Molecular Biology Reports, Год журнала: 2025, Номер 52(1)
Опубликована: Май 28, 2025
Язык: Английский
Human Reproduction Update, Год журнала: 2023, Номер 29(6), С. 773 - 793
Опубликована: Июль 19, 2023
Abstract BACKGROUND Modern lifestyle has led to an increase in the age at conception. Advanced is one of critical risk factors for female-related infertility. It well known that maternal positively correlates with deterioration oocyte quality and chromosomal abnormalities oocytes embryos. The effect on endometrial function may be equally important factor influencing implantation rate, pregnancy overall female fertility. However, there are only a few published studies this topic, suggesting area been under-explored. Improving our knowledge aging from biological (cellular, molecular, histological) clinical perspectives would broaden understanding risks age-related OBJECTIVE AND RATIONALE objective narrative review critically evaluate existing literature focus synthesizing evidence impact conception success. This provide insights into gaps application research findings promote development treatment options field. SEARCH METHODS was prepared using PubMed (Medline) until February 2023 keywords such as ‘endometrial aging’, ‘receptivity’, ‘decidualization’, ‘hormone’, ‘senescence’, ‘cellular’, ‘molecular’, ‘methylation’, ‘biological age’, ‘epigenetic’, ‘oocyte recipient’, donation’, ‘embryo transfer’, ‘pregnancy rate’. Articles language other than English were excluded. OUTCOMES In endometrium, alterations occur cellular, histological levels negative biology impair receptivity. Additionally, advanced influences cellular senescence, which plays role during initial phase major obstacle suitable senolytic agents aging. Aging also accountable chronic conditions associated inflammaging, eventually can lead increased pro-inflammation tissue fibrosis. Furthermore, epigenetic regulation thus altering relation between its chronological age. donation cycles determine receptivity respect rates implantation, pregnancy, miscarriage, live birth have revealed contradictory inferences indicating need future mechanisms corresponding causal effects women’s WIDER IMPLICATIONS Increasing infertility IVF failures. Based complied observations synthesized conclusions review, shown functioning. information recommendations focusing molecular composition, transcriptomic changes further prospective needed explore newly emerging therapeutic options, target without affecting decidualization. Moreover, trial protocols, cycles, beneficial direct implications outcomes.
Язык: Английский
Процитировано
65
Nature Communications, Год журнала: 2024, Номер 15(1)
Опубликована: Янв. 31, 2024
Abstract Age-associated myometrial dysfunction can prompt complications during pregnancy and labor, which is one of the factors contributing to 7.8-fold increase in maternal mortality women over 40. Using single-cell/single-nucleus RNA sequencing spatial transcriptomics, we have constructed a cellular atlas aging myometrium from 186,120 cells across twenty perimenopausal postmenopausal women. We identify 23 cell subpopulations, including contractile venous capillary as well immune-modulated fibroblasts. Myometrial leads fewer cells, reduced level ion channel expression smooth muscle impaired gene endothelial, muscle, fibroblast, perivascular, immune cells. observe altered cell-to-cell communication an hallmark, associated with loss 25 signaling pathways, those related angiogenesis, tissue repair, contractility, immunity, nervous system regulation. These insights may contribute better understanding faced by older individuals labor.
Язык: Английский
Процитировано
9
Science China Life Sciences, Год журнала: 2025, Номер unknown
Опубликована: Апрель 11, 2025
Язык: Английский
Процитировано
1
Redox Biology, Год журнала: 2024, Номер 73, С. 103205 - 103205
Опубликована: Май 21, 2024
Hypoxia-inducible factor 1 alpha (HIF-1α) is a major molecular mediator of the hypoxic response. In endometrium, local conditions induced by hormonal fluctuations and endometrial vascular remodeling contribute to production HIF-1α, which plays an indispensable role in series physiological activities, such as menstruation metamorphosis. The sensitive regulation HIF-1α maintains cellular viability regenerative capacity endometrium against stresses hypoxia excess reactive oxygen species. contrast, abnormal levels exacerbate development various pathologies. This knowledge opens important possibilities for promising HIF-1α-centered strategies ameliorate disease. Nonetheless, additional efforts are required elucidate regulatory network promote applications human endometrium. Here, we summarize HIF-1α-mediated pathway physiology pathology, highlight latest treating diseases, improve receptivity.
Язык: Английский
Процитировано
6
International Journal of Molecular Sciences, Год журнала: 2023, Номер 25(1), С. 322 - 322
Опубликована: Дек. 25, 2023
Uterine aging is the process of senescence uterine tissue, observed in all middle-aged mammals. Since aging-related changes uterus are associated with infertility and poor pregnancy outcomes, a lack studies discussing aging, authors reviewed its consequences on reproduction. MEDLINE, Scopus, PubMed searches during years 1990–2023 were performed using combination keywords terms such topics. According to author’s evaluation, articles identified, selected, included this narrative review. The has an unfavorable impact There different selected molecular pathways related humans animals. impairs function myometrium, neurofibers human uterus, endometrium. These biological modulate oxidative stress, anti-inflammatory response, inflammation, mitochondrial function, DNA damage repair, etc. All these dysregulations have role poorer reproductive performance outcomes older most recent data suggest that accompanied by genetic, epigenetic, metabolic, immunological changes. negative mammalian species, but it could be potentially modulated pharmacological agents, as quercetin dasatinib.
