TerC proteins function during protein secretion to metalate exoenzymes

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Окт. 4, 2023

Cytosolic metalloenzymes acquire metals from buffered intracellular pools. How exported are appropriately metalated is less clear. We provide evidence that TerC family proteins function in metalation of enzymes during export through the general secretion (Sec-dependent) pathway. Bacillus subtilis strains lacking MeeF(YceF) and MeeY(YkoY) have a reduced capacity for protein greatly level manganese (Mn) secreted proteome. MeeF MeeY copurify with secretory pathway, their absence FtsH membrane protease essential viability. also required efficient Mn

Язык: Английский

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Fluorescent Protein-Based Sensors for Detecting Essential Metal Ions across the Tree of Life

ACS Sensors, Год журнала: 2024, Номер 9(4), С. 1622 - 1643

Опубликована: Апрель 8, 2024

Genetically encoded fluorescent metal ion sensors are powerful tools for elucidating dynamics in living systems. Over the last 25 years since first examples of genetically protein-based calcium indicators, this toolbox probes has expanded to include other essential and non-essential ions. Collectively, these have illuminated fundamental aspects homeostasis trafficking that crucial fields ranging from neurobiology human nutrition. Despite advances, much application remains limited mammalian cells tissues a number metals. Applications beyond systems vivo applications organisms primarily used sensors. The aim Perspective is provide, with support historical recent literature, an updated critical view design use detecting ions various organisms. We highlight progress achievements discuss more advances opportunities detection also outstanding challenges field directions future studies, including wider variety ions, developing implementing broader spectral range multiplexing experiments, applying single- multi-species biological

Язык: Английский

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Effects of Sewage Sludge Compost on Carbon, Nitrogen, Phosphorus, and Sulfur Ratios and Soil Enzyme Activities in a Long-Term Experiment

Agronomy, Год журнала: 2025, Номер 15(1), С. 143 - 143

Опубликована: Янв. 9, 2025

The carbon, nitrogen, phosphorus, and sulfur (CNPS) ratios of soils are known to be relatively stable parameters, characterizing different land uses. We hypothesized that the long-term application sewage sludge compost (SSC) would not change these but increase concentration elements quality organic matter (OM), as well soil enzyme activities. Hence, chemical microbiological properties were studied in a 20-year experiment. plots grouped into five blocks treated every third year with SSC at rates 0, 9, 18, or 27 t ha−1. Three plants, crop rotation, tested sown follows: rye, rye hairy vetch, maize. results showed basic parameters (pH, OM content, E4/E6 ratio, NO3-NO2-N, AL-P2O5, moisture content) increased, along doses for rye. Similar trends found CNPS concentrations, β-glucosidase, alkaline phosphatase activities, while acidic activity was reduced. C:N, C:S, N:S affected by application. main factors treatment effects plant-available phosphorus (ammonium lactate (AL)-soluble P2O5), total P, based on principal component analysis. canonical correspondent analyses revealed activities C:P, N:P β-glucosidase correlated P forms pH strongly all enzymes. Based role microbes became more important increasing mobilization. conclude addition could improve health through pH, OM, nutrient microbial activity. Also, some elemental have an regulation

Язык: Английский

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A Microbial-Centric View of Mobile Phones: Enhancing the Technological Feasibility of Biotechnological Recovery of Critical Metals

Bioengineering, Год журнала: 2025, Номер 12(2), С. 101 - 101

Опубликована: Янв. 22, 2025

End-of-life (EoL) mobile phones represent a valuable reservoir of critical raw materials at higher concentrations compared to primary ores. This review emphasizes the need transition from single-material recovery approaches comprehensive, holistic strategies for recycling EoL phones. In response call sustainable techniques with reduced energy consumption and pollutant emissions, biohydrometallurgy emerges as promising solution. The present work intends most relevant studies focusing on exploitation microbial consortia in bioleaching biorecovery processes. All living organisms macro- micronutrients their metabolic functionalities, including some elements contained By exploring interactions between communities diverse found phones, this paper establishes microbial-centric perspective by connecting each element layer role cell system. A special focus is dedicated concepts ecodesign modularity key requirements electronics potentially increase selectivity process. bridging science design, proposes an innovative roadmap optimize metal recovery, aligning principles circular economy advancing scalable biotechnological solutions electronic waste management.

