Machine Learning Technology for EEG-Forecast of the Blood–Brain Barrier Leakage and the Activation of the Brain’s Drainage System during Isoflurane Anesthesia DOI Creative Commons
Oxana Semyachkina‐Glushkovskaya, Konstantin Sergeev, Nadezhda Semenova

и другие.

Biomolecules, Год журнала: 2023, Номер 13(11), С. 1605 - 1605

Опубликована: Ноя. 2, 2023

Anesthesia enables the painless performance of complex surgical procedures. However, effects anesthesia on brain may not be limited only by its duration. Also, anesthetic agents cause long-lasting changes in brain. There is growing evidence that can disrupt integrity blood-brain barrier (BBB), leading to neuroinflammation and neurotoxicity. there are no widely used methods for real-time BBB monitoring during surgery. The development technologies an express diagnosis opening (OBBB) a challenge reducing post-surgical/anesthesia consequences. In this study male rats, we demonstrate successful application machine learning technology, such as artificial neural networks (ANNs), recognize OBBB induced isoflurane, which ANNs were trained our previously presented data obtained sound-induced with 85% testing accuracy. Using optical nonlinear analysis OBBB, found 1% isoflurane does induce any BBB, while 4% caused significant leakage all tested rats. Both stimulate brain's drainage system (BDS) dose-related manner. We show 57% rats BDS activation 81% These results open new perspectives clinically bedside EEG-monitoring BDS.

Язык: Английский

The Role of Glymphatic System in Alzheimer’s and Parkinson’s Disease Pathogenesis DOI Creative Commons

Francesca R. Buccellato,

Marianna D’Anca, María Serpente

и другие.

Biomedicines, Год журнала: 2022, Номер 10(9), С. 2261 - 2261

Опубликована: Сен. 13, 2022

Alzheimer's disease (AD) is the most common cause of neurodegenerative dementia, whilst Parkinson's (PD) a movement disorder. These two disorders share accumulation toxic proteins as pathological hallmark. The lack definitive disease-modifying treatments for these neurogenerative diseases has led to hypothesis new pathogenic mechanisms target and design potential therapeutic approaches. recent observation that glymphatic system supposed be responsible cerebrospinal fluid into brain clearance metabolic waste study its involvement in pathogenesis classic proteinopathies. Aquaporin-4 (AQP4), water channel located endfeet astrocyte membrane, considered primary driver system, defective AQP4-mediated drainage been linked objective present review body knowledge links AD PD other lifestyle factors such sleep deprivation exercise may influence function. We will also focus on neuroimaging approaches could identify marker detect changes.

Язык: Английский

Процитировано

83
The glymphatic system and Amyotrophic lateral sclerosis DOI
Andrew Eisen, Maiken Nedergaard,

E. G. Gray

и другие.

Progress in Neurobiology, Год журнала: 2024, Номер 234, С. 102571 - 102571

Опубликована: Янв. 22, 2024

Язык: Английский

Процитировано

10
Neutrophil extracellular trap-mediated impairment of meningeal lymphatic drainage exacerbates secondary hydrocephalus after intraventricular hemorrhage DOI Creative Commons
Qiang Zhang, Yujie Chen,

Yingpei Li

и другие.

Theranostics, Год журнала: 2024, Номер 14(5), С. 1909 - 1938

Опубликована: Янв. 1, 2024

Rationale: Hydrocephalus is a substantial complication after intracerebral hemorrhage (ICH) or intraventricular (IVH) that leads to impaired cerebrospinal fluid (CSF) circulation.Recently, brain meningeal lymphatic vessels (mLVs) were shown serve as critical drainage pathways for CSF.Our previous studies indicated the degradation of neutrophil extracellular traps (NETs) ICH/IVH alleviates hydrocephalus.However, mechanisms by which NET exerts beneficial effects in hydrocephalus remain unclear.Methods: A mouse model following IVH was established infusing autologous blood into both wildtype and Cx3cr1 -/-mice.By studying features processes model, we investigated contribution mLVs NETs development progression secondary IVH.Results: This study observed widespread presence neutrophils, fibrin IVH, alleviated injury.Importantly, improved CSF enhancing recovery endothelial cells (LECs).Furthermore, our showed activated membrane protein CX3CR1 on LECs IVH.In contrast, repair promoted ameliorated knockdown -/-mice.Conclusion: Our findings participate injury contribute acute LEC thrombosis.CX3CR1 key molecule NET-induced damage thrombosis, mLV dysfunction exacerbates injury.NETs may be target preventing obstruction IVH.

