Isolation, Identification, and Antimicrobial Susceptibilities of Bacteria from the Conjunctival Sacs of Dogs with Bacterial Conjunctivitis in Different Regions of Wuhan, China
Veterinary Sciences,
Год журнала:
2025,
Номер
12(1), С. 21 - 21
Опубликована: Янв. 6, 2025
In
order
to
investigate
the
bacterial
species
present
in
conjunctival
sacs
of
dogs
with
conjunctivitis
Wuhan
(Hongshan
District,
Wuchang
Jiangxia
and
Huangpi
District)
their
resistance
aminoglycoside
antibiotics,
samples
sac
secretions
were
collected
from
56
various
regions
Wuhan.
Drug
susceptibility
testing
for
antibiotics
was
performed
on
most
commonly
isolated
gram-positive
gram-negative
bacteria.
The
expression
two
modifying
enzyme
genes,
aacA-aphD
aac
(6')-Ib,
three
16S
rRNA
methyltransferase
rmtB,
rmtE
npmA,
analyzed
by
PCR.
results
showed
that
a
total
123
strains
cultured
secretion
samples,
Staphylococcus
being
species,
followed
Escherichia.
Among
them,
14
pseudointermedius
not
resistant
tobramycin,
amikacin,
gentamicin
or
neomycin,
but
rates
streptomycin
kanamycin
35.71%
42.86%,
respectively.
Escherichia
coli
tobramycin
gentamicin,
they
high
neomycin
(both
at
50%).
detection
rate
gene
100%.
rmtB
85.71%
28.57%,
respectively,
while
aac(6')-Ib
npmA
detected.
Язык: Английский
Pioneering Advances in Veterinary Medicine: From Diagnosis to Treatment
Animals,
Год журнала:
2025,
Номер
15(4), С. 516 - 516
Опубликована: Фев. 12, 2025
Continuous
advancements
in
veterinary
medicine
have
significantly
improved
the
health
and
longevity
of
companion
animals
while
also
contributing
to
wildlife
conservation
efforts
[...]
Язык: Английский
Correction: Domrazek, K.; Jurka, P. Application of Next-Generation Sequencing (NGS) Techniques for Selected Companion Animals. Animals 2024, 14, 1578
Animals,
Год журнала:
2025,
Номер
15(4), С. 606 - 606
Опубликована: Фев. 19, 2025
The
authors
would
like
to
make
the
following
corrections
in
original
publication
[...].
Язык: Английский
Detection of Mycobacteria in Arabian camels and antimycobacterial potential of Moringa oleifera
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Апрель 4, 2025
Язык: Английский
A Framework for Identifying and Prioritizing SNPs in Genes of the Hypothalamic- Pituitary-Gonadal Axis in Guzerat Cattle Using Amplicon-Based NGS
Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Май 19, 2025
Abstract
The
combined
use
of
NGS
technologies
with
bioinformatics
tools
has
significantly
advanced
research
by
enabling
comprehensive
analyses
entire
genomes,
specific
genomic
regions
interest,
and
transcriptomes.
Targeted
methods,
which
focus
on
smaller
genome
fractions,
are
widely
used
to
study
genetic
diseases,
epigenetic
modifications,
microbiomes,
environmental
DNA,
among
other
applications.
This
aimed
develop
a
roadmap
for
detecting
selecting
polymorphisms
in
candidate
genes
integrating
amplicon
NGS-Target
techniques
analyses.
Sixty-eight
associated
the
hypothalamic-pituitary-gonadal
(HPG)
axis
were
selected
assay,
comprising
730
that
cover
total
136,274
bp.
method
was
sequence
75
Guzerat
cattle,
dual-purpose
breed
from
Brazil,
renowned
their
high
rusticity
adaptability.
Zebu
cattle
exhibit
certain
limitations,
such
as
delayed
puberty
onset,
can
reduce
reproductive
efficiency.
Using
GATK
protocol
2,600
SNPs
1,615
indels
detected.
A
series
consecutive
filtering
steps
(maf,
detection
non-synonymous
substitution,
phylogenetic
amino
acid
conservation,
biochemical
properties)
used,
resulting
subset
30
SNPs.
Then,
these
analysed
using
bioinformatic
(SIFT,
PANTHER,
PolyPhen2,
MutPred),
identifying
5
effect
protein.
Their
structure
stability
estimated
AlphaFold
DDMut.
Finally,
3
(
IGF1R,
LHCGR,
TAC3R)
potentially
significant
effects
protein
remained
be
validated
through
dynamic
simulations
or
in
vitro
vivo
experimental
assays.
Язык: Английский
Applications of the powerful next-generation sequencing tool for detecting parasitic infections: towards a smart laboratory platform
Microbial Pathogenesis,
Год журнала:
2025,
Номер
unknown, С. 107746 - 107746
Опубликована: Май 1, 2025
Язык: Английский
Evolution of dirofilariasis diagnostic techniques from traditional morphological analysis to molecular-based techniques: a comprehensive review
Frontiers in Parasitology,
Год журнала:
2024,
Номер
3
Опубликована: Авг. 27, 2024
Dirofilariasis,
caused
by
the
nematode
Dirofilaria
spp.,
poses
significant
challenges
in
diagnosis
due
to
its
diverse
clinical
manifestations
and
complex
life
cycle.
This
comprehensive
literature
review
focuses
on
evolution
of
diagnostic
methodologies,
spanning
from
traditional
morphological
analyses
modern
emerging
techniques
context
dirofilariasis
diagnosis.
The
traces
historical
progression
modalities,
encompassing
approaches
such
as
microscopic
examination,
serological
tests
(including
ELISA
IFA),
radiographic
imaging,
ultrasonography,
necropsy,
which
laid
foundation
for
subsequent
advancements.
integration
molecular
diagnostics
marks
a
turning
point
with
adoption
polymerase
chain
reaction
(PCR)
assays
real-time
PCR
(qPCR)
facilitating
enhanced
sensitivity
specificity.
Furthermore,
recent
strides
next-generation
sequencing
(NGS)
technologies,
including
whole–genome
(WGS),
targeted
(TS),
metagenomic
(MS),
RNA
(transcriptome
sequencing),
have
revolutionized
landscape
diagnostics.
Emerging
loop-mediated
isothermal
amplification
(LAMP),
digital
(dPCR),
microfluidics
are
also
explored
their
potential
augment
accuracy.
addresses
associated
standardizing
protocols,
tackling
false
positives/negatives,
discusses
advantages
limitations
each
technique.
By
providing
overview
strategies,
cutting-edge
methods,
this
aims
enhance
understanding
disease’s
landscape.
insights
gained
implications
improved
disease
management
guide
future
research
endeavors
toward
refining
protocols
advancing
therapeutic
interventions.
Язык: Английский