Optimizing Peptide Crosslinks for Cell-Responsive Hydrogels DOI Creative Commons
Yingjie Wu, Samuel J. Rozans,

Abolfazl Salehi Moghaddam

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 18, 2024

Abstract Cells dynamically modify their local extracellular matrix by expressing proteases that degrade proteins. This enables cells to spread and migrate within tissues, this process is often mimicked in hydrogels through the incorporation of peptide crosslinks can be degraded cell-secreted proteases. However, cleavage hydrogel will also reduce mechanical properties, most crosslinking peptides, such as widely used GPQGIWGQ “PanMMP” sequence, lead bulk degradation hydrogel. A subset are localized cell membrane only active pericellular region immediate vicinity surface. These membrane-type have important physiological roles enable tissues. In work we developed an approach identify optimize sequences specifically We utilized a proteomic screen targets, coupled with functional assay both quantifies individual types elucidate whether peptides primarily cleaved soluble or then split-and-pool synthesis generate more than 300 variants target improve behavior. identified optimized KLVADLMASAE, which proteases, but endothelial stem grown KLVADLMASAE-crosslinked viabilities similar gels crosslinked PanMMP peptide. Notably, biological performance KLVADLMASAE peptide-cross linked was significantly improved from initial found screen. introduces identifying refining protease-substrate way enhance properties matrices.

Язык: Английский

Polymer-Based Hydrogels Applied in Drug Delivery: An Overview DOI Creative Commons
Hoc Thang Nguyen,

Truong Bach Chien,

Đặng Xuân Cường

и другие.

Gels, Год журнала: 2023, Номер 9(7), С. 523 - 523

Опубликована: Июнь 27, 2023

Polymer-based hydrogels are hydrophilic polymer networks with crosslinks widely applied for drug delivery applications because of their ability to hold large amounts water and biological fluids control release based on unique physicochemical properties biocompatibility. Current trends in the development hydrogel systems involve drugs response specific triggers such as pH, temperature, or enzymes targeted reduce potential systemic toxicity. In addition, developing injectable formulations that easily used sustain during this extended time is a growing interest. Another emerging trend synthesis nano other functional substances improving loading efficacy. Following these trends, advanced possessing mechanically improved properties, controlled rates, biocompatibility focus field. More complex multi-drug combination therapies will be developed advancements. polymer-based gaining increasing attention personalized medicine tailored patient, example, combinations, target-specific delivery, improvement disease treatment effectiveness, healthcare cost reduction. Overall, application advancing rapidly, towards more efficient effective future.

Язык: Английский

Процитировано

316
Emerging biomedical technologies for scarless wound healing DOI Creative Commons
Xinyue Cao,

Xiangyi Wu,

Yuanyuan Zhang

и другие.

Bioactive Materials, Год журнала: 2024, Номер 42, С. 449 - 477

Опубликована: Сен. 11, 2024

Язык: Английский

Процитировано

10
Synthetic and natural polymer hydrogels: A review of 3D spheroids and drug delivery DOI
Paramjeet Yadav, Shiwani Singh, Sheetal Jaiswal

и другие.

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 280, С. 136126 - 136126

Опубликована: Сен. 28, 2024

Язык: Английский

Процитировано

9
Engineering models of head and neck and oral cancers on-a-chip DOI Creative Commons
Maurício Gonçalves da Costa Sousa, Sofia M. Vignolo, Cristiane Miranda França

и другие.