Язык: Английский

Процитировано

10
New perspectives on the glymphatic system and the relationship between glymphatic system and neurodegenerative diseases DOI Creative Commons
Yujie Sun,

Qiankun Lv,

Junyi Liu

и другие.

Neurobiology of Disease, Год журнала: 2025, Номер 205, С. 106791 - 106791

Опубликована: Янв. 6, 2025

Neurodegenerative diseases (ND) are characterized by the accumulation of aggregated proteins. The glymphatic system, through its rapid exchange mechanisms between cerebrospinal fluid (CSF) and interstitial (ISF), facilitates movement metabolic substances within brain, serving functions akin to those peripheral lymphatic system. This emerging waste clearance mechanism offers a novel perspective on removal pathological in ND. article elucidates recent discoveries regarding system updates relevant concepts model. It discusses potential roles ND, including Alzheimer's disease (AD), Parkinson's (PD), multiple atrophy (MSA), proposes as therapeutic target for these conditions.

Язык: Английский

Процитировано

2
Impaired Meningeal Lymphatics and Glymphatic Pathway in Patients with White Matter Hyperintensity DOI Creative Commons
Ying Zhou,

Rui Xue,

Yifei Li

и другие.

Advanced Science, Год журнала: 2024, Номер 11(26)

Опубликована: Май 5, 2024

Abstract White matter hyperintensity (WMH) represents a critical global medical concern linked to cognitive decline and dementia, yet its underlying mechanisms remain poorly understood. Here, humans are directly demonstrated that high WMH burden correlates with delayed drainage of meningeal lymphatic vessels (mLVs) glymphatic pathway. Additionally, longitudinal cohort study reveals dysfunction predicts progression. Next, in rat model WMH, the presence impaired lymphangiogenesis is confirmed, followed by elevated microglial activation white demyelination. Notably, enhancing through adeno‐associated virus delivery vascular endothelial growth factor‐C (VEGF‐C) mitigates gliosis Conversely, blocking mLVs VEGF‐C trap strategy exacerbates these changes. The findings highlight role pathway aggravating brain injury, providing potential novel for prevention treatment.

Язык: Английский

Процитировано

7
Astrocytes in aging DOI Creative Commons
Lara Labarta-Bajo, Nicola J. Allen

Neuron, Год журнала: 2025, Номер 113(1), С. 109 - 126

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

1
Overview of the meningeal lymphatic vessels in aging and central nervous system disorders DOI Creative Commons
Huimin Jiang, Huimin Wei, Yifan Zhou

и другие.

Cell & Bioscience, Год журнала: 2022, Номер 12(1)

Опубликована: Дек. 17, 2022

Abstract In the aging process and central nervous system (CNS) diseases, functions of meningeal lymphatic vessels (MLVs) are impaired. Alterations in MLVs have been observed aging-related neurodegenerative brain tumors, even cerebrovascular disease. These findings reveal a new perspective on CNS disorders provide promising therapeutic target. Additionally, recent neuropathological studies shown that exchange soluble components between cerebrospinal fluid (CSF) interstitial (ISF) drain metabolites, cellular debris, misfolded proteins, immune cells from CSF into deep cervical lymph nodes (dCLNs), directly connecting with peripheral circulation. Impairment dysfunction lymphatics can lead to accumulation toxic proteins brain, exacerbating progression neurological disorders. However, for many causal relationship changes is not fully clear. Here, after brief historical retrospection, we review discoveries about hallmarks their roles as well potential targets treatment neurologic diseases.

