circSAMD4A participates in the apoptosis and autophagy of dopaminergic neurons via the miR‑29c‑3p‑mediated AMPK/mTOR pathway in Parkinson's disease DOI Creative Commons
Wensheng Wang,

Rongxiang Lv,

Jingjing Zhang

и другие.

Molecular Medicine Reports, Год журнала: 2021, Номер 24(1)

Опубликована: Май 27, 2021

Parkinson's disease (PD) can lead to movement injury and cognitive dysfunction. Although advances have been made in attenuating PD, the effect of inhibiting development PD remains disappointing. Therefore, present study aimed at investigating etiology developing an alternative therapeutic strategy for patients with PD. A mouse model was established using intraperitoneal injection 1‑methyl‑4‑phenyl‑1,2,3,6‑tetrahydropyridine hydrochloride (MPTP‑HCl; 30 mg/kg/day 5 days), a cellular by treating SH‑SY5Y cells different concentrations 1‑methyl‑4‑phenylpyridinium (MPP+) 24 h. The expression levels circular RNA sterile α motif domain containing 4A (circSAMD4A) microRNA (miR)‑29c‑3p both midbrain tissues were detected via reverse transcription‑quantitative PCR. interaction between circSAMD4A miR‑29c‑3p verified dual‑luciferase reporter experiment. Apoptosis‑, autophagy‑ 5'AMP‑activated protein kinase (AMPK)/mTOR cascade‑associated proteins western blotting. Furthermore, TUNEL staining flow cytometry used analyze cell apoptosis. It found that upregulated, while downregulated animal models. Moreover, directly targeted negatively regulated miR‑29c‑3p. Further studies identified knockdown inhibited MPTP‑ or MPP+‑induced apoptosis autophagy; however, these effects abolished inhibitor. In addition, repressed phosphorylated‑AMPK increased mTOR models, which reversed Collectively, results suggested participated autophagy dopaminergic neurons modulating AMPK/mTOR cascade

Язык: Английский

Targeting circular RNAs as a therapeutic approach: current strategies and challenges DOI Creative Commons
Alina T. He, Jinglei Liu, Feiya Li

и другие.

Signal Transduction and Targeted Therapy, Год журнала: 2021, Номер 6(1)

Опубликована: Май 21, 2021

Abstract Significant progress has been made in circular RNA (circRNA) research recent years. Increasing evidence suggests that circRNAs play important roles many cellular processes, and their dysregulation is implicated the pathogenesis of various diseases. CircRNAs are highly stable usually expressed a tissue- or cell type-specific manner. Therefore, they currently being explored as potential therapeutic targets. Gain-of-function loss-of-function approaches typically performed using circRNA expression plasmids interference-based strategies, respectively. These strategies have limitations can be mitigated nanoparticle exosome delivery systems. Furthermore, developments show cre-lox system used to knockdown cell-specific While still early stages development, CRISPR/Cas13 shown promise knocking down with high specificity efficiency. In this review, we describe properties functions highlight significance disease. We summarize overexpress approach. Lastly, discuss major challenges propose future directions for development circRNA-based therapeutics.

Язык: Английский

Процитировано

373
Autophagy‐associated non‐coding RNAs: Unraveling their impact on Parkinson's disease pathogenesis DOI Creative Commons
Md Sadique Hussain, Ehssan Moglad, Muhammad Afzal

и другие.

CNS Neuroscience & Therapeutics, Год журнала: 2024, Номер 30(5)

Опубликована: Май 1, 2024

Parkinson's disease (PD) is a degenerative neurological condition marked by the gradual loss of dopaminergic neurons in substantia nigra pars compacta. The precise etiology PD remains unclear, but emerging evidence suggests significant role for disrupted autophagy-a crucial cellular process maintaining protein and organelle integrity.

Язык: Английский

Процитировано

18
Autophagy and apoptosis cascade: which is more prominent in neuronal death? DOI

Rohan Gupta,

Rashmi K. Ambasta, Pravir Kumar

и другие.

Cellular and Molecular Life Sciences, Год журнала: 2021, Номер 78(24), С. 8001 - 8047

Опубликована: Ноя. 6, 2021

Язык: Английский

Процитировано

101
Competing Endogenous RNA Networks as Biomarkers in Neurodegenerative Diseases DOI Open Access
Leticia Moreno‐García, Tresa López‐Royo, Ana Cristina Calvo

и другие.