Biomicrofluidics, Год журнала: 2024, Номер 18(2)

Опубликована: Март 1, 2024

Head and neck cancers (HNCs) rank as the sixth most common cancer globally result in over 450 000 deaths annually. Despite considerable advancements diagnostics treatment, 5-year survival rate for types of HNCs remains below 50%. Poor prognoses are often attributed to tumor heterogeneity, drug resistance, immunosuppression. These characteristics difficult replicate using vitro or vivo models, culminating few effective approaches early detection therapeutic development. Organs-on-a-chip offer a promising avenue studying HNCs, serving microphysiological models that closely recapitulate complexities biological tissues within highly controllable microfluidic platforms. Such systems have gained interest advanced experimental tools investigate human pathophysiology assess efficacy, providing deeper understanding pathophysiology. This review outlines current challenges opportunities replicating systems, focusing on mimicking soft, glandular, hard head neck. We further delve into major applications organ-on-a-chip including fundamental research, discovery, translational approaches, personalized medicine. emphasizes integration organs-on-a-chip repertoire model available researchers. enables exploration unique aspects thereby accelerating discoveries with potential improve outcomes HNC patients.

Язык: Английский

Процитировано

6
Bioengineered Salivary Gland Microtissues─A Review of 3D Cellular Models and their Applications DOI
Sangeeth Pillai, José G. Munguia-López, Simon D. Tran

и другие.

ACS Applied Bio Materials, Год журнала: 2024, Номер 7(5), С. 2620 - 2636

Опубликована: Апрель 9, 2024

Salivary glands (SGs) play a vital role in maintaining oral health through the production and release of saliva. Injury to SGs can lead gland hypofunction decrease saliva secretion manifesting as xerostomia. While symptomatic treatments for xerostomia exist, effective permanent solutions are still lacking, emphasizing need innovative approaches. Significant progress has been made field three-dimensional (3D) SG bioengineering applications regeneration. This achieved major focus on cell culture techniques, including soluble cues biomaterial components 3D niche. Cells derived from both adult embryonic have highlighted key vitro characteristics models. its first decade exploration, spheroids organoids so far served crucial tools study pathophysiology. review, based literature search over past decade, covers importance types realm their isolation, sourcing, conditions that modulate microenvironment. We discuss different biomaterials employed current advances models using them. The success these cellular further evaluated context organ transplantation disease modeling.

Язык: Английский

Процитировано

4
Promoting Polarization and Differentiation of Primary Human Salivary Gland Stem/Progenitor Cells in Protease-Degradable Hydrogels via ROCK Inhibition DOI
Apoorva S. Metkari, Robert Witt, David M. Cognetti

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Март 17, 2025

Toward the goal of in vitro engineering functional salivary gland tissues, we cultured primary human stem/progenitor cells (hS/PCs) hyaluronic acid-based matrices with varying percentages proteolytically degradable crosslinks presence Rho kinase (ROCK) inhibitor. Single encapsulated hydrogel grew into organized multicellular structures by day 15, and over 60% developed nondegradable 50% hydrogels contained a central lumen. Importantly, ROCK inhibition led to establishment that were correctly polarized, as evidenced apical localization Golgi marker GM130, apical/lateral tight junction protein zonula occludens-1 (ZO-1), basal integrin β1 basement membrane proteins laminin α1 collagen IV. Cultures maintained gels exhibited an increased expression acinar markers aquaporin 5 (AQP5, AQP5) sodium–potassium–chloride cotransporter 1 (SLC12A2, NKCC1) at transcript levels, respectively, compared those without inhibition. Upon stimulation isoproterenol, α-amylase secretion lumen was observed. Particle-tracking microrheology employed analyze stiffness using mitochondria passive tracer particles. Our results indicated grown 100% stiffer than gels, inhibitor softer We conclude reducing cellular contractility via while retaining some degree matrix confinement promotes containing pro-acinar correct apicobasal polarization.

Язык: Английский

Процитировано

0
Current basic and preclinical research for treatment of radiation therapy–induced hyposalivation DOI Creative Commons
Olga J. Baker, Harim Tavares dos Santos, Kihoon Nam

и другие.

JADA Foundational Science, Год журнала: 2025, Номер 4, С. 100046 - 100046

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
A Rapid Manual Solid Phase Peptide Synthesis Method for High‐Throughput Peptide Production DOI Creative Commons
Clyde Overby, Brittany L. Abraham,

Emmanuella Adjei‐Sowah

и другие.

