Exposure to polystyrene nanoplastics impairs sperm metabolism and pre-implantation embryo development in mice DOI Creative Commons
Yingdong Liu,

Fengdan Hao,

Hao Liang

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2025, Номер 13

Опубликована: Фев. 28, 2025

Introduction Microplastics and nanoplastics are prevalent environmental contaminants. Recent reports indicate that polystyrene nanoparticles may adversely impact male reproductive health. This study aims to examine the effects of exposure on sperm metabolism development pre-implantation embryos. Methods In this study, C57BL/6 mice were orally gavage-administered (60 nm, 20 mg/kg/day) for 35 days assess their reproduction early embryonic development. Experiments included testicular transcriptome analysis, metabolomics, motility fertilization assays, ROS detection, RNA sequencing 2-cell embryos, revealing adverse embryo Results The results revealed oral gavage induced a pronounced immune-inflammatory response in tissue, reduced motility, significantly lowered rate. Notably, from treated exhibited substantial metabolic disruptions, affecting key pathways, including glycerophospholipid biosynthesis DNA repair. After fertilization, embryos at stage suffered damage apoptotic repair subsequently impairing Discussion conclusion, demonstrated administration affects function, resulting abnormalities alterations associated gene expression profiles. These findings offer essential scientific insights future research into sperm-mediated transgenerational

Язык: Английский

Exposure to polystyrene nanoplastics impairs sperm metabolism and pre-implantation embryo development in mice DOI Creative Commons
Yingdong Liu,

Fengdan Hao,

Hao Liang

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2025, Номер 13

Опубликована: Фев. 28, 2025

Introduction Microplastics and nanoplastics are prevalent environmental contaminants. Recent reports indicate that polystyrene nanoparticles may adversely impact male reproductive health. This study aims to examine the effects of exposure on sperm metabolism development pre-implantation embryos. Methods In this study, C57BL/6 mice were orally gavage-administered (60 nm, 20 mg/kg/day) for 35 days assess their reproduction early embryonic development. Experiments included testicular transcriptome analysis, metabolomics, motility fertilization assays, ROS detection, RNA sequencing 2-cell embryos, revealing adverse embryo Results The results revealed oral gavage induced a pronounced immune-inflammatory response in tissue, reduced motility, significantly lowered rate. Notably, from treated exhibited substantial metabolic disruptions, affecting key pathways, including glycerophospholipid biosynthesis DNA repair. After fertilization, embryos at stage suffered damage apoptotic repair subsequently impairing Discussion conclusion, demonstrated administration affects function, resulting abnormalities alterations associated gene expression profiles. These findings offer essential scientific insights future research into sperm-mediated transgenerational

Язык: Английский

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