Exosome/antimicrobial peptide laden hydrogel wound dressings promote scarless wound healing through miR-21-5p-mediated multiple functions

Biomaterials, Год журнала: 2024, Номер 308, С. 122558 - 122558

Опубликована: Апрель 2, 2024

Язык: Английский

---

Thou shall not heal: Overcoming the non-healing behaviour of diabetic foot ulcers by engineering the inflammatory microenvironment

Advanced Drug Delivery Reviews, Год журнала: 2023, Номер 203, С. 115120 - 115120

Опубликована: Окт. 25, 2023

Diabetic foot ulcers (DFUs) are a devastating complication for diabetic patients that have debilitating effects and can ultimately lead to limb amputation. Healthy wounds progress through the phases of healing leading tissue regeneration restoration barrier function skin. In contrast, in dysregulation these leads chronic, non-healing wounds. particular, unresolved inflammation DFU microenvironment has been identified as key facet chronic hyperglyceamic patients, DFUs fail beyond inflammatory phase towards resolution. Thus, control over modulation response is promising therapeutic avenue treatment. This review discusses current state-of-the art regarding microenvironment, with specific focus on development biomaterials-based delivery strategies their cargos direct microenvironment.

Язык: Английский

---

Engineered exosomes as a prospective therapy for diabetic foot ulcers

Burns & Trauma, Год журнала: 2024, Номер 12

Опубликована: Янв. 1, 2024

Abstract Diabetic foot ulcer (DFU), characterized by high recurrence rate, amputations and mortality, poses a significant challenge in diabetes management. The complex pathology involves dysregulated glucose homeostasis leading to systemic local microenvironmental complications, including peripheral neuropathy, micro- macro-angiopathy, recurrent infection, persistent inflammation re-epithelialization. Novel approaches accelerate DFU healing are actively pursued, with focus on utilizing exosomes. Exosomes natural nanovesicles mediating cellular communication containing diverse functional molecular cargos, DNA, mRNA, microRNA (miRNA), lncRNA, proteins, lipids metabolites. While some exosomes show promise modulating function promoting healing, their efficacy is limited low yield, impurities, loading content inadequate targeting. Engineering enhance curative activity represents potentially more efficient approach for DFUs. This could facilitate focused repair regeneration of nerves, blood vessels soft tissue after development. review provides an overview pathogenesis, strategies exosome engineering the targeted therapeutic application engineered addressing critical pathological changes associated

Язык: Английский

---

Microenvironment Remodeling Self-Healing Hydrogel for Promoting Flap Survival

Biomaterials Research, Год журнала: 2024, Номер 28

Опубликована: Янв. 1, 2024

Random flap grafting is a routine procedure used in plastic and reconstructive surgery to repair reconstruct large tissue defects. Flap necrosis primarily caused by ischemia–reperfusion injury inadequate blood supply the distal flap. Ischemia–reperfusion leads production of excessive reactive oxygen species, creating pathological microenvironment that impairs cellular function angiogenesis. In this study, we developed remodeling self-healing hydrogel [laminarin–chitosan-based hydrogel-loaded extracellular vesicles ceria nanozymes (LCH@EVs&CNZs)] improve synergistically promote regeneration survival. The natural (LCH) was created oxidation laminarin carboxymethylated chitosan via Schiff base reaction. We loaded with CNZs EVs. are class nanomaterials enzymatic activity known for their strong scavenging capacity thus alleviating oxidative stress. EVs cell-secreted vesicular structures containing thousands bioactive substances can cell proliferation, migration, differentiation, constructed LCH@EVs&CNZs demonstrated robust excess thereby conferring protection stress environments. Moreover, these constructs notably enhance migration Our results demonstrate effectively remodel skin marked This approach introduces new therapeutic strategy combining microenvironmental EV therapy, which holds promise promoting

Язык: Английский

---

Immunomodulation effects of collagen hydrogel encapsulating extracellular vesicles derived from calcium silicate stimulated-adipose mesenchymal stem cells for diabetic healing

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Янв. 27, 2025

Diabetic wounds are characterized by chronic inflammation, reduced angiogenesis, and insufficient collagen deposition, leading to impaired healing. Extracellular vesicles (EVs) derived from adipose-derived mesenchymal stem cells (ADSC) offer a promising cell-free therapeutic strategy, yet their efficacy immunomodulation can be enhanced through bioactivation. In this study, we developed calcium silicate (CS)-stimulated ADSC-derived EVs (CSEV) incorporated into hydrogels create sustained-release system for promoting diabetic wound CSEV exhibited protein content, surface marker expression, bioactive cargo enriched with pro-angiogenic anti-inflammatory factors. vitro, CSEV-loaded significantly reactive oxygen species production, promoted cell proliferation migration compared standard EV-loaded collagen. Cytokine profiling revealed the upregulation of cytokines extracellular matrix components, highlighting immunomodulatory regenerative potential. vivo, histological evaluation rabbit models treated superior reepithelialization organized indicating accelerated closure. These findings underscore potential as an innovative effective platform enhancing healing simultaneously addressing inflammation tissue regeneration.

