Cobra Venom: From Envenomation Syndromes to Therapeutic Innovations
International Journal of Peptide Research and Therapeutics,
Год журнала:
2024,
Номер
30(6)
Опубликована: Окт. 4, 2024
Язык: Английский
Exploring snake venoms beyond the primary sequence: From proteoforms to protein-protein interactions
Toxicon,
Год журнала:
2024,
Номер
247, С. 107841 - 107841
Опубликована: Июнь 29, 2024
Snakebite
envenomation
has
been
a
long-standing
global
issue
that
is
difficult
to
treat,
largely
owing
the
flawed
nature
of
current
immunoglobulin-based
antivenom
therapy
and
complexity
snake
venoms
as
sophisticated
mixtures
bioactive
proteins
peptides.
Comprehensive
characterisation
venom
compositions
essential
better
understanding
toxicity
inform
effective
rationally
designed
antivenoms.
Additionally,
greater
composition
will
likely
unearth
novel
biologically
active
peptides
have
promising
therapeutic
or
biotechnological
applications.
While
bottom-up
proteomic
workflow
main
approach
for
cataloguing
at
toxin
family
level,
it
unable
capture
heterogeneity
in
form
protein
isoforms
higher-order
interactions
are
important
driving
but
remain
underexplored.
This
review
aims
highlight
importance
beyond
primary
sequence,
post-translational
modifications
give
rise
different
proteoforms
myriad
complexes
venoms.
We
focus
on
top-down
workflows
identify
further
discuss
alternative
separation,
instrumentation,
data
processing
strategies
may
improve
proteoform
identification.
The
structural
techniques
implemented
also
discussed;
we
emphasise
need
complementary
higher
resolution
bioanalytical
such
mass
spectrometry-based
approaches,
X-ray
crystallography
cryogenic
electron
microscopy,
elucidate
poorly
characterised
tertiary
quaternary
structures.
envisage
expansion
"toolbox"
with
proteomics
high-resolution
structure
determination
be
pivotal
advancing
towards
development
improved
biotechnology
Язык: Английский
Comparison of the intrageneric neutralization scope of monospecific, bispecific/monogeneric and polyspecific/monogeneric antisera raised in horses immunized with sub-Saharan African snake venoms
PLoS neglected tropical diseases,
Год журнала:
2024,
Номер
18(5), С. e0012187 - e0012187
Опубликована: Май 29, 2024
Background
Snakebite
envenomation
inflicts
a
high
burden
of
mortality
and
morbidity
in
sub-Saharan
Africa.
Antivenoms
are
the
mainstay
therapy
envenomation,
there
is
an
urgent
need
to
develop
antivenoms
broad
neutralizing
efficacy
for
this
region.
The
venoms
used
as
immunogens
manufacture
snake
normally
selected
considering
their
medical
importance
availability.
Additionally,
ability
induce
antibody
responses
with
capability
should
be
considered,
issue
that
involves
immunization
scheme
animal
species
being
immunized.
Methodology/Principal
findings
Using
lethality
neutralization
assay
mice,
we
compared
intrageneric
scope
antisera
generated
by
horses
monospecific,
bispecific/monogeneric,
polyspecific/monogeneric
formulated
Bitis
spp.,
Echis
Dendroaspis
spitting
Naja
spp.
or
non-spitting
It
was
found
raised
all
were
able
neutralize
homologous
and,
single
exception,
heterologous
congeneric
(considering
separately).
In
general,
polyspecific
spp,
spp
gave
best
profile
against
these
genera.
For
venoms,
no
significant
differences
between
bispecific
antisera.
A
similar
result
obtained
case
except
antiserum
more
effective
N
.
melanoleuca
nivea
monospecific
antiserum.
