Cathepsin S is a novel target for age-related dry eye

Experimental Eye Research, Год журнала: 2021, Номер 214, С. 108895 - 108895

Опубликована: Дек. 12, 2021

Язык: Английский

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Spatial transcriptomics of the lacrimal gland features macrophage activity and epithelium metabolism as key alterations during chronic inflammation

Frontiers in Immunology, Год журнала: 2022, Номер 13

Опубликована: Окт. 17, 2022

The lacrimal gland (LG) is an exocrine that produces the watery part of tear film lubricates ocular surface. Chronic inflammation, such as Sjögren’s syndrome (SS), one leading causes aqueous-deficiency dry eye (ADDE) disease worldwide. In this study we analyzed chronic inflammation in LGs NOD.B10Sn-H2b/J ( NOD.H-2b ) mice, a mouse model SS, utilizing bulk RNAseq and Visium spatial gene expression. With Seurat performed unsupervised clustering cell distribution expression changes all clusters within LG sections. Moreover, for first time, validated specific pathways defined by using technology to determine activation these This analysis suggests altered metabolism hallmarks inflammatory responses from both epithelial immune cells drive inflammation. most significant pathway enriched upregulated DEGs was “TYROBP Causal Network”, has not been described previously SS. We also noted decrease lipid mice. Our data modulation can provide therapeutic strategy treat ADDE.

Язык: Английский

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The Aging Lacrimal Gland of Female C57BL/6J Mice Exhibits Multinucleate Macrophage Infiltration Associated With Lipid Dysregulation

Investigative Ophthalmology & Visual Science, Год журнала: 2024, Номер 65(6), С. 1 - 1

Опубликована: Июнь 3, 2024

Purpose: Loss of function the lacrimal gland (LG), which produces aqueous tear film, is implicated in age-related dry eye. To better understand this deterioration, we evaluated changes lipid metabolism and inflammation LGs from an aging model. Methods: LG sections female C57BL/6J mice different ages (young, 2–3 months; intermediate, 10–14 old, ≥24 months) were stained with Oil Red-O or Toluidine blue to detect lipids. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis western blotting lysates determined differences expression genes proteins related metabolism. A photobleaching protocol quench autofluorescence was used evaluate immunofluorescence associated NPC1, NPC2, CTSL, macrophages (F4/80, CD11b) age using confocal fluorescence microscopy. Results: Old showed increased lipids prominent basal aggregates acinar cells extra-acinar sites. gene Npc1, Npc2, Lipa, Mcoln2, encoding involved metabolism, age. NPC1 also significantly old by blotting. In photobleached sections, microscopy imaging CTSL age-associated enrichment labeled F4/80. Although mononuclear detectable at all ages, novel multinucleate macrophage population containing enriched F4/80 some CD11b Conclusions: Lipid-metabolizing F4/80-positive multinucleated are adjacent sites deposition acini.

Язык: Английский

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The Extracts of Dendrobium Alleviate Dry Eye Disease in Rat Model by Regulating Aquaporin Expression and MAPKs/NF-κB Signalling

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(19), С. 11195 - 11195

Опубликована: Сен. 23, 2022

Dry eye is one of the most common ocular surface diseases caused by tear film instability and damage due to an abnormal quality or quantity tears. Inflammatory factors can initiate relevant transduction signalling pathways trigger inflammatory cascade response, resulting in inflammation. It has been shown that active ingredients Dendrobium, such as polysaccharides, alkaloids phenols, have anti-inflammatory, anti-tumour immunity-boosting effects, Dendrobium officinale extract improve glandular secretion function, increase salivary expression level water channel protein glands patients with dry syndromes. We investigated vitro cytoprotective effect extracts sodium chloride induced hyperosmotic conditions human cornea keratocytes (HKs). Results showed Kimura et Migo (DOW) loddigesii Rolfe (DLW) could upregulate aquaporins (AQP)5 protein, thus exerting a repairing promoting cell migration. Furthermore, oral administration DOW DLW enhanced production rats exerted protective on damage. AQP5 mucin (muc)5ac proteins lacrimal gland reduce response. inhibited activation corresponding mitogen-activated kinases (MAPK) NF-KB pathway, thereby playing role improving symptoms. This study provides new perspective treatment, may be potential therapeutic agents for eye.

Язык: Английский

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Age-related ocular surface failure: A narrative review

Experimental Eye Research, Год журнала: 2022, Номер 219, С. 109035 - 109035

Опубликована: Март 17, 2022

Язык: Английский

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