Язык: Английский

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Nitrogen source orchestrates pH modulation and secondary metabolism in Trichoderma harzianum

Chemical and Biological Technologies in Agriculture, Год журнала: 2025, Номер 12(1)

Опубликована: Фев. 4, 2025

Язык: Английский

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The search for ancient life on Mars using morphological and mass spectrometric analysis: an analog study in detecting microfossils in Messinian gypsum

Frontiers in Astronomy and Space Sciences, Год журнала: 2025, Номер 12

Опубликована: Фев. 25, 2025

Hydrated sulfate deposits have been detected on Mars. A spaceflight instrument capable of detecting microfossils in these salt is highly important for the search ancient life This study employed a range analytical methods, including nondestructive optical microscopy and SEM-EDX, as well spatially resolved laser ablation mass spectrometry (LIMS), latter being designed in-situ analyses planetary surfaces, to comprehensively examine morphology, texture, mineralogy, geochemistry fossil-bearing gypsum from Algeria. These extensive formations formed during Messinian Salinity Crisis (MSC) serve excellent astrobiological analogs large-scale hydrated Significant research reveals notable microbial fossil filaments. aims determine whether LIMS measurements together can detect filaments samples, identify their composition, decipher biogenicity syngeneity. Spatially depth profiling chemical mapping analysis one representative filament using provided detailed mineralogical compositional variations that correlate with distinctive morphological features. findings collectively indicate exhibits distinct composition diagenetic processes comparison surrounding host. The microfossil’s syngeneity were established based presence biosignatures, biologically relevant elements, induced or influenced minerals such dolomite clay minerals. formation within physico-chemical context Martian lakes was also discussed. same suite techniques could be applied microfossil-bearing Mars beyond.

Язык: Английский

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Origin of the RNA World in Cold Hadean Geothermal Fields Enriched in Zinc and Potassium: Abiogenesis as a Positive Fallout from the Moon-Forming Impact?

Life, Год журнала: 2025, Номер 15(3), С. 399 - 399

Опубликована: Март 4, 2025

The ubiquitous, evolutionarily oldest RNAs and proteins exclusively use rather rare zinc as transition metal cofactor potassium alkali cofactor, which implies their abundance in the habitats of first organisms. Intriguingly, lunar rocks contain a hundred times less ten than Earth’s crust; Moon is also depleted other moderately volatile elements (MVEs). Current theories impact formation attribute this depletion to MVEs still being gaseous state when hot post-impact disk contracted separated from nascent Moon. then fell out onto juvenile protocrust; zinc, most metal, precipitated last, just after potassium. According our calculations, top layer protocrust must have contained up 1019 kg metallic powerful reductant. venting geothermal fluids through MVE-fallout layer, rich radioactive potassium, both capable reducing carbon dioxide dinitrogen, yielded plethora organic molecules released with vapor. In pools vapor condensate, RNA-like may emerged pre-Darwinian selection for low-volatile, associative, mineral-affine, radiation-resistant, nitrogen-rich, polymerizable molecules.

Язык: Английский

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Heavy Metal Health Risk Assessment in Picea abies L. Forests Along an Altitudinal Gradient in Southern Romania

Plants, Год журнала: 2025, Номер 14(6), С. 968 - 968

Опубликована: Март 19, 2025

Heavy metals (HMs) from industrial pollution are bioaccumulated in plant tissues, but we lack information on their spatial variability forest ecosystems. Picea abies L. needles, bark, and litter were collected at 17 sites along a 1000-m-wide altitudinal gradient southern Romania to measure concentrations of mineral nutrients, non-toxic metals, toxic metals. Isotopic ratios (206/207Pb, 87/86Sr) used infer contamination origins. We found significant differences needle versus bark elements’ concentrations, indicating that needles sensitive biomarkers tracking air pollution. high Fe, Mn, Zn, Cu which can be involved the low Na, Mg, P content needles. The mole indicate negative relationship with As Several environmental health ecological risk assessment indices document Cd levels represent moderate for most sites. Our study shows P. presented an elevated bioaccumulation potential Cu, Ni, Cr, respectively, it only absorbed Sr, Cd, Li. methodology results herein may serve as reference future studies provide foundation develop management strategies mitigate heavy metal

Язык: Английский

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Sustained and comparative habitability beyond Earth

Nature Astronomy, Год журнала: 2023, Номер 8(1), С. 30 - 38

Опубликована: Дек. 28, 2023

Язык: Английский

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Toxicity of rare earth elements (REEs) to marine organisms: Using species sensitivity distributions to establish water quality guidelines for protecting marine life

Environmental Research, Год журнала: 2024, Номер 261, С. 119708 - 119708

Опубликована: Июль 30, 2024

Язык: Английский

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