Exogenous dsRNA triggers sequence-specific RNAi and fungal stress responses to control Magnaporthe oryzae in Brachypodium distachyon

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: Jan. 25, 2025

In vertebrates and plants, dsRNA plays crucial roles as PAMP a mediator of RNAi. How higher fungi respond to is not known. We demonstrate that Magnaporthe oryzae (Mo), globally significant crop pathogen, internalizes across broad size range 21 about 3000 bp. Incubation fungal conidia with 10 ng/µL dsRNA, regardless or sequence, induced aberrant germ tube elongation, revealing strong sequence-unspecific effect in this fungus. Accordingly, the synthetic analogue poly(I:C) various sizes sequences elicited canonical stress pathways, including nuclear accumulation marker mitogen-activated protein kinase Hog1p production ROS. Leaf application cereal model species Brachypodium distachyon suppressed progression leaf blast disease. Notably, depends on doses, while pure sequence-specific effects were observed at low concentrations ( < 0.03 ng/µL). The protective further enhanced by maintaining gap least seven days between inoculation, stabilising alginate-chitosan nanoparticles. Overall, our study opens up additional possibilities for development use pesticides agriculture.

Language: Английский

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A Review on Biocontrol Agents as Sustainable Approach for Crop Disease Management: Applications, Production, and Future Perspectives

Horticulturae, Journal Year: 2024, Volume and Issue: 10(8), P. 805 - 805

Published: July 30, 2024

Horticultural crops are vulnerable to diverse microbial infections, which have a detrimental impact on their growth, fruit quality, and productivity. Currently, chemical pesticides widely employed manage diseases in horticultural crops, but they negative effects the environment, human health, soil physiochemical properties, biodiversity. Additionally, use of has facilitated development spread resistant pathovars, emerged as serious concern contemporary agriculture. Nonetheless, adverse consequences environment public health worried scientists greatly recent years, led switch biocontrol agents such bacteria, fungi, insects control plant pathogens. Biocontrol (BCAs) form an integral part organic farming, is regarded future sustainable Hence, harnessing potential BCAs important viable strategy disease way that also ecofriendly can improve health. Here, we discuss role biological crops. We different microbial-based fungal, bacterial, viral management. Next, factors affect performance under field conditions. This review highlights genetic engineering enhance efficiency other growth traits. Finally, highlight challenges opportunities biocontrol-based management horticulture research directions boost efficacy applications.

Language: Английский

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Lysozyme-coated LDHs boost trait control

Nature Plants, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

Language: Английский

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Unravelling the secondary metabolome and biocontrol potential of the recently described species Bacillus nakamurai

Microbiological Research, Journal Year: 2024, Volume and Issue: 288, P. 127841 - 127841

Published: Aug. 2, 2024

In the prospect of novel potential biocontrol agents, a new strain BDI-IS1 belonging to recently described Bacillus nakamurai was selected for its strong in vitro antimicrobial activities against range bacterial and fungal phytopathogens. Genome mining coupled with metabolomics revealed that produces multiple non-ribosomal secondary metabolites including surfactin, iturin A, bacillaene, bacillibactin bacilysin, together some ribosomally-synthesized post-translationally modified peptides (RiPPs) such as plantazolicin, potentially amylocyclicin, bacinapeptin LCI. Reverse genetics further showed specific involvement these compounds antagonistic activity strain. Comparative genomics between five already sequenced B. strains products constitute core metabolome species while RiPPs are more strain-specific. Although lacks key bioactive found velezensis, greenhouse experiments show is able protect tomato maize plants early blight northern leaf caused by Alternaria solani Exserohilum turcicum, respectively, at levels similar or better than velezensis QST713. The reduction foliar diseases, following root application suspension demonstrates can act direct antibiosis inducing plant defence mechanisms. These findings indicate be considered good candidate diseases prevailing tropical regions, encourage research into spectrum activity, requirements conditions needed ensure efficacy.

Language: Английский

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Optimizing the production of dsRNA biocontrols in microbial systems using multiple transcriptional terminators

Biotechnology and Bioengineering, Journal Year: 2024, Volume and Issue: 121(11), P. 3582 - 3599

Published: July 18, 2024

Abstract Crop pests and pathogens annually cause over $220 billion in global crop damage, with insects consuming 5%–20% of major grain crops. Current pest disease control strategies rely on insecticidal fungicidal sprays, plant genetic resistance, transgenes, agricultural practices. Double‐stranded RNA (dsRNA) is emerging as a novel sustainable method protection an alternative to traditional chemical pesticides. Successful commercialization dsRNA‐based biocontrols requires the economical production large quantities dsRNA combined suitable delivery methods ensure RNAi efficacy against target pest. In this study, we have optimized design plasmid DNA constructs produce Escherichia coli , by employing wide range synthetic transcriptional terminators before measurement yield. We demonstrate that 7.8‐fold increase was achieved using triple within dual T7 system compared absence terminators. Moreover, our data batch fermentation multiple scalable generates significantly higher yields generated at both small‐scale culture large‐scale fermentation. addition, show application these expressed E. cells results increased insect mortality. Finally, mass spectrometry analysis performed determine precise sites termination different providing important further mechanistic insight.

