1, 8.9% respectively, highest 88.9% concentration µg/ml, while lowest TEM ultrastructure results, treated a clear destruction found in all cell contents disintegration wall well plasma membrane. Also, nucleus small damaged shape chromatin materials distributed several dark stained bodies cytoplasm. conclusion, successfully A. , it had
Язык: Английский
Процитировано
41
International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(15), С. 12227 - 12227
Опубликована: Июль 31, 2023
Biological plant protection presents a promising and exciting alternative to chemical methods for safeguarding plants against the increasing threats posed by diseases. This approach revolves around utilization of biological control agents (BCAs) suppress activity significant pathogens. Microbial BCAs have potential effectively manage crop disease development interacting with pathogens or hosts, thereby their resistance. However, current efficacy remains unsatisfactory, creating new research opportunities sustainable cultivation management. In this context, microbial consortia, comprising multiple microorganisms diverse mechanisms action, hold promise in terms augmenting magnitude stability overall antipathogen effect. Despite scientific efforts identify construct consortia that can aid vital crops, only limited number consortia-based biocontrol formulations are currently available. Therefore, article aims present complex analysis status explore future directions technological advancements.
Язык: Английский
Процитировано
34
Archives of Microbiology, Год журнала: 2023, Номер 205(2)
Опубликована: Янв. 21, 2023
Язык: Английский
Процитировано
27
Microbial Pathogenesis, Год журнала: 2023, Номер 180, С. 106131 - 106131
Опубликована: Апрель 28, 2023
Язык: Английский
Процитировано
25
Biomass Conversion and Biorefinery, Год журнала: 2023, Номер 14(18), С. 23073 - 23089
Опубликована: Июль 7, 2023
Abstract Crop loss brought on by phytopathogens is a significant issue that affects the entire world. Synthetic pesticides have been used for years to prevent economic losses caused phytopathogens, raising severe worries about environment and public health. Therefore, today, there growing interest in using helpful microorganisms control plant pathogens. In this way, Aspergillus chevalieri AGH-1 OP593083 egyptiacus AGH-4 OP593080 isolated from wheat rhizosphere applied as growth-promoting fungi (PGPF) combating early blight disease Alternaria solani ( A. ) inducing defense system growth promotional strategies Vicia faba . Two milligrams per milliliter ethyl acetate crude extracts of demonstrated effective antifungal efficacy against with an inhibition zone 18 16 mm respectively compared 20 attained fluconazole positive control. Moreover, minimum inhibitory concentrations (MIC) were (0.25 0.5 mg/ml) respectively. Data showed was best isolate producing indole acetic acid (IAA) siderophores (+ + +) followed +). Also, isolates capable phosphate solubilization soil +), It interesting PGPF used, whether single or mixed, improvement all yield well photosynthetic pigments played important role improving immunity increasing phenol, proline, antioxidant enzymes (POD PPO) activities, reducing oxidative stress MDA H 2 O We can conclude are considered eco-friendly safe strong inducers alternatives chemical preserve plant, soil, human
Язык: Английский
Процитировано
24
Biomass Conversion and Biorefinery, Год журнала: 2023, Номер 14(16), С. 19613 - 19622
Опубликована: Фев. 28, 2023
Abstract The search for active inducers against diseases in the formula of therapeutic nutrients has become a necessity many researchers. study’s chief purpose was to make agronomic farming simpler by applying newly created nutrients. novelty this research is applied algal extracts adding minerals as nutrion. Calcium (Maxifos Ca), Ascophyllum nodosum (Greencal), and Arthrospira platensis ( A. ), were tested induction pepper plant resistance Fusarium wilt. disease index (DI), morphological growth, photosynthetic pigments, free proline, total phenol, hydrogen peroxide (H 2 O malondialdehyde (MDA), antioxidant enzymes reactions protection challenged plants measured. Results revealed that use entirely different treatments significantly minimized danger Treatment infected with Maxifos Ca best treatment, it reduced DI 25% thus symptoms improved percentage from 69.6%. Surprisingly, widely assumed Greencal greatest treatment restoring vegetative followed an extract, . application Greencal, Ca, then increased expression all metabolic indices (phenols, polyphenol oxidase, peroxidase). reducing signs stress represented (MDA H ) Greencal. According findings alternate eco-destructive chemically synthesized fungicides appears be significant methodology harmful effects wilt on plants.
