iPSC‐derived human cortical organoids display profound alterations of cellular homeostasis following SARS‐CoV‐2 infection and Spike protein exposure
The FASEB Journal,
Год журнала:
2025,
Номер
39(4)
Опубликована: Фев. 14, 2025
Abstract
COVID‐19
commonly
leads
to
respiratory
issues,
yet
numerous
patients
also
exhibit
a
diverse
range
of
neurological
conditions,
suggesting
detrimental
impact
SARS‐CoV‐2
or
the
viral
Spike
protein
on
central
nervous
system.
Nonetheless,
molecular
pathway
behind
pathology
and
presumed
neurotropism
remains
largely
unexplored.
We
generated
human
cortical
organoids
(HCOs)
derived
from
induced
pluripotent
stem
cells
(hiPSC)
assess:
(1)
expression
main
entry
factors;
(2)
their
vulnerability
infection;
(3)
infection
exposure
transcriptome.
Results
proved
that
HCOs
express
receptors
co‐receptors;
may
be
productively
infected
by
SARS‐CoV‐2;
particles
released
SARS‐CoV‐2‐infected
are
able
re‐infect
another
cellular
line;
(4)
resulted
in
activation
apoptotic
stress
pathways,
along
with
inflammatory
processes.
Notably,
these
effects
were
recapitulated
when
exposed
alone.
The
data
obtained
demonstrate
likely
infects
probably
through
binding
ACE2,
CD147,
NRP1
factors.
Furthermore,
alone
sufficient
disrupt
homeostasis
induce
neurotoxic
effects,
potentially
contributing
onset
long‐COVID
symptoms.
Язык: Английский
Clinically Significant Functional Impairments and Symptoms in COVID‐19 Survivors: Empirical Research Quantitative
Journal of Clinical Nursing,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 14, 2025
ABSTRACT
Background
COVID‐19
survivors
may
experience
complex,
distressing
and
persistent
symptoms,
referred
to
as
long
COVID,
lasting
months
or
years
after
diagnosis.
More
evidence
is
needed
for
effective
COVID
screening
management.
Aim
To
explore
the
clinical
profile
of
factors
associated
with
its
development.
Design
A
multicentre
correlational
study
using
a
cross‐sectional
design.
Methods
Adults
diagnosed
6–9
earlier
were
recruited
via
social
media
referrals
from
three
facilities.
Participants
provided
demographic
data
assessed
their
symptoms
functional
status
validated
questionnaires.
Data
analysed
descriptive
statistics
binomial
logistic
regression.
Results
Among
102
participants,
13%–30%
reported
significant
impairments
in
cognitive,
emotional
physical
functioning.
Over
10%
experienced
such
diarrhoea,
sleep
problems,
dyspnoea,
nausea,
fatigue
pain.
These
acute
symptom
severity,
chronic
disease,
overweight
status,
regular
exercise
living
without
partners.
Conclusion
This
adds
literature
by
examining
long‐term
omicron
comprehensive,
tools.
The
findings
highlight
prevalence
significance
aiding
identification
requiring
medical
nursing
interventions.
Implications
Profession
Patient
Care
Nurses
should
recognise
these
educate
about
potential
challenges.
Policies
addressing
issues,
including
research,
health
services
education,
are
essential.
Reporting
Method
follows
STROBE
guideline
(Table
S1).
Public
Contribution
Patients
self‐reported
this
study.
Trial
Registration
ClinicalTrials.gov
(NCT05303103)
Язык: Английский
Mast Cells and Microbiome in Health and Disease
Frontiers in Bioscience-Landmark,
Год журнала:
2025,
Номер
30(3)
Опубликована: Март 20, 2025
Inter-kingdom
communication
between
human
microbiota
and
mast
cells
(MCs),
as
sentinels
of
innate
immunity,
is
crucial
in
determining
health
disease.
This
complex
signaling
hub
involves
micro-organisms
and,
more
importantly,
their
metabolic
products.
Gut
the
host’s
largest
symbiotic
ecosystem
under
physiological
conditions,
it
plays
a
vital
role
mediating
MCs
tolerogenic
priming,
thus
ensuring
immune
homeostasis
across
organs.
Conversely,
intestinal
dysbiosis
various
etiologies
promotes
MC-oriented
inflammation
along
major
body
axes,
including
gut-skin,
gut-lung,
gut-liver,
gut-brain.
review
international
scientific
literature
provides
comprehensive
overview
cross-talk
investigation.
process
key
biological
event
involved
disease
development
clinical
fields,
with
significant
prognostic
therapeutic
implications
for
future
research.
Язык: Английский
The Antiviral Effect of Ephedrine Alkaloids-Free Ephedra Herb Extract, EFE, on Murine Coronavirus Growth in the Lung and Liver of Infected Mice
Microorganisms,
Год журнала:
2025,
Номер
13(4), С. 830 - 830
Опубликована: Апрель 6, 2025
Ephedrine
alkaloids-free
Ephedra
Herb
extract
(EFE)
was
developed
to
reduce
the
adverse
effects
of
Herb,
a
constituent
drug
in
Kampo
medicines.
It
is
produced
by
decocting
with
hot
water
and
excluding
ephedrine
alkaloids.
EFE
has
analgesic
anti-cancer
inhibits
respiratory
viruses
vitro.
