Nanomaterials,
Год журнала:
2023,
Номер
13(21), С. 2893 - 2893
Опубликована: Ноя. 1, 2023
Gap-enhanced
Raman
tags
are
a
new
type
of
optical
probe
that
have
wide
applications
in
sensing
and
detection.
A
gap-enhanced
tag
is
prepared
by
embedding
molecules
inside
gap
between
two
plasmonic
metals
such
as
an
Au
core
shell.
Even
though
placing
beneath
shell
seems
counter-intuitive,
it
has
been
shown
systems
produce
stronger
surface-enhanced
scattering
response
due
to
the
strong
electric
field
gap.
While
theoretical
support
was
provided
literature,
comprehensive
understanding
how
compares
with
outer
surface
particle
not
readily
available.
We
investigated
Au@SiO2@Au
nanoparticles
diameters
ranging
from
35
nm
70
varying
(2.5–10
nm)
(2.5–15
thicknesses
obtained
both
far-field
near-field
spectra.
The
extinction
spectra
these
particles
always
peaks.
low-energy
peak
redshifts
decreasing
thickness.
However,
when
thickness
decreases,
peaks
first
blueshift
then
redshift,
producing
C-shape
position.
For
every
system
we
investigated,
enhancement
were
than
on
nanoparticle.
find
thin
combined
will
greatest
Our
work
fills
knowledge
exciting
potential
fundamental
Chemical Reviews,
Год журнала:
2023,
Номер
123(13), С. 8297 - 8346
Опубликована: Июнь 15, 2023
Omics
technologies
have
rapidly
evolved
with
the
unprecedented
potential
to
shape
precision
medicine.
Novel
omics
approaches
are
imperative
toallow
rapid
and
accurate
data
collection
integration
clinical
information
enable
a
new
era
of
healthcare.
In
this
comprehensive
review,
we
highlight
utility
Raman
spectroscopy
(RS)
as
an
emerging
technology
for
clinically
relevant
applications
using
significant
samples
models.
We
discuss
use
RS
both
label-free
approach
probing
intrinsic
metabolites
biological
materials,
labeled
where
signal
from
reporters
conjugated
nanoparticles
(NPs)
serve
indirect
measure
tracking
protein
biomarkers
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(4), С. 2080 - 2080
Опубликована: Фев. 8, 2024
Immunoassays
(IAs)
with
fluorescence-based
detection
are
already
well-established
commercialized
biosensing
methods,
such
as
enzyme-linked
immunosorbent
assay
(ELISA)
and
lateral
flow
immunoassay
(LFIA).
surface-enhanced
Raman
spectroscopy
(SERS)
have
received
significant
attention
from
the
research
community
for
at
least
two
decades,
but
so
far
they
still
lack
a
wide
clinical
commercial
application.
This
review,
unlike
any
other
review
that
we
seen,
performs
three-dimensional
performance
comparison
of
SERS
IAs
vs.
fluorescence
IAs.
First,
compared
limit
(LOD)
key
parameter
30
SERS-based
immunoassays
reported
in
literature.
We
also
performances
smaller
number
available
reports
(FIAs).
found
median
geometric
average
LODs
about
1.5–2
orders
magnitude
lower
to
immunoassays.
For
instance,
LOD
IA
is
4.3
×
10−13
M,
whereas
FIA,
it
1.5
10−11
M.
However,
there
no
difference
relative
standard
deviation
(RSD)—both
5–6%.
The
analysis
sensitivity,
selectivity,
accuracy
limited
published
studies
FIA
demonstrates
an
advantage
over
terms
value
all
three
those
parameters.
discussed
common
specific
challenges
both
while
proposing
some
solutions
mitigate
techniques.
These
include
non-specific
protein
binding,
interactions
immunoassays,
sometimes
insufficient
reproducibility,
relatively
long
times,
photobleaching,
etc.
Overall,
this
may
be
useful
large
researchers
who
would
like
use
particularly
make
improvements
move
forward
Chemical Science,
Год журнала:
2023,
Номер
14(23), С. 6149 - 6206
Опубликована: Янв. 1, 2023
The
disastrous
spread
of
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
has
induced
public
healthcare
issues
and
weakened
the
global
economy
significantly.
Although
SARS-CoV-2
infection
is
not
as
fatal
initial
outbreak,
many
infected
victims
suffer
from
long
COVID.
Therefore,
rapid
large-scale
testing
critical
in
managing
patients
alleviating
its
transmission.
Herein,
we
review
recent
advances
techniques
to
detect
SARS-CoV-2.
sensing
principles
are
detailed
together
with
their
application
domains
analytical
performances.
In
addition,
advantages
limits
each
method
discussed
analyzed.
Besides
molecular
diagnostics
antigen
antibody
tests,
also
neutralizing
antibodies
emerging
variants.
Further,
characteristics
mutational
locations
different
variants
epidemiological
features
summarized.
Finally,
challenges
possible
strategies
prospected
develop
new
assays
meet
diagnostic
needs.
