Molecular Understanding of the Surface-Enhanced Raman Spectroscopy Salivary Fingerprint in People after Sars-COV-2 Infection and in Vaccinated Subjects DOI Creative Commons
Francesca Rodà, Alice Gualerzi, Silvia Picciolini

et al.

Chemosensors, Journal Year: 2024, Volume and Issue: 12(7), P. 136 - 136

Published: July 11, 2024

The rapid spread of SARS-COV-2 and the millions worldwide deaths hospitalizations have prompted an urgent need for development screening tests capable rapidly accurately detecting virus, even in asymptomatic people. easy collection biomarker content saliva, together with label-free informative power surface-enhanced Raman spectroscopy (SERS) analysis driven creation point-of-care platforms identifying people COVID-19. Indeed, different salivary fingerprints were observed between uninfected infected Hence, we performed a retrospective SERS spectra from samples COVID-19-infected -vaccinated subjects to understand if viral components and/or immune response are implicated spectral variations. high sensitivity proposed SERS-based method highlighted persistence molecular alterations saliva up one month after first positive swab, when subject tested negative antigenic test. Nevertheless, no specific patterns attributable some proteins immunoglobulins involved COVID-19 infection its progression found, differences peak intensity, presence, position fingerprint.

Language: Английский

The Emerging Role of Raman Spectroscopy as an Omics Approach for Metabolic Profiling and Biomarker Detection toward Precision Medicine DOI
Gabriel Cutshaw, Saji Uthaman, Nora Hassan

et al.

Chemical Reviews, Journal Year: 2023, Volume and Issue: 123(13), P. 8297 - 8346

Published: June 15, 2023

Omics technologies have rapidly evolved with the unprecedented potential to shape precision medicine. Novel omics approaches are imperative toallow rapid and accurate data collection integration clinical information enable a new era of healthcare. In this comprehensive review, we highlight utility Raman spectroscopy (RS) as an emerging technology for clinically relevant applications using significant samples models. We discuss use RS both label-free approach probing intrinsic metabolites biological materials, labeled where signal from reporters conjugated nanoparticles (NPs) serve indirect measure tracking protein biomarkers

Language: Английский

Citations

73
SERS-based microdevices for use as in vitro diagnostic biosensors DOI
Sungwoon Lee,

Hajun Dang,

Joung‐Il Moon

et al.

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: 53(11), P. 5394 - 5427

Published: Jan. 1, 2024

This review explores various microdevices developed for applying SERS technology to in vitro diagnostics and delves into their clinical applications.

Language: Английский

Citations

53
Diagnostics and analysis of SARS-CoV-2: current status, recent advances, challenges and perspectives DOI Creative Commons
Tao Dong, Mingyang Wang,

Junchong Liu

et al.

Chemical Science, Journal Year: 2023, Volume and Issue: 14(23), P. 6149 - 6206

Published: Jan. 1, 2023

The disastrous spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has induced public healthcare issues and weakened the global economy significantly. Although SARS-CoV-2 infection is not as fatal initial outbreak, many infected victims suffer from long COVID. Therefore, rapid large-scale testing critical in managing patients alleviating its transmission. Herein, we review recent advances techniques to detect SARS-CoV-2. sensing principles are detailed together with their application domains analytical performances. In addition, advantages limits each method discussed analyzed. Besides molecular diagnostics antigen antibody tests, also neutralizing antibodies emerging variants. Further, characteristics mutational locations different variants epidemiological features summarized. Finally, challenges possible strategies prospected develop new assays meet diagnostic needs. Thus, this comprehensive systematic detection technologies may provide insightful guidance direction for developing tools diagnosis analysis support effective long-term pandemic management control.

Language: Английский

Citations

38
Recent advances in SERS-based immunochromatographic assay for pathogenic microorganism diagnosis: A review DOI

Qing Yu,

Ting Wu,

Benshun Tian

et al.

Analytica Chimica Acta, Journal Year: 2023, Volume and Issue: 1286, P. 341931 - 341931

Published: Oct. 18, 2023

Language: Английский

Citations

30
Immunoassays: Analytical and Clinical Performance, Challenges, and Perspectives of SERS Detection in Comparison with Fluorescent Spectroscopic Detection DOI Open Access

Xeniya Terzapulo,

Aiym Kassenova,

Rostislav Bukasov

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(4), P. 2080 - 2080

Published: Feb. 8, 2024

Immunoassays (IAs) with fluorescence-based detection are already well-established commercialized biosensing methods, such as enzyme-linked immunosorbent assay (ELISA) and lateral flow immunoassay (LFIA). surface-enhanced Raman spectroscopy (SERS) have received significant attention from the research community for at least two decades, but so far they still lack a wide clinical commercial application. This review, unlike any other review that we seen, performs three-dimensional performance comparison of SERS IAs vs. fluorescence IAs. First, compared limit (LOD) key parameter 30 SERS-based immunoassays reported in literature. We also performances smaller number available reports (FIAs). found median geometric average LODs about 1.5–2 orders magnitude lower to immunoassays. For instance, LOD IA is 4.3 × 10−13 M, whereas FIA, it 1.5 10−11 M. However, there no difference relative standard deviation (RSD)—both 5–6%. The analysis sensitivity, selectivity, accuracy limited published studies FIA demonstrates an advantage over terms value all three those parameters. discussed common specific challenges both while proposing some solutions mitigate techniques. These include non-specific protein binding, interactions immunoassays, sometimes insufficient reproducibility, relatively long times, photobleaching, etc. Overall, this may be useful large researchers who would like use particularly make improvements move forward

Language: Английский

Citations

14
Explainable Machine Learning to Unveil Detection Mechanisms with Au Nanoisland-Based Surface-Enhanced Raman Scattering for SARS-CoV-2 Antigen Detection DOI
Wallance Moreira Pazin, Leonardo N. Furini, Daniel Cesar Braz

et al.

