Talanta,
Год журнала:
2024,
Номер
278, С. 126357 - 126357
Опубликована: Июнь 15, 2024
Methamphetamine
(MA)
is
one
of
the
most
virulent
illicit
drugs
that
can
be
synthesized
from
household
materials
leading
to
its
prevalent
trafficking
and
local
manufacturing
in
clandestine
drug
laboratories
(clan
labs).
The
significant
problems
tracing
MA
clan
labs
monitoring
abusers
lie
lag
time
between
sample
collection
analysis
number
tests
done.
Capillary
electrophoresis
(CE)
a
rapid
separation
technique
amenable
miniaturization
field
testing.
Herein,
we
developed
simple
transient
isotachophoretic
(tITP)-CE
method
detect
precursor
pseudoephedrine
(PSE)
non-invasive
biological
fluids.
was
implemented
on
ETD-100,
commercial
fully
automated
portable
CE
instrument
with
an
integrated
swab-based
extraction
system.
Within
2
min
insertion
swab,
PSE
were
automatically
extracted
electrolyte
(LE)
then
separated
covalently
modified
capillaries.
ETD-100
showed
limit
detection
(LOD)
quantification
(LOQ)
0.02
0.05
μg/swab
0.06
PSE,
enhancement
factor
118
328,
respectively,
when
compared
normal
non-tITP
injection.
intra
inter-day
relative
standard
deviation
terms
migration
range
0.75-1.93
%
for
both
2.0-2.4
peak
height.
demonstrated
spiked
different
as
well
fluids
recovery
above
60
%.
Electrophoresis,
Год журнала:
2023,
Номер
45(1-2), С. 165 - 198
Опубликована: Сен. 5, 2023
Abstract
This
review
article
brings
a
comprehensive
survey
of
developments
and
applications
high‐performance
capillary
microchip
electromigration
methods
(zone
electrophoresis
in
free
solution
or
sieving
media,
isotachophoresis,
isoelectric
focusing,
affinity
electrophoresis,
electrokinetic
chromatography,
electrochromatography)
for
analysis,
micropreparation,
physicochemical
characterization
peptides
the
period
from
2021
up
to
ca.
middle
2023.
Progress
study
properties
various
aspects
their
such
as
sample
preparation,
adsorption
suppression,
electroosmotic
flow
regulation,
detection,
are
presented.
New
particular
demonstrated,
several
types
reported.
They
cover
qualitative
quantitative
analysis
synthetic
isolated
determination
complex
biomatrices,
peptide
profiling
biofluids
tissues,
monitoring
chemical
enzymatic
reactions
changes
peptides.
include
also
amino
acid
sequence
peptides,
mapping
proteins,
separation
stereoisomers
chiral
analyses.
In
addition,
micropreparative
separations
interactions
with
other
(bio)molecules
by
above
CE
described.
Toxics,
Год журнала:
2024,
Номер
12(3), С. 185 - 185
Опубликована: Фев. 28, 2024
Chiral
analysis
is
a
very
relevant
topic
in
environmental
chemistry.
This
due
to
the
different
properties
of
stereoisomers
chiral
compounds.
In
case
agrochemicals,
desired
activity,
degradation
rate,
or
toxicity,
among
other
characteristics,
may
differ
between
stereoisomers,
and
same
true
for
emerging
contaminants,
such
as
pharmaceuticals
cosmetics.
Therefore,
development
analytical
methodologies
enabling
their
determination
samples
interest
paramount.
Although
techniques
have
been
widely
employed
carry
out
separations,
HPLC,
GC,
SFC,
capillary
electrophoresis
(CE)
has
attracted
lot
attention
field
its
simplicity,
flexibility,
low
cost.
fact,
chromatographic
columns
are
not
needed,
consumption
reagents
small
dimensions
separation
capillaries.
article
reviews
characteristics
developed
by
CE
stereoselective
pesticides
contaminants
(water
soil),
well
food
commercial
agrochemical
formulations.
Applications
stability
toxicity
studies
these
also
reviewed.
Sensors,
Год журнала:
2024,
Номер
24(2), С. 647 - 647
Опубликована: Янв. 19, 2024
Neurotransmitter
analysis
plays
a
pivotal
role
in
diagnosing
and
managing
neurodegenerative
diseases,
often
characterized
by
disturbances
neurotransmitter
systems.
However,
prevailing
methods
for
quantifying
neurotransmitters
involve
invasive
procedures
or
require
bulky
imaging
equipment,
therefore
restricting
accessibility
posing
potential
risks
to
patients.
The
innovation
of
compact,
vivo
instruments
neurotransmission
holds
the
reshape
disease
management.
This
can
facilitate
non-invasive
uninterrupted
monitoring
levels
their
activity.
Recent
strides
microfabrication
have
led
emergence
diminutive
that
also
find
applicability
vitro
investigations.