Язык: Английский
Процитировано
12
The EMBO Journal, Год журнала: 2024, Номер unknown
Опубликована: Сен. 11, 2024
Abstract In preparation for a potential pregnancy, the endometrium of uterus changes into temporary structure called decidua. Senescent decidual stromal cells (DSCs) are enriched in decidua during decidualization, but underlying mechanisms this process remain unclear. Here, we performed single-cell RNA transcriptomics on ESCs and DSCs found that cell senescence decidualization is accompanied by increased levels branched-chain amino acid (BCAA) transporter SLC3A2. Depletion leucine, one acids, from cultured media decreased senescence, while high leucine diet resulted rates embryo loss mice. BCAAs induced via p38 MAPK pathway. contrast, TNFSF14+ natural killer (dNK) were to inhibit DSC interacting with its ligand TNFRSF14. As mice fed high-leucine diets, both NK depletion Tnfrsf14-deficient excessive uterine experienced adverse pregnancy outcomes. Further, SLC3A2-mediated BCAA intake, insufficient TNFRSF14 expression patients recurrent spontaneous abortion. summary, study suggests dNK maintain homeostasis TNFSF14/TNFRSF14, providing therapeutic strategy prevent senescence-associated
Язык: Английский
Процитировано
5
Materials Today Bio, Год журнала: 2024, Номер 26, С. 101045 - 101045
Опубликована: Апрель 3, 2024
The endometrium undergoes a series of precise monthly changes under the regulation dynamic levels ovarian hormones that are characterized by repeated shedding and subsequent regeneration without scarring. This provides potential for wound healing during endometrial injuries. Bioengineering materials highlight faithful replication constitutive cells extracellular matrix simulates physical biomechanical properties to larger extent. Significant progress has been made in this field, functional tissue bioengineering allows an in-depth investigation regulatory factors myometrial defects vitro highly therapeutic methods alleviate obstetric gynecological complications. However, much remains be learned about latest application technologies human endometrium. Here, we summarize existing developments biomaterials models improving female reproductive potential.
Язык: Английский
Процитировано
4
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(9), С. 4680 - 4680
Опубликована: Апрель 25, 2024
The decline in female fecundity is linked to advancing chronological age. ovarian reserve diminishes quantity and quality as women age, impacting reproductive efficiency the aging process rest of body. NAD+ an essential coenzyme cellular energy production, metabolism, cell signaling, survival. It involved various age-related conditions. Hallmarks associated with aging, diseases, metabolic dysfunctions can significantly affect fertility by disturbing delicate relationship between metabolism reproduction. Enzymes such sirtuins, PARPs, CD38 play roles biology, which actively consume their enzymatic activities. In recent years, has gained much attention for its role diseases like cancer, Alzheimer’s, cardiovascular neurodegenerative disorders, highlighting involvement pathophysiological processes. However, impact on reproduction not well understood. This review aims bridge this knowledge gap comprehensively exploring complex interplay biology providing valuable information that could help develop plans improve women’s health prevent issues.
Язык: Английский
Процитировано
4
Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(45)
Опубликована: Окт. 29, 2024
The human uterus is a complex and dynamic organ whose lining grows, remodels, regenerates every menstrual cycle or upon tissue damage. Here, we applied single-cell RNA sequencing to profile more the 50,000 uterine cells from both endometrium myometrium of five healthy premenopausal individuals, jointly analyzed data with previously published dataset 15 subjects. resulting normal cell atlas contains than 167K cells, representing lymphatic endothelium, blood stromal, ciliated epithelium, unciliated immune populations. Focused analyses within each major type comparisons subtype labels prior studies allowed us document supporting evidence, resolve naming conflicts, propose consensus annotation system 39 subtypes. We release their gene expression centroids, differentially expressed genes, messenger Ribonucleic Acid (mRNA) patterns literature-based markers as shared community resource. identify multiple potential progenitor cells: compartment-wide progenitors for cross-lineage multipotent stromal that may replenish epithelial, endothelial compartments. Furthermore, many types subtypes exhibit shifts in number transcriptomes across different phases cycle. Finally, between premenopausal, postpartum, postmenopausal samples revealed substantial alterations composition, particularly proportions endothelial, cells. taxonomy molecular report here are expected inform basic biology function its disorders.
Язык: Английский
Процитировано
4
Phytomedicine, Год журнала: 2024, Номер 136, С. 156288 - 156288
Опубликована: Ноя. 25, 2024
Язык: Английский
Процитировано
4