Application of Next-Generation Sequencing (NGS) Techniques for Selected Companion Animals
Animals,
Год журнала:
2024,
Номер
14(11), С. 1578 - 1578
Опубликована: Май 27, 2024
Next-Generation
Sequencing
(NGS)
techniques
have
revolutionized
veterinary
medicine
for
cats
and
dogs,
offering
insights
across
various
domains.
In
parasitology,
NGS
enables
comprehensive
profiling
of
parasite
populations,
aiding
in
understanding
transmission
dynamics
drug
resistance
mechanisms.
infectious
diseases,
facilitates
rapid
pathogen
identification,
characterization
virulence
factors,
tracking
outbreaks.
Moreover,
sheds
light
on
metabolic
processes
by
elucidating
gene
expression
patterns
pathways,
essential
diagnosing
disorders
designing
tailored
treatments.
autoimmune
helps
identify
genetic
predispositions
molecular
mechanisms
underlying
immune
dysregulation.
Veterinary
oncology
benefits
from
through
personalized
tumor
profiling,
mutation
analysis,
identification
therapeutic
targets,
fostering
precision
approaches.
Additionally,
plays
a
pivotal
role
genetics,
unraveling
the
basis
inherited
diseases
facilitating
breeding
programs
healthier
animals.
Physiological
investigations
leverage
to
explore
complex
biological
systems,
gene–environment
interactions
pathways
governing
health
disease.
Application
treatment
planning
enhances
efficacy
enabling
strategies
individual
animals
their
ultimately
advancing
care
companion
Язык: Английский
Update on tick-borne pathogens detection methods within ticks
Current Research in Parasitology and Vector-Borne Diseases,
Год журнала:
2024,
Номер
6, С. 100199 - 100199
Опубликована: Янв. 1, 2024
Ticks
are
known
vectors
of
various
pathogens,
including
bacteria,
parasites
and
viruses,
that
impact
both
animal
human
health.
Improving
knowledge
the
distribution
tick-borne
combined
with
their
early
detection
in
ticks,
essential
steps
to
fight
against
diseases
mitigate
impacts.
Here
we
give
an
overview
what
common
methods
pathogen
tick
samples,
recent
developments
concerning
how
handle
get
access
pathogens
by
chemical
or
physical
disruption
used
for
RNA/DNA
extraction
steps.
Furthermore,
discuss
promising
tools
developed
other
sample
types
such
as
serum
blood
detect
those
could
be
future
samples.
Язык: Английский
High Diversity and Prevalence of Rickettsial Agents in Rhipicephalus microplus Ticks from Livestock in Karst Landscapes of Southwest China
Microorganisms,
Год журнала:
2025,
Номер
13(4), С. 765 - 765
Опубликована: Март 27, 2025
Ticks
and
tick-borne
pathogens
pose
a
significant
threat
to
human
animal
health,
yet
the
diversity
prevalence
of
microorganisms
in
karst
regions
remains
inadequately
explored.
In
October
2023,
total
274
Rhipicephalus
microplus
ticks
were
collected
from
livestock
Guizhou
Province,
which
boasts
largest
area
China.
Pathogen
identification
was
subsequently
performed
using
PCR
amplification,
Sanger
sequencing,
phylogenetic
analysis.
High
microbial
noted,
with
five
bacterial
species
order
Rickettsiales
detected,
including
those
genera
Rickettsia
(family
Rickettsiaceae),
Anaplasma,
Ehrlichia
Anaplasmataceae).
The
overall
infection
at
least
one
pathogen
remarkably
high
94.5%.
highest
positive
rate
observed
for
Candidatus
jingxinensis
90.9%.
A
novel
species,
provisionally
designated
as
carsus,
identified
16.8%.
addition,
Anaplasma
marginale,
Ehrlchia
minasensis
canis
detected
15.3%,
4.7%
1.5%,
respectively.
co-infections
involving
two
or
three
rickettsial
34.3%
ticks.
These
findings
highlight
agents
area,
underscoring
need
enhanced
surveillance
effective
tick
control
mitigate
disease
risks
both
humans
livestock.
Язык: Английский
Distribution of Rhipicephalus microplus and Hyalomma lusitanicum, and the pathogens they are carrying: A systematic review
Parasite Epidemiology and Control,
Год журнала:
2025,
Номер
unknown, С. e00437 - e00437
Опубликована: Июнь 1, 2025
Язык: Английский
Investigation of Babesia spp. and Theileria spp. in ticks from Western China and identification of a novel genotype of Babesia caballi
BMC Veterinary Research,
Год журнала:
2024,
Номер
20(1)
Опубликована: Июль 8, 2024
Abstract
Babesia
spp.
and
Theileria
are
tick-borne
protozoan
parasites
with
veterinary
importance.
In
China,
epidemiological
genetic
investigations
on
many
species
were
still
absent
in
areas
tick
species.
From
Aug
2021
to
May
2023,
645
ticks
collected
from
the
body
surface
of
domestic
animals
(camels,
goats,
sheep,
cattle)
using
tweezers
seven
counties
three
provinces
including
Xinjiang
(Qitai,
Mulei,
Hutubi,
Shihezi
counties),
Chongqing
(Youyang
Yunyang
Qinghai
(Huangzhong
county).
Three
morphologically
molecularly
identified
(334
Hyalomma
asiaticum
Xinjiang,
245
Rhipicephalus
microplus
Chongqing,
66
Haemaphysalis
qinghaiensis
Qinghai).
A
total
two
detected
targeting
18S
gene.
The
COI
cytb
sequences
also
recovered
strains
for
further
identification.
R.
bigemina
,
agent
bovine
babesiosis,
was
detected.
Notably,
H.
a
putative
novel
genotype
caballi
(0.90%,
3/334),
whose
genes
have
as
low
85.82%
90.64–90.91%
nucleotide
identities
currently
available
sequences.
It
is
noteworthy
whether
sequence
differences
its
contribute
drug
resistance
this
variant
due
involvement
.
addition,
orientalis
annulata
(12.20%,
31/245)
(1.50%,
5/334),
respectively.
These
results
suggest
that
these
may
be
circulating
areas.
pathogenicity
B.
warrants
investigation.
Язык: Английский