Exogenous dsRNA triggers sequence-specific RNAi and fungal stress responses to control Magnaporthe oryzae in Brachypodium distachyon

Communications Biology, Год журнала: 2025, Номер 8(1)

Опубликована: Янв. 25, 2025

In vertebrates and plants, dsRNA plays crucial roles as PAMP a mediator of RNAi. How higher fungi respond to is not known. We demonstrate that Magnaporthe oryzae (Mo), globally significant crop pathogen, internalizes across broad size range 21 about 3000 bp. Incubation fungal conidia with 10 ng/µL dsRNA, regardless or sequence, induced aberrant germ tube elongation, revealing strong sequence-unspecific effect in this fungus. Accordingly, the synthetic analogue poly(I:C) various sizes sequences elicited canonical stress pathways, including nuclear accumulation marker mitogen-activated protein kinase Hog1p production ROS. Leaf application cereal model species Brachypodium distachyon suppressed progression leaf blast disease. Notably, depends on doses, while pure sequence-specific effects were observed at low concentrations ( < 0.03 ng/µL). The protective further enhanced by maintaining gap least seven days between inoculation, stabilising alginate-chitosan nanoparticles. Overall, our study opens up additional possibilities for development use pesticides agriculture.

Язык: Английский

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A Review on Biocontrol Agents as Sustainable Approach for Crop Disease Management: Applications, Production, and Future Perspectives

Horticulturae, Год журнала: 2024, Номер 10(8), С. 805 - 805

Опубликована: Июль 30, 2024

Horticultural crops are vulnerable to diverse microbial infections, which have a detrimental impact on their growth, fruit quality, and productivity. Currently, chemical pesticides widely employed manage diseases in horticultural crops, but they negative effects the environment, human health, soil physiochemical properties, biodiversity. Additionally, use of has facilitated development spread resistant pathovars, emerged as serious concern contemporary agriculture. Nonetheless, adverse consequences environment public health worried scientists greatly recent years, led switch biocontrol agents such bacteria, fungi, insects control plant pathogens. Biocontrol (BCAs) form an integral part organic farming, is regarded future sustainable Hence, harnessing potential BCAs important viable strategy disease way that also ecofriendly can improve health. Here, we discuss role biological crops. We different microbial-based fungal, bacterial, viral management. Next, factors affect performance under field conditions. This review highlights genetic engineering enhance efficiency other growth traits. Finally, highlight challenges opportunities biocontrol-based management horticulture research directions boost efficacy applications.

Язык: Английский

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Lysozyme-coated LDHs boost trait control

Nature Plants, Год журнала: 2025, Номер unknown

Опубликована: Янв. 2, 2025

Язык: Английский

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Unravelling the secondary metabolome and biocontrol potential of the recently described species Bacillus nakamurai

Microbiological Research, Год журнала: 2024, Номер 288, С. 127841 - 127841

Опубликована: Авг. 2, 2024

In the prospect of novel potential biocontrol agents, a new strain BDI-IS1 belonging to recently described Bacillus nakamurai was selected for its strong in vitro antimicrobial activities against range bacterial and fungal phytopathogens. Genome mining coupled with metabolomics revealed that produces multiple non-ribosomal secondary metabolites including surfactin, iturin A, bacillaene, bacillibactin bacilysin, together some ribosomally-synthesized post-translationally modified peptides (RiPPs) such as plantazolicin, potentially amylocyclicin, bacinapeptin LCI. Reverse genetics further showed specific involvement these compounds antagonistic activity strain. Comparative genomics between five already sequenced B. strains products constitute core metabolome species while RiPPs are more strain-specific. Although lacks key bioactive found velezensis, greenhouse experiments show is able protect tomato maize plants early blight northern leaf caused by Alternaria solani Exserohilum turcicum, respectively, at levels similar or better than velezensis QST713. The reduction foliar diseases, following root application suspension demonstrates can act direct antibiosis inducing plant defence mechanisms. These findings indicate be considered good candidate diseases prevailing tropical regions, encourage research into spectrum activity, requirements conditions needed ensure efficacy.

Язык: Английский

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Optimizing the production of dsRNA biocontrols in microbial systems using multiple transcriptional terminators

Biotechnology and Bioengineering, Год журнала: 2024, Номер 121(11), С. 3582 - 3599

Опубликована: Июль 18, 2024

Abstract Crop pests and pathogens annually cause over $220 billion in global crop damage, with insects consuming 5%–20% of major grain crops. Current pest disease control strategies rely on insecticidal fungicidal sprays, plant genetic resistance, transgenes, agricultural practices. Double‐stranded RNA (dsRNA) is emerging as a novel sustainable method protection an alternative to traditional chemical pesticides. Successful commercialization dsRNA‐based biocontrols requires the economical production large quantities dsRNA combined suitable delivery methods ensure RNAi efficacy against target pest. In this study, we have optimized design plasmid DNA constructs produce Escherichia coli , by employing wide range synthetic transcriptional terminators before measurement yield. We demonstrate that 7.8‐fold increase was achieved using triple within dual T7 system compared absence terminators. Moreover, our data batch fermentation multiple scalable generates significantly higher yields generated at both small‐scale culture large‐scale fermentation. addition, show application these expressed E. cells results increased insect mortality. Finally, mass spectrometry analysis performed determine precise sites termination different providing important further mechanistic insight.

