Aquaculture Reports,
Год журнала:
2024,
Номер
36, С. 102120 - 102120
Опубликована: Май 8, 2024
Sepia
esculenta
is
a
tasty
and
nutritious
cephalopod.
But
the
trend
toward
global
warming
accelerating
with
massive
emissions
of
greenhouse
gases.
An
increase
in
seawater
temperature
decrease
pH
are
great
challenge
for
artificial
breeding
culture
S.
esculenta.
In
this
study,
we
investigated
contingency
mechanism
larvae
under
both
high
low
conditions
by
transcriptome
analysis.
A
total
1235
DEGs
were
identified.
functional
enrichment
analysis
results
suggest
that
led
to
malignant
proliferation
exceptional
cells
larvae.
comprehensive
KEGG
signaling
pathway
protein-protein
interaction
network
(PPI)
identified
sixteen
key
genes
conditions.
The
three
highest
quantities
interactions
or
involved
more
pathways
as
hub
affecting
normal
physiological
processes
effects
ocean
acidification
on
metabolism
immunity
preliminarily
provide
necessary
help
captive
face
climate
change.
Frontiers in Immunology,
Год журнала:
2024,
Номер
15
Опубликована: Июль 8, 2024
Introduction
High-alkalinity
water
is
a
serious
health
hazard
for
fish
and
can
cause
oxidative
stress
metabolic
dysregulation
in
livers.
However,
the
molecular
mechanism
of
liver
damage
caused
by
high
alkalinity
unclear.
Methods
In
this
study,
180
carp
were
randomly
divided
into
control
(C)
group
high-alkalinity
(A25)
cultured
56
days.
High-alkalinity-induced
injury
was
analysed
using
histopathological,
whole-transcriptome,
metabolomic
analyses.
Results
Many
autophagic
bodies
abundant
mitochondrial
membrane
observed
A25
group.
High
decreased
superoxide
dismutase
(SOD),
catalase
(CAT),
glutathione
peroxidase
(GSH-Px)
activity
total
antioxidant
capacity
(T-AOC)
increased
malondialdehyde
(MDA)
content
tissues,
causing
liver.
Transcriptome
analysis
revealed
61
differentially
expressed
microRNAs
(miRNAs)
4008
mRNAs.
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
enrichment
that
mammalian
target
rapamycin
(mTOR),
forkhead
box
O
(FoxO),
mitogen-activated
protein
kinase
(MAPK),
autophagy
signalling
pathway
mechanisms
involved.
causes
results
Bioinformatic
predictions
indicated
Unc-51
Like
Kinase
2
(ULK2)
potential
gene
miR-140-5p,
demonstrating
triggered
through
miR-140-5p–ULK2
axis.
Metabolomic
concentrations
cortisol
21-sulfate
beta-aminopropionitrile
significantly
increased,
while
those
creatine
uracil
decreased.
Discussion
The
effects
on
whole-transcriptome
miRNA-mRNA
networks
metabolomics
approaches.
Our
study
provides
new
insights
highly
alkaline
water.
Poultry Science,
Год журнала:
2025,
Номер
104(4), С. 104965 - 104965
Опубликована: Март 1, 2025
Ammonia
(NH3)
and
lipopolysaccharide
(LPS),
common
pollutants
in
poultry
farming
environments,
pose
significant
health
risks
by
disrupting
cellular
processes.
Although
previous
studies
have
demonstrated
the
individual
effect
of
NH3
or
LPS
on
human
animal
health,
mechanisms
underlying
their
combined
impact
chicken
heart
tissue
remain
poorly
understood.
In
this
study,
we
established
a
cardiotoxicity
model
to
investigate
effects
and/or
exposure
energy
metabolism,
autophagy,
endoplasmic
reticulum
(ER)
stress,
apoptosis
cardiomyocytes.
Our
findings
indicated
that
or/and
reduced
ATPase
activity
ATP
content,
led
downregulation
HK2,
PK,
PDHX,
SDH,
upregulation
AMPK,
resulting
impaired
metabolism
Additionally,
found
gga-miR-1599/HK2
axis
as
key
regulator
involved
LPS-induced
impairment.
The
impairment
activated
AMPK/mTOR
pathway,
which
subsequently
triggered
evidenced
Beclin,
LC3-I,
LC3-II.
Furthermore,
decreased
mTOR
expression
induced
ER
markers
such
ATF6,
GRP78,
IRE1,
PERK.
turn,
increased
CHOP
expression,
downregulated
Bcl-2
upregulated
Bim,
elevated
levels
Bax,
caspase-9,
caspase-3,
ultimately
triggering
apoptosis.
This
study
provides
valuable
insights
into
co-exposure
identifies
potential
molecular
targets
for
mitigating
these
adverse
effects.
