The Controversial Roles of Areca Nut: Medicine or Toxin?
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(10), С. 8996 - 8996
Опубликована: Май 19, 2023
Areca
nut
(AN)
is
used
for
traditional
herbal
medicine
and
social
activities
in
several
countries.
It
was
as
early
about
A.D.
25-220
a
remedy.
Traditionally,
AN
applied
medicinal
functions.
However,
it
also
reported
to
have
toxicological
effects.
In
this
review
article,
we
updated
recent
trends
of
research
addition
acquire
new
knowledge
AN.
First,
the
history
usage
from
ancient
years
described.
Then,
chemical
components
their
biological
functions
compared;
arecoline
an
especially
important
compound
extract
has
different
effects
caused
by
components.
Thus,
dual
with
pharmacological
were
summarized.
Finally,
described
perspectives,
challenges
will
provide
insight
removing
or
modifying
toxic
compounds
extractions
enhancing
activity
treat
diseases
future
applications.
Язык: Английский
The Yin and Yang of TLR4 in COVID-19
Cytokine & Growth Factor Reviews,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 1, 2024
Язык: Английский
Exploring the diverse biological activities of Garcinia cowa: Implications for future cancer chemotherapy and beyond
Food Bioscience,
Год журнала:
2024,
Номер
61, С. 104525 - 104525
Опубликована: Июнь 6, 2024
Язык: Английский
Spike Protein of SARS-CoV-2 Activates Cardiac Fibrogenesis through NLRP3 Inflammasomes and NF-κB Signaling
Cells,
Год журнала:
2024,
Номер
13(16), С. 1331 - 1331
Опубликована: Авг. 11, 2024
Background:
The
spike
protein
of
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
is
crucial
to
viral
entry
and
can
cause
cardiac
injuries.
Toll-like
receptor
4
(TLR4)
NOD-,
LPR-,
pyrin-domain-containing
3
(NLRP3)
inflammasome
are
critical
immune
system
components
implicated
in
fibrosis.
activates
NLRP3
through
TLR4
or
angiotensin-converting
enzyme
(ACE2)
receptors,
damaging
various
organs.
However,
the
role
fibrosis
humans,
as
well
its
interactions
with
inflammasomes
TLR4,
remain
poorly
understood.
Methods:
We
utilized
scratch
assays,
Western
blotting,
immunofluorescence
evaluate
migration,
signaling,
mitochondrial
calcium
levels,
reactive
oxygen
species
(ROS)
production,
cell
morphology
cultured
human
fibroblasts
(CFs)
treated
(S1)
for
24
h
without
an
anti-ACE2
neutralizing
antibody,
a
blocker,
inhibitor.
Results:
S1
enhanced
CFs
migration
expressions
collagen
1,
α-smooth
muscle
actin,
transforming
growth
factor
β1
(TGF-β1),
phosphorylated
SMAD2/3,
interleukin
1β
(IL-1β),
nuclear
kappa-light-chain-enhancer
activated
B
cells
(NF-κB).
increased
ROS
production
but
did
not
affect
content
morphology.
Treatment
antibody
attenuated
effects
on
1
TGF-β1
expressions.
Moreover,
(MCC950)
NF-kB
inhibitors,
inhibitor
TAK-242,
prevented
protein-enhanced
overexpression
TGF-β1,
IL-1β.
Conclusion:
by
priming
NF-κB
signaling
ACE2-dependent
manner.
Язык: Английский
SARS-CoV-2 spike receptor-binding domain is internalized and promotes protein ISGylation in human induced pluripotent stem cell-derived cardiomyocytes
Scientific Reports,
Год журнала:
2023,
Номер
13(1)
Опубликована: Дек. 4, 2023
Although
an
increased
risk
of
myocarditis
has
been
observed
after
vaccination
with
mRNA
encoding
severe
acute
respiratory
syndrome
coronavirus
2
spike
protein,
its
underlying
mechanism
not
elucidated.
This
study
investigated
the
direct
effects
receptor-binding
domain
(S-RBD)
on
human
cardiomyocytes
differentiated
from
induced
pluripotent
stem
cells
(iPSC-CMs).
Immunostaining
experiments
using
ACE2
wild-type
(WT)
and
knockout
(KO)
iPSC-CMs
treated
purified
S-RBD
demonstrated
that
was
bound
to
internalized
into
subcellular
space
in
iPSC-CMs,
depending
ACE2.
combined
live
cell
imaging
a
recombinant
fused
superfolder
GFP
(S-RBD-sfGFP)
membrane,
co-localized
RAB5A,
then
delivered
endosomes
lysosomes
iPSC-CMs.
Quantitative
PCR
array
analysis
followed
by
single
RNA
sequence
clarified
S-RBD-sfGFP
treatment
significantly
upregulated
NF-kβ
pathway-related
gene
(CXCL1)
non-cardiomyocytes,
while
interferon
(IFN)-responsive
genes
(IFI6,
ISG15,
IFITM3)
matured
cardiomyocytes.
promoted
protein
ISGylation,
ISG15-mediated
post-translational
modification
ACE2-WT-iPSC-CMs,
which
suppressed
ACE2-KO-iPSC-CMs.
Our
experimental
demonstrates
is
through
endolysosomal
pathway,
upregulates
IFN-responsive
promotes
ISGylation
Язык: Английский