By
harnessing
synergistic
microfluidics,
micro-optics,
microelectronics,
this
nascent
realm
research
substantial
promise.
review
offers
an
overarching
view
current
sensing
techniques,
advances
towards
microsensors
tailored
neurotransmission,
state-of-the-art
fabrication
techniques
be
used
fabricate
those
microsensors.
International Journal of Translational Medicine,
Год журнала:
2024,
Номер
4(2), С. 309 - 333
Опубликована: Май 31, 2024
Many
ailments
can
be
diagnosed
while
they
are
asymptomatic,
meaning
that
the
patient
has
no
signs
or
symptoms
of
a
progressing
disease.
If
caught
in
their
initial
stage
formation,
these
disorders
effectively
treated,
leading
to
successful
outcomes;
curative
therapies
halt
illnesses
from
advancing,
thus
improving
quality
life
and
long-term
survival
patient.
Still,
cutting-edge
upgrades
precision
technologies
necessary
for
early,
reliable,
affordable,
rapid
disease
detection,
but
also
vital
well-being
people
future
global
public
health.
The
emerging
role
utility
non-invasive
repeatable
diagnostic
test
approaches
detection
health
conditions
have
been
exemplified
by
liquid
biopsies
based
on
genomic
biomarkers.
As
such,
biological
fluids
permit
any
measurable
molecular
indicator
signature
(e.g.,
proteins)
provide
valuable
information
an
individual’s
wellness
and/or
Among
bodily
secretions
used
diagnostics
is
sputum,
complex
viscous
gel-like
biopolymeric
network
gained
growing
recognition
as
rich
source
biomarkers
airway
infections
pulmonary
diseases,
serves
determinant
reveal
other
illnesses.
per
World
Health
Organization,
burden
respiratory
exacerbated
factors
ranging
considerable
subjection
air
pollution
occupational
contaminants
tobacco
smoking
second-hand
smoke,
addition
poor
socio-economic
status.
Due
likely
increase
determinants,
tract
rise,
affecting
many
individuals,
putting
stress
healthcare
facilities
services
worldwide.
aim
this
study
was
perform
narrative
review
sputum
constituents
with
emphasis
proteins
glycoproteins
assessed
possible
lung
organ
diseases.
A
search
conducted
using
mucus,
proteomics,
biomarkers,
point-of-care
testing
keywords
employing
Google,
PubMed
(MEDLINE),
Web
Science,
selecting
most
referenced
related
papers
last
decade.
We,
therefore,
highlight
need
use
expectorated
induced
specimens
routine
sample
protein
diagnose
chronic
disorders,
predict
inflammation
progression,
well
monitor
effectiveness
treatments.
Further,
we
discuss
urgent
fast
reliable
methods
detect
quantify
crucial
specimens,
limitations
faced
when
dealing
matrices.
Journal of Separation Science,
Год журнала:
2024,
Номер
47(16)
Опубликована: Авг. 1, 2024
This
review
provides
an
overview
of
recent
works
focusing
on
the
determination
amino
acids
(AAs)
and
peptides
using
capillary
electrophoresis
with
contactless
conductivity
detection
ultraviolet
(UV)
detection,
which
is
most
widespread
in
electromigration
techniques,
without
pre-capillary
derivatization.
Available
options
for
UV
these
analytes,
such
as
indirect
complexation
transition
metal
ions,
in-capillary
derivatization
are
described.
Developments
field
direct
UV-absorbing
AAs
well
progress
chiral
separation
A
separate
section
dedicated
to
on-line
sample
preconcentration
methods
combined
electrophoresis-UV.
Advances in Sample Preparation,
Год журнала:
2023,
Номер
7, С. 100082 - 100082
Опубликована: Июль 4, 2023
The
analysis
by
capillary
electrophoresis
(CE)
of
low
abundant
proteins
in
complex
samples,
such
as
biological
fluids
and
food,
is
especially
challenging,
due
to
the
poor
concentration
sensitivity
microscale
separation
techniques
sample
matrix
complexity.
In
order
overcome
these
major
drawbacks,
microextraction
preparation
based
on
on-line
solid-phase
extraction
(SPE-CE)
are
regarded
an
excellent
alternative
for
clean-up
analyte
preconcentration
with
minimum
handling.
this
study,
we
present,
a
tutorial,
valve-free
aptamer
affinity
electrophoresis-mass
spectrometry
(AA-SPE-CE-MS)
method
purification,
preconcentration,
separation,
detection,
characterization
intact
protein
biomarkers
food
using
cases
study
α‑synuclein
(α‑syn),
concanavalin
A
(Con
A),
β-lactoglobulin
(β-LG),
which
related
Parkinson's
disease
allergy,
respectively.
This
tutorial
not
limited
description
analytical
method,
but
it
also
provides
ready-to-use
procedures
sorbent
devices,
introduces
strategies
undesired
effects,
allowing
straightforward
implementation
optimum
performance
AA-SPE-CE-MS,
platform
develop
further
applications.
