Hemolysis Derived Products Toxicity and Endothelium: Model of the Second Hit

Toxins, Год журнала: 2019, Номер 11(11), С. 660 - 660

Опубликована: Ноя. 13, 2019

Vascular diseases are multifactorial, often requiring multiple challenges, or ‘hits’, for their initiation. Intra-vascular hemolysis illustrates well the multiple-hit theory where a first event lyses red blood cells, releasing hemolysis-derived products, in particular cell-free heme which is highly toxic endothelium. Physiologically, derived-products rapidly neutralized by numerous defense systems, including haptoglobin and hemopexin scavenge hemoglobin heme, respectively. Likewise, cellular mechanisms involved, heme-oxygenase 1 upregulation metabolizes heme. However, cases of intra-vascular hemolysis, those systems overwhelmed. Heme exerts effects acting as damage-associated molecular pattern promoting, together with hemoglobin, nitric oxide scavenging ROS production. In addition, it activates complement coagulation systems. Together, these processes lead to endothelial cell injury triggers pro-thrombotic pro-inflammatory phenotypes. Moreover, among glomerular ones display susceptibility explained weaker capacity counteract injury. this review, we illustrate ‘multiple-hit’ through example focus on advance hypotheses explaining observed hemolytic diseases. Finally, describe therapeutic options reducing

Язык: Английский

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Recent Advances in the Microbial Synthesis of Hemoglobin

Trends in biotechnology, Год журнала: 2020, Номер 39(3), С. 286 - 297

Опубликована: Сен. 7, 2020

Язык: Английский

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Lessons from the post-genomic era: Globin diversity beyond oxygen binding and transport

Redox Biology, Год журнала: 2020, Номер 37, С. 101687 - 101687

Опубликована: Авг. 14, 2020

Vertebrate hemoglobin (Hb) and myoglobin (Mb) were among the first proteins whose structures sequences determined over 50 years ago. In subsequent pregenomic period, numerous related came to light in plants, invertebrates bacteria, that shared fold, a signature sequence motif characteristic of 3-on-3 α-helical sandwich. Concomitantly, eukaryote bacterial globins with truncated 2-on-2 fold discovered. Genomic information last 20 has dramatically expanded list known globins, demonstrating their existence limited number archaeal genomes, majority genomes an overwhelming genomes. vertebrates, 6 additional globin types identified, namely neuroglobin (Ngb), cytoglobin (Cygb), E (GbE), X (GbX), Y (GbY) androglobin (Adgb). Furthermore, functions beyond familiar oxygen transport storage have been discovered within vertebrate family, including NO metabolism, peroxidase activity, scavenging free radicals, signaling functions. The extension knowledge on suggests original roles must enzymatic, involved defense against toxicity, perhaps also as sensors O2, regulating taxis away or towards high O2 concentrations. this review, we aimed discuss evolution remarkable functional diversity particular focus variety non-canonical expression sites mammalian according impressive variability atypical

Язык: Английский

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Carbon Monoxide and Nitric Oxide as Examples of the Youngest Class of Transmitters

International Journal of Molecular Sciences, Год журнала: 2021, Номер 22(11), С. 6029 - 6029

Опубликована: Июнь 2, 2021

The year 2021 is the 100th anniversary of confirmation neurotransmission phenomenon by Otto Loewi. Over course hundred years, about 100 neurotransmitters belonging to many chemical groups have been discovered. In order celebrate neurotransmitters, we present an overview first two endogenous gaseous transmitters i.e., nitric oxide, and carbon monoxide, which are often termed as gasotransmitters.

Язык: Английский

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Systematic engineering of Saccharomyces cerevisiae for efficient synthesis of hemoglobins and myoglobins

Bioresource Technology, Год журнала: 2022, Номер 370, С. 128556 - 128556

Опубликована: Дек. 28, 2022

Язык: Английский

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Oxygen-releasing biomaterials for chronic wounds breathing: From theoretical mechanism to application prospect

Materials Today Bio, Год журнала: 2023, Номер 20, С. 100687 - 100687

Опубликована: Июнь 1, 2023

Chronic wounds have always been considered as "gordian knots" in medicine, which hypoxia plays a key role blocking healing. To address this challenge, although tissue reoxygenation therapy based on hyperbaric oxygen (HBOT) has performed clinically for several years, the bench to bedside still urges evolution of oxygen-loading and -releasing strategies with explicit benefits consistent outcome. The combination various carriers biomaterials gained momentum an emerging therapeutic strategy field, exhibiting considerable application potential. This review gives overview essential relationship between delayed wound Further, detailed characteristics, preparation methods applications oxygen-releasing (ORBMs) will be elaborated, including hemoglobin, perfluorocarbon, peroxide, oxygen-generating microorganisms, those are applied load, release or generate vast relieve hypoxemia bring subsequent cascade effect. pioneering papers regarding ORBMs practice presented trends toward hybrid more precise manipulation summarized.