Conclusions/Significance
use
alternative
produce
monogeneric
wide
coverage
African
snakes
,
(non-spitting)
On
other
hand,
immunogen
composed
venom
nigricollis
suitable
antivenom
These
can
design
Язык: Английский
Exploring Top-Down Mass Spectrometric Approaches To Probe Forest Cobra (Naja melanoleuca) Venom Proteoforms
Journal of Proteome Research,
Год журнала:
2024,
Номер
23(10), С. 4601 - 4613
Опубликована: Сен. 4, 2024
Snake
venoms
are
comprised
of
bioactive
proteins
and
peptides
that
facilitate
severe
snakebite
envenomation
symptoms.
A
comprehensive
understanding
venom
compositions
the
subtle
heterogeneity
therein
is
important.
While
bottom-up
proteomics
has
been
well-established
approach
to
catalogue
compositions,
top-down
emerged
as
a
complementary
strategy
characterize
at
intact
protein
level.
However,
not
widely
implemented
in
snake
field
proteomics,
with
various
emerging
methods
yet
be
developed
for
systems.
Here,
we
have
explored
three
main
mass
spectrometry
methodologies
proof-of-concept
study
selected
three-finger
toxin
phospholipase
Язык: Английский
Snakebites in Cameroon by Species Whose Effects Are Poorly Described
Tropical Medicine and Infectious Disease,
Год журнала:
2024,
Номер
9(12), С. 300 - 300
Опубликована: Дек. 6, 2024
Snakes
responsible
for
bites
are
rarely
identified,
resulting
in
a
loss
of
information
about
snakebites
from
venomous
species
whose
venom
effects
poorly
understood.
A
prospective
clinical
study
including
patients
bitten
by
snake
was
conducted
Cameroon
between
2019
and
2021
to
evaluate
the
efficacy
tolerability
marketed
polyvalent
antivenom.
Clinical
presentation
during
first
3
days
hospitalization
recorded
following
standardized
protocol.
This
ancillary
aimed
assess
frequency
different
encountered
describe
symptoms
formally
identified
species.
Of
447
included
study,
159
(35.6%)
brought
that
caused
bite
specialist.
Out
these,
8
specimens
could
not
be
due
poor
condition,
19
were
non-venomous
species,
95
belonged
Echis
romani—formerly
E.
ocellatus—species.
The
remaining
37
2
Atheris
squamigera,
12
Atractaspis
spp.,
Bitis
arietans,
11
Causus
maculatus,
1
Dendroaspis
jamesoni,
Naja
haje,
N.
katiensis,
5
melanoleuca
complex,
nigricollis.
Symptoms,
severity
envenomation,
post-treatment
course
described.
Symptoms
consistent
with
cases
described
literature,
but
some
specific
features
highlighted.
Язык: Английский
Streamlining the Analysis of Proteins from Snake Venom
Journal of Proteome Research,
Год журнала:
2024,
Номер
23(7), С. 2367 - 2375
Опубликована: Май 30, 2024
Investigating
snake
venom
is
necessary
for
developing
new
treatments
envenoming
and
harnessing
the
therapeutic
potential
that
lies
within
toxins.
Despite
considerable
efforts
in
previous
research,
several
technical
challenges
remain
characterizing
individual
components
such
complex
mixtures.
Here,
we
present
native
top-down
mass
spectrometry
(MS)
workflows
enable
analysis
of
proteins
mixtures
showcase
utility
these
methodologies
on
King
cobra
(
Язык: Английский
A consensus recombinant elapid long-chain α-neurotoxin and how protein folding matters for antibody recognition and neutralization of elapid venoms
Biochemical and Biophysical Research Communications,
Год журнала:
2024,
Номер
732, С. 150420 - 150420
Опубликована: Июль 18, 2024
Язык: Английский
Interrogation of three-finger toxin and phospholipase A2 higher order structures from the forest cobra (Naja melanoleuca) venom using a mass spectrometric approach
International Journal of Mass Spectrometry,
Год журнала:
2024,
Номер
unknown, С. 117346 - 117346
Опубликована: Сен. 1, 2024
Язык: Английский