MicroRNA Expression in Chronic Venous Leg Ulcers and Implications for Wound Healing: A Scoping Review DOI
Magali Rezende de Carvalho, Hyehwan Yang,

Joyce K. Stechmiller

и другие.

Biological Research For Nursing, Год журнала: 2024, Номер unknown

Опубликована: Окт. 16, 2024

Purpose: Chronic venous leg ulcers (CVLUs) comprise the majority of lower-extremity wounds, yet their pathophysiology is not fully understood. While research has shown that microRNAs are an important component wound inflammation, few have explored role (miRNAs) in healing CVLUs. This scoping review examines miRNAs CVLUs and association with healing. Methods: In December 2023, we searched MEDLINE/PubMed, Embase, Scopus, CINAHL for studies published 2013–2023 examining CVLU Results: Six met inclusion criteria. MicroRNAs were extracted from various specimens including serum, skin biopsy samples, adipose tissue-derived mesenchymal cells individuals Overexpression miR-221, miR-222, miR-92a, miR-301a-3p hindered angiogenesis, while overexpression miR-296, miR-126, miR-378, miR-210 facilitated angiogenesis. miR-34a/c, miR-301a-3p, miR-450-5p, miR-424-5p, miR-516-5p, miR-7704 increased local inflammatory responses inhibited keratinocytes proliferation, impairing healing, miR-19a/b miR-20 downregulated keratinocytes' response, promoting Downregulation miR-205, miR-96-5p, miR-218-5p enhanced cellular proliferation promoted miR-17–92 was linked impaired Discussion: play a regulating responses, cell migration chronic-wound However, limited lack standardized approach to measurement quantification. Further warranted elucidate mechanisms underlying microRNA involvement better understand future development targeted therapies.

Язык: Английский

Food-derived tripeptide–copper self-healing hydrogel for infected wound healing DOI Creative Commons

Han Chen,

Pu Yang,

Ping Xue

и другие.

Biomaterials Research, Год журнала: 2025, Номер 29

Опубликована: Янв. 1, 2025

The field of infected wound management continues to face challenges, and traditional methods used cope with wounds include debridement, gauze coverage, medication, others. Currently, synthetic natural biomaterials are readily available today, enabling the creation new dressings that substantially enhance healing. Considerable attention is being paid hydrogels based on materials, which have good biocompatibility degradability properties, while exhibiting higher similarity extracellular matrix as compared materials. In this study, we extracted active ingredients oxidized konjac glucomannan (OKGM) fresh egg white (EW) from 2 foods, konjac, egg, respectively, formed a self-repairing hydrogel cross-linking Schiff base. Subsequently, peptide, glycyl-l-histidyl-l-lysine-Cu (GHK-Cu), was loaded, an all-natural composite dressing, EW/OKGM@GHK-Cu (GEK), developed. GEK hydrogel, both antibacterial anti-inflammatory plays hemostatic role by adhering tissues promoting neovascularization serves optimal dressing for skin regeneration. Taken together, derived food sources therefore constitute efficient cost-effective strategy managing healing significant potential clinical application transformation.

Язык: Английский

Процитировано

4
Multifunctional hydrogel-based engineered extracellular vesicles delivery for complicated wound healing DOI Creative Commons
Zuhao Li,

Jinlong Liu,

Jian Song

и другие.

Theranostics, Год журнала: 2024, Номер 14(11), С. 4198 - 4217

Опубликована: Янв. 1, 2024

The utilization of extracellular vesicles (EVs) in wound healing has been well-documented.However, the direct administration free EVs via subcutaneous injection at sites may result rapid dissipation bioactive components and diminished therapeutic efficacy.Functionalized hydrogels provide effective protection, as well ensure sustained release bioactivity during process, making them an ideal candidate material for delivering EVs.In this review, we introduce mechanisms by which accelerate healing, then elaborate on construction strategies engineered EVs.Subsequently, discuss synthesis application delivery systems to enhance complicated healing.Furthermore, face wounds, functionalized with specific microenvironment regulation capabilities, such antimicrobial, anti-inflammatory, immune regulation, used loading EVs, potential approaches addressing these challenges.Ultimately, deliberate future trajectories outlooks, offering a fresh viewpoint advancement artificial intelligence (AI)-energized materials 3D bio-printed multifunctional hydrogel-based dressings biomedical applications.

Язык: Английский

Процитировано

13
Small extracellular vesicles-shuttled miR-23a-3p from mesenchymal stem cells alleviate renal fibrosis and inflammation by inhibiting KLF3/STAT3 axis in diabetic kidney disease DOI Creative Commons
Qianhua Li, Jiaxi Liu,

Rongyun Su

и другие.

International Immunopharmacology, Год журнала: 2024, Номер 139, С. 112667 - 112667

Опубликована: Июль 16, 2024

Human umbilical cord mesenchymal stem cells-derived small extracellular vesicles (MSC-sEV) provide a pragmatic solution as cell-free therapy for patients with diabetic kidney disease (DKD). However, the underlying protective mechanisms of MSC-sEV remain largely unknown in DKD. Invivo and vitro analyses demonstrated that attenuated renal fibrosis inflammation The mechanism MSC-sEV-induced therapeutic effect was explored by high-throughput sequencing, which identified unique enrichment set miRNAs compared human skin fibroblasts-sEV (HSF-sEV). Vitro experiments potential primarily attributed to miR-23a-3p, one most abundant MSC-sEV. Further, overexpression or knockdown revealed its target Krüppel-like factor 3 (KLF3) suppressed STAT3 signaling pathway high glucose (HG) induced HK-2 cells were essential renal-protective property Moreover, we found miR-23a-3p packaged into RNA Binding Motif Protein X-Linked (RBMX) transmitted HG-induced cells. Finally, inhibiting could mitigate effects db/db mice. These findings suggest systemic administration sEV derived from MSC, have capacity incorporate where they can exert against injury through delivery miR-23a-3p.

