Exposure to polystyrene nanoplastics impairs sperm metabolism and pre-implantation embryo development in mice

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: Feb. 28, 2025

Introduction Microplastics and nanoplastics are prevalent environmental contaminants. Recent reports indicate that polystyrene nanoparticles may adversely impact male reproductive health. This study aims to examine the effects of exposure on sperm metabolism development pre-implantation embryos. Methods In this study, C57BL/6 mice were orally gavage-administered (60 nm, 20 mg/kg/day) for 35 days assess their reproduction early embryonic development. Experiments included testicular transcriptome analysis, metabolomics, motility fertilization assays, ROS detection, RNA sequencing 2-cell embryos, revealing adverse embryo Results The results revealed oral gavage induced a pronounced immune-inflammatory response in tissue, reduced motility, significantly lowered rate. Notably, from treated exhibited substantial metabolic disruptions, affecting key pathways, including glycerophospholipid biosynthesis DNA repair. After fertilization, embryos at stage suffered damage apoptotic repair subsequently impairing Discussion conclusion, demonstrated administration affects function, resulting abnormalities alterations associated gene expression profiles. These findings offer essential scientific insights future research into sperm-mediated transgenerational

Language: Английский

Comparative Analysis of Volatile Organic Compounds in Different Parts of Ginseng Powder Using Gas Chromatography–Ion Mobility Spectrometry

Molecules, Journal Year: 2025, Volume and Issue: 30(9), P. 1965 - 1965

Published: April 29, 2025

The main root, reed head, and fibrous root are three different edible medicinal parts of ginseng (Panax C. A. Meyer). When processed into products, such as powder, they exhibit similar colors odors, easily confused in market circulation. However, there differences their pharmacological activity clinical indications. Therefore, the identification powder is crucial for ensuring quality, safety, efficacy products. In this study, we utilized gas chromatography–ion mobility spectrometry (GC–IMS) to analyze volatile organic components (VOCs) ginseng. It was found that composition VOCs similar, but content all samples, a total 68 signal peaks detected 65 identified. addition, combined with fingerprint analysis, principal component analysis (PCA), Euclidean distance, partial-least squares discriminant (PLS-DA), cluster (CA), it clearly showed significant between powder. Our findings reveal GC–IMS chemometrics reliable method distinguishing active provides essential data support processing functional product development.

Language: Английский