Macromolecular Bioscience,
Год журнала:
2023,
Номер
23(6)
Опубликована: Март 28, 2023
Abstract
The
cartilage
acellular
matrix
(CAM)
derived
from
porcine
cartilage,
which
does
not
induce
significant
inflammation
and
provides
an
environment
conducive
for
cell
growth
differentiation,
is
a
promising
biomaterial
candidate
scaffold
fabrication.
However,
the
CAM
has
short
period
in
vivo,
vivo
maintenance
controlled.
Therefore,
this
study
aimed
at
developing
injectable
hydrogel
using
CAM.
cross‐linked
with
biocompatible
polyethylene
glycol
(PEG)
cross‐linker
to
replace
typically
used
glutaraldehyde
(GA)
cross‐linker.
cross‐linking
degree
of
by
PEG
(Cx‐CAM‐PEG)
according
ratios
confirmed
contact
angle
heat
capacities
measured
differential
scanning
calorimetry.
Cx‐CAM‐PEG
suspension
exhibits
controllable
rheological
properties
injectability.
Additionally,
suspensions
no
free
aldehyde
group
are
formed
almost
simultaneously
injection.
In
realized
ratio.
certain
host–cell
infiltration
negligible
within
near
transplanted
scaffold.
These
results
suggest
that
suspensions,
safe
represent
potential
candidates
(pre‐)clinical
scaffolds.
Frontiers in Pharmacology,
Год журнала:
2023,
Номер
14
Опубликована: Апрель 21, 2023
Normal
wound
healing
occurs
through
a
careful
orchestration
of
cytokine
and
chemokine
signaling
in
response
to
injury.
Chemokines
are
small
family
chemotactic
cytokines
that
secreted
by
immune
cells
injury
primarily
responsible
for
recruiting
appropriate
cell
types
injured
tissue
at
the
time.
Dysregulation
is
suspected
contribute
delayed
chronic
wounds
diseased
states.
Various
biomaterials
being
used
development
new
therapeutics
our
understanding
their
effects
on
limited.
It
has
been
shown
modifications
physiochemical
properties
can
affect
body’s
reaction.
Studying
these
expression
various
tissues
type
help
us
develop
novel
biomaterial
therapies.
In
this
review,
we
summarize
current
research
available
both
natural
synthetic
healing.
investigation,
conclude
knowledge
chemokines
still
limited
many
fact
share
pro-inflammatory
anti-inflammatory
properties.
The
predominance
either
or
profile
mostly
likely
dependent
timing
after
exposure
biomaterial.
More
needed
better
understand
interaction
contribution
activity
immunomodulatory
effects.
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 14, 2025
Abstract
Studying
the
molecular
basis
of
intestinal
infections
caused
by
enteric
pathogens
at
tissue
level
is
challenging,
because
most
human
infection
models
have
limitations,
and
results
obtained
from
animals
may
not
reflect
situation.
Infections
with
Salmonella
enterica
serovar
Typhimurium
(STm)
different
outcomes
between
organisms.
3D
modeling
primary
material
provides
alternatives
to
animal
experimentation,
but
epithelial
co‐culture
immune
cells
remains
difficult.
Macrophages,
for
instance,
contribute
immunocompetence
native
tissue,
yet
their
incorporation
into
challenging.
A
immunocompetent
model
small
intestine
based
on
decellularized
submucosa
enriched
monocyte‐derived
macrophages
(MDM)
established.
The
multicellular
recapitulated
in
vivo‐like
cellular
diversity,
especially
induction
GP2
positive
microfold
(M)
cells.
Infection
studies
STm
reveal
that
pathogen
physically
interacts
these
M‐like
MDMs
show
trans‐epithelial
migration
phagocytosed
within
levels
inflammatory
cytokines
are
induced
upon
infection.
Infected
shed
supernatant,
potentially
reflecting
an
intracellular
reservoir
invasion‐primed
STm.
Together,
epithelium
bears
potential
as
alternative
identify
human‐specific
processes
underlying
bacterial
infections.
