Colloids and Surfaces B Biointerfaces, Год журнала: 2025, Номер unknown, С. 114675 - 114675
Опубликована: Апрель 1, 2025
Язык: Английский
Translational Oncology, Год журнала: 2024, Номер 50, С. 102121 - 102121
Опубликована: Сен. 14, 2024
Язык: Английский
Процитировано
61
Advanced Drug Delivery Reviews, Год журнала: 2025, Номер 218, С. 115522 - 115522
Опубликована: Янв. 22, 2025
Язык: Английский
Процитировано
2
Frontiers in Bioengineering and Biotechnology, Год журнала: 2025, Номер 13
Опубликована: Фев. 19, 2025
Extracellular vesicles (EVs) are nano-sized, membranous structures secreted by cells into the extracellular space, have attracted considerable attention in field of biosciences for their role intercellular communication various physiological and pathological processes. Their ubiquitous presence bodily fluids cell-specific characteristics make them promising candidates as biomarkers. Additionally, ability to transport biological therapeutics across different barriers specific target underscores significant translational potential diagnostic therapeutic purposes. Significant progress has been achieved translation EVs research clinical applications, however, challenges persist large-scale production EVs, particularly areas scalable manufacturing, efficient isolation methods, drug loading techniques, advanced characterization technology. This review critically examines complex processes involved biogenesis explores recent developments production. By synthesizing knowledge from these fields, this aims provide a holistic perspective on evolving landscape its underscoring both accomplishments obstacles that lie ahead fully realizing biomedicine.
Язык: Английский
Процитировано
2
Pharmacological Research, Год журнала: 2024, Номер 204, С. 107202 - 107202
Опубликована: Май 3, 2024
Plant-derived extracellular vesicles (PDEV) constitute nanoscale entities comprising lipids, proteins, nucleic acids and various components enveloped by the lipid bilayers of plant cells. These play a crucial role in facilitating substance information transfer not only between cells but also across different species. Owing to its safety, stability, abundance raw materials, this has found extensive utilization recent years within research endeavors aimed at treating diseases. This article provides an overview pathways biological characteristics PDEV, along with prevalent methods employed for isolation, purification, storage. Furthermore, we comprehensively outline therapeutic implications diverse sources PDEV musculoskeletal system disorders. Additionally, explore as platforms engineering drug carriers, aiming delve deeper into significance potential contributions realm system.
Язык: Английский
Процитировано
8
Frontiers in Oncology, Год журнала: 2025, Номер 14
Опубликована: Янв. 16, 2025
Oral squamous cell carcinoma (OSCC) accounts for approximately 90% of all oral cancers, significantly impacting the survival and quality life patients. Exosomes, small extracellular vesicles released by cells, play a crucial role in intercellular communication cancer. Nevertheless, their function mechanism OSCC remain elusive. Search Pubmed, Web Science, Cochrane Library using keywords OSCC, exome, diagnosis, treatment to review research progress exome OSCC. Based on these results, this starting from biosynthesis, structure, contents exosomes, elaborates exosomes diagnosis It explores potential briefly describes challenges researchers currently face.
Язык: Английский
Процитировано
1
International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 140753 - 140753
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
1
Dentistry Journal, Год журнала: 2025, Номер 13(3), С. 106 - 106
Опубликована: Фев. 27, 2025
This review highlights the key molecular and cellular mechanisms contributing to aging, such as DNA damage, mitochondrial dysfunction, telomere shortening, protein defective autophagy. These biological are involved in various oral health conditions prevalent elderly, including periodontal disease, cancer, xerostomia, dental caries, temporomandibular joint disorders. Exosomes generated by mesenchymal stem cells possess substantial therapeutic potential. exosomes nanosized extracellular vesicles derived from essential intercellular communication tissue homeostasis. The exosome-based therapies proved superior traditional cell-based approaches, due lower immunogenicity, ease of storage, avoidance complications associated with cell transplantation. Furthermore, diagnostic potential non-invasive biomarkers for aging processes age-related diseases offers insights into disease diagnosis, staging, monitoring. Among challenges future perspectives translating exosome research preclinical studies clinical applications is need standardized procedures fully harness capabilities exosomes.
Язык: Английский
Процитировано
1
International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(5), С. 2211 - 2211
Опубликована: Фев. 28, 2025
Osteoarthritis, affecting over 8 million people in the UK, remains a debilitating condition with limited treatment options. Current therapies primarily address symptoms and can exacerbate joint damage time. Developing disease-modifying drugs that alleviate inflammation promote regeneration is crucial for long-term patient benefit. This study investigates potential of exosome-like nano-vesicles isolated from grapefruit juice (GEVs) as novel therapeutic approach osteoarthritis. GEVs possess regenerative properties present promising avenue clinical translation. In this study, were characterized terms protein quantification, size, morphology. vitro studies demonstrated safety efficacy GEVs, showing an enhancement human chondrocyte migratory activity 13%. exhibited dual mechanism action, reducing oxidative stress while promoting cellular regeneration. Specifically, they reduced expression COX2 PTGS2, markers associated pain sensitization, enhanced antioxidant genes SD2 GPX osteoarthritic-like chondrocytes. Additionally, downregulated ADAMTS-5 hypertrophic COL10 upregulating chondrogenic ACAN, COL2, SOX9. research signifies significant advancement osteoarthritis therapy, offering natural, safe, cost-effective option long-lasting benefits. Clinical translation GEV therapy holds promise improving outcomes burden on healthcare systems.
Язык: Английский
Процитировано
1
Preparative Biochemistry & Biotechnology, Год журнала: 2025, Номер unknown, С. 1 - 20
Опубликована: Март 14, 2025
Downstream processing continues to face significant bottlenecks due current purification technologies and improvements in upstream. Chromatography systems have been the primary method for their high yields purities. However, use of high-titer-producing strains has highlighted limitations chromatographic steps, including mass transfer limitations, low capacity, scalability issues. These challenges, combined with growing interest fully continuous manufacturing processes, led a widespread alternative affinity chromatography systems. Polyethylene glycol precipitation demonstrated be powerful, flexible, easily scalable, titer-independent methodology purifying therapeutic proteins such as monoclonal antibodies, achieving purities comparable Furthermore, it also holds great potential simplifying processes new modalities overcome downstream processing. Herein, we discuss latest advances polyethylene technology explore its future research directions applications.
Язык: Английский
Процитировано
1
Journal of Cellular and Molecular Medicine, Год журнала: 2025, Номер 29(8)
Опубликована: Апрель 1, 2025
ABSTRACT Sepsis‐induced cardiomyopathy (SICM) is a complex and fatal manifestation of sepsis, characterised by myocardial dysfunction that exacerbates the clinical prognosis in septic patients. While pathophysiology SICM remains incompletely understood, emerging evidence highlights multifaceted functions exosomes, small membrane‐bound extracellular vesicles, mediating inflammatory responses cardiac involved this condition. During exosomes are secreted various cells, such as cardiomyocytes, endothelial cells macrophages, which serve critical messengers, transferring proteins, lipids RNA molecules influence recipient thus affecting cellular disease progression. This review summarises basics focuses on exosome‐mediated mechanisms SICM, including their role inflammation, oxidative stress, mitochondrial injury, offering novel insights into exosome‐based therapeutic strategies SICM.
Язык: Английский
Процитировано
1