Язык: Английский

Процитировано

5
Hydrogels as Suitable miRNA Delivery Systems: A Review DOI Open Access
Haseena Makada, Moganavelli Singh

Polymers, Год журнала: 2025, Номер 17(7), С. 915 - 915

Опубликована: Март 28, 2025

The use of miRNA in therapeutics has, since its discovery 1993, attracted tremendous attention, and research this area has progressed rapidly. Since the advent RNA interference (RNAi), much about nucleic acid siRNA been elucidated. At same time, no miRNA-based drugs have passed phase II clinical trials. A significant obstacle to drug development is ease degradation relatively short half-life vivo miRNA. Hydrogels are networks cross-linked polymer chains with ability 'swell'. They shown remarkable capabilities that improve properties other researched carriers. In combination modification strategies inorganic carriers, hydrogel systems show promise for sustained delivery novel drugs. Although reported recently, focus predominantly on their wound-healing properties, a dearth information carrier abilities. This paper focuses more latest advancements developing hydrogels as system, emphasizing review will cover methods fabrication, efforts release, biomedical applications, future prospects.

Язык: Английский

Процитировано

0
Injectable Antibacterial Drug-Free Hydrogel Dressing Enabled by a Bioactive Peptide-Mimicking Synthetic Peptidyl Polymer DOI
Rong Zhang, Yongchang Tian, Jiaming Cui

и другие.

Acta Biomaterialia, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
3D mesenchymal stem cell exosome-functionalized hydrogels for corneal wound healing DOI

Yuehe Xu,

Chao Wei,

Li Ma

и другие.

Journal of Controlled Release, Год журнала: 2025, Номер 380, С. 630 - 646

Опубликована: Фев. 16, 2025

Язык: Английский

Процитировано

0
Extracellular vesicles and bioactive peptides for regenerative medicine in cosmetology DOI

Yize Yuan,

Kailu Cao,

Peifen Gao

и другие.

Ageing Research Reviews, Год журнала: 2025, Номер unknown, С. 102712 - 102712

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Different exosomes are loaded in hydrogels for the application in the field of tissue repair DOI Creative Commons
Yanyan Zhang, Wenjing Yan, Le Wu

и другие.

Frontiers in Bioengineering and Biotechnology, Год журнала: 2025, Номер 13

Опубликована: Март 3, 2025

Exosomes are double-membrane vesicular nanoparticles in the category of extracellular vesicles, ranging size from 30 to 150 nm, and released cells through a specific multi-step exocytosis process. have emerged as promising tools for tissue repair due their ability transfer bioactive molecules that promote cell proliferation, differentiation, regeneration. However, therapeutic application exosomes is hindered by rapid clearance body limited retention at injury site. To overcome these challenges, hydrogels, known high biocompatibility porous structure, been explored carriers exosomes. Hydrogels can provide controlled release mechanism, prolonging time targeted tissues, thus enhancing efficacy. This review focuses on combination different with hydrogels context repair. We first introduce sources functions exosomes, particularly those mesenchymal stem cells, roles regenerative medicine. then examine various types highlighting load Several strategies encapsulating discussed, including impact hydrogel composition structure exosome delivery efficiency. Finally, we applications exosomes-loaded such skin, bone, cartilage, nerve, explore challenges future directions this field. The offers significant promise advancing therapies.

Язык: Английский

Процитировано

0
Functionalized gelatin/poly(l-lactide-co-ε-caprolactone) fibrous membrane promotes scarless wound healing by modulating inflammation and reducing fibrosis DOI

Jing Wen,

Shue Jin, Xue Luo

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 306, С. 141785 - 141785

Опубликована: Март 5, 2025

Язык: Английский

Процитировано

0
Landscape of small nucleic acid therapeutics: moving from the bench to the clinic as next-generation medicines DOI Creative Commons
Mohan Liu, Yusi Wang, Yi-Bing Zhang

и другие.

Signal Transduction and Targeted Therapy, Год журнала: 2025, Номер 10(1)

Опубликована: Март 10, 2025

Abstract The ability of small nucleic acids to modulate gene expression via a range processes has been widely explored. Compared with conventional treatments, acid therapeutics have the potential achieve long-lasting or even curative effects editing. As result recent technological advances, efficient delivery for therapeutic and biomedical applications achieved, accelerating their clinical translation. Here, we review increasing number classes most common chemical modifications platforms. We also discuss key advances in design, development application each platform. Furthermore, this presents comprehensive profiles currently approved drugs, including 11 antisense oligonucleotides (ASOs), 2 aptamers 6 siRNA summarizing modifications, disease-specific mechanisms action strategies. Other candidates whose trial status recorded updated are discussed. consider strategic issues such as important safety considerations, novel vectors hurdles translating academic breakthroughs clinic. Small produced favorable results trials address previously “undruggable” targets, suggesting that they could be useful guiding additional candidates.

Язык: Английский

Процитировано

0