Potential of Genome Editing to Improve Aquaculture Breeding and Production

Trends in Genetics, Год журнала: 2019, Номер 35(9), С. 672 - 684

Опубликована: Июль 19, 2019

Aquaculture is the fastest growing food production sector and rapidly becoming primary source of seafood for human diets. Selective breeding programs are enabling genetic improvement traits, such as disease resistance, but progress limited by heritability trait generation interval species. New technologies, genome editing using CRISPR/Cas9 have potential to expedite sustainable in aquaculture. Genome can introduce favorable changes genome, fixing alleles at existing loci, creating de novo alleles, or introducing from other strains The high fecundity external fertilization most aquaculture species facilitate research application a scale that not possible farmed terrestrial animals.

Язык: Английский

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Role and relevance of fish cell lines in advanced in vitro research

Molecular Biology Reports, Год журнала: 2022, Номер 49(3), С. 2393 - 2411

Опубликована: Янв. 11, 2022

Cell line derived from fish has been established as a promising tool for studying many key issues of aquaculture covering growth, disease, reproduction, genetics, and biotechnology. In addition, cell lines are very useful in vitro models toxicological, pathological, immunological studies. The easier maintenance flexible temperature regimes hypoxic conditions make them preferable tools over mammalian lines. Great excitement observed establishing characterizing new representing diverse species tissue types. well-characterized authenticated utmost essential these represent cellular functions similar to vivo state an organism otherwise it would affect the reproducibility scientific research. have exhibited encouraging results several aspects research including virology, nutrition metabolism, production vaccines, transgenic production. review paper reports developed fish, their characterization, biobanking along with potential applications challenges

Язык: Английский

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Identification of Fish Species and Targeted Genetic Modifications Based on DNA Analysis: State of the Art

Foods, Год журнала: 2023, Номер 12(1), С. 228 - 228

Опубликована: Янв. 3, 2023

Food adulteration is one of the most serious problems regarding food safety and quality worldwide. Besides misleading consumers, it poses a considerable health risk associated with potential non-labeled allergen content. Fish fish products are expensive widely traded commodities, which predisposes them to being adulterated. Among all fraud types, replacing high-quality or rare less valuable species predominates. Because differ in their content, specifically main one, parvalbumin, replacement can endanger consumers. This underlines need for reliable, robust control systems identification. Various methods may be used aforementioned purpose. DNA-based favored due characteristics target molecule, DNA, heat resistant, fact that through its sequencing, several other traits, including recognition genetic modifications, determined. Thus, they considered powerful tools identifying cases fraud. In this review, major applicable meat product authentication commercial applications discussed, possibilities detecting modifications evaluated, future trends highlighted, emphasizing comprehensive regularly updated online database resources.

Язык: Английский

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Advancing genetic improvement in the omics era: status and priorities for United States aquaculture

BMC Genomics, Год журнала: 2025, Номер 26(1)

Опубликована: Фев. 17, 2025

The innovations of the "Omics Era" have ushered in significant advancements genetic improvement agriculturally important animal species through transforming genetics, genomics and breeding strategies. These were often coordinated, part, by support provided over 30 years 1993-2023 National Research Support Project 8 (NRSP8, Animal Genome Program, NAGRP) affiliate projects focused on enabling genomic discoveries livestock, poultry, aquaculture species. parallel advances demand strategic planning future research priorities. This paper, as an output from May 2023 Aquaculture Genomics, Genetics, Breeding Workshop, provides updated status resources for United States species, highlighting major achievements emerging Finfish shellfish genome omics enhance our understanding architecture heritability performance production traits. Workshop identified present aims genomics/omics to build this progress: (1) advancing reference assembly quality; (2) integrating multi-omics data analysis traits; (3) developing collection integration phenomics data; (4) creating pathways applying information across industries; (5) providing training, extension, outreach application phenome. focuses should emphasize collection, artificial intelligence, identifying causative relationships between genotypes phenotypes, establishing apply tools industries, expansion training programs next-generation workforce facilitate sciences into operations productivity, competitiveness, sustainability. collective vision with focus highlighted priorities is intended continued advancement genomics, genetics community industries. Critical challenges ahead include practical analytical frameworks beyond academic communities that require collaborative partnerships academia, government, industry. scope review encompasses use applications study aquatic animals cultivated human consumption settings throughout their life-cycle.

Язык: Английский

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Application of Gene Editing for Climate Change in Agriculture

Frontiers in Sustainable Food Systems, Год журнала: 2021, Номер 5

Опубликована: Сен. 7, 2021

Climate change imposes a severe threat to agricultural systems, food security, and human nutrition. Meanwhile, efforts in crop livestock gene editing have been undertaken improve performance across range of traits. Many the targeted phenotypes include attributes that could be beneficial for climate adaptation. Here, we present examples emerging applications research initiatives are aimed at improvement crops response change, discuss technical limitations opportunities therein. While only few translated production thus far, numerous studies settings demonstrated potential potent address near future.