Language: Английский

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BioSolutions for Green Agriculture: Unveiling the Diverse Roles of Plant Growth‐Promoting Rhizobacteria

International Journal of Microbiology, Journal Year: 2024, Volume and Issue: 2024(1)

Published: Jan. 1, 2024

The extensive use of chemical pesticides and fertilizers in conventional agriculture has raised significant environmental health issues, including the emergence resistant pests pathogens. Plant growth-promoting rhizobacteria (PGPR) present a sustainable alternative, offering dual benefits as biofertilizers biocontrol agents. This review delves into mechanisms by which PGPR enhance plant growth, nutrient solubilization, phytohormone production, pathogen suppression. PGPR's commercial viability application, particularly under abiotic stress conditions, are also examined. improves growth directly enhancing uptake producing substances indirectly inhibiting phytopathogens through such siderophore production secretion lytic enzymes. Despite their potential, commercialization faces challenges, strain specificity, formulation stability, regulatory barriers. highlights need for ongoing research to deepen our understanding plant-microbe interactions develop more robust formulations. Addressing these challenges will be crucial integrating mainstream agricultural practices reducing reliance on synthetic agrochemicals. successful adoption could lead practices, promoting healthier crops ecosystems.

Language: Английский

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Designer antisense circRNAGFP reduces GFP abundance in Arabidopsis protoplasts in a sequence-specific manner, independent of RNAi pathways

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Circular RNAs (circRNAs) are single-stranded RNA molecules characterised by their covalently closed structure and emerging as key regulators of cellular processes in mammals, including gene expression, protein function immune responses. Recent evidence suggests that circRNAs also play significant roles plants, influencing development, nutrition, biotic stress resistance, abiotic tolerance. However, the potential to modulate target abundance plants remains largely unexplored. In this study, we investigated designer using Arabidopsis a model system. We demonstrate treatment with 50 nt circRNA_GFP, containing 30 GFP antisense sequence stretch, results reduced reporter dose- sequence-dependent manner. Notably, open isoform circRNA_GFP had little effect on abundance, indicating importance circular structure. Additionally, mutants defective interference (RNAi), suggesting circRNA activity is independent RNAi pathway. show circRNA, unlike dsRNA, does not induce pattern-triggered immunity (PTI) plants. Findings proof-of-principle study together crucial first steps understanding versatile tools for modulating expression offer exciting prospects application agronomy, particularly enhancing crop traits through metabolic pathway manipulation.

Language: Английский

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Piroplasm infestations in cattle: exploring tick control using Chrysanthemum extract and neem oil emulsion

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: March 31, 2025

Tick-borne diseases represent a major threat to both animal and human health globally. This study explores the prevalence of tick infestation associated piroplasm infections specifically Theileria Babesia species in cattle, addition evaluating acaricidal effectiveness Chrysanthemum extract (Dendranthema grandiflora) neem oil emulsion (Azadirachta indica). Among 130 cattle examined, 61 were infested with ticks subsequently screened for infections. Molecular analysis identified caused by annulata bigemina. A strong association was found between species, while T. infection showed slight correlation. Hemolymph examination confirmed critical role life cycle infection. tested their properties against adult (Rhipicephalus annulatus). (0.5 mg/mL) mortality within 24 h. However, induced rapid significant at (20 mg/L) (15 mg/L), achieving 100% same time frame. Both treatments demonstrated high effectiveness, results indicating dose-and time-dependent effects compared controls. Scanning electron microscopy (SEM) revealed extensive morphological damage treated ticks. included destruction hypostome, loss surface striations, wrinkling pore formation, cracking following exposure extract. These findings highlight potential D. grandiflora as effective natural acaricides controlling infestations reducing tick-borne diseases.

Language: Английский

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Biological control agents: the importance for specific and targeted screening techniques to enable their effective and successful implementation

European Journal of Plant Pathology, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Language: Английский

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Evaluation of the Control Efficacy of Bt Maize Expressing Cry1Ab and Vip3Aa Proteins Against Agrotis ypsilon (Rottemberg)

Insects, Journal Year: 2025, Volume and Issue: 16(2), P. 119 - 119

Published: Jan. 26, 2025

Bt maize is the main means to control many lepidopteran pests in world, but its efficacy against Agrotis ypsilon (Rottemberg), an important insect pest of seedlings, remains unclear until now. The interaction between and transgenic events (DBN9936 (expressing Cry1Ab), DBN9501 Vip3Aa), DBN3601T Cry1Ab Vip3Aa)) was investigated using bioassay behavioral tests. results show that contents different tissues DBN9936 were 47.78–82.60 μg·g−1, Vip3Aa 15.29–27.78 μg·g−1. 32.08–79.08 10.16–17.52 respectively. There no significant difference total protein content leaves stems; however, both significantly higher than roots. larvae most sensitive protein, corrected mortalities feeding on greater 89.65% at seedling stage, those (16.46–76.13%). third fifth instar root as follows: (54.00–96.60%) > (24.67–70.88%) (6.67–53.31%). tests for Bt/non-Bt plant selection indicated mainly fed non-Bt while showing antifeedant behavior toward maize, moth preferred lay eggs undamaged or slightly damaged maize. It concluded has a strong A. ypsilon, which can play role building integrated management strategy insect.

Language: Английский

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