International Journal of Molecular Sciences, Год журнала: 2020, Номер 21(24), С. 9582 - 9582

Опубликована: Дек. 16, 2020

Protein aggregation is classically considered the main cause of neuronal death in neurodegenerative diseases (NDDs). However, increasing evidence suggests that alteration RNA metabolism a key factor etiopathogenesis these complex disorders. Non-coding RNAs are major contributor to human transcriptome and particularly abundant central nervous system, where they have been proposed be involved onset development NDDs. Interestingly, some ncRNAs (such as lncRNAs, circRNAs pseudogenes) share common functionality their ability regulate gene expression by modulating miRNAs phenomenon known competing endogenous mechanism. Moreover, found body fluids presence concentration could serve potential non-invasive biomarkers In this review, we summarize ceRNA networks described Alzheimer’s disease, Parkinson’s multiple sclerosis, amyotrophic lateral sclerosis spinocerebellar ataxia type 7, discuss Although numerous studies carried out, further research needed validate interactions between alterations editing provide specific ceRNET profiles for disorders, paving way better understanding diseases.

Язык: Английский

Процитировано

94
Circular RNAs: Promising Molecular Biomarkers of Human Aging-Related Diseases via Functioning as an miRNA Sponge DOI Creative Commons
Shuyue Ren,

Peirong Lin,

Jingrong Wang

и другие.

Molecular Therapy — Methods & Clinical Development, Год журнала: 2020, Номер 18, С. 215 - 229

Опубликована: Июнь 6, 2020

Circular RNAs (circRNAs) are a new class of noncoding single-stranded that differ from linear microRNAs (miRNAs), since they form covalently closed loop structures without free 3' poly(A) tails or 5' caps. circRNAs the competitive endogenous (ceRNAs) by binding to miRNA through response elements (MREs) (i.e., "miRNA sponge"), thereby reducing quantity available target mRNA, subsequently promoting mRNA stability protein expression, which involves initiation and progress human diseases. Owing these features abundance, stability, conservative property, tissue stage specificity, widely distributing in extracellular space various bodily fluids, can be considered as potential biomarkers for Here, we reviewed promising being disease biomarkers, focused on their regulatory function acting sponges, described roles cancer, cardiovascular neurodegenerative diseases, osteoarthritis, rheumatoid arthritis, diabetes, other aging-related provide direction pathogenesis, diagnosis, treatment

Язык: Английский

Процитировано

84
The influence of circular RNAs on autophagy and disease progression DOI Open Access
Yian Wang,

Yongzhen Mo,

Peng Miao

и другие.

Autophagy, Год журнала: 2021, Номер 18(2), С. 240 - 253

Опубликована: Апрель 27, 2021

Circular RNAs (circRNAs) are non-coding that have attracted considerable attention in recent years. Owing to their distinct circular structure, circRNAs stable cells. Autophagy is a catabolic process helps the degradation and recycling of harmful or inessential biological macromolecules cells enables adapt stress changes internal external environments. Evidence has shown influence course disease by regulating autophagy, which indicates autophagy involved onset development various diseases can affect drug resistance (for example, it affects cisplatin tumors). In this review, we summarized role on progression as well resistance. The review will expand our understanding tumors cardiovascular neurological also suggest novel therapeutic strategies.Abbreviations: ACR: autophagy-related circRNA; ADSCs: adipogenic mesenchymal stem cells; AMPK: AMP-activated protein kinase; ATG: related; BCL2: BCL2 apoptosis regulator; BECN1: beclin 1; ceRNA: competing endogenous RNA; circRNA: CMA: chaperone-mediated autophagy; EPCs: endothelial progenitor LE/MVBs: late endosomes/multivesicular bodies; MAP1LC3/LC3: microtubule associated 1 light chain 3; MTOR: mechanistic target rapamycin NSCLC: non-small cell lung cancer; PDLSCs: periodontal ligament PE: phosphatidylethanolamine; PtdIns: phosphatidylinositol; PtdIns3K: phosphatidylinositol 3-kinase; PtdIns3P: phosphatidylinositol-3-phosphate 1,2-dipalmitoyl; PTEN: phosphatase tensin homolog; RBPs: RNA-binding proteins; SiO2: silicon dioxide; TFEB: transcription factor EB; ULK: unc-51 like activating kinase 1.

