Antimicrobial activity, induction of ROS generation in HepG2 liver cancer cells, and chemical composition of Pterospermum heterophyllum DOI Creative Commons

Tu Q,

Lê Thị Hương,

Mai Quynh Nguyen

и другие.

Open Chemistry, Год журнала: 2024, Номер 22(1)

Опубликована: Янв. 1, 2024

Abstract The objective of this investigation was to assess the inhibitory effects extracts derived from Pterospermum heterophyllum on bacteria and fungi, as well their capacity impede proliferation migration HepG2 liver cancer cells. Extracts leaves stems P. revealed ability resist all three bacterial strains, including Escherichia coli , Pseudomonas aeruginosa Streptococcus aureus ( p < 0.05). Additionally, also inhibited growth fungal strains: Aspergillus brasiliensis Candida albicans flavus . Evaluation anticancer activity showed that significantly reduced cells, with IC 50 values for leaf stem being 35.5 47.17 µg/mL, respectively. Remarkably, extract herbal species cells after 24 h treatment. Fluorescence analysis using 2-HDCFDA a considerable elevation in reactive oxygen production within treated extracts, which is hypothesized contribute reduction cell viability. Subsequent chemical compound utilizing GC-FID spectra identified some main compounds present This study highlights potential antimicrobial activities

Язык: Английский

A Review of the Biotechnological Potential of Cave Fungi: A Toolbox for the Future DOI Creative Commons
Renan do Nascimento Barbosa, Maria Tamara de Caldas Felipe,

Leticia Francisca Da Silva

и другие.

Journal of Fungi, Год журнала: 2025, Номер 11(2), С. 145 - 145

Опубликована: Фев. 14, 2025

The study of the intersection between biodiversity and biotechnology has revealed a rich source innovations. Fungi, with their vast range morphologies lifestyles, thrive in various habitats, including caves. With impressive metabolic characteristics, they play key role producing essential biotechnological compounds for economic sectors. This paper aims to consolidate evidence on potential fungi isolated from caves, highlighting urgency conserving exploring these ecosystems. For this purpose, we conducted comprehensive literature search using scientific databases (SciELO, Medline Complete, Medline/PubMed, Web Science, Scopus (Elsevier), Google Scholar). We adopted an interdisciplinary approach by collecting information 22 papers published 2013 2024. Based data, our survey broad potential, antimicrobial compounds, antioxidants, antitumor agents, enzymes, organic acids. emphasize that accurately identifying depositing fungal isolates reference collections are crucial reliable research effective industrial applications, driving bioactivity production substances inhibit pathogens. Conserving protecting cave environment is imperative, considering its continuous discovery contribution advancement.

Язык: Английский

Процитировано

1

Research on the metabolites and key metabolic enzymes of allocryptopine in chicken liver microsomes via stable isotope tracing technology DOI
Zhiyong Wu, Xinhao Wang, Lin Wang

и другие.

Journal of Pharmaceutical and Biomedical Analysis, Год журнала: 2025, Номер 255, С. 116667 - 116667

Опубликована: Янв. 6, 2025

Язык: Английский

Процитировано

0

Naringenin-Loaded Solid Lipid Nanoparticles: Physical–Chemical Characterization and In Vitro Antibacterial Activity DOI Creative Commons
Federica De Gaetano, F. Caridi,

Noemi Totaro

и другие.