Язык: Английский

Процитировано

25
Brain Vascular Health in ALS Is Mediated through Motor Cortex Microvascular Integrity DOI Creative Commons
Stefanie Schreiber, José Bernal, Philipp Arndt

и другие.

Cells, Год журнала: 2023, Номер 12(6), С. 957 - 957

Опубликована: Март 21, 2023

Brain vascular health appears to be critical for preventing the development of amyotrophic lateral sclerosis (ALS) and slowing its progression. ALS patients often demonstrate cardiovascular risk factors commonly suffer from cerebrovascular disease, with evidence pathological alterations in their small cerebral blood vessels. Impaired brain has detrimental effects on motor neurons: endothelial growth factor levels are lowered ALS, which can compromise cell formation integrity blood–brain barrier. Increased turnover neurovascular unit cells precedes senescence, which, together pericyte alterations, further fosters failure toxic metabolite removal. We here provide a comprehensive overview pathogenesis impaired how novel magnetic resonance imaging techniques aid detection. In particular, we discuss patterns supply cortex number branches anterior middle arteries acting as marker resistance resilience against downstream events ALS. outline certain interventions adapted patient needs capabilities have potential mechanistically target microvasculature towards favorable patterns. Through this strategy, aim guide approaches management better understanding pathophysiology.

Язык: Английский

Процитировано

17
Age-Related Alterations in Immune Function and Inflammation: Focus on Ischemic Stroke DOI Creative Commons

Qiuxin Chen,

Minmin Wu, Qiang Tang

и другие.

Aging and Disease, Год журнала: 2023, Номер unknown

Опубликована: Янв. 1, 2023

The aging of the global population poses significant scientific challenges. Moreover, biological process is most risk factor for chronic illnesses; therefore, understanding molecular and cellular mechanisms underlying these aging-related challenges crucial extending healthy lifespan older individuals. Preventing brain remains a priority public health goal, integrative comprehensive analyses have revealed that immunosenescence potential cause age-related damage disease (e.g., stroke). Importantly, neuroinflammatory immune systems present two-way contact thus can affect each other. Emerging evidence supports numerous effects immunosenescence- inflammation-mediated immunity in neurologically injured brains. In this study, we briefly outline how alters pathophysiology transcriptional amplitude patients who experienced stroke then discuss system its components are affected by age after stroke. Finally, highlight emerging interventions with to slow down or reduce prevent onset.

Язык: Английский

Процитировано

12
Super‐Resolution Stimulated Raman Scattering Microscopy with Graphical User Interface–Supported A‐PoD DOI Creative Commons

Hongje Jang,

Yajuan Li, Shuang Wu

и другие.

Current Protocols, Год журнала: 2024, Номер 4(1)

Опубликована: Янв. 1, 2024

Abstract Raman microscopy is a vibrational imaging technology that can detect molecular chemical bond signals. Since this signal originated from almost every mode of molecules with different energy levels, it provides spatiotemporal distribution various in living organisms without the need for any labeling. The limitations low strength have been effectively addressed by incorporating stimulated emission process, leading to development scattering (SRS) microscopy. Furthermore, issue spatial resolution has resolved through application computational techniques, specifically image deconvolution. In article, we present comprehensive guide super‐resolution SRS using an Adam‐based pointillism deconvolution (A‐PoD) algorithm, complemented user‐friendly graphical user interface (GUI). We delve into crucial parameters and conditions necessary achieving super‐resolved images imaging. Additionally, provide step‐by‐step walkthrough preprocessing steps use GUI‐supported A‐PoD. This complete package offers platform microscopy, enhancing versatility applicability advanced technique reveal nanoscopic multimolecular nature. © 2024 Authors. Current Protocols published Wiley Periodicals LLC. Basic Protocol : Super‐resolution interface–supported A‐PoD Support Deuterium labeling on cells heavy water metabolic

Язык: Английский

Процитировано

3