Molecular characterization of SARS-CoV-2 nucleocapsid protein

Frontiers in Cellular and Infection Microbiology, Год журнала: 2024, Номер 14

Опубликована: Май 23, 2024

Corona Virus Disease 2019 (COVID-19) is a highly prevalent and potent infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Until now, the world still endeavoring to develop new ways diagnose treat COVID-19. At present, clinical prevention treatment of COVID-19 mainly targets spike protein on surface SRAS-CoV-2. However, with continuous emergence SARS-CoV-2 Variants concern (VOC), targeting therapy shows high degree limitation. The Nucleocapsid Protein (N protein) conserved in virus evolution involved key process viral infection assembly. It most expressed structural after humans has immunogenicity. Therefore, N as factor replication basic research application great potential value. This article reviews progress structure biological function protein, diagnosis drug order promote researchers’ further understanding lay theoretical foundation for possible outbreak sudden diseases future.

Язык: Английский

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Current trends in COVID-19 diagnosis and its new variants in physiological fluids: Surface antigens, antibodies, nucleic acids, and RNA sequencing

TrAC Trends in Analytical Chemistry, Год журнала: 2022, Номер 157, С. 116750 - 116750

Опубликована: Авг. 30, 2022

Язык: Английский

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Sub‐lineages of the SARS‐CoV‐2 Omicron variants: Characteristics and prevention

MedComm, Год журнала: 2022, Номер 3(3)

Опубликована: Авг. 16, 2022

Abstract Since the start of coronavirus disease 2019 (COVID‐19) pandemic, new variants severe acute respiratory syndrome 2 (SARS‑CoV‑2) have emerged, accelerating spread virus. Omicron was defined by World Health Organization in November 2021 as fifth “variant concern” after Alpha, Beta, Gamma, and Delta. In recent months, has become main epidemic strain. Studies shown that carries more mutations than Delta, wild‐type, facilitating immune escape its transmission. This review focuses on variant's origin, transmission, biological features, subvariants, mutations, escape, vaccination, detection methods. We also discuss appropriate preventive therapeutic measures should be taken to address challenges posed variant. is valuable guide surveillance, prevention, development vaccines other therapies for variants. It desirable develop a efficient vaccine against variant take effective constrain promote public health.

Язык: Английский

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In Silico Screening of Bioactive Compounds of Representative Seaweeds to Inhibit SARS-CoV-2 ACE2-Bound Omicron B.1.1.529 Spike Protein Trimer

Marine Drugs, Год журнала: 2022, Номер 20(2), С. 148 - 148

Опубликована: Фев. 17, 2022

Omicron is an emerging SARS-CoV-2 variant, evolved from the Indian delta variant B.1.617.2, which currently infecting worldwide. The spike glycoprotein, important molecule in pathogenesis and transmissions of variants, especially omicron B.1.1.529, shows 37 mutations distributed over trimeric protein domains. Notably, fifteen these reside receptor-binding domain may alter transmissibility infectivity. Additionally, evades neutralization more efficiently than spike. Most therapeutic antibodies are ineffective against double immunization with BioNTech-Pfizer (BNT162b2) might not adequately protect severe disease induced by B.1.1.529. So far, no efficient antiviral drugs available omicron. present study identified promising inhibitors seaweed's bioactive compounds to inhibit We have also compared standard ceftriaxone cefuroxime, were suggested as beneficial COVID-19 treatment. Our molecular docking analysis revealed that caffeic acid hexoside (-6.4 kcal/mol; RMSD = 2.382 Å) phloretin (-6.3 0.061 Sargassum wightii (S. wightii) showed inhibitory effect crucial residues ASN417, SER496, TYR501, HIS505, supported for inviolable angiotensin-converting enzyme II (ACE2) receptor interaction. Cholestan-3-ol, 2-methylene-, (3beta, 5 alpha) (CMBA) (-6.0 3.074 Corallina officinalis (C. officinalis) manifested strong RBD mutated LEU452 ALA484, was magnificently observed essential B.1.617.2 previously. (ceftriaxone cefuroxime) or less emphasized pharmacological properties considered chemical compounds. results could be used develop potent seaweed-based and/or dietary supplements treat B.1.1529-infected patients.

Язык: Английский

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From Alpha to Omicron BA.2: New digital RT-PCR approach and challenges for SARS-CoV-2 VOC monitoring and normalization of variant dynamics in wastewater

The Science of The Total Environment, Год журнала: 2022, Номер 848, С. 157740 - 157740

Опубликована: Июль 31, 2022

Язык: Английский

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