Cells,
Год журнала:
2022,
Номер
11(18), С. 2845 - 2845
Опубликована: Сен. 12, 2022
Preservation
of
human
spermatozoa
in
vitro
at
normothermia
or
hypothermia
maintaining
their
functions
and
fertility
for
several
days
plays
a
significant
role
reproductive
biology
medicine.
However,
it
is
well
known
that
left
deteriorate
over
time
irreversibly
as
the
consequence
various
stresses
such
change
osmolarity,
energy
deficiency,
oxidative
damage,
leading
to
substantial
limitations
including
need
semen
examinations,
preservation,
assisted
technology.
These
problems
may
be
addressed
with
aid
non-freezing
storage
techniques.
The
main
most
effective
preservation
strategies
are
partial
total
replacement
seminal
plasma
culture
medium,
named
extenders,
temperature-induced
metabolic
restriction.
Semen
extenders
consist
buffers,
osmolytes,
antioxidants,
etc.
protect
against
above-mentioned
adverse
factors.
Extended
has
negative
effect
on
sperm
parameters,
whereas
its
ART
outcomes
remains
inconsistent.
duration,
temperature,
pre-treatment
should
determined
according
aims
preservation.
Advanced
techniques
nanotechnology
omics
have
been
introduced
show
great
potential
lifespan
extension
sperm.
It
certain
more
patients
will
benefit
from
near
future.
This
review
provided
an
overview
current
knowledge
prospects
prolonged
vitro.
Journal of Extracellular Vesicles,
Год журнала:
2025,
Номер
14(3)
Опубликована: Март 1, 2025
ABSTRACT
Human
transmembrane
serine
protease
2
(TMPRSS2)
has
garnered
substantial
interest
due
to
its
clinical
significance
in
various
pathologies,
notably
pivotal
role
viral
entry
into
host
cells.
The
development
of
effective
strategies
target
TMPRSS2
is
a
current
area
intense
research
and
necessitates
consistent
source
active
with
sufficient
stability.
Here,
we
comprehensively
characterised
human
seminal‐fluid
extracellular
vesicles
(SF‐EVs,
also
referred
as
prostasomes),
bearing
native
surface‐exposed,
enzymatically
demonstrated
by
high‐sensitivity
flow
cytometry
fluorometric
activity
assay.
Additionally,
recombinantly
produced
ectodomain
mammalian
cells
adopting
directed
activation
strategy.
We
observed
comparable
catalytic
parameters
inhibition
characteristics
for
both
SF‐EV‐associated
recombinant
when
exposed
inhibitor
Nafamostat
mesylate.
Leveraging
these
findings,
developed
robust
vitro
biochemical
assay
based
on
SF‐EVs
the
screening
TMPRSS2‐targeting
compounds.
Our
results
will
accelerate
discovery
advancement
efficacious
therapeutic
approaches
targeting
propel
further
exploration
biological
TMPRSS2.
Translational Medicine,
Год журнала:
2025,
Номер
12(1), С. 27 - 36
Опубликована: Май 7, 2025
Background
.
Biological
fluids
contain
diverse
extracellular
vesicle
(EV)
populations
differing
in
size,
origin,
composition,
and
function.
This
study
pioneers
asymmetric
depth
filtration
for
isolating
seminal
plasma
(SP)
EVs,
bypassing
ultracentrifugation
(current
gold
standard).
Objective
Validate
the
novel
method
SP
EV
isolation
as
an
alternative
to
ultracentrifugation.
Design
methods.
Twelve
samples
of
were
collected
from
young
men
patients
advanced
reproductive
age
with
mild
teratozoospermia.
EVs
isolated
using
proposed
method.
Western
blot
analysis
membrane
markers
CD9,
CD63,
CD81
confirmed
presence
studied
samples.
Transmission
electron
microscopy
further
validated
EVs.
Morphological
characteristics
different
groups
described.
Quantitative
assessed
Nanoparticle
Tracking
Analysis
(NTA).
Results
The
obtained
particle
size
concentration
distributions
revealed
age-related
changes
EVs:
group,
a
shift
dis
tribution
peak
toward
larger
particles
was
observed
compared
distribution
group.
Conclusion
results
provide
basis
investigation
exploration
their
potential
clinical
applications
assisted
technologies,
such
vitro
fertilization
programs.
Cells,
Год журнала:
2022,
Номер
11(18), С. 2845 - 2845
Опубликована: Сен. 12, 2022
Preservation
of
human
spermatozoa
in
vitro
at
normothermia
or
hypothermia
maintaining
their
functions
and
fertility
for
several
days
plays
a
significant
role
reproductive
biology
medicine.
However,
it
is
well
known
that
left
deteriorate
over
time
irreversibly
as
the
consequence
various
stresses
such
change
osmolarity,
energy
deficiency,
oxidative
damage,
leading
to
substantial
limitations
including
need
semen
examinations,
preservation,
assisted
technology.
These
problems
may
be
addressed
with
aid
non-freezing
storage
techniques.
The
main
most
effective
preservation
strategies
are
partial
total
replacement
seminal
plasma
culture
medium,
named
extenders,
temperature-induced
metabolic
restriction.
Semen
extenders
consist
buffers,
osmolytes,
antioxidants,
etc.
protect
against
above-mentioned
adverse
factors.
Extended
has
negative
effect
on
sperm
parameters,
whereas
its
ART
outcomes
remains
inconsistent.
duration,
temperature,
pre-treatment
should
determined
according
aims
preservation.
Advanced
techniques
nanotechnology
omics
have
been
introduced
show
great
potential
lifespan
extension
sperm.
It
certain
more
patients
will
benefit
from
near
future.
This
review
provided
an
overview
current
knowledge
prospects
prolonged
vitro.