Язык: Английский
Процитировано
23
Microbiological Research, Год журнала: 2024, Номер 283, С. 127678 - 127678
Опубликована: Март 6, 2024
Charcoal rot caused by Macrophomina phaseolina is one of the most devastating diseases that cause severe yield loss in Gloriosa superba cultivation. Plant growth-promoting rhizobacteria (PGPR) are extensively harnessed as biocontrol agents due to their effectiveness combating a wide array plant pathogens through multifaceted approach. The present study delved into mechanisms underlying its ability inhibit root pathogen and capacity promote growth G. superba, commonly known glory lily. PGPR isolated from rhizosphere lily were subjected vitro assessments using dual plate technique. Bacillus subtilis BGS-10 B. velezensis BGS-21 showed higher mycelial inhibition (61%) against M. phaseolina. These strains also producing indole-3-acetic acid, siderophore, ammonia, amylase, cellulase, pectinase, xylanase, lipase chemicals. Genome screening revealed presence antimicrobial peptide genes such Iturin (ituD gene), surfactin (srfA sfp genes) along with mycolytic enzyme β-1,3-glucanase. Further, secondary metabolites bacterial secretome was identified gas chromatography-mass spectrometry (GC/MS) analysis. Notably, pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl), 9 H-pyrido[3,4-b] indole L-leucyl-D-leucine exhibited highest docking score enzymes responsible for cell wall degradation. Under glasshouse conditions, tuber treatment soil application talc-based formulation suppress incidence minimal disease 27.78% over untreated control. Concurrently, there notable induction defense-related enzymes, including peroxidase (PO), polyphenol oxidase (PPO), phenylalanine ammonia-lyase (PAL), Therefore, it can be concluded play significant role fortifying plant's defense pathogen.
Язык: Английский
Процитировано
12
Frontiers in Plant Science, Год журнала: 2024, Номер 15
Опубликована: Фев. 6, 2024
Agricultural productivity in the Great Lakes Countries of Central Africa, including Burundi, Rwanda, and Democratic Republic Congo, is affected by a wide range diseases pests which are mainly controlled chemical pesticides. However, more than 30% pesticides used region banned European Union due to their high toxicity. Globally available safe eco-friendly biological alternatives chemicals virtually non-existent region. Bacillus PGPR-based biocontrol products most dominant market have proven efficacy controlling major plant reported With this review, we present current situation disease pest management urge need utilize -based control as possible sustainable alternative A repertoire strains from subtilis group that shown great potential antagonize local pathogens provided, efforts promote use, well search for indigenous adapted agro-ecological conditions, should be undertaken make agriculture reality
Язык: Английский
Процитировано
11
Frontiers in Microbiology, Год журнала: 2024, Номер 15
Опубликована: Фев. 5, 2024
Introduction The rhizosphere microbiome is critical to plant health and resistance. PGPR are well known as plant-beneficial bacteria generally regulate nutrient utilization responses environmental stimuli. In our previous work, one typical strain, Pseudomonas chlororaphis IRHB3, isolated from the soybean rhizosphere, had positive impacts on soil-borne disease suppression growth promotion in greenhouse, but its biocontrol mechanism application field not unclear. Methods current study, IRHB3 was introduced into soil, effects local microbiome, resistance, were comprehensively analyzed through high-throughput sequencing physiological molecular methods. Results discussion We found that significantly increased richness of bacterial community structure rhizosphere. Functional related phosphorus solubilization nitrogen fixation, such Geobacter , Geomonas Candidatus Solibacter Occallatibacter Koribacter recruited rich abundance by compared those without IRHB3. addition, supplement obviously maintained homeostasis disturbed F. oxysporum resulting a lower index root rot when with . Furthermore, JA-mediated induced resistance rapidly activated following PDF1.2 LOX2 expression, meanwhile, set nodulation genes, GmENOD40b GmNIN-2b GmRIC1 also considerably improve fixation ability promote yield, even plants infected Thus, tends synergistically interact microbes host induce field.
Язык: Английский
Процитировано
10
Physiological and Molecular Plant Pathology, Год журнала: 2024, Номер 133, С. 102337 - 102337
Опубликована: Июнь 12, 2024
Язык: Английский
Процитировано
10