Язык: Английский

---

Advancements and Insights in Exosome-Based Therapies for Wound Healing: A Comprehensive Systematic Review (2018–June 2023)

Biomedicines, Год журнала: 2023, Номер 11(8), С. 2099 - 2099

Опубликована: Июль 25, 2023

Exosomes have shown promising potential as a therapeutic approach for wound healing. Nevertheless, the translation from experimental studies to commercially available treatments is still lacking. To assess current state of research in this field, systematic review was performed involving conducted and published over past five years. A PubMed search English-language, full-text papers 2018 June 2023, focusing on exosomes derived mammalian sources their application healing, particularly those vivo assays. Out 531 results, 148 were selected analysis. The findings revealed that exosome-based improve healing by increasing angiogenesis, reepithelization, collagen deposition, decreasing scar formation. Furthermore, there significant variability terms cell types, biomaterials, administration routes under investigation, indicating need further field. Additionally, comparative examination encompassing diverse cellular origins, pathways, or biomaterials imperative. predominance rodent-based animal models raises concerns, been limited advancements towards more complex scale-up These constraints underscore substantial efforts remain necessary before attaining viable extensively applicable approaches using exosomes.

Язык: Английский

---

Natural polymer nanofiber dressings for effective management of chronic diabetic wounds: A comprehensive review

International Journal of Biological Macromolecules, Год журнала: 2024, Номер unknown, С. 136688 - 136688

Опубликована: Окт. 1, 2024

Язык: Английский

---

Exosomes Exposed: Overview Systematic Review on Evidence Versus Expectation in Aesthetic and Regenerative Medicine

Aesthetic Plastic Surgery, Год журнала: 2024, Номер unknown

Опубликована: Июль 29, 2024

Язык: Английский

---

A flexible adhesive hydrogel dressing of embedded structure with pro-angiogenesis activity for wound repair at moving parts inspired by commercial adhesive bandages

Materials Today Advances, Год журнала: 2023, Номер 21, С. 100452 - 100452

Опубликована: Дек. 6, 2023

Compared with the wound in flat part of human body, repair joint, armpit and other frequently moving parts is still a complex problem. Although many flexible adhesive hydrogel dressings for wounds at have been developed, order to improve their flexibility adhesion, most use synthetic polymers natural form composite hydrogels, which greatly reduces biocompatibility bioactivity compared single polymer hydrogel. They can only passively provide barrier process slow, seriously hinders further application. Inspired by commercial bandages, we successfully constructed dressing embedded structure pro-angiogenesis activity. The was prepared non-adhesive topological adhesion molecular entanglement. Due high-density hydrogen bonding, hydrogels possessed good flexibility, allowed them successfully. In addition, loaded exosomes directly contact wound, minimizing stimulation tissue cytotoxic materials, continuously releasing active substances promote vascular regeneration. This biocompatible activity shows strong potential remodeling, providing new strategy treatment or sensitive parts.

Язык: Английский

---

MicroRNA and Protein Cargos of Human Limbal Epithelial Cell-Derived Exosomes and Their Regulatory Roles in Limbal Stromal Cells of Diabetic and Non-Diabetic Corneas

Cells, Год журнала: 2023, Номер 12(21), С. 2524 - 2524

Опубликована: Окт. 25, 2023

Epithelial and stromal/mesenchymal limbal stem cells contribute to corneal homeostasis cell renewal. Extracellular vesicles (EVs), including exosomes (Exos), can be paracrine mediators of intercellular communication. Previously, we described cargos regulatory roles stromal (LSC)-derived Exos in non-diabetic (N) diabetic (DM) epithelial (LECs). Presently, quantify the miRNA proteome profiles human LEC-derived their N- DM-LSC. We revealed some protein differences DM vs. N-LEC-derived Exos' cargos, proteins involved Exo biogenesis packaging that may affect production ultimately cellular crosstalk function. Treatment by N-Exos, but not DM-Exos, enhanced wound healing cultured N-LSCs increased proliferation rates N LSCs corresponding untreated (control) cells. N-Exos-treated reduced keratocyte markers ALDH3A1 lumican MSC CD73, CD90, CD105 control LSCs. These being opposite changes quantified wounded Overall, N-LEC have a more pronounced effect on LSC healing, proliferation, marker expression than DM-LEC Exos. This suggests cargo DM- could disease state.

Язык: Английский

---