Thus,
this
comprehensive
systematic
detection
technologies
may
provide
insightful
guidance
direction
for
developing
tools
diagnosis
analysis
support
effective
long-term
pandemic
management
control.
Nano Letters,
Год журнала:
2024,
Номер
24(33), С. 10016 - 10023
Опубликована: Авг. 7, 2024
Food
safety
is
vital
to
human
health,
necessitating
the
development
of
nondestructive,
convenient,
and
highly
sensitive
methods
for
detecting
harmful
substances.
This
study
integrates
cellulose
dissolution,
aligned
regeneration,
in
situ
nanoparticle
synthesis,
structural
reconstitution
create
flexible,
transparent,
customizable,
nanowrinkled
cellulose/Ag
membranes
(NWCM-Ag).
These
three-dimensional
structures
considerably
improve
spatial-electromagnetic-coupling
effect
metal
nanoparticles
on
membrane
surface,
providing
a
2.3
×
10
ACS Applied Nano Materials,
Год журнала:
2024,
Номер
7(2), С. 2335 - 2342
Опубликована: Янв. 5, 2024
In
this
study,
we
introduce
a
simplified
surface-enhanced
Raman
scattering
(SERS)
nanobiosensor
for
precise
detection
of
SARS-CoV-2
antigen,
leveraging
supervised
machine
learning
approaches.
The
biosensor
was
made
with
Au
nanoislands
conjugated
4-aminothiophenol
reporter
and
an
anti-SARS-CoV-2
antibody.
Through
the
integration
feature
selection
algorithms,
namely,
logistic
regression,
linear
discriminant
analysis,
support
vector
machine,
achieved
high
accuracies
ranging
from
96
to
100%
in
antigen
detection.
Furthermore,
identified
underlying
mechanisms
by
employing
concept
multidimensional
calibration
space,
which
is
based
on
decision
trees
random
forest
algorithms.
This
analysis
explainable
allowed
us
gain
insights
into
reasons
why
our
exhibits
lower
sensitivity
compared
that
previous
sandwich-type
immunosensors
SARS-CoV-2.
results
presented
here
emphasize
potential
SERS
biosensing,
can
be
applied
any
type
diagnostics.
Applied Spectroscopy Reviews,
Год журнала:
2023,
Номер
59(2), С. 157 - 186
Опубликована: Янв. 20, 2023
Various
diseases
have
seriously
threatened
the
health
of
Chinese
residents.
Sensitive
and
accurate
analysis
disease
markers
is
very
important
for
early
diagnosis,
treatment,
prognosis
evaluation
diseases.
Therefore,
it
great
clinical
significance
to
explore
a
sensitive,
accurate,
rapid,
noninvasive
detection
technology.
Surface-enhanced
Raman
spectroscopy
(SERS)
uses
light
interact
with
gold,
silver,
other
nanostructured
materials
generate
strong
surface
plasmon
resonance
effect,
which
can
significantly
enhance
signal
molecules
adsorped
on
surface,
obtain
rich
fingerprints
sample
itself
or
probe
super
sensitivity.
This
technology
has
advantages
noninvasiveness,
high
sensitivity,
good
selectivity,
fast
speed,
low
water
interference,
unique
advantages,
so
application
prospect
in
life
science,
testing,
aspects,
become
kind
biological
potential.
paper
mainly
reviewed
research
progress
SERS
serum,
urine,
saliva,
tissue
recent
years
analyzed
problems
challenges
be
solved
by
this
prospected
its
future
development
prospects.
ACS Applied Nano Materials,
Год журнала:
2023,
Номер
6(17), С. 15426 - 15436
Опубликована: Авг. 23, 2023
Surface-enhanced
Raman
scattering
(SERS)-based
immunoassays
have
several
advantages,
such
as
high
sensitivity
and
multiplex
capabilities;
they
are
emerging
a
potential
avenue
for
early
disease
diagnosis
screening.
Here,
we
focused
on
SERS-based
heterogeneous
immunoassays,
in
which
the
number
of
extrinsic
labels
(ERLs)
at
sensor
surface
is
related
to
concentration
intended
target.
The
ERLs
made
gold
nanoparticles
constructed
be
selective
target
boost
signal
reporter.
However,
biomarker
decreases,
per
unit
area
(mm2)
also
leading
small
being
probed
within
an
exciting
laser
spot.
This
poor
sampling
adds
large
intensity
variations
inherent
SERS
effect,
resulting
loss
linearity
between
marker/target
analyte
concentration.
characteristic
has
rendered
unreliable
quantification
low
bioanalyte
concentrations.
We
propose
use
digital
protocol
overcome
this
problem.
A
sandwich
immunoassay
was
developed
detection
SARS-CoV-2
S1–S2
spike
protein
saliva.
conventional
data
analysis
that
relates
intensities
compared
same
dataset.
assay
presented
LOD
6.3
ng·mL–1
or
34.9
pM
LOQ
19.0
105.7
95%
confidence
level.
These
metrics
show
11-fold
improvement
analysis.