ACS Applied Nano Materials, Journal Year: 2024, Volume and Issue: 7(2), P. 2335 - 2342

Published: Jan. 5, 2024

In this study, we introduce a simplified surface-enhanced Raman scattering (SERS) nanobiosensor for precise detection of SARS-CoV-2 antigen, leveraging supervised machine learning approaches. The biosensor was made with Au nanoislands conjugated 4-aminothiophenol reporter and an anti-SARS-CoV-2 antibody. Through the integration feature selection algorithms, namely, logistic regression, linear discriminant analysis, support vector machine, achieved high accuracies ranging from 96 to 100% in antigen detection. Furthermore, identified underlying mechanisms by employing concept multidimensional calibration space, which is based on decision trees random forest algorithms. This analysis explainable allowed us gain insights into reasons why our exhibits lower sensitivity compared that previous sandwich-type immunosensors SARS-CoV-2. results presented here emphasize potential SERS biosensing, can be applied any type diagnostics.

Language: Английский

Citations

6
The Application of Biomaterials for the Vaccine, Treatment, and Detection of SARS-CoV-2 DOI Creative Commons
Yuan Hu, Lili Liu, Shenghua Zhang

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(5), P. 5175 - 5192

Published: Jan. 26, 2024

The coronavirus disease-19 (COVID-19) pandemic has had a significant impact on human life worldwide since 2019. Specific vaccines and antiviral agents are the most effective means of preventing treating severe acute respiratory syndrome 2 (SARS-CoV-2) infections. Additionally, protective equipment early diagnosis also contribute to controlling spread COVID-19. utilization biomaterials in medicine pharmaceuticals is crucial ensure positive vaccines, agents, equipment. In this review, we discuss application various types biomaterials, including polymers, lipid nanoparticles, inorganic protein- or peptide-associated self-assembled other for vaccine, treatment, prevention Finally, provide perspective future opportunities challenges developing combat viral outbreaks diseases.

Language: Английский

Citations

6
Digital SERS Protocol Using Au Nanoparticle-Based Extrinsic Raman Labels for the Determination of SARS-CoV-2 Spike Protein in Saliva Samples DOI
Ariadne Tückmantel Bido, Alexandre G. Brolo

ACS Applied Nano Materials, Journal Year: 2023, Volume and Issue: 6(17), P. 15426 - 15436

Published: Aug. 23, 2023

Surface-enhanced Raman scattering (SERS)-based immunoassays have several advantages, such as high sensitivity and multiplex capabilities; they are emerging a potential avenue for early disease diagnosis screening. Here, we focused on SERS-based heterogeneous immunoassays, in which the number of extrinsic labels (ERLs) at sensor surface is related to concentration intended target. The ERLs made gold nanoparticles constructed be selective target boost signal reporter. However, biomarker decreases, per unit area (mm2) also leading small being probed within an exciting laser spot. This poor sampling adds large intensity variations inherent SERS effect, resulting loss linearity between marker/target analyte concentration. characteristic has rendered unreliable quantification low bioanalyte concentrations. We propose use digital protocol overcome this problem. A sandwich immunoassay was developed detection SARS-CoV-2 S1–S2 spike protein saliva. conventional data analysis that relates intensities compared same dataset. assay presented LOD 6.3 ng·mL–1 or 34.9 pM LOQ 19.0 105.7 95% confidence level. These metrics show 11-fold improvement analysis.

Language: Английский

Citations

15
Rapid classification of SARS-CoV-2 variant strains using machine learning-based label-free SERS strategy DOI

Jingwang Qin,

Xiangdong Tian, Siying Liu

et al.

Talanta, Journal Year: 2023, Volume and Issue: 267, P. 125080 - 125080

Published: Aug. 17, 2023

Language: Английский

Citations

13
Cellulose Surface Nanoengineering for Visualizing Food Safety DOI

Zewan Lin,

Xiaotong Fu,

Ke Zheng

et al.

Nano Letters, Journal Year: 2024, Volume and Issue: 24(33), P. 10016 - 10023

Published: Aug. 7, 2024

Food safety is vital to human health, necessitating the development of nondestructive, convenient, and highly sensitive methods for detecting harmful substances. This study integrates cellulose dissolution, aligned regeneration, in situ nanoparticle synthesis, structural reconstitution create flexible, transparent, customizable, nanowrinkled cellulose/Ag membranes (NWCM-Ag). These three-dimensional structures considerably improve spatial-electromagnetic-coupling effect metal nanoparticles on membrane surface, providing a 2.3 × 10

Language: Английский

Citations

5