Язык: Английский

Процитировано

70
Epigenetic Regulation of Neuroinflammation in Parkinson’s Disease DOI Open Access
Madiha Rasheed, Junhan Liang, Chaolei Wang

и другие.

International Journal of Molecular Sciences, Год журнала: 2021, Номер 22(9), С. 4956 - 4956

Опубликована: Май 7, 2021

Neuroinflammation is one of the most significant factors involved in initiation and progression Parkinson’s disease. PD a neurodegenerative disorder with motor disability linked various complex diversified risk factors. These trigger myriads cellular molecular processes, such as misfolding defective proteins, oxidative stress, mitochondrial dysfunction, neurotoxic substances that induce selective neurodegeneration dopamine neurons. This neuronal damage activates immune system, including glial cells inflammatory cytokines, to neuroinflammation. The transition acute chronic neuroinflammation enhances susceptibility inflammation-induced dopaminergic neuron damage, forming vicious cycle prompting an individual development. Epigenetic mechanisms recently have been at forefront regulation neuroinflammatory PD, proposing new dawn for breaking this cycle. review examined core epigenetic activation phenotypic transformation mediated PD. We found do not work independently, despite being coordinated each other activate pathways. In regard, we attempted find synergic correlation contribution these modifications pathways broaden canvas underlying pathological Moreover, study highlighted dual characteristics (neuroprotective/neurotoxic) marks, which may counteract pathogenesis make them potential candidates devising future diagnosis treatment.

Язык: Английский

Процитировано

59
CircSV2b participates in oxidative stress regulation through miR-5107-5p-Foxk1-Akt1 axis in Parkinson's disease DOI Creative Commons
Quancheng Cheng, Jianwei Wang, Man Li

и другие.

Redox Biology, Год журнала: 2022, Номер 56, С. 102430 - 102430

Опубликована: Авг. 12, 2022

As a novel type of non-coding RNAs, covalently closed circular RNAs (circRNAs) are ubiquitously expressed in eukaryotes. Emerging studies have indicated that dysregulation circRNAs was related to neurological diseases. However, the biogenesis, regulation, function, and mechanism Parkinson's disease (PD) remain largely unclear. In this study, thirty-three differentially (DECs) were detected by RNA-sequencing between MPTP-induced PD mice model wild-type mice. Quantitative real-time PCR used determine RNA level DECs striatum (STR), substantia nigra pars compacta (SNpc), serum exosomes, it found circSV2b downregulated Then, functional experiments vivo employed explore effect PD. For dual-luciferase reporter, fluorescence situ hybridization (FISH), immunoprecipitation (RIP), pull-down, gene editing, CUT & Tag performed vitro confirm directly sponged miR-5107-5p alleviated suppression expression target Foxk1, then positively regulated Akt1 transcription. vivo, mechanistic analysis demonstrated overexpression resisted oxidative stress damage through ceRNA-Akt1 axis models. Taken together, these findings suggested miR-5107-5p-Foxk1-Akt1 might serve as key treatment, highlighted significant change exosomes. Therefore, be biomarker for diagnosis treatment

Язык: Английский

Процитировано

47
Recent insights into the roles of circular RNAs in human brain development and neurologic diseases DOI
Sajad Najafi, Seyed Mohsen Aghaei Zarch, Jamal Majidpoor

и другие.

International Journal of Biological Macromolecules, Год журнала: 2022, Номер 225, С. 1038 - 1048

Опубликована: Ноя. 18, 2022

Язык: Английский

Процитировано

42
CircEPS15, as a sponge of MIR24-3p ameliorates neuronal damage in Parkinson disease through boosting PINK1-PRKN-mediated mitophagy DOI
Yuanzhang Zhou, Yang Liu,

Zhengwei Kang

и другие.

Autophagy, Год журнала: 2023, Номер 19(9), С. 2520 - 2537

Опубликована: Апрель 4, 2023

Despite growing evidence that has declared the importance of circRNAs in neurodegenerative diseases, clinical significance dopaminergic (DA) neuronal degeneration pathogenesis Parkinson disease (PD) remains unclear. Here, we performed rRNA-depleted RNA sequencing and detected more than 10,000 plasma samples PD patients. In consideration ROC correlation between Hohen-Yahr stage (H-Y stage) Unified Disease Rating Scale-motor score (UPDRS) 40 patients,

Язык: Английский

Процитировано

29