To
assess
pharmacological
action
vivo,
we
evaluated
its
effect
on
replication
murine
hepatitis
virus
(MHV),
coronavirus
that
causes
hepatitis,
pneumonia,
severe
acute
syndrome-like
symptoms,
within
infected
mice.
On
Day
0,
MHV
inoculated
intranasally
into
female
BALB/C
mice,
orally
administered
once/day
at
350–700
mg/kg
(n
=
10/group)
starting
1
h
after
inoculation
until
5.
Through
plaque
assay,
detected
5
lung
liver
all
but
titer
significantly
lower
groups
as
compared
untreated
control
Although
not
statistically
significant,
clinical
score
for
irregularity
tended
be
treatment
groups.
In
conclusion,
an
vivo
mouse
model
human
infection
exerts
liver.
Язык: Английский
Could We Designate Long COVID a Syndrome?
Science Insights,
Год журнала:
2024,
Номер
44(6), С. 1389 - 1391
Опубликована: Июнь 29, 2024
Long
COVID
has
been
subject
to
growing
examination
as
researchers
and
healthcare
professionals
strive
gain
a
deeper
comprehension
of
its
fundamental
mechanisms
consequences.
While
syndromes
often
consist
group
symptoms
without
distinct
underlying
cause,
seems
be
more
intricate.
Studies
indicate
that
it
could
affect
several
organ
systems
cause
cellular-level
harm,
prompting
specialists
propose
categorized
syndrome
rather
than
simple
lingering
consequences
the
virus.
However,
further
study
needs
conducted
for
determining
whether
there
are
distinctive
characteristics
differentiate
clinical
entity
from
other
post-viral
conditions.
Accurate
identification
will
essential
in
guiding
treatment
approaches
delivering
appropriate
therapy
patients
who
suffer
persistent
after
being
infected
with
COVID-19.
Язык: Английский
Nerve Growth Factor and Brain-Derived Neurotrophic Factor in COVID-19
Biology,
Год журнала:
2024,
Номер
13(11), С. 907 - 907
Опубликована: Ноя. 7, 2024
Neurotrophins
(NTs)
constitute
a
family
of
small
protein
messengers
that
play
fundamental
role
in
both
the
central
and
peripheral
nervous
systems.
In
particular,
nerve
growth
factor
(NGF)
brain-derived
neurotrophic
(BDNF)
subtle
survival,
differentiation,
functioning
neuronal
populations,
as
well
fine
regulation
immune
functions.
The
SARS-CoV-2
infection
was
characterized
by
sequela
symptoms
(serious
respiratory
pathology,
inflammatory
storm,
neurological
discomfort,
up
to
less
serious
flu-like
symptoms),
which
caused,
at
end
2023,
more
than
7
million
deaths
worldwide.
Despite
official
pandemic,
physical
psychological
consequences
are
currently
object
scientific
research,
acute
chronic/long-lasting
(Long-COVID-19).
Given
multifactorial
nature
outcomes
adults
children,
several
studies
have
investigated
potential
involvement
NGF
BDNF
systems
pathology.
This
narrative
review
aims
summarize
most
recent
evidence
on
this
crucial
topic.
Язык: Английский
Novel Advancements in COVID-19 and Neuroscience
Journal of Personalized Medicine,
Год журнала:
2024,
Номер
14(2), С. 143 - 143
Опубликована: Янв. 28, 2024
From
an
initial
cluster
of
cases
reported
in
Wuhan,
the
SARS-Cov-2
infection
has
since
spread
globally,
causing
a
pandemic
that
began
on
11
March
2020
[...]
Язык: Английский
The Effects of Six Weeks Pulmonary Rehabilitation on Functional and Psychological Outcomes in Long-COVID Patients: Preliminary Results from Serbian Single Center Study
Medicina,
Год журнала:
2024,
Номер
60(4), С. 671 - 671
Опубликована: Апрель 21, 2024
Background
and
Objectives:
In
this
study,
we
aimed
to
evaluate
the
effects
of
six
weeks
pulmonary
rehabilitation
on
functional
psychological
outcomes
in
long-COVID
patients.
Material
Methods:
The
prospective
clinical
study
included
46
patients
that
were
diagnosed
with
COVID-19.
A
respiratory
program
was
implemented
for
weeks.
Further
valuables
tested
before
beginning
(admission)
after
(discharge):
SpO2,
heart
rate,
Visual
Analogue
Scale
(VAS)
score,
Borg
Sit-to-Stand
(StS)
test
number
repetition,
distance
6-Minute
Walking
Test
(6MWT),
Patient
Health
Questionnaire
(PHQ)
9
score
Generalized
anxiety
disorder
(GAD)
score.
These
parameters
admission
at
discharge
discharge.
results
presented
standard
descriptive
analytical
methods.
Differences
between
continuous
variables
physical
intervention
using
Wilcoxon
test.
Graphical
analysis
is
a
box
plot.
Results:
On
discharge,
comparison
admission,
values
SpO2
significantly
lower
(p
=
0.007)
6MWT,
VAS
scores
higher
0.036),
while
<
0.001)
as
0.016).
rate
0.005)
StS
test,
0.001),
levels
0.036)
0.008).
After
0.011),
PHQ9
GAD
well
0.005),
distances
measured
meters
6MWT
increased
0.001).
Conclusions:
structured
our
shown
have
beneficial
physiological,
improvements
long-COVID,
therefore
it
advisable
these
Язык: Английский