Pharmaceuticals, Год журнала: 2025, Номер 18(2), С. 232 - 232

Опубликована: Фев. 8, 2025

Currently, problems related to antibiotic resistance are shifting the focus of pharmaceutical research towards natural molecules with antibacterial properties. Among them, flavonoids represent promising strong features; however, they have poor biopharmaceutical In this study, we developed solid lipid nanoparticles (SLNs) loaded flavanone naringenin (NRG) offer an option for treating bacterial infections. NRG-SLNs systems were prepared by a solvent emulsification/diffusion and ultrasonication method, using Compritol® 888 ATO (COM) as lipid. The optimal formulation was obtained 10% (w/w) theoretical amount NRG (NRG10-SLNs), exhibiting homogeneous sizes (approximately 50 nm 0.15 polydispersity index), negative zeta potential (-30 mV), excellent encapsulation parameters (an efficiency percentage 97.9% drug content 4%). NRG10-SLNs presented good physical stability over 4 weeks. A cumulative release 55% in 24 h prolonged remaining 10 days observed. addition, µ-Raman spectroscopy, differential scanning calorimetry, thermogravimetric analysis, X-ray diffraction measurements carried out characterize drug-lipid interactions. Finally, vitro antibiofilm activities assayed compared free NRG. bacteriostatic against Staphylococcus aureus, including methicillin-resistant S. aureus (MRSA) Escherichia coli strains. An improvement activity NRG-loaded SLNs molecule observed strains, probably due interaction surfactant-coated surface. similar trend biofilm inhibition.

Язык: Английский

Процитировано

0

Comprehensive Overview of Antibacterial Drugs and Natural Antibacterial Compounds Found in Food Plants DOI Creative Commons
Sabine Berteina‐Raboin

Antibiotics, Год журнала: 2025, Номер 14(2), С. 185 - 185

Опубликована: Фев. 11, 2025

The aim of this review is to list the various natural sources antimicrobials that are readily available. Indeed, many plant known have antibiotic properties, although it not always clear which molecule responsible for activity. Many food supplements also therapeutic indication. We propose here take stock scientific knowledge attesting or these indications some sources. An overview drugs commercially available will be provided. A structural indication molecules present in plants and reported contribute their power given. mentioned review, does claim exhaustive, referenced fighting Gram-positive and/or Gram-negative bacteria. It difficult attribute activity just one molecules, as likely result from synergy within plant. Similarly, chitosan its fungistatic bacteriostatic properties. In case, polymeric compound derived chitin marine organisms antibiofilm seems that, face growing resistance, makes sense keep high-performance synthetic antibiotics on hand treat pathologies require them. On other hand, minor infections, use better-tolerated certainly sufficient. To achieve this, we need common sources, products dietary supplements, active field.

Язык: Английский

Процитировано

0

Anti-biofilm and anti-quorum sensing activities of galloylquinic acid against clinical isolates of multidrug-resistant Pseudomonas aeruginosa in open wound infection: in vitro and in vivo efficacy studies DOI Creative Commons

Lamiaa A Al-Maddboly,

Mohamed Abd El-Salam,

Jairo K. Bastos

и другие.

BMC Microbiology, Год журнала: 2025, Номер 25(1)

Опубликована: Апрель 9, 2025

Pseudomonas aeruginosa can proliferate in immunocompromised individuals, forming biofilms that increase antibiotic resistance. This bacterium poses a significant global health risk due to its resistance human defenses, antibiotics, and various environmental stresses. The objective of this study was evaluate the antibacterial, anti-biofilm, anti-quorum sensing activities galloylquinic acid compounds (GQAs) extracted from Copaifera lucens leaves against clinical isolates multidrug-resistant (MDR) P. aeruginosa. We have investigated optimal concentration GQAs needed eradicate preexisting manage wound infections caused by aeruginosa, vitro vivo. Our results revealed exhibited 25-40 mm inhibition zone diameters, with 1-4 µg/mL MIC 2-16 MBC values. interfered planktonic mode isolates, significantly inhibited their growth pre-formed biofilm architecture, MBIC80 MBEC80 values 64 128 µg/mL, respectively. anti-biofilm effect confirmed fluorescence staining confocal microscopy which showed dramatic reduction cell viability thickness (62.5%), after exposure particular. scanning electron micrographs impaired bacterial structures interfering biomass exopolysaccharides matrix. also virulence factors motility, where (p < 0.05) reduced rhamnolipid, pyocyanin, swarming motility organism play vital role formation. downregulated 89% quorum-sensing genes (lasI lasR, pqsA pqsR) involved demonstrate promise as novel potent antibiofilm antivirulence agents MDR substantial potential enhance healing biofilm-associated infections. promising antibacterial action positions superior alternative for treatment infections, improve mitigate impact persistent not applicable.