Язык: Английский

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Hemoglobin Protects Enamel against Intrinsic Enamel Erosive Demineralization

Caries Research, Год журнала: 2024, Номер 58(2), С. 90 - 107

Опубликована: Янв. 1, 2024

<b><i>Introduction:</i></b> This study investigated the changes in acquired enamel pellicle (AEP) proteome when this integument is formed vivo after treatment with sugarcane-derived cystatin (CaneCPI-5), hemoglobin (HB), and a statherin-derived peptide (StN15), or their combination then exposed to an intrinsic acid challenge. The effectiveness of these treatments preventing erosion was also evaluated. <b><i>Methods:</i></b> Ten volunteers, prophylaxis, 5 crossover phases, rinsed following solutions (10 mL, 1 min): control (deionized water-H₂O) – group 1, 0.1 mg/mL CaneCPI-5 2, 1.0 HB 3, 1.88 × 10⁻⁵<sc>M</sc> StN15 4, blend 5. Following this, AEP formation occurred (2 h) biopsy µL, 0.01 <sc>m</sc> HCl, pH 2.0, 10 s) conducted on one incisor. analyzed for calcium (Arsenazo method). vestibular surfaces other teeth were treated same acid. Acid-resistant proteins residual collected quantitatively via proteomics. <b><i>Results:</i></b> Compared control, proteins/peptide, mixed isolated, markedly enhanced acid-resistant AEP. Notable increases pyruvate kinase PKM (11-fold, CaneCPI-5), immunoglobulins submaxillary gland androgen-regulated protein 3B (4-fold, StN15), Hb, lysozyme C (2-fold, StN15). Additionally, range not commonly identified but known bind groups tested proteins/peptide either isolation as mixture. mean (SD, m<sc>M</sc>) concentrations released from 3.67 ± 1.48a, 3.11 0.72a, 1.94 0.57b, 2.37 0.90a, 2.38 0.45a 1–5, respectively (RM-ANOVA/Tukey, <i>p</i> &lt; 0.05). <b><i>Conclusions:</i></b> Our findings demonstrate that all treatments, whether using proteins/peptides isolation, However, only showed protecting against erosive demineralization. These results pave way innovative preventive methods erosion, “acquired engineering” techniques.

Язык: Английский

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Analytic and In Silico Methods to Understand the Interactions between Dinotefuran and Haemoglobin

Chemistry & Biodiversity, Год журнала: 2024, Номер 21(8)

Опубликована: Июнь 5, 2024

This work lies in the growing concern over potential impacts of pesticides on human health and environment. Pesticides are extensively used to protect crops control pests, but their interaction with essential biomolecules like haemoglobin (Hb) remains poorly understood. Spectrofluorometric, electrochemical, silico investigations have been chosen as methods delve into this issue, they offer valuable insights molecular-level interactions between haemoglobin. The research aims address gaps knowledge contribute developing safer more sustainable pesticide practices. was studied by spectroscopic techniques (UV-Visible & Fluorescence), studies (molecular docking molecular dynamics simulations) electrochemical (cyclic voltammetry tafel). showed effective binding dinotefuran Hb which will cause toxicity human. formation a stable complex ofloxacin Haemoglobin shown via energy found be -5.37 kcal/mol. Further, simulations provide an insight for stability (Hb-dinotefuran) span 250 ns free -53.627 kJ/mol. cyclic tafel show effectively.

Язык: Английский

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Hemoglobin mediates the link between the ‘weekend warrior’ physical activity pattern and diabetic retinopathy

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 8, 2025

Diabetic retinopathy (DR), the leading cause of vision loss in elderly individuals, coupled with limited treatment options, has prompted efforts to identify modifiable risk factors associated DR. The purpose this study was explore associations between "weekend warrior" (WW) physical activity (PA) pattern and DR U.S. adults examine how Hb levels mediate relationship. Cross-sectional data were obtained from nationally representative NHANES 2007 2018. PA categorized as inactive, insufficiently active, WW, or regularly active (RA). ultimately included 6145 adults, including 1043 participants 5102 DM but not Multivariate logistic regression modelling indicated that both WW (OR = 0.631, 95% CI 0.487–0.818, P < 0.001) RA 0.738, 0.574–0.959, 0.018) patterns significant protective against compared inactive pattern. Moreover, patterns, did show a association DR, whereas 1.355, 1.054–1.742, factor. Mediation analysis revealed partial mediation effect level on risk, ratio 5.95%. Our are association. In comparison, is most cost effective for prevention.

Язык: Английский

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Study in the iron uptake mechanism of Pasteurella multocida

Veterinary Research, Год журнала: 2025, Номер 56(1)

Опубликована: Фев. 13, 2025

Abstract Pasteurella multocida infects a wide range of animals, causing hemorrhagic septicemia or infectious pneumonia. Iron is an essential nutrient for growth, colonization, and proliferation P. during infection the host, competition iron ions in host critical link pathogenesis this pathogen. In recent years, there has been significant progress study uptake system , including its occurrence regulatory mechanisms. order to provide systematic theoretical basis molecular system, generate new ideas investigation development molecular-targeted drugs subunit vaccines against mechanisms by transferrin receptors, heme siderophores, mechanism expression regulation are all described.

Язык: Английский

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