Язык: Английский

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Genome editing and its applications in genetic improvement in aquaculture

Reviews in Aquaculture, Год журнала: 2021, Номер 14(1), С. 178 - 191

Опубликована: Июль 13, 2021

Abstract In the aquaculture industry, selective breeding has played an important role in increasing production significantly. To meet global growing demand for high quality proteins, it is essential to apply novel technologies accelerate facilitate increase production. Gene and genome editing technologies, including ZFNs, TALLENS Crispr/Cas9, are promising tools speed up genetic improvement. Among all approaches, CRISPR/Cas9 system faster, cheaper precise genes/genomes. Therefore, application of technology genomes species emerging rapidly. It been applied precisely edit genes identify gene functions generate preferred traits over 20 species. This review summarises their applications rapid improvement economic industry. Challenges future directions also discussed.

Язык: Английский

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Genome editing on finfish: Current status and implications for sustainability

Reviews in Aquaculture, Год журнала: 2021, Номер 13(4), С. 2344 - 2363

Опубликована: Май 26, 2021

Abstract Novel genome editing techniques allow for efficient and targeted improvement of aquaculture stock might be a solution to solve challenges related disease environmental impacts. This review has retrieved the latest research on aquacultured finfish species, exploring technological progress scope. Genome most often been used Nile tilapia ( Oreochromis niloticus Linnaeus), followed by Atlantic salmon (Salmo salar Linnaeus). More than half studies have focused developing solutions challenges, while rest can characterized as basic fish genetics/physiology or technology development. Main traits researched are reproduction development, growth, pigmentation, resistance, use trans‐GFP study omega‐3 metabolism, respectively. There is certain correlation between species identified their commercial relevance, indicating relevance present aquaculture. Reviewing geographical origin research, China in forefront (29 publications), United States (9) Norway (7). The seems not dependent regulative conditions respective countries, but merely purpose objectives technologies. Some technical barriers presented together with overcome these‐off‐target effects, ancestral duplication mosaicism F0. One contribution more sustainable aquaculture, where prominent issues that contribute minimizing impact biodiversity.

Язык: Английский

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Sustainable use of CRISPR/Cas in fish aquaculture: the biosafety perspective

Transgenic Research, Год журнала: 2021, Номер 31(1), С. 1 - 21

Опубликована: Июль 25, 2021

Abstract Aquaculture is becoming the primary source of seafood for human diets, and farmed fish aquaculture one its fastest growing sectors. The industry currently faces several challenges including infectious parasitic diseases, reduced viability, fertility reduction, slow growth, escapee environmental pollution. commercialization growth-enhanced AquAdvantage salmon CRISPR/Cas9-developed tilapia ( Oreochromis niloticus ) proffers genetic engineering genome editing tools, e.g. CRISPR/Cas, as potential solutions to these challenges. Future traits being developed in different species include disease resistance, sterility, enhanced growth. Despite notable advances, off-target effect non-clarification trait-related genes among other technical hinder full realization CRISPR/Cas potentials breeding. In addition, current regulatory risk assessment frameworks are not fit-for purpose regarding notwithstanding that public acceptance key products new technology. this study, we discuss how can be used overcome some limitations focusing on diseases release aquaculture. We further present limitations, use proffer research strategies will provide much-needed data decisions, assessments, increased awareness sustainable applications with emphasis Atlantic Salmo salar

Язык: Английский

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Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-Associated Protein and Its Utility All at Sea: Status, Challenges, and Prospects

Microorganisms, Год журнала: 2024, Номер 12(1), С. 118 - 118

Опубликована: Янв. 6, 2024

Initially discovered over 35 years ago in the bacterium Escherichia coli as a defense system against invasion of viral (or other exogenous) DNA into genome, CRISPR/Cas has ushered new era functional genetics and served versatile genetic tool all branches life science. revolutionized methodology gene knockout with simplicity rapidity, but it is also powerful for knock-in modification. In field marine biology ecology, this been instrumental characterization ‘dark’ genes documentation differentiation paralogs. Powerful is, challenges exist that have hindered advances some important lineages. This review examines status applications research assesses prospect quickly expanding deployment to address myriad fundamental biological oceanography questions.

Язык: Английский

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Genetic improvement in edible fish: status, constraints, and prospects on CRISPR-based genome engineering

3 Biotech, Год журнала: 2024, Номер 14(2)

Опубликована: Янв. 18, 2024

Язык: Английский

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