Язык: Английский

Процитировано

0

Bioinspired Nanomaterials to Combat Microbial Biofilm and Pathogen Challenges: A Review DOI
Atefeh Zarepour, Meenakshi Venkateswaran, Arezoo Khosravi

и другие.

ACS Applied Nano Materials, Год журнала: 2024, Номер 7(22), С. 25287 - 25313

Опубликована: Ноя. 12, 2024

The emergence of antibiotic-resistant biofilms poses a significant challenge in healthcare, as these complex microbial communities demonstrate an increased resistance to conventional treatment methods. Traditional antibiotics often fail against biofilms, resulting persistent infections and failures. To address this urgent issue, innovative strategies such bioinspired nanomaterials, antimicrobial peptides, quorum sensing inhibitors, combination therapies show promise disrupting biofilm structures, enhancing activity, overcoming mechanisms. Bioinspired nanomaterials have emerged pivotal approach for tackling the challenges presented by pathogens across various sectors, including industry, environmental protection. Their advantages include enhanced biocompatibility, targeted delivery, improved efficacy formation threats. Recent advancements highlight potential solutions, nanoparticles, smart nanocarriers, surface modifications, nanozymes, combating biofilm-related issues. Despite progress nanomaterial research, remain. intricate interactions within evolving nature necessitate multidisciplinary approaches. Furthermore, translating laboratory findings into practical applications faces obstacles related scalability, stability, regulatory compliance. Future are expected focus on multifunctional nanoparticles that disrupt advanced modifications better interaction, nanocarriers nanozymes dismantle structures. This review focuses development application pathogen challenges. It emphasizes roles targeting capabilities. Additionally, explores formulating management practices, providing insights advantages, limitations, future perspectives

Язык: Английский

Процитировано

3

Lipid Nanoparticle Formulations for the Skin Delivery of Cannabidiol DOI Creative Commons
María Natalia Calienni, Mariangela Scavone,

Ana Paula Sanguinetti

и другие.

Pharmaceutics, Год журнала: 2024, Номер 16(12), С. 1490 - 1490

Опубликована: Ноя. 21, 2024

Background/Objectives: The aims of this work were to formulate cannabidiol in different lipid carriers for skin delivery after topical application and study their stability, interaction with the skin, antibacterial activity. Methods: Solid nanoparticles nanostructured loaded prepared characterized terms physicochemical properties, colloidal protection antioxidant capacity cannabidiol, as well retention over time. Skin penetration was assessed using an vitro model human skin. activity tested against Staphylococcus aureus compared free cannabidiol. Results: Three nanoformulations exhibited best size reproducibility values selected further studies. formulations stable, protected active ingredient, succeeded delivering it deep layers, demonstrated Conclusions: These show potential use diseases conditions, they protect enhance its exhibit effects.

Язык: Английский

Процитировано

0

Antimicrobial activity, induction of ROS generation in HepG2 liver cancer cells, and chemical composition of Pterospermum heterophyllum DOI Creative Commons

Tu Q,

Lê Thị Hương,

Mai Quynh Nguyen

и другие.

Open Chemistry, Год журнала: 2024, Номер 22(1)

Опубликована: Янв. 1, 2024

Abstract The objective of this investigation was to assess the inhibitory effects extracts derived from Pterospermum heterophyllum on bacteria and fungi, as well their capacity impede proliferation migration HepG2 liver cancer cells. Extracts leaves stems P. revealed ability resist all three bacterial strains, including Escherichia coli , Pseudomonas aeruginosa Streptococcus aureus ( p < 0.05). Additionally, also inhibited growth fungal strains: Aspergillus brasiliensis Candida albicans flavus . Evaluation anticancer activity showed that significantly reduced cells, with IC 50 values for leaf stem being 35.5 47.17 µg/mL, respectively. Remarkably, extract herbal species cells after 24 h treatment. Fluorescence analysis using 2-HDCFDA a considerable elevation in reactive oxygen production within treated extracts, which is hypothesized contribute reduction cell viability. Subsequent chemical compound utilizing GC-FID spectra identified some main compounds present This study highlights potential antimicrobial activities

Язык: Английский

Процитировано

0