Язык: Английский

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Evaluation of the Control Efficacy of Bt Maize Expressing Cry1Ab and Vip3Aa Proteins Against Agrotis ypsilon (Rottemberg)

Insects, Год журнала: 2025, Номер 16(2), С. 119 - 119

Опубликована: Янв. 26, 2025

Bt maize is the main means to control many lepidopteran pests in world, but its efficacy against Agrotis ypsilon (Rottemberg), an important insect pest of seedlings, remains unclear until now. The interaction between and transgenic events (DBN9936 (expressing Cry1Ab), DBN9501 Vip3Aa), DBN3601T Cry1Ab Vip3Aa)) was investigated using bioassay behavioral tests. results show that contents different tissues DBN9936 were 47.78–82.60 μg·g−1, Vip3Aa 15.29–27.78 μg·g−1. 32.08–79.08 10.16–17.52 respectively. There no significant difference total protein content leaves stems; however, both significantly higher than roots. larvae most sensitive protein, corrected mortalities feeding on greater 89.65% at seedling stage, those (16.46–76.13%). third fifth instar root as follows: (54.00–96.60%) > (24.67–70.88%) (6.67–53.31%). tests for Bt/non-Bt plant selection indicated mainly fed non-Bt while showing antifeedant behavior toward maize, moth preferred lay eggs undamaged or slightly damaged maize. It concluded has a strong A. ypsilon, which can play role building integrated management strategy insect.

Язык: Английский

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The utilization of biological control against regulated pests in the EPPO region: challenges and opportunities

EPPO Bulletin, Год журнала: 2025, Номер unknown

Опубликована: Фев. 4, 2025

Abstract Biological control is a pest method that can offer an environmentally safer alternative to chemical pesticides. The proven safety record of both augmentative and classical biological technologies allows its utilization against indigenous non‐indigenous but well‐established pests, whether under protected conditions (e.g., glasshouses) or in open field cropping systems. This manuscript has been developed by the Joint European Mediterranean Plant Protection Organization (EPPO) International for Integrated Control (IOBC) Panel on Agents presents assessment current use regulated plant pests. paper discusses challenges uptake pests provides recommendations increase safe agents EPPO region.

Язык: Английский

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Influence of temperature and maize genotypes on the population dynamics of Sitophilus zeamais Motschulsky 1885 (COLEOPTERA: CURCULIONIDAE) and grain quality during storage

Journal of Stored Products Research, Год журнала: 2025, Номер 111, С. 102564 - 102564

Опубликована: Фев. 6, 2025

Язык: Английский

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Bio-based strategies for biotic and abiotic stress management in sustainable agriculture in EU

Biocontrol Science and Technology, Год журнала: 2025, Номер unknown, С. 1 - 17

Опубликована: Фев. 12, 2025

Язык: Английский

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New entomopathogenic species in the Clavicipitaceae family (Hypocreales, Ascomycota) from the subtropical forests of Fujian, China

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Март 3, 2025

Introduction Entomopathogenic fungi play a crucial role in the ecological regulation of insect populations and can be exploited as resource for pest control, sustainable agriculture, natural products discovery. These their infected hosts are sometimes highly coveted part traditional medicine practices. Here, we sought to examine biodiversity entomogenous subtropical forests China. Methods Fungal-infected specimens were collected from various sites Fujian Province, China, purified isolates obtained through laboratory cultivation isolation techniques. Molecular characterization specific target genomic loci was performed on fungal isolates, used phylogenetic analyses using Bayesian inference maximum likelihood methods elucidate taxonomic relationships. Microscopy describe morphological features isolates. Results Through comprehensive two-year survey Province via multilocus molecular analysis targeting nr SSU , LSU tef1-α rpb1, rpb2 specimens, identified three novel species within Clavicipitaceae herein described as: Albacillium fuzhouense sp. nov., Conoideocrella gongyashanensis nov. Neoaraneomyces wuyishanensis well recently recorded, Metarhizium cicadae . Each new also distinguished its closest relatives by unique characteristics. Discussion discoveries enrich our understanding family contribute development control strategies

Язык: Английский

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