Zr‐MOF Carrier‐Enhanced Dual‐Mode Biosensing Platforms for Rapid and Sensitive Diagnosis of Mpox

Advanced Science, Год журнала: 2024, Номер 11(38)

Опубликована: Авг. 9, 2024

Dual-mode readout platforms with colorimetric and electrochemiluminescence (ECL) signal enhancement are proposed for the ultrasensitive flexible detection of monkeypox virus (MPXV) in different scenes. A new nanotag, Ru@U6-Ru/Pt NPs is constructed dual-mode by integrating double-layered ECL luminophores nanozyme using Zr-MOF (UiO-66-NH

Язык: Английский

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Two Birds with one Stone: Dual-mode immunoassay constructed using a novel emitter ethylene glycol-induced perylene diimide and a multifunctional ANS probe

Biosensors and Bioelectronics, Год журнала: 2024, Номер 252, С. 116151 - 116151

Опубликована: Фев. 22, 2024

Язык: Английский

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Au Nanoshell-Based Lateral Flow Immunoassay for Colorimetric and Photothermal Dual-Mode Detection of Interleukin-6

Molecules, Год журнала: 2024, Номер 29(15), С. 3683 - 3683

Опубликована: Авг. 3, 2024

Interleukin-6 (IL-6) detection and monitoring are of great significance for evaluating the progression many diseases their therapeutic efficacy. Lateral flow immunoassay (LFIA) is one most promising point-of-care testing (POCT) methods, yet suffers from low sensitivity poor quantitative ability, which greatly limits its application in IL-6 detection. Hence, this work, we integrated Au

Язык: Английский

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Plasmonic Nanostars: Unique Properties That Distinguish Them from Spherical Nanoparticles from a Biosensing Perspective

Advanced Optical Materials, Год журнала: 2024, Номер 12(28)

Опубликована: Июль 31, 2024

Abstract Over the past three decades, plasmonic nanostructures, particularly spherical ones, have seen remarkable advancements. Recently, attention has shifted toward anisotropic nanoparticles, especially star‐shaped/branched structures such as nanostars (PNS), due to their distinct properties. PNS offers superior electromagnetic enhancement effects, larger surface areas, and well non‐linear unusual photothermal properties, setting them apart from counterparts. Despite significant progress in synthetic methods characterization of particles, challenges remain transitioning technology into practical use. In this perspective article, distinctive attributes biosensing applications are discussed, beginning with an exploration synthesis methodologies. Their optoelectronic properties examined discussed how these influence interaction different molecules a perspective. With focus on PNS, detailed insights offered unique current applications, future potential. By fostering discussion understanding development, article aims facilitate translation encouraging targeted improvements

Язык: Английский

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Structured protein probes modified with selenium nanoparticle for 1-minute measurement of SARS-CoV-2 antigen

Biosensors and Bioelectronics, Год журнала: 2024, Номер 268, С. 116878 - 116878

Опубликована: Ноя. 1, 2024

Conventional point-of-care lateral flow immunoassays are characterized by an antibody-tagged probe irregular coupling that can limit sensitivity and require a long assay's time. We synthesized polyethylene glycol-modified selenium nanoparticles (PEG-SeNPs) template method developed strategy to set antibody probes targeted orderly using PEG-SeNPs. Synthesized PEG-SeNPs with high stability could immobilize antibodies in the "stand-up" orientation, resulting faster detection time of less than 1 min direct observer visualization without instruments or equipment. Results showed SARS-CoV-2 be systematically structured on chip, 10 pg/mL, significantly higher conventional chips. The new device has been validated 192 clinical samples we found 100% negative coincidence, 93.94% positive 95.83% overall coincidence reverse transcriptional PCR test. arrayed probe's allowed detect throat swabs, saliva, serum, fingertip blood samples, mutant strains cross-reactivity common respiratory viruses pathogenic strains, demonstrating promising potential for universal colorimetric platform ultrafast field-deployable diagnostics.

Язык: Английский

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Magnetic Prussian Blue Nanoparticles to Enhance Dual-Readout Lateral Flow Immunoassay for Salmonella Typhimurium Detection

Analytica Chimica Acta, Год журнала: 2025, Номер 1346, С. 343742 - 343742

Опубликована: Янв. 31, 2025

Язык: Английский

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“Three-in-One”: Ultrasensitive Lateral Flow Immunoassay Driven by Magnetic Enrichment and Photothermal Signal Amplification

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(32), С. 18171 - 18180

Опубликована: Авг. 2, 2024

Conventional lateral flow immunoassay (LFIA) usually suffers from poor antimatrix interference, unsatisfactory sensitivity, and lack of quantitative ability for target analyte detection in food matrices. In response to these limits, here, multifunctional nanomaterial ZnFe

Язык: Английский

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Highly sensitive detection of carbendazim in agricultural products using colorimetric and photothermal lateral flow immunoassay based on plasmonic gold nanostars

Talanta, Год журнала: 2024, Номер 281, С. 126891 - 126891

Опубликована: Сен. 14, 2024

Язык: Английский

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Near-infrared responsive gold nanorods for highly sensitive colorimetric and photothermal lateral flow immuno-detection of SARS-CoV-2

Analytical Methods, Год журнала: 2024, Номер 16(16), С. 2597 - 2605

Опубликована: Янв. 1, 2024

We developed a colorimetric and photothermal dual-mode LFIA platform for sensitive selective SARS-CoV-2 N protein detection based on gold nanorods.

Язык: Английский

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Development of Detection Antibody Targeting the Linear Epitope in SARS-CoV-2 Nucleocapsid Protein with Ultra-High Sensitivity

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(8), С. 4436 - 4436

Опубликована: Апрель 18, 2024

The COVID-19 pandemic caused by SARS-CoV-2 highlighted the importance of reliable detection methods for disease control and surveillance. Optimizing antibodies rational screening antigens would improve sensitivity specificity antibody-based such as colloidal gold immunochromatography. In this study, we screened three peptide with conserved sequences in N protein using bioinformatical structural biological analyses. Antibodies that specifically recognize these peptides were prepared. epitope had highest binding affinity its antibody was located on surface protein, which favorable binding. Using optimal can epitope, developed immunochromatography, detect at 10 pg/mL. Importantly, could effectively both natural antigen mutated showing feasibility being applied large-scale population testing SARS-CoV-2. Our study provides a platform reference significance high sensitivity, specificity, reliability other pathogens.

Язык: Английский

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