Journal of Biomedical Materials Research Part A, Год журнала: 2025, Номер 113(5)

Опубликована: Май 1, 2025

Solid phase peptide synthesis (SPPS) techniques are critical for developing and using peptides in various biomedical applications. However, typical routes used SPPS either resource-intensive (e.g., with automated or commercial services) time-consuming manual benchtop synthesis). Here, a rapid method was developed to produce up 8 fast cycle times simultaneously. Peptides synthesized manually were of equivalent superior quality those produced by in-house microwave-assisted synthesis, higher average crude purity 70% compared 50%. The significantly reduced time, enabling the parallel coupling amino acids simultaneously 15-20 min, as opposed traditional which requires 80-150 min per acid. This approach offers an intermediate throughput between milligram-scale libraries gram-scale single iteration novel designs without need expensive systems. As result, modifications, including incorporation unnatural acids, can be explored, accelerating development wide range

Язык: Английский

Процитировано

0
Bioprinting salivary gland models and their regenerative applications DOI Creative Commons
Supaporn Klangprapan, Glauco R. Souza, João N. Ferreira

и другие.

BDJ Open, Год журнала: 2024, Номер 10(1)

Опубликована: Май 30, 2024

Abstract Objective Salivary gland (SG) hypofunction is a common clinical condition arising from radiotherapy to suppress head and neck cancers. The radiation often destroys the SG secretory acini, glands are left with limited regenerative potential. Due complex architecture of acini ducts, three-dimensional (3D) bioprinting platforms have emerged spatially define these in vitro epithelial units develop mini-organs or organoids for regeneration. body evidence, this comprehensive review highlights advantages challenges Methods microtissue engineering strategies such as magnetic 3D bioassembly cells microfluidic coaxial cell-laden microfibers microtubes been proposed replace damaged acinar units, avoid use xenogeneic matrices (like Matrigel), restore salivary flow. Results Replacing organ challenging due its architecture, which combines ductal network facilitate unidirectional flow saliva. Our research group was first functional innervation respond both cholinergic adrenergic stimulation. More recently, using hydrogel could also supported formation viable units. Both approaches overcome need Matrigel by facilitating assembly adult stem cells, human dental pulp primary into micro-sized constructs able produce their own matrix self-organize micro-modular tissue clusters lumenized areas. Furthermore, extracellular vesicle (EV) therapies organoid-derived secretome were designed validated ex vivo regeneration after damage. Conclusion Magnetic potential create applications via organoid transplantation EV therapies.

Язык: Английский

Процитировано

2
Novel polymer-based hydrogels of recent research in drug delivery for disease treatment related to SARS-CoV-2 virus DOI Creative Commons
Hoc Thang Nguyen, Nguyen Van Phuc,

Tran Thi Tu Nhi

и другие.

eXPRESS Polymer Letters, Год журнала: 2023, Номер 18(2), С. 160 - 192

Опубликована: Дек. 6, 2023

Polymer-based hydrogels are hydrophilic polymer networks with a remarkable capacity to absorb substantial amounts of water and biological fluids, rendering them highly attractive for drug delivery applications.The COVID-19 pandemic has acted as catalyst research innovation in the realm polymer-based delivery, particular emphasis on antiviral therapeutics, vaccines, diagnostics, precision respiratory system.The distinctive attributes hydrogels, such their biocompatibility, customizable release profiles, ease functionalization, establish versatile platforms development advanced systems combat not only but also spectrum other infectious diseases.This study is dedicated scruti-nizing evaluating characteristics employed treatment diseases associated SARS-CoV-2 virus.Furthermore, investigation introduces novel classification system deployed SARS-CoV-2-related diseases.Additionally, paper provides an up-to-date evaluation latest developed utilized dis-eases linked virus, based conducted through recent months 2023.

Язык: Английский

Процитировано

3